Syringocistadenocarcinoma papilliferum

Se trata de una paciente de 54 años que comenzó con una lesión en la vulva a nivel de la cicatriz de la rafia desde hace cuatro años aproximadamente que evolucionó a un absceso, para lo que llevó tratamiento en varias ocasiones, sin mejoría, por lo que continuó con sangrado y dolor en la región de la vulva. Decidió acudir a la Consulta ginecológica del Hospital “Mariana Grajales” de Santa Clara y se le diagnosticó lesión en horquilla vulvar de 3cm, sobreelevada, de bordes indurados y ulcerada. Por las características clínicas de la lesión se decidió su ingreso en la Sala de Ginecología y se le realizó una vulvectomía con linfadenectomía inguinal bilateral. El diagnóstico histológico consistió en un siringocistadenocarcinoma papilífero ulcerado infiltrante de la vulva. El siringocistadenocarcinoma papilífero es un tumor anexial maligno derivado de las glándulas sudoríparas extremadamente infrecuente. Se localiza, predominantemente, en la cabeza y el cuello y puede desarrollarse de un siringocistadenoma papilífero o de un nevo sebáceo de Jadassohn; su localización en la vulva es infrecuente.This is a 54-year-old patient who started with an injury to the vulva at the level of the scar of the raffia for about four years, which evolves and abscess, what has been treated several times, without improvement, so it continued with bleeding and pain in the region of the vulva. He decided to go to the Gynecological Consultation of the Mariana Grajales Hospital of Santa Clara, and she was diagnosed with a lesion in the vulvar fork of 3 cm, raised, with indurated and ulcerated edges. The clinical characteristics of the lesion are resolved in the Gynecology Room and a vulvectomy is performed with bilateral inguinal lymphadenectomy. The histological diagnosis consisted of a syringocistadenocarcinoma of the ulcerated infiltrating papillary vulva. Syringocistadenocarcinoma papilliferum is a malignant tumor derived from the sweat glands extremely infrequent. It is located, predominantly, in the head and neck and may develop a papilliferous syringocistadenoma or a sebaceous neva of Jadassohn; Its location in the vulva is uncommon

Uterine perivascular epithelial cell tumor (PEComa), a rare tumor

Se trata de una paciente de 47 años de edad, de raza blanca, con antecedentes de ser sordo-muda y de retraso mental así como de padecer diabetes mellitus tipo 2 e hipertensión arterial esencial, para lo que lleva tratamiento. En esta ocasión acudió a su área de salud por sangramiento vaginal de seis meses de evolución; le realizaron examen físico y ultrasonido ginecológico y le diagnosticaron un fibroma uterino, por lo que fue remitida al Hospital Ginecoobstétrico “Mariana Grajales” para ser intervenida quirúrgicamente. En el acto quirúrgico la maniobra de extracción del cuello uterino fue difícil; fue recibido en varios fragmentos. El estudio anatomopatológico informó la presencia en el cuello uterino de un tumor de células epitelioides perivascular (PEComa). Los PEComas son una familia de tumores de origen mesenquimal con características citomorfológicas e inmunohistoquímicas similares dadas por la presencia de células epiteliodes y fusocelulares con citoplasma eosinófilo o claro que tienen íntima relación con la vasculatura y característicamente co-expresan marcadores melanocíticos y mioides. Son tumores extremadamente raros que, debido a su disposición perivascular, pueden ubicarse en cualquier parte del cuerpo (en la literatura se describen en diversas partes), así como afectar a cualquier edad o sexo; aunque llama la atención su mayor predisposición hacia el sexo femenino.A female patient of 47 year olds, of white race, with a history of being deaf-mute and mentally retarded as well as having type 2 diabetes mellitus and essential hypertension for which she is being treated. On this occasion she went to her health area due to vaginal bleeding of six months of evolution; Performed a physical examination and gynecological ultrasound and were diagnosed with a uterine fibroma, so she was referred to the Gynecological Obstetrical Hospital “Mariana Grajales” to undergo surgery. In the surgical act the removal of the cervix was difficult; Was received in several fragments. The anatomopathological study reported the presence in the cervix of a perivascular epithelial cell tumor (PEComa). PEComas are a family of tumors of mesenchymal origin with similar cytomorphological and immunohistochemical characteristics due to the presence of epithelioid and fusocellular cells with eosinophilic or clear cytoplasm that are closely related to the vasculature and characteristically co-express melanocytic and myoid markers. They are extremely rare tumors that, due to their perivascular disposition, can be located in any part of the body (in the literature they are described in diverse parts of the organism), as well as to affect any age or sex; although it draws attention its greater predisposition towards the female sex

Low-grade endometrial stromal sarcoma

Se trata de una paciente de 25 años con historia de salud anterior e historia obstétrica de un embarazo y un parto. En esta ocasión acudió a consulta por presentar dolor bajo vientre desde hacía tres meses y expulsión por la vagina de un material muy fétido desde hacía quince días. Al examen físico se observó una tumoración de color blanquecino grisáceo que salía por el orificio cervical externo, con superficie reblandecida, de la que se tomó una muestra para estudio histológico, pero no fue útil para diagnóstico, por lo que se impuso tratamiento antibiótico; luego de otra toma de muestra para biopsia, que no fue concluyente para diagnóstico, se decidió realizar histerectomía con doble anexectomía y omentectomía. El diagnóstico histopatológico resultó ser un sarcoma del estroma endometrial de bajo grado. El sarcoma del estroma endometrial es un tumor infrecuente que contabiliza menos del 10% de los sarcomas uterinos. Dentro de los tumores mesenquimales uterinos se encuentran los tumores del estroma endometrial, que a su vez se dividen en nódulo estromal endometrial (benigno, bien circunscrito y sin invasión vascular) y el sarcoma del estroma endometrial, antes denominado miosis estromal endolinfática, que representa el 0,25% de los tumores malignos uterinos.She is a 25-year-old patient with a previous health history, an obstetric history of pregnancy and childbirth. On this occasion she went to the clinic for presenting pain in the abdomen for three months and expulsion from the vagina of a very fetid material for fifteen days. On physical examination, a gray-white tumor was seen that exited through the external cervical orifice, with a softened area, from which a sample was taken for histological study, but it was not useful for diagnosis, for which antibiotic treatment was applied; after another biopsy sample, which was not conclusive for diagnosis, it was decided to perform a hysterectomy with double adnexectomy and omentectomy. The histopathological diagnosis was found to be a low-grade endometrial stromal sarcoma. Endometrial stromal sarcoma is an infrequent tumor that accounts for less than 10% of uterine sarcomas. Within the uterine mesenchymal tumors are tumors of the endometrial stroma, which in turn are divided into endometrial stromal nodules (benign, well circumscribed and without vascular invasion) and endometrial stromal sarcoma, formerly called stromal miosis endolymphatic, which represents 0.25% of uterine malignancies

OMEGA - OSIRIS Mapping of Emission-line Galaxies in A901/2: I. Survey description, data analysis, and star formation and AGN activity in the highest density regions

We present an overview of and first results from the OMEGA (OSIRIS Mapping of Emission-line Galaxies in the multicluster system A901/2) survey. The ultimate goal of this project is to study star formation and active galactic nucleus (AGN) activity across a broad range of environments at a single redshift. Using the tuneable-filter mode of the Optical System for Imaging and low-Intermediate-Resolution Integrated Spectroscopy (OSIRIS) instrument on Gran Telescopio Canarias, we target Hα and [NII] emission lines over an ∼0.5×0.5 deg2 region containing the z∼0.167 multicluster system A901/2. In this paper, we describe the design of the survey, the observations and the data analysis techniques developed. We then present early results from two OSIRIS pointings centred on the cores of the A901a and A902 clusters. AGN and star-forming (SF) objects are identified using the [NII]/Hα versus WHα diagnostic diagram. The AGN hosts are brighter, more massive, and possess earlier type morphologies than SF galaxies. Both populations tend to be located towards the outskirts of the high-density regions we study. The typical Hα luminosity of these sources is significantly lower than that of field galaxies at similar redshifts, but greater than that found for A1689, a rich cluster at z∼0.2. The Hα luminosities of our objects translate into star formation rates (SFRs) between ∼0.02 and 6 Myr−1. Comparing the relationship between stellar mass and Hα-derived SFR with that found in the field indicates a suppression of star formation in the cores of the clusters. These findings agree with previous investigations of this multicluster structure, based on other star formation indicators, and demonstrate the power of tuneable filters for this kind of study

Spatial and Temporal Variations in the Annual Pollen Index Recorded by Sites Belonging to the Portuguese Aerobiology Network

This study presents the findings of a 10-year survey carried out by the Portuguese Aerobiology Network (RPA) at seven pollen-monitoring stations: five mainland stations (Oporto, Coimbra, Lisbon, Évora and Portimão) and two insular stations [Funchal (Madeira archipelago) and Ponta Delgada (Azores archipelago)]. The main aim of the study was to examine spatial and temporal variations in the Annual Pollen Index (API) with particular focus on the most frequently recorded pollen types. Pollen monitoring (2003–2012) was carried out using Hirst-type volumetric spore traps, following the minimum recommendations proposed by the European Aerobiology Society Working Group on Quality Control. Daily pollen data were examined for similarities using the Kruskal–Wallis nonparametric test and multivariate regression trees. Simple linear regression analysis was used to describe trends in API. The airborne pollen spectrum at RPA stations is dominated by important allergenic pollen types such as Poaceae, Olea and Urticaceae. Statistically significant differences were witnessed in the API recorded at the seven stations. Mean API is higher in the southern mainland cities, e.g. Évora, Lisbon and Portimão, and lower in insular and littoral cities. There were also a number of significant trends in API during the 10-year study. This report identifies spatial and temporal variations in the amount of airborne pollen recorded annually in the Portuguese territory. There were also a number of significant changes in API, but no general increases in the amount of airborne pollen

Functional and Transcriptional Induction of Aquaporin-1 Gene by Hypoxia; Analysis of Promoter and Role of Hif-1α

Aquaporin-1 (AQP1) is a water channel that is highly expressed in tissues with rapid O2 transport. It has been reported that this protein contributes to gas permeation (CO2, NO and O2) through the plasma membrane. We show that hypoxia increases Aqp1 mRNA and protein levels in tissues, namely mouse brain and lung, and in cultured cells, the 9L glioma cell line. Stopped-flow light-scattering experiments confirmed an increase in the water permeability of 9L cells exposed to hypoxia, supporting the view that hypoxic Aqp1 up-regulation has a functional role. To investigate the molecular mechanisms underlying this regulatory process, transcriptional regulation was studied by transient transfections of mouse endothelial cells with a 1297 bp 5′ proximal Aqp1 promoter-luciferase construct. Incubation in hypoxia produced a dose- and time-dependent induction of luciferase activity that was also obtained after treatments with hypoxia mimetics (DMOG and CoCl2) and by overexpressing stabilized mutated forms of HIF-1α. Single mutations or full deletions of the three putative HIF binding domains present in the Aqp1 promoter partially reduced its responsiveness to hypoxia, and transfection with Hif-1α siRNA decreased the in vitro hypoxia induction of Aqp1 mRNA and protein levels. Our results indicate that HIF-1α participates in the hypoxic induction of AQP1. However, we also demonstrate that the activation of Aqp1 promoter by hypoxia is complex and multifactorial and suggest that besides HIF-1α other transcription factors might contribute to this regulatory process. These data provide a conceptual framework to support future research on the involvement of AQP1 in a range of pathophysiological conditions, including edema, tumor growth, and respiratory diseases

Assessment of a New ROS1 Immunohistochemistry Clone (SP384) for the Identification of ROS1 Rearrangements in Patients with Non–Small Cell Lung Carcinoma: the ROSING Study

Introduction: The ROS1 gene rearrangement has become an important biomarker in NSCLC. The College of American Pathologists/International Association for the Study of Lung Cancer/Association for Molecular Pathology testing guidelines support the use of ROS1 immunohistochemistry (IHC) as a screening test, followed by confirmation with fluorescence in situ hybridization (FISH) or a molecular test in all positive results. We have evaluated a novel anti-ROS1 IHC antibody (SP384) in a large multicenter series to obtain real-world data. Methods: A total of 43 ROS1 FISH-positive and 193 ROS1 FISH-negative NSCLC samples were studied. All specimens were screened by using two antibodies (clone D4D6 from Cell Signaling Technology and clone SP384 from Ventana Medical Systems), and the different interpretation criteria were compared with break-apart FISH (Vysis). FISH-positive samples were also analyzed with next-generation sequencing (Oncomine Dx Target Test Panel, Thermo Fisher Scientific). Results: An H-score of 150 or higher or the presence of at least 70% of tumor cells with an intensity of staining of 2+ or higher by the SP384 clone was the optimal cutoff value (both with 93% sensitivity and 100% specificity). The D4D6 clone showed similar results, with an H-score of at least 100 (91% sensitivity and 100% specificity). ROS1 expression in normal lung was more frequent with use of the SP384 clone (p < 0.0001). The ezrin gene (EZR)-ROS1 variant was associated with membranous staining and an isolated green signal FISH pattern (p = 0.001 and p = 0.017, respectively). Conclusions: The new SP384 ROS1 IHC clone showed excellent sensitivity without compromising specificity, so it is another excellent analytical option for the proposed testing algorithm

Search for new particles in events with energetic jets and large missing transverse momentum in proton-proton collisions at root s=13 TeV

A search is presented for new particles produced at the LHC in proton-proton collisions at root s = 13 TeV, using events with energetic jets and large missing transverse momentum. The analysis is based on a data sample corresponding to an integrated luminosity of 101 fb(-1), collected in 2017-2018 with the CMS detector. Machine learning techniques are used to define separate categories for events with narrow jets from initial-state radiation and events with large-radius jets consistent with a hadronic decay of a W or Z boson. A statistical combination is made with an earlier search based on a data sample of 36 fb(-1), collected in 2016. No significant excess of events is observed with respect to the standard model background expectation determined from control samples in data. The results are interpreted in terms of limits on the branching fraction of an invisible decay of the Higgs boson, as well as constraints on simplified models of dark matter, on first-generation scalar leptoquarks decaying to quarks and neutrinos, and on models with large extra dimensions. Several of the new limits, specifically for spin-1 dark matter mediators, pseudoscalar mediators, colored mediators, and leptoquarks, are the most restrictive to date.Peer reviewe

Probing effective field theory operators in the associated production of top quarks with a Z boson in multilepton final states at root s=13 TeV

Peer reviewe

Combined searches for the production of supersymmetric top quark partners in proton-proton collisions at root s=13 TeV

A combination of searches for top squark pair production using proton-proton collision data at a center-of-mass energy of 13 TeV at the CERN LHC, corresponding to an integrated luminosity of 137 fb(-1) collected by the CMS experiment, is presented. Signatures with at least 2 jets and large missing transverse momentum are categorized into events with 0, 1, or 2 leptons. New results for regions of parameter space where the kinematical properties of top squark pair production and top quark pair production are very similar are presented. Depending on themodel, the combined result excludes a top squarkmass up to 1325 GeV for amassless neutralino, and a neutralinomass up to 700 GeV for a top squarkmass of 1150 GeV. Top squarks with masses from 145 to 295 GeV, for neutralino masses from 0 to 100 GeV, with a mass difference between the top squark and the neutralino in a window of 30 GeV around the mass of the top quark, are excluded for the first time with CMS data. The results of theses searches are also interpreted in an alternative signal model of dark matter production via a spin-0 mediator in association with a top quark pair. Upper limits are set on the cross section for mediator particle masses of up to 420 GeV