Composição química e atividade antimicrobiana do óleo essencial de folhas e flores de Aloysia gratissima

Volatile oils from leaves and flowers of Aloysia gratissima were investigated for their chemical composition and antimicrobial activity against the bacteria Bacilus subtilis, Staphylococcus aureus, Salmonella choleraesuis, Pseudomonas aeruginosa, Streptococcus pneumoniae and the Candida albicans yeast. The minimum inhibitory concentrations (MIC) of the oils were determined by the micro-dilution method, while the chemical composition was determined by GC-MS (gas chromatography mass spectrometry). The fresh leaves and inflorescence were subjected to hydrodistillation for 120 min using a Clevenger-type apparatus, and the essential oil was tested against microorganisms. High concentrations of sesquiterpenes were observed for the inflorescence, and monoterpenes were observed for the leaves. The main compounds of the inflorescence essential oil were E-caryophyllene, germacrene B, guaiol and bulnesol, while in the leaves the main compounds were trans-pinocamphone, trans-pinocarveyl acetate, and guaiol. The essential oil from the leaves showed an effect against P. aeruginosa and S. pneumonia, and the essential oil of the inflorescence showed an effect against P. aeruginosa, S. pneumonia, and Candida albicans.O óleo essencial de folhas e de flores de Aloysia gratissima foi avaliado quanto à composição química e ação antimicrobiana contra as bactérias Bacilus subtilis, Staphylococcus aureus, Salmonella choleraesuis, Pseudomonas aeruginosa, Streptococcus pneumoniae, e a levedura Candida albicans. A concentração mínima inibitória (MIC) dos óleos essenciais foi determinada pelo método da microdiluição e a composição química determinada por CG-EM (Cromatografia Gasosa acoplada a Espectrômetro de Massas). Folhas e inflorescências frescas foram hidrodestiladas por 120 minutos em aparelho Clevenger sendo o óleo essencial testado contra microorganismos. Para as flores foi observada maior concentração de sesquiterpenos, enquanto que as folhas apresentaram maior concentração de monoterpenos. Os principais constituintes do óleo essencial da flor foram: E-cariofileno, germacreno B, guaiol e bulnesol; e das folhas foram: trans-pinocamfona, acetato de trans-pinocarveol e guaiol. O óleo essencial da folha mostrou atividade contra P. aeruginosa e S. pneumoniae, e o óleo essencial da flor mostrou atividade contra P. aeruginosa, S. pneumoniae e Candida albicans.583588Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

TOPOGRAFIA DOS COLATERAIS CALIBROSOS DO ARCO AÓRTICO DE UM MÃO - PELADA (Procyon cancrivorus GRAY, 1865) (CARNIVORA PROCYONIADAE)

Estudou-se a topografia dos colaterais calibrosos do arco aórtico em um exemplar de Procyon cancrivorus, adulto, macho, proveniente da Fazenda Experimental do Glória, da Universidade Federal de Uberlândia, MG, Brasil, o qual teve seus vasos arteriais injetados com solução corada de Neoprene Latex 450, com posterior fixação em solução aquosa de formol a 10% e submetidos à dissecação. O arco aórtico encontra-se em correspondência à terceira costela, no antímero esquerdo da cavidade torácica. O primeiro ramo calibroso do arco aórtico é o tronco braquiocefálico, situado medialmente à terceira costela, originando as artérias carótida comum esquerda, carótida comum direita e a subclávia direita, em correspondência ao primeiro espaço intercostal. A artéria subclávia esquerda é o segundo ramo emergente do arco aórtico, originando-se medialmente à terceira costela. As artérias subclávias direita e esquerda, cedem os mesmos colaterais em ambos os antímeros, ou seja, tronco costocervical esquerdo (medialmente à primeira costela), tronco costocervical direito (borda cranial da primeira costela), artérias cervical superficial esquerda e cervical superficial direita (borda cranial da primeira costela), artéria torácica interna esquerda (medialmente à segunda costela) e artéria torácica interna direita (borda cranial da primeira costela). The topography of the thick collaterals of the aortic arch in a crab eating raccoon Procyon cancrivorus Gray, 1865, Carnivora Procyoniadae Abstract An anatomical study has been carried out on the topography of the thick collateral branches of the aortic arch in an adult male specimen of raccoon (Procyon cancrivorus) from the Gloria Experimental Farm, Federal University of Uberlandia MG, Brasil. For this purpose, the arterial blood vessels were injected with a ruddy solution of Neoprene Latex 450, fixed by means of a 10% aqueous solution of formaldehyde and then dissected. It has been observed that the aortic arch is in correspondence with the third rib at the left side of the thoracic cavity The first thick branch of the aortic arch is the brachiocephalic trunk which is situaded medially in regard to the third rib, originating the left common carotid artery, the right common carotid artery and the right subclavian artery in correspondence with the first intercostal space. The left subclavian artery is the second emerging branch of the aortic arch originating itself medialy to the third rib. The right and the left subclavian arteries give way to the same collaterals in both sides, as the left costocervical trunk (medially to the first rib), the right costocervical trunk (cranial edge of the first rib), the left and the right superficial cervical arteries (cranial edge of the first rib, the left internal thoracic artery (medially to the second rib), and the right internal thoracic artery (cranial edge of the first rib)

ORIGEM E RAMIFICAÇÕES DAS ARTÉRIAS MESENTÉRICAS CRANIAL E CAUDAL EM TARTARUGA DA AMAZÔNIA Podocnemis expansa Schweigger, 1812, (Testudinata-pelomedusidae)

Foram estudadas cinco Podocnemis expansa, fêmeas, cujo peso variava de 550 a 850 g. Os animais tiveram seu sistema arterial injetado com solução corada de látex sintético, fixados e armazenados em solução aquosa de formaldeído a 10%. Após abertura da cavidade celomática, as artérias mesentéricas cranial e caudal foram dissecadas. Encontrou-se a artéria mesentérica cranial como um ramo do tronco celíaco-mesentérico e originando as artérias pancreaticoduodenal caudal, ileocólica e jejunais, que irrigam o duodeno, pâncreas, cólon, íleo e jejuno, respectivamente. A artéria mesentérica caudal origina-se da aorta (60%) ou da artéria ilíaca comum (40%), distribuindo-se para o cólon e reto. Origin and ramifications of the cranial and caudal mesenteric arteries in Amazonian turtle - Podocnemis expansa Schweigger, 1812, Testudinata-Pelomedusidae Abstract An anatomic study has been carried out on the mesenteric arteries of five Podocnemis expansa females weighing from 550 to 680 g. The animals had their artery system injected with a latex solution colored with a specific pigment and were then fixed in a 10% formol solution. After the coelomatic cavity being opened the cranial and caudal mesenteric arteries were dissected. It has been found that the cranial mesenteric artery is a branch of the coeliac mesenteric trunk while the cranial mesenteric artery gives off the pancreatic-duodenal , ileocolic and jejunal arteries that irrigate the duodenum, pancreas, colon, ileum and jejunum respectively. The aorta gives origen of as much as 60% of the caudal mesenteric artery, the remaining 40 % being originated from the common ileac artery, their branches reaching the colon and the rectum

Search for a W' boson decaying to a bottom quark and a top quark in pp collisions at sqrt(s) = 7 TeV

Results are presented from a search for a W' boson using a dataset corresponding to 5.0 inverse femtobarns of integrated luminosity collected during 2011 by the CMS experiment at the LHC in pp collisions at sqrt(s)=7 TeV. The W' boson is modeled as a heavy W boson, but different scenarios for the couplings to fermions are considered, involving both left-handed and right-handed chiral projections of the fermions, as well as an arbitrary mixture of the two. The search is performed in the decay channel W' to t b, leading to a final state signature with a single lepton (e, mu), missing transverse energy, and jets, at least one of which is tagged as a b-jet. A W' boson that couples to fermions with the same coupling constant as the W, but to the right-handed rather than left-handed chiral projections, is excluded for masses below 1.85 TeV at the 95% confidence level. For the first time using LHC data, constraints on the W' gauge coupling for a set of left- and right-handed coupling combinations have been placed. These results represent a significant improvement over previously published limits.Comment: Submitted to Physics Letters B. Replaced with version publishe

Search for the standard model Higgs boson decaying into two photons in pp collisions at sqrt(s)=7 TeV

A search for a Higgs boson decaying into two photons is described. The analysis is performed using a dataset recorded by the CMS experiment at the LHC from pp collisions at a centre-of-mass energy of 7 TeV, which corresponds to an integrated luminosity of 4.8 inverse femtobarns. Limits are set on the cross section of the standard model Higgs boson decaying to two photons. The expected exclusion limit at 95% confidence level is between 1.4 and 2.4 times the standard model cross section in the mass range between 110 and 150 GeV. The analysis of the data excludes, at 95% confidence level, the standard model Higgs boson decaying into two photons in the mass range 128 to 132 GeV. The largest excess of events above the expected standard model background is observed for a Higgs boson mass hypothesis of 124 GeV with a local significance of 3.1 sigma. The global significance of observing an excess with a local significance greater than 3.1 sigma anywhere in the search range 110-150 GeV is estimated to be 1.8 sigma. More data are required to ascertain the origin of this excess.Comment: Submitted to Physics Letters