Design, synthesis, and antimicrobial screening of novel pyridyl-2-amidrazone incorporated isatin mannich bases

Isatin is an endogenous compound and reported to possess a wide range of biological activities. Numerous papers have shown that the pyridyl-2-amidrazone nucleus possesses a potent antimicrobial activity. Based on these prior observations, we postulated that a compound containing both isatin and pyridyl-2-amidrazone pharmacophores could be very effective for antimicrobial activity. Unfavorable adsorption, distribution, metabolism, and excretion (ADME) properties can in many cases lead to the clinical trials failure of potentially successful drug candidates. Their evaluation, therefore, at an earlier stage is desired. Here, we also present the predicted ADME properties of our ligands through computation. All the compounds (2a1–5) exhibited a better solubility, diffusion, Log P, molecular weight, etc., with no violations making the ligands pharmacodynamically active and better oral absorptive series. Based on the results of computational design, a series of novel pyridyl-2-amidrazone-incorporated isatin Mannich bases were synthesized and screened for their antimicrobial activities. IR, 1H-NMR, and Mass Spectroscopy data were consistent with the assigned structures. The results exhibited that all of the lead compounds showed good antimicrobial activities; noticeably, the compound 2a2 showed the best activity against Candida albicans (16 μg/ml) and compound 2a3 was found to be the most active derivative against Staphylococcus aureus and Escherichia coli at minimal inhibitory concentration values of 4 and 32 μg/ml, respectively

An evaluation of occlusal relationship and primate space in deciduous dentition in Kancheepuram District, Tamil Nadu, India

Aim: The objective of the study was to evaluate the occlusal characteristics of primary dentition in 3–5-year-old children in Kancheepuram district. Materials and Methods: A cross-sectional survey was carried out on children aged 3–5 year old in the Kancheepuram district. A total of 603 children were examined. Occlusal relationship was assessed separately on both right and left side and was classified as a flush terminal plane, mesial step, or distal step. Similarly, occlusal relationship of primary canine was examined and classified as Class I, II, or III. Primate space was measured in both the arches using digital Vernier caliper. Results: Pearson Chi-square test was used. Statistical significance was noted on both right and left side primary molar occlusion as well as in the canine occlusion across the three age groups. Primate spaces in both arches have been found to be significant across the three age groups. Mann–Whitney test between gender and occlusal relationship was found to be not significant. Conclusion: Flush terminal plane is the most common primary molar occlusion found in the present study which is statistically significant. Most of the children had primate space present in upper and lower teeth which is statistically significant

Comparison of orthopantomogram and lateral cephalogram for mandibular measurements

Aim: The aim of the present study is to clarify the possible application of orthopantomogram (OPG) for evaluating craniofacial specifications such as angular and linear measurements of the mandible by comparing with lateral cephalogram. Materials and Methods: OPG and lateral cephalogram were taken from 100 patients of age group 16–35 years from Chettinad Dental College and Research Institute. Linear measurements (body length and ramus height) and angular measurement (gonial angle) were assessed both in lateral cephalogram and OPG. Independent t-test was performed for comparison of OPG and lateral cephalogram using SPSS with a probability level of P < 0.05 considered to be statistically significant. Results: The results of the present study show that there is no statistically significant difference in ramus height and gonial angle when compared between OPG and lateral cephalogram while statistically significant difference exists for body length between OPG and lateral cephalogram. Conclusion: It may be concluded that panoramic radiography can be used to determine the gonial angle and ramus height as accurately as a lateral cephalogram. However, clinicians should be vigilant when predicting horizontal measurement from OPGs

Unraveling the Potential Role of <i>Tecomella undulata</i> in Experimental NASH

The pathophysiology of nonalcoholic steatohepatitis (NASH) is complex, owing to its diverse pathological drivers and, until recently, there were no approved drugs for this disease. Tecomella is a popular herbal medicine used to treat hepatosplenomegaly, hepatitis, and obesity. However, the potential role of Tecomella undulata in NASH has not yet been scientifically investigated. The administration of Tecomella undulata via oral gavage lowered body weight, insulin resistance, alanine transaminase (ALT), aspartate transaminase (AST), triglycerides, and total cholesterol in western diet sugar water (WDSW) fed mice but had no effect on chow diet normal water (CDNW) fed mice. Tecomella undulata improved steatosis, lobular inflammation, and hepatocyte ballooning and resolved NASH in WDSW mice. Furthermore, Tecomella undulata also alleviated the WDSW-induced Endoplasmic Reticulum stress and oxidative stress, enhanced antioxidant status, and thus reduced inflammation in the treated mice. Of note, these effects were comparable to saroglitazar, the approved drug used to treat human NASH and the positive control used in the study. Thus, our findings indicate the potential of Tecomella undulata to ameliorate WDSW-induced steatohepatitis, and these preclinical data provide a strong rationale for assessing Tecomella undulata for the treatment of NASH

Unraveling the Potential Role of Tecomella undulata in Experimental NASH

The pathophysiology of nonalcoholic steatohepatitis (NASH) is complex, owing to its diverse pathological drivers and, until recently, there were no approved drugs for this disease. Tecomella is a popular herbal medicine used to treat hepatosplenomegaly, hepatitis, and obesity. However, the potential role of Tecomella undulata in NASH has not yet been scientifically investigated. The administration of Tecomella undulata via oral gavage lowered body weight, insulin resistance, alanine transaminase (ALT), aspartate transaminase (AST), triglycerides, and total cholesterol in western diet sugar water (WDSW) fed mice but had no effect on chow diet normal water (CDNW) fed mice. Tecomella undulata improved steatosis, lobular inflammation, and hepatocyte ballooning and resolved NASH in WDSW mice. Furthermore, Tecomella undulata also alleviated the WDSW-induced Endoplasmic Reticulum stress and oxidative stress, enhanced antioxidant status, and thus reduced inflammation in the treated mice. Of note, these effects were comparable to saroglitazar, the approved drug used to treat human NASH and the positive control used in the study. Thus, our findings indicate the potential of Tecomella undulata to ameliorate WDSW-induced steatohepatitis, and these preclinical data provide a strong rationale for assessing Tecomella undulata for the treatment of NASH

Factors associated with unexplained sudden deaths among adults aged 18-45 years in India – A multicentric matched case–control study

Background & objectives: In view of anecdotal reports of sudden unexplained deaths in India's apparently healthy young adults, linking to coronavirus disease 2019 (COVID-19) infection or vaccination, we determined the factors associated with such deaths in individuals aged 18-45 years through a multicentric matched case–control study. Methods: This study was conducted through participation of 47 tertiary care hospitals across India. Cases were apparently healthy individuals aged 18-45 years without any known co-morbidity, who suddenly (<24 h of hospitalization or seen apparently healthy 24 h before death) died of unexplained causes during 1st October 2021-31st March 2023. Four controls were included per case matched for age, gender and neighborhood. We interviewed/perused records to collect data on COVID-19 vaccination/infection and post-COVID-19 conditions, family history of sudden death, smoking, recreational drug use, alcohol frequency and binge drinking and vigorous-intensity physical activity two days before death/interviews. We developed regression models considering COVID-19 vaccination ≤42 days before outcome, any vaccine received anytime and vaccine doses to compute an adjusted matched odds ratio (aOR) with 95 per cent confidence interval (CI). Results: Seven hundred twenty nine cases and 2916 controls were included in the analysis. Receipt of at least one dose of COVID-19 vaccine lowered the odds [aOR (95% CI)] for unexplained sudden death [0.58 (0.37, 0.92)], whereas past COVID-19 hospitalization [3.8 (1.36, 10.61)], family history of sudden death [2.53 (1.52, 4.21)], binge drinking 48 h before death/interview [5.29 (2.57, 10.89)], use of recreational drug/substance [2.92 (1.1, 7.71)] and performing vigorous-intensity physical activity 48 h before death/interview [3.7 (1.36, 10.05)] were positively associated. Two doses lowered the odds of unexplained sudden death [0.51 (0.28, 0.91)], whereas single dose did not. Interpretation & conclusions: COVID-19 vaccination did not increase the risk of unexplained sudden death among young adults in India. Past COVID-19 hospitalization, family history of sudden death and certain lifestyle behaviors increased the likelihood of unexplained sudden death