Social Framework Analysis Through Oedipus Complex in Lawrence’s Sons & Lovers

Sons and Lovers has always been posing problems for the teachers, during classroom critical discussions on the novel on account of its undue association with Freud’s concept of Oedipus complex and his focus on sexual instincts. Readers are forcibly led to the conclusion that there is convincing evidence of unconscious sexual attraction between Gertrude (the mother) and William and Paul (her sons) throughout the novel. The novel basically focuses on the individual’s mental and behavioral struggle to locate his own place in the social setup in pursuit of his dignity. An open-minded close examination of the novel makes one realize that it hardly deserves to be labeled as an illustration of Freud’s theory of Oedipus complex. Paul’s struggle for recognition and acceptance in the society is the central theme of the novel which puts him in trying conditions and relations and leaves him struggling to adjust his place in quest of self-esteem and recognition. The more he tries to get satisfaction, the more he becomes disillusioned. Consequently, dissatisfaction leads him to interact with different people and he experiences mal-adjustment. The object of this paper is to analyze Paul’s relations with other main characters in the novel in order to diagnose the cause of his discontentment in his relations with them. An attempt has been made to view his predicament in the light of psychological and social cross-currents that pervade his behavior through-out the novel Paul finds intellectual harmony with Miriam as her feelings match with those of his. Like Paul, she also wants to achieve something great. A cleavage occurs in their relationship when Miriam shows her reluctance to fulfill his carnal desires, as being unethical and contrary to her spiritual ideals. Paul’s sensual desires are fulfilled by Clara, a woman promising physical satisfaction but devoid of aesthetic sensibilities which again compel him to turn to some new destinations. A thorough analysis of the novel Sons and Lovers clearly reveals that there is very little influence of Oedipus complex while there is a strong chemical bond and natural affection between the mother and son which is a natural human instinct. Paul finds solace in the company of Gertrude simply on account of natural reasons and not for any amoral urge like Oedipus complex

Global mRNA decay analysis at single nucleotide resolution reveals segmental and positional degradation patterns in a Gram-positive bacterium

Background Recent years have shown a marked increase in the use of next-generation sequencing technologies for quantification of gene expression (RNA sequencing, RNA-Seq). The expression level of a gene is a function of both its rate of transcription and RNA decay, and the influence of mRNA decay rates on gene expression in genome-wide studies of Gram-positive bacteria is under-investigated. Results In this work, we employed RNA-Seq in a genome-wide determination of mRNA half-lives in the Gram-positive bacterium Bacillus cereus. By utilizing a newly developed normalization protocol, RNA-Seq was used successfully to determine global mRNA decay rates at the single nucleotide level. The analysis revealed positional degradation patterns, with mRNAs being degraded from both ends of the molecule, indicating that both 5' to 3' and 3' to 5' directions of RNA decay are present in B. cereus. Other operons showed segmental degradation patterns where specific ORFs within polycistrons were degraded at variable rates, underlining the importance of RNA processing in gene regulation. We determined the half-lives for more than 2,700 ORFs in B. cereus ATCC 10987, ranging from less than one minute to more than fifteen minutes, and showed that mRNA decay rate correlates globally with mRNA expression level, GC content, and functional class of the ORF. Conclusions To our knowledge, this study presents the first global analysis of mRNA decay in a bacterium at single nucleotide resolution. We provide a proof of principle for using RNA-Seq in bacterial mRNA decay analysis, revealing RNA processing patterns at the single nucleotide level

A meta-analysis of photocatalytic performance and efficiency of bismuth oxide (BiO2_x)

Semiconductor-based photocatalysis is a promising technology for the future of solar fuel production. However, the solar energy conversion efficiency is not much studied. Hence it is important to create innovative semiconductor photocatalysts for environmental rehabilitation and energy production. Recently, bismuth-based oxide (BiO2-x) has seen attention due to its narrow bandgap, high number of defect sites and its unique layered structure. In this study, we discussed photocatalytic performance of the BiO2-x catalyst and its derived emergent forms through meta-analysis of data published between 2015 and 2020, allowing to explore the best available technologies prevalent for industrial applications. Various studies including pure BiO2-x, doped BiO2-x and BiO2-x based heterogeneous nanocomposites were selected through PRISMA guidelines for critical discussion. This study has been conducted with the aim of narrowing out various techniques of preparation of BiO2-x related photocatalysts, thus allowing to select the most efficient technique. The qualitative review and meta-analysis of 17 out of 21019 studies revealed that, BiO2-x despite being an excellent photocatalyst in the UV–Vis-INR range, still underperforms in comparison to its derivatives. These synthesized subsidiaries include BiO2-x based nanocomposites with a thin layer structure, doped with various elements, or coupled with other semiconductors. The current meta-analysis of data is the first of its kind and a step forward in analyzing the potential of BiO2-x and its derivatives quantitatively and extensively. It implies the future research directions that can be adopted for development of the improved techniques of preparing photocatalysts made up of BiO2-x and its derivatives

Morbidly obese parturient: Challenges for the anaesthesiologist, including managing the difficult airway in obstetrics. What is new?

The purpose of this article is to review the fundamental aspects of obesity, pregnancy and a combination of both. The scientific aim is to understand the physiological changes, pathological clinical presentations and application of technical skills and pharmacological knowledge on this unique clinical condition. The goal of this presentation is to define the difficult airway, highlight the main reasons for difficult or failed intubation and propose a practical approach to management Throughout the review, an important component is the necessity for team work between the anaesthesiologist and the obstetrician. Certain protocols are recommended to meet the anaesthetic challenges and finally concluding with “what is new?” in obstetric anaesthesia

The genome of the domesticated apple (Malus x domestica Borkh.)

We report a high-quality draft genome sequence of the domesticated apple (Malus x domestica). We show that a relatively recent (> 50 million years ago) genome-wide duplication (GWD) has resulted in the transition from nine ancestral chromosomes to 17 chromosomes in the Pyreae. Traces of older GWDs partly support the monophyly of the ancestral paleohexaploidy of eudicots. Phylogenetic reconstruction of Pyreae and the genus Malus, relative to major Rosaceae taxa, identified the progenitor of the cultivated apple as M. sieversii. Expansion of gene families reported to be involved in fruit development may explain formation of the pome, a Pyreae-specific false fruit that develops by proliferation of the basal part of the sepals, the receptacle. In apple, a subclade of MADS-box genes, normally involved in flower and fruit development, is expanded to include 15 members, as are other gene families involved in Rosaceae-specific metabolism, such as transport and assimilation of sorbitol

A map of human genome variation from population-scale sequencing

The 1000 Genomes Project aims to provide a deep characterization of human genome sequence variation as a foundation for investigating the relationship between genotype and phenotype. Here we present results of the pilot phase of the project, designed to develop and compare different strategies for genome-wide sequencing with high-throughput platforms. We undertook three projects: low-coverage whole-genome sequencing of 179 individuals from four populations; high-coverage sequencing of two mother-father-child trios; and exon-targeted sequencing of 697 individuals from seven populations. We describe the location, allele frequency and local haplotype structure of approximately 15 million single nucleotide polymorphisms, 1 million short insertions and deletions, and 20,000 structural variants, most of which were previously undescribed. We show that, because we have catalogued the vast majority of common variation, over 95% of the currently accessible variants found in any individual are present in this data set. On average, each person is found to carry approximately 250 to 300 loss-of-function variants in annotated genes and 50 to 100 variants previously implicated in inherited disorders. We demonstrate how these results can be used to inform association and functional studies. From the two trios, we directly estimate the rate of de novo germline base substitution mutations to be approximately 10−8 per base pair per generation. We explore the data with regard to signatures of natural selection, and identify a marked reduction of genetic variation in the neighbourhood of genes, due to selection at linked sites. These methods and public data will support the next phase of human genetic researc