The fishes of ascension Island, central Atlantic Ocean - new records and an annotated checklist

A checklist of the fishes of Ascension Island is presented. The species Rhincodon typus, Alopias superciliosus, Isurus oxyrinchus, Carcharhinus obscurus, Galeocerdo cuvier, Sphyrna lewini, Hexanchus griseus, Manta birostris, Gymnothorax vicinus, Hippocampus sp., Epinephelus itajara, Cookeolus japonicus, Apogon pseudomaculatus, Phaeoptyx pigmentaria, Remora albescens, Caranx bartholomaei, Carangoides ruber, Decapterus tabl, Seriola dumerili, Thalassoma sanctaehelenae, Cryptotomus sp., Ruvettus pretiosus, Acanthocybium solandri, Auxis rochei, Auxis thazard, Euthynnus alletteratus, Katsuwonus pelamis, Thunnus alalunga, Thunnus obesus, Xiphias gladius, Istiophorus platypterus, Kajikia albida, Makaira nigricans, Tetrapturus pfluegeri, Hyperoglyphe perciformis, Schedophilus sp., Cantherhines macrocerus, Sphoeroides pachygaster and Diodon eydouxii are recorded for the first time from Ascension Island. We have recognized two previous records as identification errors and indicate 11 other records as doubtful. Including the 40 new records, we now list 173 fish species from Ascension Island, of which 133 might be considered 'coastal fish species'. Eleven of these (8.3%) appear to be endemic to the island and a further 16 species (12%) appear to be shared endemics with St Helena Island.Darwin Initiative [EIDCF012]info:eu-repo/semantics/publishedVersio

Quantitative cross-species extrapolation between humans and fish: The case of the anti-depressant fluoxetine

This article has been made available through the Brunel Open Access Publishing Fund.Fish are an important model for the pharmacological and toxicological characterization of human pharmaceuticals in drug discovery, drug safety assessment and environmental toxicology. However, do fish respond to pharmaceuticals as humans do? To address this question, we provide a novel quantitative cross-species extrapolation approach (qCSE) based on the hypothesis that similar plasma concentrations of pharmaceuticals cause comparable target-mediated effects in both humans and fish at similar level of biological organization (Read-Across Hypothesis). To validate this hypothesis, the behavioural effects of the anti-depressant drug fluoxetine on the fish model fathead minnow (Pimephales promelas) were used as test case. Fish were exposed for 28 days to a range of measured water concentrations of fluoxetine (0.1, 1.0, 8.0, 16, 32, 64 μg/L) to produce plasma concentrations below, equal and above the range of Human Therapeutic Plasma Concentrations (HTPCs). Fluoxetine and its metabolite, norfluoxetine, were quantified in the plasma of individual fish and linked to behavioural anxiety-related endpoints. The minimum drug plasma concentrations that elicited anxiolytic responses in fish were above the upper value of the HTPC range, whereas no effects were observed at plasma concentrations below the HTPCs. In vivo metabolism of fluoxetine in humans and fish was similar, and displayed bi-phasic concentration-dependent kinetics driven by the auto-inhibitory dynamics and saturation of the enzymes that convert fluoxetine into norfluoxetine. The sensitivity of fish to fluoxetine was not so dissimilar from that of patients affected by general anxiety disorders. These results represent the first direct evidence of measured internal dose response effect of a pharmaceutical in fish, hence validating the Read-Across hypothesis applied to fluoxetine. Overall, this study demonstrates that the qCSE approach, anchored to internal drug concentrations, is a powerful tool to guide the assessment of the sensitivity of fish to pharmaceuticals, and strengthens the translational power of the cross-species extrapolation

The central energy source of 70micron-selected galaxies: Starburst or AGN?

We present the first AGN census in a sample of 61 galaxies selected at 70microns, a wavelength which should strongly favour the detection of star-forming systems. For the purpose of this study we take advantage of deep Chandra X-ray and Spitzer infrared (3.6-160micron) data, as well as optical spectroscopy and photometry from the Deep Extragalactic Evolutionary Probe 2 (DEEP2) survey for the Extended Groth Strip (EGS) field. We investigate spectral line diagnostics ([OIII]/Hbeta and [NeIII]/[OII] ratios, Hdelta Balmer absorption line equivalent widths and the strength of the 4000Ang break), X-ray luminosities and spectral energy distributions (SEDs). We find that the 70micron sources are undergoing starburst episodes and are therefore characterised by a predominance of young stars. In addition, 13 per cent of the sources show AGN signatures and hence potentially host an AGN. When the sample is split into starbursts (SBs, 10^10<L_IR<10^11 L_solar), Luminous InfraRed Galaxies (LIRGs, 10^11<L_IR<10^12 L_solar) and UltraLuminous InfraRed Galaxies (ULIRGs,10^12<L_IR<10^13 L_solar), the AGN fraction becomes 0, 11 and 23 per cent respectively, showing an increase with total infrared luminosity. However, by examining the sources' panchromatic SEDs, we conclude that although the AGN is energetically important in 1 out of 61 objects, all 70micron-selected galaxies are primarily powered by star-formation.Comment: 20 pages, 14 figures, accepted for publication in MNRA

Searching for Compton-thick active galactic nuclei at z~0.1

Using a suite of X-ray, mid-IR and optical active galactic nuclei (AGN) luminosity indicators, we search for Compton-thick (CT) AGNs with intrinsic L_X>10^42erg/s at z~0.03-0.2, a region of parameter space which is currently poorly constrained by deep narrow-field and high-energy (E>10keV) all-sky X-ray surveys. We have used the widest XMM-Newton survey (the serendipitous source catalogue) to select a representative sub-sample (14; ~10%) of the 147 X-ray undetected candidate CT AGNs in the Sloan Digital Sky Survey (SDSS) with f_X/f_[OIII]<1; the 147 sources account for ~50% of the overall Type-2 AGN population in the SDSS-XMM overlap region. We use mid-IR spectral decomposition analyses and emission-line diagnostics, determined from pointed Spitzer-IRS spectroscopic observations of these candidate CT AGNs, to estimate the intrinsic AGN emission (predicted L_X,2-10keV (0.2-30)x10^42erg/s). On the basis of the optical [OIII], mid-IR [OIV] and 6um AGN continuum luminosities we conservatively find that the X-ray emission in at least 6/14 (>43%) of our sample appear to be obscured by CT material with N_H>1.5x10^24cm^-2. Under the reasonable assumption that our 14 AGNs are representative of the overall X-ray undetected AGN population in the SDSS-XMM parent sample, we find that >20% of the optical Type-2 AGN population are likely to be obscured by CT material. This implies a space-density of log(Phi) >-4.9Mpc^-3 for CT AGNs with L_X>10^42erg/s at z~0.1, which we suggest may be consistent with that predicted by X-ray background synthesis models. Furthermore, using the 6um continuum luminosity to infer the intrinsic AGN luminosity and the stellar velocity dispersion to estimate M_BH, we find that the most conservatively identified CT AGNs in this sample may harbour some of the most rapidly growing black holes (median M_BH~3x10^7M_o) in the nearby Universe, with a median Eddington ratio of ~0.2.Comment: 16 pages, 2 tables, 6 figures. Accepted for publication in MNRA

Obscured and powerful AGN and starburst activities at z~3.5

We report the discovery of two sources at z=3.867 and z=3.427 that exhibit powerful starburst and AGN activities. They benefit from data from radio to X rays from the CFHTLS-D1/SWIRE/XMDS surveys. Follow-up optical and near-infrared spectroscopy, and millimeter IRAM/MAMBO observations are also available. We performed an analysis of their spectral energy distributions to understand the origin of their emission and constrain their luminosities. A comparison with other composite systems at similar redshifts from the literature is also presented. The AGN and starburst bolometric luminosities are ~10^13 Lsun. The AGN emission dominates at X ray, optical, mid-infrared wavelengths, and probably in the radio. The starburst emission dominates in the far-infrared. The estimated star formation rates range from 500 to 3000Msun/yr. The AGN near-infrared and X ray emissions are heavily obscured in both sources with an estimated dust extinction Av>4, and Compton-thick gas column densities. The two sources are the most obscured and most luminous AGNs detected at millimeter wavelengths currently known. The sources presented in this work are heavily obscured QSOs, but their properties are not fully explained by the standard AGN unification model. In one source, the ultraviolet and optical spectra suggest the presence of outflowing gas and shocks, and both sources show emission from hot dust, most likely in the vicinity of the nucleus. Evidence of moderate AGN-driven radio activity is found in both sources. The two sources lie on the local M_BH-M_bulge relation. To remain on this relation, their star formation rate has to decrease. Our results support evolutionary models that invoke radio feedback as star formation quenching mechanism, and suggest that such a mechanism might play a major role also in powerful AGNs.Comment: Accepted for publication in Astronomy & Astrophysics (12 pages; 6 figures); replaced version includes minor language editing and revised reference

Loss of Arp2/3 induces an NF-κB–dependent, nonautonomous effect on chemotactic signaling

A decrease in Arp2/3 levels results in an NF-κB–dependent increase in the expression of several secreted factors, resulting in nonautonomous effects on chemotaxis.Arp2/3-branched actin is critical for cytoskeletal dynamics and cell migration. However, perturbations and diseases affecting this network have phenotypes that cannot be fully explained by cell-autonomous effects. In this paper, we report nonautonomous effects of Arp2/3 depletion. We show that, upon Arp2/3 depletion, the expression of numerous genes encoding secreted factors, including chemokines, growth factors, and matrix metalloproteases, was increased, a signature resembling the senescence-associated secretory phenotype. These factors affected epidermal growth factor chemotaxis in a nonautonomous way, resolving the recent contradictions about the role of Arp2/3 in chemotaxis. We demonstrate that these genes were activated by nuclear factor κB via a CCM2–MEKK3 pathway that has been implicated in hyperosmotic stress signaling. Consistent with this, Arp2/3-depleted cells showed misregulation of volume control and reduced actin in the submembranous cortex. The defects in osmotic signaling in the Arp2/3-depleted cells can be rescued by hypoosmotic treatment. Thus, perturbations of Arp2/3 have nonautonomous effects that should be considered when evaluating experimental manipulations and diseases affecting the Arp2/3-actin cytoskeleton

Narrow-line AGN in the ISO-2MASS Survey

Context: A long-standing challenge of observational AGN research is to find type 2 quasars, the luminous analogues of Seyfert-2 galaxies. Aims: We search for luminous narrow-line type 2 AGN, characterise their properties, and compare them with broad-line type 1 AGN. Methods: Combining the ISOCAM parallel survey at 6.7 mu with 2MASS, we have selected AGN via near-mid-infrared colours caused by the hot nuclear dust emission. We performed spectroscopy in the optical and, for a subset of the sample, also in the mid-infrared with Spitzer. Results: We find nine type 2 AGN at redshift 0.1<z<0.5, three of them have even quasar-like [OIII] luminosities. At the given redshift and luminosity range the number of type 2 AGN is at least as high as that of type 1s. At z>0.5 we did not find type 2 AGN, probably because the hottest dust emission, still covered by the NIR filters, is obscured. The optical spectra of the type 2 host galaxies show young and old stellar populations. Only one object is an ultraluminous infrared galaxy with starburst. The 5-38 mu spectra of the two type 2 sources observed show a strong continuum with PAH emission in one case and silicate absorption in the other case. Conclusions: The near-mid-infrared selection is a successful strategy to find luminous type 2 AGN at low z. The objects exhibit a large range of properties so that it is difficult to infer details by means of popular SED fitting with simple average templates.Comment: Accepted for publication in A&

Updating test-day milk yield factors for use in genetic evaluations and dairy production systems: a comprehensive review

Various methods have been proposed to estimate daily yield from partial yields, primarily to deal with unequal milking intervals. This paper offers an exhaustive review of daily milk yields, the foundation of lactation records. Seminal advancements in the late 20th century concentrated on two main adjustment metrics: additive additive correction factors (ACF) and multiplicative correction factors (MCF). An ACF model provides additive adjustments to two times AM or PM milk yield, which then becomes the estimated daily yields, whereas an MCF is a ratio of daily yield to the yield from a single milking. Recent studies highlight the potential of alternative approaches, such as exponential regression and other nonlinear models. Biologically, milk secretion rates are not linear throughout the entire milking interval, influenced by the internal mammary gland pressure. Consequently, nonlinear models are appealing for estimating daily milk yields as well. MCFs and ACFs are typically determined for discrete milking interval classes. Nonetheless, large discrete intervals can introduce systematic biases. A universal solution for deriving continuous correction factors has been proposed, ensuring reduced bias and enhanced daily milk yield estimation accuracy. When leveraging test-day milk yields for genetic evaluations in dairy cattle, two predominant statistical models are employed: lactation and test-day yield models. A lactation model capitalizes on the high heritability of total lactation yields, aligning closely with dairy producers’ needs because the total amount of milk production in a lactation directly determines farm revenue. However, a lactation yield model without harnessing all test-day records may ignore vital data about the shapes of lactation curves needed for informed breeding decisions. In contrast, a test-day model emphasizes individual test-day data, accommodating various intervals and recording plans and allowing the estimation of environmental effects on specific test days. In the United States, the patenting of test-day models in 1993 used to restrict the use of test-day models to regional and unofficial evaluations by the patent holders. Estimated test-day milk yields have been used as if they were accurate depictions of actual milk yields, neglecting possible estimation errors. Its potential consequences on subsequent genetic evaluations have not been sufficiently addressed. Moving forward, there are still numerous questions and challenges in this domain

Nuclear Factor (NF) κB polymorphism is associated with heart function in patients with heart failure

<p>Abstract</p> <p>Background</p> <p>Cardiac remodeling is generally an adverse sign and is associated with heart failure (HF) progression. NFkB, an important transcription factor involved in many cell survival pathways, has been implicated in the remodeling process, but its role in the heart is still controversial. Recently, a promoter polymorphism associated with a lesser activation of the <it>NFKB1 </it>gene was also associated with Dilated Cardiomyopathy. The purpose of this study was to evaluate the association of this polymorphism with clinical and functional characteristics of heart failure patients of different etiologies.</p> <p>Methods</p> <p>A total of 493 patients with HF and 916 individuals from a cohort of individuals from the general population were investigated. The <it>NFKB1 </it>-94 insertion/deletion ATTG polymorphism was genotyped by High Resolution Melt discrimination. Allele and genotype frequencies were compared between groups. In addition, frequencies or mean values of different phenotypes associated with cardiovascular disease were compared between genotype groups. Finally, patients were prospectively followed-up for death incidence and genotypes for the polymorphism were compared regarding disease onset and mortality incidence in HF patients.</p> <p>Results</p> <p>We did not find differences in genotype and allelic frequencies between cases and controls. Interestingly, we found an association between the ATTG₁/ATTG₁genotype with right ventricle diameter (<it>P </it>= 0.001), left ventricle diastolic diameter (P = 0.04), and ejection fraction (EF) (P = 0.016), being the genotype ATTG₁/ATTG₁more frequent in patients with EF lower than 50% (<it>P </it>= 0.01). Finally, we observed a significantly earlier disease onset in ATTG1/ATTG₁carriers.</p> <p>Conclusion</p> <p>There is no genotype or allelic association between the studied polymorphism and the occurrence of HF in the tested population. However, our data suggest that a diminished activation of <it>NFKB1</it>, previously associated with the ATTG₁/ATTG₁genotype, may act modulating on the onset of disease and, once the individual has HF, the genotype may modulate disease severity by increasing cardiac remodeling and function deterioration.</p

Impacts of High-Protein Oral Nutritional Supplements Among Malnourished Men and Women with Sarcopenia: A Multicenter, Randomized, Double-Blinded, Controlled Trial.

BACKGROUND: Recent evidence suggests that nutritional interventions may improve muscle outcomes in malnutrition and sarcopenia. OBJECTIVES: We evaluated the effects of 2 high-quality oral nutritional supplements (ONS) differing in amount and type of key nutrients in older adult men and women. DESIGN: A multicenter, randomized, double-blinded, controlled clinical trial. PARTICIPANTS: Malnourished and sarcopenic men and women, 65 years and older (n = 330). INTERVENTION: A 24-week intervention period with 2 energy-rich (330 kcal) ONS treatment groups: Control ONS (CONS, 14 g protein; 147 IU vitamin D3) versus Experimental ONS (EONS, 20 g protein; 499 IU vitamin D3; 1.5 g CaHMB) taken twice daily. Both ONS also contained other vitamins, minerals, and nutrients in varying amounts. MEASUREMENTS: Isokinetic peak torque (PT, Nm) leg strength, grip strength (kg), and gait speed (m·s-1) were assessed at baseline and 12 and 24 weeks. Left and right leg muscle mass (LMM, kg) were assessed by dual-energy x-ray absorptiometry (DXA). Muscle quality (MQ) was leg strength expressed relative to the tested LMM (Nm·kg-1). Subgroup analyses were performed: severe sarcopenia (low skeletal mass index, low grip strength [ CONS, P = .032) in those with normal grip strength. There were no treatment differences based on sarcopenic severity for either grip strength or gait speed. CONCLUSION: ONS improved strength outcomes in malnourished older adults with sarcopenia. In those with mild-moderate sarcopenia, but not severe sarcopenia, consumption of the EONS improved leg muscle strength and quality compared with the standard CONS