19 research outputs found
Strategic use of serology for the diagnosis of bovine tuberculosis after intradermal skin testing
Diagnostic tests based on cell-mediated immunity are used in programmes for eradication of bovine tuberculosis (Mycobacterium bovis). Serological assays could be applied as ancillary methods to detect infected animals. Our objective was to evaluate two serological techniques: M. bovis Ab Test (IDEXX, USA) and Enferplexâą TB assay (Enfer, Ireland) in animals tested simultaneously with the single and comparative intradermal tests and the interferon-gamma assay. This work was performed at two stages. First, a preliminary panel of samples collected prior to intradermal tests from tuberculosis-free (n=60) and M. bovis-infected herds (n=78) was assayed, obtaining high specificity: 100% (M. bovis Ab Test) and 98.3% (Enferplex TB assay) but low sensitivity (detection of M. bovis infected animals): 23.9% (M. bovis Ab Test) and 32.6% (Enferplex TB assay). Subsequently, the use of serological techniques was further studied in two herds with M. bovis infection (n=77) using samples collected prior to, and 72 h and 15 days after PPD inoculation. The highest level of detection of infected animals for serology was achieved at 15 days post-intradermal tests taking advantage of the anamnestic effect: 70.4% and 85.2% in herd A, and 66.7% and 83.3% in herd B, using M. bovis Ab Test and Enferplex TB assay, respectively. Quantitative results (average values obtained with M. bovis Ab Test ELISA and degree of positivity obtained with Enferplex TB assay) were higher in animals showing lesions compatible with tuberculosis. No significant differences were observed in the number of confirmed infected animals detected with either serological technique
Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay for the Diagnosis of Bovine Tuberculosis from Milk Samples from Dairy Cows
Mycobacterium avium subsp. paratuberculosis Antibody Response, Fecal Shedding, and Antibody Cross-Reactivity to Mycobacterium bovis in M. avium subsp. paratuberculosis-Infected Cattle Herds Vaccinated against Johne's Disease
Pain Catastrophizing Thoughts Explain the Link Between Perceived Caregiver Responses and Pain Behaviors of Patients With Chronic Musculoskeletal Pain
Chronic pain severity, impact, and opioid use among patients with cancer: An analysis of biopsychosocial factors using the CHOIR learning health care system
Molecular and genetic basis of azole antifungal resistance in the opportunistic pathogenic fungus Candida albicans
PrĂ©dication en chapitre gĂ©nĂ©ral et rĂ©forme pontificale de la vita religiosa au XIIIe siĂšcle. Ă propos des sermons aux chapitres gĂ©nĂ©raux des moines noirs dâEudes de ChĂąteauroux et Jacques de Furnes
International audienceĂ partir de lâanalyse de sermons respectivement donnĂ©s par Eudes de ChĂąteauroux et Jacques de Furnes aux chapitres gĂ©nĂ©raux des moines noirs des provinces de Sens et de Reims, lâoccasion est offerte de confronter le point de vue de deux acteurs (un graduĂ© sĂ©culier ayant la faveur de la papautĂ© et un abbĂ© bĂ©nĂ©dictin au cĆur de la mĂȘlĂ©e) sur les rĂ©formes monastiques du pape GrĂ©goire IX (1235-1237) et de tenter de cerner les stratĂ©gies discursives et les mĂ©thodes de gouvernement mises en Ćuvre par la papautĂ© dans son dessein global de contrĂŽle centralisĂ© de tous les membres de lâEcclesia. Un sermon de chaque prĂ©dicateur est Ă©ditĂ© en annex