Parameters of the Reproductive Tract, Spermatogenesis, Daily Sperm Production and Major Seminal Plasma Proteins of Tropically Adapted Morada Nova Rams

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Contents This study describes the reproductive parameters of Morada Nova rams, a breed of hair sheep from Brazil and with unique adaption to tropical environments. At 42weeks of age, 15 rams were subjected to semen collection and, 1week later, animals were slaughtered for collection of testes, epididymis and accessory sex glands. We conducted 2-D electrophoresis of seminal plasma proteins and major spots of stained gels were identified by LC-MS/MS. Total RNA was isolated from testis, epididymis and vesicular glands and subjected to qPCR. At slaughter, scrotal circumference and testicular weight were 27.5 +/- 0.5cm and 109.5 +/- 6.0g, respectively. Seminiferous tubule (ST) diameter was 188.3 +/- 4.0m and each testis contained 1.9 +/- 0.1 Sertoli cells (x10(9)). Each Sertoli cell supported 0.1 +/- 0.01 A spermatogonia, 3.0 +/- 0.2 pachytene spermatocytes and 7.7 +/- 0.5 round spermatids/tubule cross section. Daily sperm production reached 5.6x10(6)cells/g of testis parenchyma. Testis size appeared as indicative of ST diameter and associated with epididymal measurements, as well as with the population of round spermatids and Sertoli cells/testis. Rams with heavier testes had greater daily sperm production and more Sertoli cells/testis. We detected 90.9 +/- 9.6 spots per 2-D gel of seminal plasma. Major seminal proteins were identified as ram seminal vesicle proteins at 14 and 22kDa, representing 16.2% and 12.8% of the total intensity of valid spots in the gels, respectively. Expression of both genes was greater in the vesicular glands as compared to testis and epididymis. Pixel intensity for those proteins in the 2-D gels was significantly correlated with seminal vesicle weight. This is the first description of the basic reproductive aspects of Morada Nova rams, including protein profiles of their seminal plasma. These findings will allow a better understanding of their reproductive physiology.493409419Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Ceara State Agency for Technology and Scientific Development (FUNCAP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

Cuidado nutricional hospitalar: percepção de nutricionistas para atendimento humanizado Hospital nutritional care: perception of the nutritionist for humanized attendance

A pesquisa identificou as ações de cuidado alimentar e nutricional considerando as perspectivas da equipe de nutricionistas de um hospital de referência para a Política Nacional de Humanização (PNH). A partir de uma abordagem qualitativa, utilizou-se a técnica de grupos focais. A pesquisa envolveu treze nutricionistas, que foram divididas em dois grupos de acordo com o tempo de serviço, seguindo critérios de homogeneidade. As entrevistas foram desenvolvidas para análise da categoria: ser nutricionista para um atendimento humanizado, buscando-se conhecer as seguintes ações da prática destes profissionais: avaliação nutricional do paciente; planejamento, implementação e avaliação do cuidado nutricional e alimentar. A análise do conteúdo foi utilizada como técnica para sistematização das informações obtidas, agrupando-as em unidades de significado. O estudo revelou que há priorização do atendimento individualizado em função do número de leitos e de atividades burocráticas, falta de autonomia em relação à prescrição de dietas, dificuldades de interação com outros profissionais de saúde e entre as nutricionistas das áreas clínica e produção de refeições. Os resultados deste estudo poderão oferecer aos profissionais subsídios que fundamentem ações para a construção de um modelo de cuidado alimentar e nutricional humanizado em hospitais.<br>The research analyzed the actions of alimentary and nutritional care considering the perspectives of the nutritionists in a hospital reference for the National Politics of Humanization (PNH). From a qualitative approach, a focal group technique was used. The nutritionists were divided in two groups by working time, following homogeneity criteria. The interviews were developed for analysis of the category: Being a nutritionist for a humanized assistance, seeking to understand these professionals following actions: nutritional evaluation of the patient; planning, implementation and evaluation of the nutritional and alimentary care. The analysis of the content was used as a technique for the systematization of the collected information grouped in units of meaning. The study disclosed that there is prioritization of the individualized assistance in function of the number of beds and bureaucratic activities, lack of autonomy in relation to the prescription of diets, difficulties of interaction with other health professionals and between the nutritionists of the clinical and meal production areas. The results will provide the professional subsidies that substantiate actions for the construction of a model of humanized alimentary and nutritional care on hospitals

Resultados de Pesquisa em Nutrição de Aves no Brasil: Resumo dos Ultimos 5 anos Results of Poultry Nutrition Research in Brazil: A Summary of the Last 5 years

Foi realizada uma revisão bibliográfica sobre as pesquisas brasileiras na área de nutrição avícola, entre 1994 a l999. Nesse período, foi publicado um total de 496 trabalhos, com uma média de 82,7 trabalhos/ ano. Os trabalhos na área de nutrição revelaram que 64,52% foram realizados com frangos de corte; 24,19%, com poedoiras e aves de reposição; 3,93%, com matrizes pesadas; e 7,66%, com outras aves. Com relação às publicações em forma de resumos expandidos, foram publicados 226 e 163 resumos, nos Anais das Reuniões Anuais da Fundação APINCO de Ciência e Tecnologia Avícolas e da Sociedade Brasileira de Zootecnia, respectivamente. Na Revista da Sociedade Brasileira de Zootecnia e no Arquivo Brasileiro de Medicina Veterinária e Zootecnia foram publicados, respectivamente, 65 e 34 trabalhos completos. Os pesquisadores das instituições UFV, UNESP-Jaboticabal e UFRGS publicaram no total 104, 92 e 46 trabalhos. As pesquisas concentraram-se principalmente nas áreas de proteína/aminoácidos (21,77%), avaliação de alimentos (21,77%), minerais (19,15%) e aditivos (16,33%).<br>A literature review of the papers published in the area of poultry nutrition was done considering Brazilian publications from the period of 1994 to 1999. During this period a total of 496 papers were published, with an average of 82. 7 papers/year. The publications in nutrition revealed that 64. 52% were made with broiler chickens, 24. 19% with laying hens and replacement birds, 3.93% with broiler breeders and 7.66% with other type of birds. Considering the publications in the form of summary, 226 and 163, were published in the Proceedings of Fundação APINCO de Ciência e Tecnologia Avícola and Sociedade Brasileira de Zootecnia, respectively. In the Revista da Sociedade Brasileira de Zootecnia and Arquivo Brasileiro de Medicina Veterinária e Zootecnia were published, 65 and 34 papers, respectively. Researches in the Universities UFV, UNESP-Jaboticabal and UFRGS, accounted for a total 104, 92 and 46 papers, respectively. The papers in poultry nutrition concentrated mainly in the area of protein/amino acid (21.77%), feedstuffs evaluation (21.77%), minerals (19.15%) and additives (16.33%)

Search for high-mass resonances decaying to dilepton final states in pppp collisions at s=7\sqrt{s}= 7 TeV with the ATLAS detector

The ATLAS detector at the Large Hadron Collider is used to search for high-mass resonances decaying to an electron-positron pair or a muon-antimuon pair. The search is sensitive to heavy neutral Z' gauge bosons, Randall-Sundrum gravitons, Z* bosons, techni-mesons, Kaluza-Klein Z/gamma bosons, and bosons predicted by Torsion models. Results are presented based on an analysis of pp collisions at a center-of-mass energy of 7 TeV corresponding to an integrated luminosity of 4.9/fb in the dielectron channel and 5.0/fb in the dimuon channel. A Z' boson with Standard Model-like couplings is excluded at 95 percent confidence level for masses below 2.22 TeV. A Randall-Sundrum graviton with coupling k/Mbar = 0.1 is excluded at 95 percent confidence level for masses below 2.16 TeV. Limits on the other models are also presented, including Technicolor and Minimal Z' Models.Comment: 31 pages plus author list (53 pages total), 10 figures, 16 tables, submitted to Journal of High Energy Physics Revision corresponds to published versio