Perceptions, preferences and barriers: A qualitative study of greenspace and under-representation in Leeds, UK

1. Greenspaces facilitate well-being benefits for humans in several ways including through cognitive restoration, physical exercise and social interaction. However, some groups are under-represented in greenspaces, including women, older people, those with health conditions, people with lower socioeconomic status and people from ethnic minority backgrounds, and so are less likely to accrue these benefits. / 2. Using thematic analysis and semi-structured interviews with 40 individuals from under-represented groups in Leeds, UK, we explore (1) a range of perceived barriers to greenspace access, (2) how spending time in greenspace contributes to well-being for these groups, (3) the perceived positive and negative aspects of greenspace, (4) what impact COVID-19 had on access to greenspace and (5) how greenspaces could be improved. / 3. We also highlight inter-group differences and how some barriers disproportionately affect some of the groups in this study. Safety concerns were particularly important for women and people with low incomes, which included problems with anti-social behaviour (e.g. incivilities and disorder). Cultural barriers were also evident with ethnic minority participants often citing concerns about dogs and issues with visibility and prejudice. Participants desired physical improvements to the quality of greenspaces, along with easier access and transport options, changes in policy regarding dogs and increased security and park wardens to limit anti-social behaviour. / 4. We argue that to increase visitation for under-represented groups, upgrades in the physical environment must be coupled with harnessing community involvement and co-design. Some group differences and tensions in greenspaces, and problems with anti-social behaviours and safety concerns might be limited by more considerate planning and incorporating research findings that address these tensions through intergroup contact

Perceptions, preferences and barriers: A qualitative study of greenspace and under-representation in Leeds, UK

AbstractGreenspaces facilitate well‐being benefits for humans in several ways including through cognitive restoration, physical exercise and social interaction. However, some groups are under‐represented in greenspaces, including women, older people, those with health conditions, people with lower socioeconomic status and people from ethnic minority backgrounds, and so are less likely to accrue these benefits.Using thematic analysis and semi‐structured interviews with 40 individuals from under‐represented groups in Leeds, UK, we explore (1) a range of perceived barriers to greenspace access, (2) how spending time in greenspace contributes to well‐being for these groups, (3) the perceived positive and negative aspects of greenspace, (4) what impact COVID‐19 had on access to greenspace and (5) how greenspaces could be improved.We also highlight inter‐group differences and how some barriers disproportionately affect some of the groups in this study. Safety concerns were particularly important for women and people with low incomes, which included problems with anti‐social behaviour (e.g. incivilities and disorder). Cultural barriers were also evident with ethnic minority participants often citing concerns about dogs and issues with visibility and prejudice. Participants desired physical improvements to the quality of greenspaces, along with easier access and transport options, changes in policy regarding dogs and increased security and park wardens to limit anti‐social behaviour.We argue that to increase visitation for under‐represented groups, upgrades in the physical environment must be coupled with harnessing community involvement and co‐design. Some group differences and tensions in greenspaces, and problems with anti‐social behaviours and safety concerns might be limited by more considerate planning and incorporating research findings that address these tensions through intergroup contact.Read the freePlain Language Summaryfor this article on the Journal blog.</jats:p

The genomes of two key bumblebee species with primitive eusocial organization

Background: The shift from solitary to social behavior is one of the major evolutionary transitions. Primitively eusocial bumblebees are uniquely placed to illuminate the evolution of highly eusocial insect societies. Bumblebees are also invaluable natural and agricultural pollinators, and there is widespread concern over recent population declines in some species. High-quality genomic data will inform key aspects of bumblebee biology, including susceptibility to implicated population viability threats. Results: We report the high quality draft genome sequences of Bombus terrestris and Bombus impatiens, two ecologically dominant bumblebees and widely utilized study species. Comparing these new genomes to those of the highly eusocial honeybee Apis mellifera and other Hymenoptera, we identify deeply conserved similarities, as well as novelties key to the biology of these organisms. Some honeybee genome features thought to underpin advanced eusociality are also present in bumblebees, indicating an earlier evolution in the bee lineage. Xenobiotic detoxification and immune genes are similarly depauperate in bumblebees and honeybees, and multiple categories of genes linked to social organization, including development and behavior, show high conservation. Key differences identified include a bias in bumblebee chemoreception towards gustation from olfaction, and striking differences in microRNAs, potentially responsible for gene regulation underlying social and other traits. Conclusions: These two bumblebee genomes provide a foundation for post-genomic research on these key pollinators and insect societies. Overall, gene repertoires suggest that the route to advanced eusociality in bees was mediated by many small changes in many genes and processes, and not by notable expansion or depauperation

Superhelical Duplex Destabilization and the Recombination Position Effect

The susceptibility to recombination of a plasmid inserted into a chromosome varies with its genomic position. This recombination position effect is known to correlate with the average G+C content of the flanking sequences. Here we propose that this effect could be mediated by changes in the susceptibility to superhelical duplex destabilization that would occur. We use standard nonparametric statistical tests, regression analysis and principal component analysis to identify statistically significant differences in the destabilization profiles calculated for the plasmid in different contexts, and correlate the results with their measured recombination rates. We show that the flanking sequences significantly affect the free energy of denaturation at specific sites interior to the plasmid. These changes correlate well with experimentally measured variations of the recombination rates within the plasmid. This correlation of recombination rate with superhelical destabilization properties of the inserted plasmid DNA is stronger than that with average G+C content of the flanking sequences. This model suggests a possible mechanism by which flanking sequence base composition, which is not itself a context-dependent attribute, can affect recombination rates at positions within the plasmid

The Carbon Assimilation Network in Escherichia coli Is Densely Connected and Largely Sign-Determined by Directions of Metabolic Fluxes

Gene regulatory networks consist of direct interactions but also include indirect interactions mediated by metabolites and signaling molecules. We describe how these indirect interactions can be derived from a model of the underlying biochemical reaction network, using weak time-scale assumptions in combination with sensitivity criteria from metabolic control analysis. We apply this approach to a model of the carbon assimilation network in Escherichia coli. Our results show that the derived gene regulatory network is densely connected, contrary to what is usually assumed. Moreover, the network is largely sign-determined, meaning that the signs of the indirect interactions are fixed by the flux directions of biochemical reactions, independently of specific parameter values and rate laws. An inversion of the fluxes following a change in growth conditions may affect the signs of the indirect interactions though. This leads to a feedback structure that is at the same time robust to changes in the kinetic properties of enzymes and that has the flexibility to accommodate radical changes in the environment

Scintillation light detection in the 6-m drift-length ProtoDUNE Dual Phase liquid argon TPC

DUNE is a dual-site experiment for long-baseline neutrino oscillation studies, neutrino astrophysics and nucleon decay searches. ProtoDUNE Dual Phase (DP) is a 6  ×  6  ×  6 m 3 liquid argon time-projection-chamber (LArTPC) that recorded cosmic-muon data at the CERN Neutrino Platform in 2019-2020 as a prototype of the DUNE Far Detector. Charged particles propagating through the LArTPC produce ionization and scintillation light. The scintillation light signal in these detectors can provide the trigger for non-beam events. In addition, it adds precise timing capabilities and improves the calorimetry measurements. In ProtoDUNE-DP, scintillation and electroluminescence light produced by cosmic muons in the LArTPC is collected by photomultiplier tubes placed up to 7 m away from the ionizing track. In this paper, the ProtoDUNE-DP photon detection system performance is evaluated with a particular focus on the different wavelength shifters, such as PEN and TPB, and the use of Xe-doped LAr, considering its future use in giant LArTPCs. The scintillation light production and propagation processes are analyzed and a comparison of simulation to data is performed, improving understanding of the liquid argon properties

Design, construction and operation of the ProtoDUNE-SP Liquid Argon TPC

The ProtoDUNE-SP detector is a single-phase liquid argon time projection chamber (LArTPC) that was constructed and operated in the CERN North Area at the end of the H4 beamline. This detector is a prototype for the first far detector module of the Deep Underground Neutrino Experiment (DUNE), which will be constructed at the Sandford Underground Research Facility (SURF) in Lead, South Dakota, U.S.A. The ProtoDUNE-SP detector incorporates full-size components as designed for DUNE and has an active volume of 7 × 6 × 7.2 m3. The H4 beam delivers incident particles with well-measured momenta and high-purity particle identification. ProtoDUNE-SP's successful operation between 2018 and 2020 demonstrates the effectiveness of the single-phase far detector design. This paper describes the design, construction, assembly and operation of the detector components