Esquemas de metadados utilizados por repositórios digitais científicos e de objetos educacionais : estudo preliminar

Trabalho apresentado em Conferência Luso-Brasileira sobre Acesso Aberto, 5. 2014. Coimbra, 2014Identifica esquemas de metadados utilizados para descrição de recursos em RDCs e de objetos de aprendizage

Profiles of Risky Driving Behaviors in Adolescent Drivers: A Cluster Analysis of a Representative Sample from Tuscany Region (Italy)

(1) Background: Research on patterns of risky driving behaviors (RDBs) in adolescents is scarce. This study aims to identify distinctive patterns of RDBs and to explore their characteristics in a representative sample of adolescents. (2) Methods: this is a cross-sectional study of a representative sample of Tuscany Region students aged 14–19 years (n = 2162). The prevalence of 11 RDBs was assessed and a cluster analysis was conducted to identify patterns of RDBs. ANOVA, post hoc pairwise comparisons and multivariate logistic regression models were used to characterize cluster membership. (3) Results: four distinct clusters of drivers were identified based on patterns of RDBs; in particular, two clusters—the Reckless Drivers (11.2%) and the Careless Drivers (21.5%)—showed high-risk patterns of engagement in RDBs. These high-risk clusters exhibited the weakest social bonds, the highest psychological distress, the most frequent participation in health compromising and risky behaviors, and the highest risk of a road traffic accident. (4) Conclusion: findings suggest that it is possible to identify typical profiles of RDBs in adolescents and that risky driving profiles are positively interrelated with other risky behaviors. This clustering suggests the need to develop multicomponent prevention strategies rather than addressing specific RDBs in isolation

Responsible data science: impartiality, accuracy, confidentiality and transparency of data

Introdução: no contexto Big Data, surge, como necessidade urgente, a aplicação de direitos individuais e empresariais e de normas regulatórias que resguardem a privacidade, a imparcialidade, a precisão e a transparência. Nesse cenário, a Responsible Data Science desponta como uma iniciativa que tem como base as diretrizes FACT, que correspondem à adoção de quatro princípios: imparcialidade, precisão, confidencialidade e transparência. Objetivo: abordar alternativas que podem assegurar a aplicação das diretrizes FACT. Metodologia: foi desenvolvida investigação exploratória e descritiva com abordagem qualitativa. Foram realizadas pesquisas nas bases de dados bibliográficas Web of Science, Scopus e pelo motor de busca Scholar Google com a utilização dos termos “Responsible Data Science”, “Fairness, Accuracy, Confidentiality, Transparency + Data Science”, FACT e FAT relacionados com Data Science. Resultados: a Responsible Data Science desponta como uma iniciativa que tem como base as diretrizes FACT, que correspondem à adoção dos princípios: imparcialidade, precisão, confidencialidade e transparência. Para a implementação dessas diretrizes, deve-se considerar o uso de técnicas e abordagens que estão sendo desenvolvidas pela Green Data Science. Conclusões: concluiu-se que a Green Data Science e as diretrizes FACT contribuem significativamente para a salvaguarda dos direitos individuais, não sendo necessário recorrer a medidas que impeçam o acesso e a reutilização de dados. Os desafios para implementar as diretrizes FACT requerem estudos, condição sine qua non para que as ferramentas para análise e disseminação dos dados sejam desenvolvidas ainda na fase de concepção de metodologias.Introduction: In the Big Data context, as an urgent need arises the application of individual and corporate rights and regulatory standards that safeguard privacy, impartiality, accuracy and transparency. In this scenario, Responsible Data Science emerges as an initiative based on the FACT guidelines, which correspond to the adoption of four principles: impartiality, accuracy, confidentiality and transparency. Objective: To address alternatives that can ensure the application of the FACT guidelines. Methodology: An exploratory and descriptive research with a qualitative approach was developed. Searches were performed on the Web of Science, Scopus, and Scholar Google search engines using Responsible Data Science, Fairness, Accuracy, Confidentiality, Transparency Data Science, FACT, and FAT related to Data Science. Results: Responsible Data Science emerges as an initiative based on the FACT guidelines, which correspond to the adoption of the principles: impartiality, accuracy, confidentiality and transparency. In implementing these guidelines, consideration should be given to the use of techniques and approaches being developed by Green Data Science. Conclusions: It is concluded that Green Data Science and the FACT guidelines contribute significantly to safeguarding individual rights and that no measures need to be taken to prevent access and reuse of data. Challenges for implementing the FACT guidelines require studies, sine qua non conditions for tools for data analysis and dissemination to be developed at the design stage of methodologies.Introducción: en el contexto de Big Data, como una necesidad urgente surge la aplicación de los derechos individuales y corporativos y las normas reguladoras que salvaguardan la privacidad, imparcialidad, precisión y transparencia. En este escenario, Responsible Data Science surge como una iniciativa basada en las pautas de FACT, que corresponden a la adopción de cuatro principios: imparcialidad, precisión, confidencialidad y transparencia. Objetivo: abordar alternativas que puedan garantizar la aplicación de las pautas de FACT. Metodología: se desarrolló una investigación exploratoria y descriptiva con un enfoque cualitativo. Las búsquedas se realizaron en los motores de búsqueda de Web of Science, Scopus y Scholar Google utilizando los términos "Ciencia de datos responsable", "Justicia, precisión, confidencialidad, transparencia + ciencia de datos", FACT y FAT relacionados con ciência de los datos. Resultados: Responsible Data Science surge como una iniciativa basada en los lineamientos de FACT, que corresponden a la adopción de los principios: imparcialidad, precisión, confidencialidad y transparencia. Al implementar estas pautas, se debe considerar el uso de técnicas y enfoques desarrollados por Green Data Science. Conclusiones: Se concluye que Green Data Science y las pautas FACT contribuyen significativamente a salvaguardar los derechos individuales y que no es necesario tomar medidas para evitar el acceso y la reutilización de datos. Los desafíos para implementar las pautas FACT requieren estudios, condiciones sine qua non para desarrollar herramientas para el análisis y la difusión de datos en la etapa de diseño de las metodologias

Minimal residual disease negativity by next-generation flow cytometry is associated with improved organ response in AL amyloidosis

© The Author(s) 2021.Light chain (AL) amyloidosis is caused by a small B-cell clone producing light chains that form amyloid deposits and cause organ dysfunction. Chemotherapy aims at suppressing the production of the toxic light chain (LC) and restore organ function. However, even complete hematologic response (CR), defined as negative serum and urine immunofixation and normalized free LC ratio, does not always translate into organ response. Next-generation flow (NGF) cytometry is used to detect minimal residual disease (MRD) in multiple myeloma. We evaluated MRD by NGF in 92 AL amyloidosis patients in CR. Fifty-four percent had persistent MRD (median 0.03% abnormal plasma cells). There were no differences in baseline clinical variables in patients with or without detectable MRD. Undetectable MRD was associated with higher rates of renal (90% vs 62%, p = 0.006) and cardiac response (95% vs 75%, p = 0.023). Hematologic progression was more frequent in MRD positive (0 vs 25% at 1 year, p = 0.001). Altogether, NGF can detect MRD in approximately half the AL amyloidosis patients in CR, and persistent MRD can explain persistent organ dysfunction. Thus, this study supports testing MRD in CR patients, especially if not accompanied by organ response. In case MRD persists, further treatment could be considered, carefully balancing residual organ damage, patient frailty, and possible toxicity.This study was supported by a grant from CARIPLO “Molecular mechanisms of Ig toxicity in age-related plasma cell dyscrasias no. 2015-0591”, by a grant from the Black Swan Research Initiative from the International Myeloma Foundation “Automated multidimensional flow cytometry for high-sensitive screening and to monitor response in AL amyloidosis”, by a grant from CARIPLO “Structure–function relation of amyloid: understanding the molecular bases of protein misfolding diseases to design new treatments no. 2013-0964”, by a grant from the Amyloidosis Foundation “Investigating new therapies to treat AL amyloidosis”, and by a grant from Cancer Research UK, FCAECC and AIRC under the Accelerator Award 2017 Program “Early detection and intervention: understanding the mechanisms of transformation and hidden resistance of incurable hematological malignancies”, by a grant from CARIPLO “Harnessing the plasma cell secretory capacity against systemic light chain amyloidosis” (no. 2018-0257), by a grant from the Italian Ministry of Health “Towards effective, patient-tailored anti-plasma cell therapies in AL amyloidosis: predicting drug response and overcoming drug resistance” (GR-2018-12368387). This study has also supported the Centro de Investigación Biomédica en Red—Área de Oncología—del Instituto de Salud Carlos III (CIBERONC; CB16/12/00369, CB16/12/00400, and CB16/12/00489) and the Instituto de Salud Carlos III/Subdirección General de Investigación Sanitaria (FIS No. PI13/02196). G.P. is supported in part by the Bart Barlogie Young Investigator Award from the International Myeloma Society (IMS). P.M. is supported in part by a fellowship grant form Collegio Ghislieri (Pavia). We acknowledge the study coordinator and data manager Anna Carnevale Baraglia

Multifunctional nanoassemblies target bacterial lipopolysaccharides for enhanced antimicrobial DNA delivery

The development of new therapeutic strategies against multidrug resistant Gram-negative bacteria is a major challenge for pharmaceutical research. In this respect, it is increasingly recognized that an efficient treatment for resistant bacterial infections should combine antimicrobial and anti-inflammatory effects. Here, we explore the multifunctional therapeutic potential of nanostructured self-assemblies from a cationic bolaamphiphile, which target bacterial lipopolysaccharides (LPSs) and associates with an anti-bacterial nucleic acid to form nanoplexes with therapeutic efficacy against Gram-negative bacteria. To understand the mechanistic details of these multifunctional antimicrobial-anti-inflammatory properties, we performed a fundamental study, comparing the interaction of these nanostructured therapeutics with synthetic biomimetic bacterial membranes and live bacterial cells. Combining a wide range of experimental techniques (Confocal Microscopy, Fluorescence Correlation Spectroscopy, Microfluidics, NMR, LPS binding assays), we demonstrate that the LPS targeting capacity of the bolaamphiphile self-assemblies, comparable to that exerted by Polymixin B, is a key feature of these nanoplexes and one that permits entry of therapeutic nucleic acids in Gram-negative bacteria. These findings enable a new approach to the design of efficient multifunctional therapeutics with combined antimicrobial and anti-inflammatory effects and have therefore the potential to broadly impact fundamental and applied research on self-assembled nano-sized antibacterials for antibiotic resistant infections

Antimicrobial resistance among migrants in Europe: a systematic review and meta-analysis

BACKGROUND: Rates of antimicrobial resistance (AMR) are rising globally and there is concern that increased migration is contributing to the burden of antibiotic resistance in Europe. However, the effect of migration on the burden of AMR in Europe has not yet been comprehensively examined. Therefore, we did a systematic review and meta-analysis to identify and synthesise data for AMR carriage or infection in migrants to Europe to examine differences in patterns of AMR across migrant groups and in different settings. METHODS: For this systematic review and meta-analysis, we searched MEDLINE, Embase, PubMed, and Scopus with no language restrictions from Jan 1, 2000, to Jan 18, 2017, for primary data from observational studies reporting antibacterial resistance in common bacterial pathogens among migrants to 21 European Union-15 and European Economic Area countries. To be eligible for inclusion, studies had to report data on carriage or infection with laboratory-confirmed antibiotic-resistant organisms in migrant populations. We extracted data from eligible studies and assessed quality using piloted, standardised forms. We did not examine drug resistance in tuberculosis and excluded articles solely reporting on this parameter. We also excluded articles in which migrant status was determined by ethnicity, country of birth of participants' parents, or was not defined, and articles in which data were not disaggregated by migrant status. Outcomes were carriage of or infection with antibiotic-resistant organisms. We used random-effects models to calculate the pooled prevalence of each outcome. The study protocol is registered with PROSPERO, number CRD42016043681. FINDINGS: We identified 2274 articles, of which 23 observational studies reporting on antibiotic resistance in 2319 migrants were included. The pooled prevalence of any AMR carriage or AMR infection in migrants was 25·4% (95% CI 19·1-31·8; I2 =98%), including meticillin-resistant Staphylococcus aureus (7·8%, 4·8-10·7; I2 =92%) and antibiotic-resistant Gram-negative bacteria (27·2%, 17·6-36·8; I2 =94%). The pooled prevalence of any AMR carriage or infection was higher in refugees and asylum seekers (33·0%, 18·3-47·6; I2 =98%) than in other migrant groups (6·6%, 1·8-11·3; I2 =92%). The pooled prevalence of antibiotic-resistant organisms was slightly higher in high-migrant community settings (33·1%, 11·1-55·1; I2 =96%) than in migrants in hospitals (24·3%, 16·1-32·6; I2 =98%). We did not find evidence of high rates of transmission of AMR from migrant to host populations. INTERPRETATION: Migrants are exposed to conditions favouring the emergence of drug resistance during transit and in host countries in Europe. Increased antibiotic resistance among refugees and asylum seekers and in high-migrant community settings (such as refugee camps and detention facilities) highlights the need for improved living conditions, access to health care, and initiatives to facilitate detection of and appropriate high-quality treatment for antibiotic-resistant infections during transit and in host countries. Protocols for the prevention and control of infection and for antibiotic surveillance need to be integrated in all aspects of health care, which should be accessible for all migrant groups, and should target determinants of AMR before, during, and after migration. FUNDING: UK National Institute for Health Research Imperial Biomedical Research Centre, Imperial College Healthcare Charity, the Wellcome Trust, and UK National Institute for Health Research Health Protection Research Unit in Healthcare-associated Infections and Antimictobial Resistance at Imperial College London

Covid-19 and the role of smoking: the protocol of the multicentric prospective study COSMO-IT (COvid19 and SMOking in ITaly).

The emergency caused by Covid-19 pandemic raised interest in studying lifestyles and comorbidities as important determinants of poor Covid-19 prognosis. Data on tobacco smoking, alcohol consumption and obesity are still limited, while no data are available on the role of e-cigarettes and heated tobacco products (HTP). To clarify the role of tobacco smoking and other lifestyle habits on COVID-19 severity and progression, we designed a longitudinal observational study titled COvid19 and SMOking in ITaly (COSMO-IT). About 30 Italian hospitals in North, Centre and South of Italy joined the study. Its main aims are: 1) to quantify the role of tobacco smoking and smoking cessation on the severity and progression of COVID-19 in hospitalized patients; 2) to compare smoking prevalence and severity of the disease in relation to smoking in hospitalized COVID-19 patients versus patients treated at home; 3) to quantify the association between other lifestyle factors, such as e-cigarette and HTP use, alcohol and obesity and the risk of unfavourable COVID-19 outcomes. Socio-demographic, lifestyle and medical history information will be gathered for around 3000 hospitalized and 700-1000 home-isolated, laboratory-confirmed, COVID-19 patients. Given the current absence of a vaccine against SARS-COV-2 and the lack of a specific treatment for -COVID-19, prevention strategies are of extreme importance. This project, designed to highly contribute to the international scientific debate on the role of avoidable lifestyle habits on COVID-19 severity, will provide valuable epidemiological data in order to support important recommendations to prevent COVID-19 incidence, progression and mortality