Efecto de los 4-pregnenos (derivados de la progesterona) sobre el comportamiento biológico de líneas celulares derivadas tumores de ovario

Comunicación científica en formato ORAL, realizada en las III Jornadas Internacionales de Investigación, Ciencia y Universidad y las XII Jornadas de Investigación UMaza, en el Bloque de comunicaciones científicas: "BIOLOGÍA MOLECULAR, BIOQUÍMICA E INMUNOLOGÍA", el mismo fue moderado por el Dr. CRISTIAN QUINTERO. Las jornadas se llevaron adelante desde 19 al 23 de octubre del 2020 en formato totalmente virtual bajo plataforma Zoom y fueron transmitidas por el canal YouTube de la UMaza y el Facebook del Área de Ciencia y Técnica UMaza (Somos Ciencia y Técnica UMaza)

Original Article Biomarkers of erlotinib response in non-small cell lung cancer tumors that do not harbor the more common epidermal growth factor receptor mutations

Abstract: Non-small cell lung cancer (NSCLC) represents approximately 85% of all lung cancers, which are the leading cause of cancer-related deaths in the world. Tyrosine kinase inhibitors such as erlotinib represent one therapeutic options presently recommended for tumors produced by activating mutations in the gene coding of epidermal growth factor receptor (EGFR). The aim of this study is the identification of possible biomarkers for tumor sensitivity to erlotinib in the absence of the main EGFR mutations. The erlotinib sensitivity of cells isolated from 41 untreated NSCLC patients was determined and compared with the presence of the more frequent EGFR mutations. Several patients had tumor cells highly sensitive to erlitinib in the absence of the EGFR mutations analyzed. The gene expression profile of 3 erlotinib-sensitive tumors was compared with that of 4 resistant tumors by DNA microarray hybridization. Sixteen genes were expressed at significantly higher levels in the resistant tumors than in the sensitive tumors. The possible correlation between erlotinib sensitivity and the expression of these genes was further analyzed using the data for the NSCLC, breast cancer and colon cancer cell lines of the NCI60 collection. The expression of these genes was correlated with the overall survival of 5 patients treated with erlotinib, according to The Cancer Genome Atlas (TCGA) database. Overlapping groups of 7, 5 and 3 genes, including UGT1A6, TRIB3, MET, MMP7, COL17A1, LCN2 and PTPRZ1, whose expression correlated with erlotinib activity was identified. In particular, low MET expression levels showed the strongest correlation

Fichas de aprendizaje de Comunicación 2

Proponemos trabajar las seis fichas de forma flexible y libre, con el compromiso de que tú y tu docente planifiquen y desarrollen las actividades propuestas. Al interior de las fichas, hallarás una situación de la vida real o simulada que implica resolver un reto que te acercará a diversos contextos del Perú y del mundo. En ese sentido, ponemos a tu disposición diversos tipos y géneros textuales que, mediante la realización de actividades, elaboración de productos y evidencias, potenciarán el desarrollo de tus competencias comunicativas. En cada ficha se propone la siguiente secuencia: primero, te presentamos una situación con preguntas a modo de reto; seguidamente, te invitamos a leer la diversidad de textos propuestos, los cuales te permitirán involucrarte en los temas planteados para comprenderlos e interpretarlos, y con los que podrás desarrollar las actividades de lectura —por medio de las cuales queremos que profundices tu comprensión—, y otras dirigidas a la elaboración de los productos orales y escritos

Growth And The Growth Hormone-Insulin Like Growth Factor 1 Axis In Children With Chronic Inflammation:Current Evidence, Gaps In Knowledge And Future Directions

Growth failure is frequently encountered in children with chronic inflammatory conditions like juvenile idiopathic arthritis, inflammatory bowel disease and cystic fibrosis. Delayed puberty and attenuated pubertal growth spurt is often seen during adolescence. The underlying inflammatory state mediated by pro-inflammatory cytokines, prolonged use of glucocorticoid and suboptimal nutrition contribute to growth failure and pubertal abnormalities. These factors can impair growth by their effects on the growth hormone-insulin like growth factor axis and also directly at the level of the growth plate via alterations in chondrogenesis and local growth factor signaling. Recent studies on the impact of cytokines and glucocorticoid on the growth plate studies further advanced our understanding of growth failure in chronic disease and provided a biological rationale of growth promotion. Targeting cytokines using biologic therapy may lead to improvement of growth in some of these children but approximately one third continue to grow slowly. There is increasing evidence that the use of relatively high dose recombinant human growth hormone may lead to partial catch up growth in chronic inflammatory conditions, although long term follow-up data is currently limited. In this review, we comprehensively review the growth abnormalities in children with juvenile idiopathic arthritis, inflammatory bowel disease and cystic fibrosis, systemic abnormalities of the growth hormone-insulin like growth factor axis and growth plate perturbations. We also systematically reviewed all the current published studies of recombinant human growth hormone in these conditions and discuss the role of recombinant human insulin like growth factor-1

Role of Dusp6 Phosphatase as a Tumor Suppressor in Non-Small Cell Lung Cancer

DUSP6/MKP3 is a dual-specific phosphatase that regulates extracellular regulated kinase ERK1/2 and ERK5 activity, with an increasingly recognized role as tumor suppressor. In silico studies from Gene expression Omnibus (GEO) and Cancer Genome atlas (TCGA) databases reveal poor prognosis in those Non-small cell lung cancer (NSCLC) patients with low expression levels of DUSP6. In agreement with these data, here we show that DUSP6 plays a major role in the regulation of cell migration, motility and tumor growth. We have found upregulation in the expression of several genes involved in epithelial to mesenchymal transition (EMT) in NSCLC-DUSP6 depleted cells. Data obtained in RNA-seq studies carried out in DUSP6 depleted cells identified EGFR, TGF-β and WNT signaling pathways and several genes such as VAV3, RUNXR2, LEF1, FGFR2 whose expression is upregulated in these cells and therefore affecting cellular functions such as integrin mediated cell adhesion, focal adhesion and motility. Furthermore, EGF signaling pathway is activated via ERK5 and not ERK1/2 and TGF-β via SMAD2/3 in DUSP6 depleted cells. In summary DUSP6 is a tumor suppressor in NSCLC and re-establishment of its expression may be a potential strategy to revert poor outcome in NSCLC patients

Cuaderno de trabajo de Comunicación, comprensión lectora 2 : segundo grado de Secundaria

El Cuaderno de trabajo de Comunicación Comprensión lectora 2 que ponemos en tus manos ha sido elaborado con el propósito de ayudarte a desarrollar tus competencias comunicativas, es decir, la lectura, la escritura y la comunicación oral. Por medio del trabajo con los textos que aquí te presentamos, podrás acceder a diversos conocimientos, ideas, experiencias y sentimientos del género humano, a partir de distintas situaciones de la vida cotidiana. Este material ha sido organizado en seis fichas, cada una de las cuales aborda algún tópico que recoge una problemática actual. Dentro de cada ficha, hallarás una situación de la vida real o simulada que implica un reto por afrontar y que te acercará a diversos contextos del Perú y del mundo. Mediante el desarrollo de las actividades y tareas que te proponemos, podrás elaborar productos que evidenciarán el desarrollo de tus competencias comunicativas. De este modo, encontrarás textos de diferentes géneros en cada ficha, con los cuales buscamos que te acerques y familiarices con una diversidad de tipos textuales. Por medio de la lectura interactiva, te involucrarás en la temática planteada para comprenderla y emprender la resolución del desafío de la ficha. Luego de los textos, te presentamos algunas actividades de lectura, mediante las cuales queremos que profundices tu comprensión, y otras dirigidas a la elaboración de los productos orales y escritos, que favorecerán el desarrollo de tus competencias de escritura y oralidad. Por otro lado, te invitamos a ampliar la información por medio de la investigación y a leer otros textos que complementen los que aquí te proponemos, con el objetivo de que tengas un mayor sustento y más ideas que te ayuden a resolver los retos de las fichas y a desarrollar los productos planteados

Multidimensional inhibitory signatures of sentential negation in behavioral variant frontotemporal dementia

Background: Processing of linguistic negation has been associated to inhibitory brain mechanisms. However, no study has tapped this link via multimodal measures in patients with core inhibitory alterations, a critical approach to reveal direct neural correlates and potential disease markers. Methods: Here we examined oscillatory, neuroanatomical, and functional connectivity signatures of a recently reported Go/No-go negation task in healthy controls and behavioral variant frontotemporal dementia (bvFTD) patients, typified by primary and generalized inhibitory disruptions. To test for specificity, we also recruited persons with Alzheimer's disease (AD), a disease involving frequent but nonprimary inhibitory deficits. Results: In controls, negative sentences in the No-go condition distinctly involved frontocentral delta (2-3 Hz) suppression, a canonical inhibitory marker. In bvFTD patients, this modulation was selectively abolished and significantly correlated with the volume and functional connectivity of regions supporting inhibition (e.g. precentral gyrus, caudate nucleus, and cerebellum). Such canonical delta suppression was preserved in the AD group and associated with widespread anatomo-functional patterns across non-inhibitory regions. Discussion: These findings suggest that negation hinges on the integrity and interaction of spatiotemporal inhibitory mechanisms. Moreover, our results reveal potential neurocognitive markers of bvFTD, opening a new agenda at the crossing of cognitive neuroscience and behavioral neurology.Fil: Díaz Rivera, Mariano Nicolás. Universidad de San Andrés; ArgentinaFil: Birba, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Neurociencia Cognitiva. Fundación Favaloro. Instituto de Neurociencia Cognitiva; ArgentinaFil: Fittipaldi, Sol. Universidad de San Andrés; ArgentinaFil: Mola, Débora Jeanette. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; ArgentinaFil: Morera Cáceres, Yurena. Universidad de La Laguna; EspañaFil: de Vega, Manuel. Universidad de La Laguna; EspañaFil: Moguilner, Sebastián. Universidad Adolfo Ibañez; ChileFil: Lillo, Patricia. Universidad de Chile; ChileFil: Slachevsky, Andrea. No especifíca;Fil: Gonzalez Campo, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Neurociencia Cognitiva. Fundación Favaloro. Instituto de Neurociencia Cognitiva; ArgentinaFil: Ibañez, Agustin Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de San Andrés; Argentina. University of California; Estados Unidos. Universidad Adolfo Ibañez; ChileFil: García, Adolfo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Educación Elemental y Especial; Argentina. Universidad de San Andrés; Argentin