Wetland restoration and nitrate reduction: the example of the periurban wetland of Vitoria-Gasteiz (Basque Country, North Spain)

Changes in land use and agricultural intensification caused wetlands on the quaternary aquifer of Vitoria-Gasteiz (Basque Country) to disappear some years ago and nitrate concentration in groundwaters increased very quickly. The Basque Government recently declared the East Sector of this aquifer a Vulnerable Zone according to the 91/676/CEE European Directive. Recently, the wetlands have been restored through the closure of the main drainage ditches, the consequent elevation of the water table and the abondonment of agricultural practices near the wetlands. This is the case of the Zurbano wetland. Restoration has allowed the recovery of its biogeochemical function, which has reduced nitrate concentrations in waters. Nitrate concentrations which exceed 50 mg l–1 in groundwaters entering into the wetland are less than 10 mg l–1 at the outlet. Conditions in the wetland are conducive to the loss of nitrates: organic matter rich wetted soils, clay presence allowing a local semiconfined flow and very low hydraulic gradient. Water quality monitoring at several points around the wetland showed the processes involved in nitrate loss, although some aspects still remain unresolved. However, during storm events, the wetland effectively reduces the nitrate concentration entering the Alegria River, the most important river on the quaternary aquifer

Sensitivity to anti-Fas is independent of increased cathepsin D activity and adrenodoxin reductase expression occurring in NOS-3 overexpressing HepG2 cells

© 2015 Elsevier B.V. Stable overexpression of endothelial nitric oxide synthase (NOS-3) in HepG2 cells (4TO-NOS) leads to increased nitro-oxidative stress and upregulation of the cell death mediators p53 and Fas. Thus, NOS-3 overexpression has been suggested as a useful antiproliferative mechanism in hepatocarcinoma cells. We aimed to identify the underlying mechanism of cell death induced by NOS-3 overexpression at basal conditions and with anti-Fas treatment. The intracellular localization of NOS-3, the nitro-oxidative stress and the mitochondrial activity were analysed. In addition, the protein expression profile in 4TO-NOS was screened for differentially expressed proteins potentially involved in the induction of apoptosis. NOS-3 localization in the mitochondrial outer membrane was not associated with changes in the respiratory cellular capacity, but was related to the mitochondrial biogenesis increase and with a higher protein expression of mitochondrial complex IV. Nitro-oxidative stress and cell death in NOS-3 overexpressing cells occurred with the expression increase of pro-apoptotic genes and a higher expression/activity of the enzymes adrenodoxin reductase mitochondrial (AR) and cathepsin D (CatD). CatD overexpression in 4TO-NOS was related to the apoptosis induction independently of its catalytic activity. In addition, CatD activity inhibition by pepstatin A was not effective in blocking apoptosis induced by anti-Fas. In summary, NOS-3 overexpression resulted in an increased sensitivity to anti-Fas induced cell death, independently of AR expression and CatD activity.Instituto de Salud Carlos III (FIS 09/00185). G. Ferrín was supported by the Networked Biomedical Research Center Hepatic and Digestive Diseases (CIBEREHD)Peer Reviewe

Integrating dual C and N isotopic approach to elemental and mathematical solutions for improving the PM source apportionment in complex urban and industrial cities: Case of Tarragona - Spain

Identification of dominant airborne Particulate Matter (PM) sources is essential for maintaining high air quality standards and thus ensuring a good public health. In this study, different approaches were applied for source apportionment of three PM fractions (PM1, PM2.5 and PM10) at the outdoor of 14 schools of a coastal city with a significant land use interweaving such as Tarragona (Spain). PM were collected in 24h-quartz microfiber filters in two seasonal campaigns (cold and warm), together with nine local potential sources, so a total of 84 samples were chemically, mineralogically, and isotopically characterised. Source apportionment was assessed by (i) main chemical components, (ii) Principal Component Analysis (PCA), (iii) dual C and N isotope approach, and (iv) a Bayesian isotope mixing model. When chemical concentrations were grouped into marine, crustal, secondary inorganic aerosols and organic matter + elemental carbon categories, the unaccounted component reached 45% of PM mass. The PCA allowed to identify also traffic and industrial contributions, reducing the unaccounted mass to about 25%. Adding δ13C and δ15N values, secondary organic aerosol could be estimated and a continuous contribution of diesel combustion was identified together with a remarkable use of natural gas in winter. Isotopic values were better understood when considering air masses back trajectories and a possible long-distance contribution from coal-fired electric generating units (EGUs). Finally, using Bayesian dual isotope mixing models, the unaccounted PM mass was reduced up to 5% when adding these EGUs to marine-carbonate related, road traffic, domestic heating, waste incinerator and livestock waste contributions. The added value of the dual isotope approach combined with a Bayesian isotope mixing model, in comparison with conventional chemical approaches, was thus demonstrated for PM source apportionment in an urban and industrial site where many sources and processes converge and can then be applied to other complex cities

Population vulnerability to extreme cold days in rural and urban municipalities in ten provinces in Spain

Background: The objective was to analyze whether there are differences in vulnerability to Extreme Cold Days (ECD) between rural and urban populations in Spain. Methodology: Time series analysis carried out from January 1, 2000, through December 31, 2013. Municipalities with over 10,000 inhabitants were included from 10 Spanish provinces, classified into 42 groups by isoclimate and urban/rural character as defined by Eurostat criteria. The statistical strategy was carried out in two phases. First: It was analyzed the relationship between minimum daily temperature (Tmin) (source: AEMET) and the rate of daily winter mortality due to natural causes —CIE-10: A00 – R99— (source: National Statistics Institute). Then, It was determinated the threshold of Tmin that defines the ECD and its percentile in the series of winter Tmin (Pthreshold), which is a measure of vulnerability to ECD so that the higher the percentile, the higher the vulnerability. Second: possible explanatory variables of vulnerability were explored using Mixed Generalized Models, using 13 independent variables related to meteorology, environment, socioeconomics, demographics and housing quality. Results: The average Pthreshold was 18 %. The final model indicated that for each percentage point increase in unemployment, the vulnerability to ECD increased by 0.4 (0.2, 0.6) points. Also, with each point increase in rurality index, this vulnerability decreased by −6.1 (−2.1, −10.0) points. Although less determinant, other factors that could contribute to explaining vulnerability at the province level included minimum winter daily temperatures and the percentage of housing with poor insulation. Conclusions: The vulnerability to ECD was greater in urban zones than in rural zones. Socioeconomic status is a key to understanding how this vulnerability is distributed. These results suggest the need to implement public health prevention plans to address ECD at the state level. These plans should be based on threshold temperatures determined at the smallest scale possibleThe authors wish to thank the funding provided by the ENPY107/18, ENPY 376/18, ENPY 304/20 projects of the Carlos III Health Institute III (ISCIII

Gender differences in adaptation to heat in Spain (1983-2018)

In Spain the average temperature has increased by 1.7 °C since pre-industrial times. There has been an increase in heat waves both in terms of frequency and intensity, with a clear impact in terms of population health. The effect of heat waves on daily mortality presents important territorial differences. Gender also affects these impacts, as a determinant that conditions social inequalities in health. There is evidence that women may be more susceptible to extreme heat than men, although there are relatively few studies that analyze differences in the vulnerability and adaptation to heat by sex. This could be related to physiological causes. On the other hand, one of the indicators used to measure vulnerability to heat in a population and its adaptation is the minimum mortality temperature (MMT) and its temporal evolution. The aim of this study was to analyze the values of MMT in men and women and its temporal evolution during the 1983-2018 period in Spain's provinces. An ecological, longitudinal retrospective study was carried out of time series data, based on maximum daily temperature and daily mortality data corresponding to the study period. Using cubic and quadratic fits between daily mortality rates and the temperature, the minimum values of these functions were determined, which allowed for determining MMT values. Furthermore, we used an improved methodology that provided for the estimation of missing MMT values when polynomial fits were inexistent. This analysis was carried out for each year. Later, based on the annual values of MMT, a linear fit was carried out to determine the rate of evolution of MMT for men and for women at the province level. Average MMT for all of Spain's provinces was 29.4 °C in the case of men and 28.7 °C in the case of women. The MMT for men was greater than that of women in 86 percent of the total provinces analyzed, which indicates greater vulnerability among women. In terms of the rate of variation in MMT during the period analyzed, that of men was 0.39 °C/decade, compared to 0.53 °C/decade for women, indicating greater adaptation to heat among women, compared to men. The differences found between men and women were statistically significant. At the province level, the results show great heterogeneity. Studies carried out at the local level are needed to provide knowledge about those factors that can explain these differences at the province level, and to allow for incorporating a gender perspective in the implementation of measures for adaptation to high temperatures.The authors wish to thank the funding provided by the ENPY 304/20, ENPY 376/18 and ENPY 107/18 projects of the Carlos III Health Institute III (ISCIII). They also wish to thank the UNED for funding this publication in open access.S

The peculiar chemical abundance of the transitional millisecond pulsar PSR J1023+0038-Li enhancement

Using high-resolution optical spectroscopy we determine the chemical abundance of the secondary star in the binary millisecond pulsar PSR J1023+0038. We measure a metallicity of [Fe/H] = 0.48 ± 0.04 which is higher than the Solar value and in general find that the element abundances are different compared to the secondary stars in X-ray binaries and stars in the solar neighbourhood of similar Fe content. Our results suggest that the pulsar was formed in a supernova explosion. We find that supernova models, where matter that has been processed in the supernova is captured by the secondary star leading to abundance anomalies, qualitatively agree with the observations. We measure Li abundance of A(Li) = 3.66 ± 0.20, which is anomalously high compared to the Li abundance of stars with the same effective temperature, irrespective of the age of the system. Furthermore, the Li abundance in PSR J1023+0038 is higher than the Cosmic value and what is observed in young Population I stars and so provides unambiguous evidence for fresh Li production. The most likely explanation is the interaction of high-energy gamma-rays or relativistic protons from the pulsar wind or intrabinary shock with the CNO nuclei in the secondary star’s atmosphere via spallation which leads to substantial Li enrichment in the secondary star’s atmosphere

Sensitivity to anti-Fas is independent of increased cathepsin D activity and adrenodoxin reductase expression occurring in NOS-3 overexpressing HepG2 cells

Stable overexpression of endothelial nitric oxide synthase (NOS-3) in HepG2 cells (4TO-NOS) leads to increased nitro-oxidative stress and upregulation of the cell death mediators p53 and Fas. Thus, NOS-3 overexpression has been suggested as a useful antiproliferative mechanism in hepatocarcinoma cells. We aimed to identify the underlying mechanism of cell death induced by NOS-3 overexpression at basal conditions and with anti-Fas treatment. The intracellular localization of NOS-3, the nitro-oxidative stress and the mitochondrial activity were analysed. In addition, the protein expression profile in 4TO-NOS was screened for differentially expressed proteins potentially involved in the induction of apoptosis. NOS-3 localization in the mitochondrial outer membrane was not associated with changes in the respiratory cellular capacity, but was related to the mitochondrial biogenesis increase and with a higher protein expression of mitochondrial complex IV. Nitro-oxidative stress and cell death in NOS-3 overexpressing cells occurred with the expression increase of pro-apoptotic genes and a higher expression/activity of the enzymes adrenodoxin reductase mitochondrial (AR) and cathepsin D (CatD). CatD overexpression in 4TO-NOS was related to the apoptosis induction independently of its catalytic activity. In addition, CatD activity inhibition by pepstatin A was not effective in blocking apoptosis induced by anti-Fas. In summary, NOS-3 overexpression resulted in an increased sensitivity to anti-Fas induced cell death, independently of AR expression and CatD activityThis study was supported by the Instituto de Salud Carlos III (FIS 09/00185). G. Ferrín was supported by the Networked Biomedical Research Center Hepatic and Digestive Diseases (CIBEREHD

Murine femur micro-computed tomography and biomechanical datasets for an ovariectomy-induced osteoporosis model

The development of new effective and safer therapies for osteoporosis, in addition to improved diagnostic and prevention strategies, represents a serious need in the scientific community. Micro-CT image-based analyses in association with biomechanical testing have become pivotal tools in identifying osteoporosis in animal models by assessment of bone microarchitecture and resistance, as well as bone strength. Here, we describe a dataset of micro-CT scans and reconstructions of 15 whole femurs and biomechanical tests on contralateral femurs from C57BL/6JOlaHsd ovariectomized (OVX), resembling human post-menopausal osteoporosis, and sham operated (sham) female mice. Data provided for each mouse include: the acquisition images (.tiff), the reconstructed images (.bmp) and an.xls file containing the maximum attenuations for each reconstructed image. Biomechanical data include an.xls file with the recorded load-displacement, a movie with the filmed test and an.xls file collecting all biomechanical results.This study was funded by Basque Country government under the ELKARTEK program No. kk-2018/00031/BC and No. kk-2019/00093/BC

Twitter as a Tool for Teaching and Communicating Microbiology: The #microMOOCSEM Initiative

Online social networks are increasingly used by the population on a daily basis. They are considered a powerful tool for science communication and their potential as educational tools is emerging. However, their usefulness in academic practice is still a matter of debate. Here, we present the results of our pioneering experience teaching a full Basic Microbiology course via Twitter (#microMOOCSEM), consisting of 28 lessons of 40-45 minutes duration each, at a tweet per minute rate during 10 weeks. Lessons were prepared by 30 different lecturers, covering most basic areas in Microbiology and some monographic topics of general interest (malaria, HIV, tuberculosis, etc.). Data analysis on the impact and acceptance of the course were largely affirmative, promoting a 330% enhancement in the followers and a >350-fold increase of the number of visits per month to the Twitter account of the host institution, the Spanish Society for Microbiology. Almost one third of the course followers were located overseas. Our study indicates that Massive Online Open Courses (MOOC) via Twitter are highly dynamic, interactive, and accessible to great audiences, providing a valuable tool for social learning and communicating science. This strategy attracts the interest of students towards particular topics in the field, efficiently complementing customary academic activities, especially in multidisciplinary areas like Microbiology.Versión del edito

Measurement of the t t-bar production cross section in the dilepton channel in pp collisions at sqrt(s) = 7 TeV

The t t-bar production cross section (sigma[t t-bar]) is measured in proton-proton collisions at sqrt(s) = 7 TeV in data collected by the CMS experiment, corresponding to an integrated luminosity of 2.3 inverse femtobarns. The measurement is performed in events with two leptons (electrons or muons) in the final state, at least two jets identified as jets originating from b quarks, and the presence of an imbalance in transverse momentum. The measured value of sigma[t t-bar] for a top-quark mass of 172.5 GeV is 161.9 +/- 2.5 (stat.) +5.1/-5.0 (syst.) +/- 3.6(lumi.) pb, consistent with the prediction of the standard model.Comment: Replaced with published version. Included journal reference and DO