Presence of human papillomavirus DNA in squamous cell carcinoma biopsies in adults and elderly with head and neck cancer

Orientadores: Sandra Cecília Botelho Costa, Sandra Helena Alves BononTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciências MédicasResumo: Mesmo com avançadas modalidades terapêuticas, o carcinoma espinocelular se mostra como responsável pelo aumento da taxa de mortalidade da maioria dos pacientes diagnosticados com neoplasias de cabeça e pescoço. Tumores de cabeça e pescoço associados ao vírus HPV se comportam de modo diferente dos demais. A replicação do HPV ocorre no núcleo das células escamosas e seu ciclo de vida está diretamente relacionado ao programa de diferenciação celular da célula hospedeira. Por outro lado, o câncer causa um estado de imunodepressão, que favorece a ação de agentes infecciosos oportunistas, de modo que reativações virais são comuns em pacientes adultos e idosos. Objetivo: avaliar a prevalência do papilomavírus humano (HPV) em biópsias de tecidos neoplásicos obtidos de pacientes com diagnóstico de neoplasias de cabeça e pescoço. Método: foram selecionados um total de sessenta e oito biópsias de tecidos à fresco e parafinadas. Utilizou-se de técnicas de Biologia Molecular como a Reação em Cadeia da Polimerase tipo nested (n-PCR) para a detecção do DNA viral. A pesquisa foi submetida e aprovada pelo Comitê de Ética em Pesquisa da FCM/Unicamp e o TCLE fornecido e assinado por todos os participantes. Os dados obtidos foram tabulados, organizados e armazenados em planilhas do Programa Office Excel e submetidos à análise estatística. O nível de significância para todas as análises foi de 5%, ou valores de p<0,05. Resultados: treze amostras foram positivas para a presença do DNA de HPV (19,12%), sendo sete casos para as amostras à fresco (35%) e seis para as amostras parafinadas (12,5%). Entre as amostras à fresco (n=20), a maioria dos casos positivos era constituída por homens, brancos e casados com média de idade de 53,3 anos. Quanto à localização, 71,4% dos tumores eram de laringe, classificados como moderadamente diferenciados e se apresentavam no estágio IV de estadiamento, tendo cirurgia, quimio e radioterapia como procedimentos terapêuticos. Todos possuíam história de tabagismo e 85,5% de etilismo. As amostras parafinadas (n=48), apontaram a média de idade de 68,5 anos. A maioria (83,3%) composta por homens, casados (66,6%) e brancos. Em 50% dos casos, a região mais atingida foi a laringe e 83,3% como moderadamente diferenciados. Quanto ao estadiamento, 50% foram classificados no estágio III, apresentando a cirurgia de ressecção do tumor como medida terapêutica mais utilizada. Todos reportaram tabagismo e 66,6% etilismo. A proporção de HPV positivo foi maior entre a população adulta e os achados histopatológicos mostraram a presença de células com coilocitose em ambas as amostras. Conclusão: o DNA do vírus HPV foi mais prevalente em neoplasias de laringe e cavidade oral, cujos tecidos se apresentavam como moderadamente diferenciados. O diagnóstico tardio e o início do tratamento em estágios avançados da doença indicam a necessidade de planejamento de ações de intervenção e/ou educativas precoces com a finalidade de diminuir os efeitos negativos de eventos que possam ocorrer com o avanço da doença, permitindo a possibilidade de cura e a melhoria da qualidade de vida destes pacientesAbstract: Even with advanced therapeutic modalities, squamous cell carcinoma is shown to be responsible for the increased mortality rate of the majority of patients diagnosed with head and neck neoplasms. Tumors of head and neck associated with the HPV virus behave differently from the others. HPV replication occurs in the nucleus of squamous cells and its life cycle is directly related to the cell differentiation program of the host cell. On the other hand, cancer causes a state of immunodepression, which favors the action of opportunistic infections agents, so that viral reactivations are common in adult and elderly patients. Objective: to evaluate the prevalence of human papillomavirus (HPV) in neoplastic tissue biopsies obtained from patients with head and neck neoplasms. Method: a total of sixty-eight fresh tissue and paraffin-embedded biopsies were selected from patients aged 41-90 years. Molecular Biology techniques such as the nested-type Polymerase Chain Reaction (nested-PCR) were used to detect the viral DNA. The research was submitted and approved by the Research Ethics Committee of FCM/Unicamp and the TCLE provided and signed by all participants. The data obtained were tabulated, organized and stored in Excel Program spreadsheets and submitted to statistical analysis. The level of significance for all analyzes was 5%, or p values<0.05. Results: thirteen samples were positive for the presence of HPV DNA (19.12%), seven cases for fresh samples (35%) and six for paraffin samples (12.5%). Among fresh samples (n=20), the majority of the positive cases consisted of men, white and married, with a mean age of 53.3 years. As to the localization, 71.4% of the tumors were larynx, classified as moderately differentiated and presented in staging stage IV, with surgery, chemo and radiotherapy as therapeutic procedures. All had a history of smoking and 85.5% of alcoholism. The paraffined samples (n=48), showed the mean age of 68.5 years. The majority (83.3%) were man, married (66.6%) and white. In a 50% of the cases, the region most affected was the larynx and 83.3% as moderately differentiated. Regarding staging, 50% were classified in stage III, presenting tumor resection surgery as the most used therapeutic measure. All reported smoking and 66.6% alcohol consumption. The proportion of HPV positive was higher among the adult population and the histopathological findings showed the presence of cells with koilocytosis in both samples. Conclusion: The HPV virus DNA was more prevalent in laryngeal and oral cavity neoplasms, whose tissues were moderately differentiated. The late diagnosis and the beginning of treatment in advanced stages of the disease indicate the need to plan early intervention and/or educational actions with the purpose of reducing the negative effects of events that may occur the disease progression, allowing the possibility of cure and improvement of the quality of life of these patientsDoutoradoGerontologiaDoutora em Gerontologia1P-3888/2011CAPE

Autopercepção da saúde bucal em idosos e fatores associados em Campinas, SP, 2008-2009

OBJECTIVE: To describe self-perceived oral health among elderly people and assess associated sociodemographic and clinical factors. METHODS: A cross-sectional study was carried out with 876 participants forming a representative sample of elderly people (65 years of age or over) in Campinas, Southeastern Brazil, in 2008-2009. Dental examinations were conducted in accordance with criteria standardized by the World Health Organization for epidemiological surveys on oral health. Self-perceived oral health was evaluated using the Geriatric Oral Health Assessment Index (GOHAI). Individuals were classified according to sociodemographic characteristics, dental factors and prevalence of biological frailty. Associations were assessed using Poisson regression analysis, taking into consideration sample weights and the complex structure of the cluster sampling. RESULTS: The subjects' mean age was 72.8 years; 70.1% were women. The proportion of the individuals with more than 20 teeth present was 17.2%; 38.2% were using full dentures in both arches; 8.5% needed dental prostheses in at least one arch. On average, the GOHAI was high: 33.9 (maximum possible: 36.0). Retaining 20 teeth or more, using full dentures in both arches, not needing such treatment, not presenting any oral mucosa abnormalities and not presenting biological frailty were factors significantly associated with better self-perceived oral health (p < 0.05). CONCLUSIONS: Assessment of self-perceived oral health made it possible to identify the main factors associated with this outcome. This tool may contribute towards planning dental services and guide health promotion strategies for improving the quality of life of individuals within this age group.OBJETIVO: Describir la autopercepción de salud bucal en ancianos y analizar factores sociodemográficos y clínicos asociados. MÉTODOS: Estudio transversal con 876 participantes en muestra representativa de ancianos (65 años o más) de Campinas, Sureste de Brasil, en 2008-2009. Los exámenes odontológicos siguieron criterios estandarizados por la Organización Mundial de la Salud para levantamientos epidemiológicos de salud bucal. La autopercepción de la salud bucal fue evaluada por el índice Geriatric Oral Health Assessment Index (GOHAI). Los individuos fueron clasificados según características sociodemográficas, odontológicas y prevalencia de fragilidad biológica. El estudio de asociaciones utilizó análisis de regresión de Poisson; el análisis consideró los pesos muestrales y la estructura compleja de la muestra por conglomerados. RESULTADOS: El promedio de edad de los individuos fuese 72,8 años; 70,1% eran mujeres. La proporción de individuos congas de 20 dientes presentes fue 17,2%; 38,2% usaban prótesis dentaria total en ambos arcos; 8,5% necesitaban de tal recurso en al menos un arco dentario. En promedio, el indice GOHAI fue elevado: 33,9 (máximo posible 36,0). Mantener 20 dientes o más, usar prótesis total en los dos arcos, no necesitar de este tratamiento, no presentar alteraciones de mucosa oral y no presentar fragilidad biológica fueron los factores significativamente asociados con mejor autopercepción de salud bucal (pOBJETIVO: Descrever a autopercepção de saúde bucal em idosos e analisar fatores sociodemográficos e clínicos associados. MÉTODOS: Estudo transversal com 876 participantes em amostra representativa de idosos (65 anos ou mais) de Campinas, SP, em 2008-2009. Os exames odontológicos seguiram critérios padronizados pela Organização Mundial da Saúde para levantamentos epidemiológicos de saúde bucal. A autopercepção da saúde bucal foi avaliada pelo índice Geriatric Oral Health Assessment Index (GOHAI). Os indivíduos foram classificados segundo características sociodemográficas, odontológicas e prevalência de fragilidade biológica. O estudo de associações utilizou análise de regressão de Poisson; a análise considerou os pesos amostrais e a estrutura complexa da amostra por conglomerados. RESULTADOS: A média de idade dos indivíduos foi de 72,8 anos; 70,1% eram mulheres. A proporção de indivíduos com mais de 20 dentes presentes foi 17,2%; 38,2% usavam prótese dentária total em ambos os arcos; 8,5% necessitavam desse recurso em ao menos um arco dentário. Em média, o índice GOHAI foi elevado: 33,9 (máximo possível 36,0). Manter 20 dentes ou mais, usar prótese total nos dois arcos, não necessitar desse tratamento, não apresentar alterações de mucosa oral e não apresentar fragilidade biológica foram os fatores significantemente associados com melhor autopercepção de saúde bucal (p < 0,05). CONCLUSÕES: A avaliação de autopercepção em saúde bucal permitiu identificar os principais fatores associados a esse desfecho. Esse instrumento pode contribuir para o planejamento de serviços odontológicos, orientando estratégias de promoção em saúde voltadas à melhora da qualidade de vida das pessoas desse grupo etário

Gaia Early Data Release 3: Summary of the contents and survey properties

ABSTRACT: Context. We present the early installment of the third Gaia data release, Gaia EDR3, consisting of astrometry and photometry for 1.8 billion sources brighter than magnitude 21, complemented with the list of radial velocities from Gaia DR2. Aims. A summary of the contents of Gaia EDR3 is presented, accompanied by a discussion on the differences with respect to Gaia DR2 and an overview of the main limitations which are present in the survey. Recommendations are made on the responsible use of Gaia EDR3 results. Methods. The raw data collected with the Gaia instruments during the first 34 months of the mission have been processed by the Gaia Data Processing and Analysis Consortium and turned into this early third data release, which represents a major advance with respect to Gaia DR2 in terms of astrometric and photometric precision, accuracy, and homogeneity. Results. Gaia EDR3 contains celestial positions and the apparent brightness in G for approximately 1.8 billion sources. For 1.5 billion of those sources, parallaxes, proper motions, and the (GBP ? GRP) colour are also available. The passbands for G, GBP, and GRP are provided as part of the release. For ease of use, the 7 million radial velocities from Gaia DR2 are included in this release, after the removal of a small number of spurious values. New radial velocities will appear as part of Gaia DR3. Finally, Gaia EDR3 represents an updated materialisation of the celestial reference frame (CRF) in the optical, the Gaia-CRF3, which is based solely on extragalactic sources. The creation of the source list for Gaia EDR3 includes enhancements that make it more robust with respect to high proper motion stars, and the disturbing effects of spurious and partially resolved sources. The source list is largely the same as that for Gaia DR2, but it does feature new sources and there are some notable changes. The source list will not change for Gaia DR3. Conclusions. Gaia EDR3 represents a significant advance over Gaia DR2, with parallax precisions increased by 30 per cent, proper motion precisions increased by a factor of 2, and the systematic errors in the astrometry suppressed by 30-40% for the parallaxes and by a factor ~2.5 for the proper motions. The photometry also features increased precision, but above all much better homogeneity across colour, magnitude, and celestial position. A single passband for G, GBP, and GRP is valid over the entire magnitude and colour range, with no systematics above the 1% levelThe Gaia mission and data processing have financially been supported by ; the Spanish Ministry of Economy (MINECO/FEDER, UE) through grants ESP2016-80079-C2-1-R, ESP2016-80079-C2-2-R, RTI2018-095076-B-C21, RTI2018-095076-B-C22, BES-2016-078499, and BES-2017-083126 and the Juan de la Cierva formación 2015 grant FJCI-2015-2671, the Spanish Ministry of Education, Culture, and Sports through grant FPU16/03827, the Spanish Ministry of Science and Innovation (MICINN) through grant AYA2017-89841P for project “Estudio de las propiedades de los fósiles estelares en el entorno del Grupo Local” and through grant TIN2015-65316-P for project “Computación de Altas Prestaciones VII

Gaia Early Data Release 3 Acceleration of the Solar System from Gaia astrometry

Context. Gaia Early Data Release 3 (Gaia EDR3) provides accurate astrometry for about 1.6 million compact (QSO-like) extragalactic sources, 1.2 million of which have the best-quality five-parameter astrometric solutions. Aims. The proper motions of QSO-like sources are used to reveal a systematic pattern due to the acceleration of the solar systembarycentre with respect to the rest frame of the Universe. Apart from being an important scientific result by itself, the acceleration measured in this way is a good quality indicator of the Gaia astrometric solution. Methods. Theeffect of the acceleration was obtained as a part of the general expansion of the vector field of proper motions in vector spherical harmonics (VSH). Various versions of the VSH fit and various subsets of the sources were tried and compared to get the most consistent result and a realistic estimate of its uncertainty. Additional tests with the Gaia astrometric solution were used to get a better idea of the possible systematic errors in the estimate. Results. Our best estimate of the acceleration based on Gaia EDR3 is (2.32 +/- 0.16) x 10(-10) m s(-2) (or 7.33 +/- 0.51 km s(-1) Myr-1) towards alpha = 269.1 degrees +/- 5.4 degrees, delta = -31.6 degrees +/- 4.1 degrees, corresponding to a proper motion amplitude of 5.05 +/- 0.35 mu as yr(-1). This is in good agreement with the acceleration expected from current models of the Galactic gravitational potential. We expect that future Gaia data releases will provide estimates of the acceleration with uncertainties substantially below 0.1 mu as yr(-1).Peer reviewe

A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

Meeting abstrac

Gaia Early Data Release 3: acceleration of the solar system from Gaia astrometry

Stars and planetary system

Gaia early data release 3: summary of the contents and survey properties (Corrigendum)

ERRATUMThis article is an erratum for:[https://doi.org/10.1051/0004-6361/202039657]​​​​​​​Instrumentatio

Gaia Early Data Release 3: the Gaia catalogue of nearby stars

Stars and planetary system

Gaia early data release 3: structure and properties of the Magellanic Clouds

Galaxie

Gaia Early Data Release 3: the Galactic anticentre

Stars and planetary system