11 research outputs found
The management of desmoid tumours: A joint global consensus-based guideline approach for adult and paediatric patients
Abstract Desmoid tumor (DT; other synonymously used terms: Desmoid-type fibromatosis, aggressive fibromatosis) is a rare and locally aggressive monoclonal, fibroblastic proliferation characterised by a variable and often unpredictable clinical course. Previously surgery was the standard primary treatment modality; however, in recent years a paradigm shift towards a more conservative management has been introduced and an effort to harmonise the strategy amongst clinicians has been made. We present herein an evidence-based, joint global consensus guideline approach to the management of this disease focussing on: molecular genetics, indications for an active treatment, and available systemic therapeutic options. This paper follows a one-day consensus meeting held in Milan, Italy, in June 2018 under the auspices of the European Reference Network for rare solid adult cancers, EURACAN, the European Organisation for Research and Treatment of Cancer (EORTC) Soft Tissue and Bone Sarcoma Group (STBSG) as well as Sarcoma Patients EuroNet (SPAEN) and The Desmoid tumour Research Foundation (DTRF). The meeting brought together over 50 adult and pediatric sarcoma experts from different disciplines, patients and patient advocates from Europe, North America and Japan
Unique and overlapping GLI1 and GLI2 transcriptional targets in neoplastic chondrocytes.
Excessive Hedgehog (Hh) signaling in chondrocytes is sufficient to cause formation of enchondroma-like lesions which can progress to chondrosarcoma. To elucidate potential underlying mechanisms, we identified GLI1 and GLI2 target genes in human chondrosarcoma. Using chromatin immunoprecipitation (ChIP) sequencing and microarray data, in silico analyses were conducted to identify and characterize unique and overlapping GLI1 and GLI2 binding regions in neoplastic chondrocytes. After overlaying microarray data from human chondrosarcoma, 204 upregulated and 106 downregulated genes were identified as Hh-responsive Gli binding targets. After overlaying published Gli ChIP-on-chip data from mouse, 48 genes were identified as potential direct downstream targets of Hedgehog signaling with shared GLI binding regions in evolutionarily conserved DNA elements. Among these was BMP2, pointing to potential cross-talk between TGF beta signaling and Hh signaling. Our identification of potential target genes that are unique and common to GLI1 and GLI2 in neoplastic chondrocytes contributes to elucidating potential pathways through which Hh signaling impacts cartilage tumor biology
Diagnosis of limb-girdle muscular dystrophy 2A by immunohistochemical techniques
The Western blot technique is currently the standard detection method for suspected limb girdle muscular dystrophy (LGMD) 2A (calpainopathy). This is the first report in the English literature of the successful application of immunohistochemical techniques to support a diagnosis of LGMD 2A. This approach is straightforward and appears to be reasonably specific. We propose that immunohistochemical methods should be re-evaluated for the screening of undiagnosed patients with suspected LGMD 2A
Phenotype Determines Nanoparticle Uptake by Human Macrophages from Liver and Blood
A significant challenge to delivering
therapeutic doses of nanoparticles to targeted disease sites is the
fact that most nanoparticles become trapped in the liver. Liver-resident
macrophages, or Kupffer cells, are key cells in the hepatic sequestration
of nanoparticles. However, the precise role that the macrophage phenotype
plays in nanoparticle uptake is unknown. Here, we show that the human
macrophage phenotype modulates hard nanoparticle uptake. Using gold
nanoparticles, we examined uptake by human monocyte-derived macrophages
that had been driven to a “regulatory” M2 phenotype
or an “inflammatory” M1 phenotype and found that M2-type
macrophages preferentially take up nanoparticles, with a clear hierarchy
among the subtypes (M2c > M2 > M2a > M2b > M1). We also
found that stimuli such as LPS/IFN-γ rather than with more “regulatory”
stimuli such as TGF-β/IL-10 reduce per cell macrophage nanoparticle
uptake by an average of 40%. Primary human Kupffer cells were found
to display heterogeneous expression of M1 and M2 markers, and Kupffer
cells expressing higher levels of M2 markers (CD163) take up significantly
more nanoparticles than Kupffer cells expressing lower levels of surface
CD163. Our results demonstrate that hepatic inflammatory microenvironments
should be considered when studying liver sequestration of nanoparticles,
and that modifying the hepatic microenvironment might offer a tool
for enhancing or decreasing this sequestration. Our findings also
suggest that models examining the nanoparticle/macrophage interaction
should include studies with primary tissue macrophages
Forearm reconstruction with bone allograft following tumor excision: A series of 10 patients with a mean follow-up of 10 years
Fibrous Dysplasia
Recognition of fibrous dysplasia (FD) as a distinct skeletal disease is commonly attributed to the description of an osteitis fibrosa disseminata occurring in conjunction with various endocrinopathies and skin pigmentation. The bone that is turned over more rapidly in FD is abnormal, qualitatively and quantitatively, in many other critical features ranging from primary modeling to chemical composition, which more directly translate into clinically adverse effects. FD is a disease of excess; abnormal, and imperfect bone growth. The disease produces excess bone growth by causing a localized increase in bone tissue within bone (or local bone mass). The disease causes abnormal bone growth because bone formation does not adhere to the architectural design of the affected, growing bone segments. The territorial definition of cortical bone, cancellous bone, and marrow space is lost, and bone is formed with haphazard trabecular architecture and an irregular internal structure and is mechanically unsound. The disease causes imperfect bone growth because the matrix deposited has an abnormal chemical composition, an abnormal "tricotage," and an abnormal mineral content. © 2012 Elsevier Inc. All rights reserved