MetaboRank: network-based recommendation system to interpret and enrich metabolomics results

International audienceMetabolomics has shown great potential to improve the understanding of complex diseases, potentially leading to therapeutic target identification. However, no single analytical method allows monitoring all metabolites in a sample, resulting in incomplete metabolic fingerprints. This incompleteness constitutes a stumbling block to interpretation, raising the need for methods that can enrich those fingerprints. We propose MetaboRank, a new solution inspired by social network recommendation systems for the identification of metabolites potentially related to a metabolic fingerprint. MetaboRank method had been used to enrich metabolomics data obtained on cerebrospinal fluid samples from patients suffering from hepatic encephalopathy. MetaboRank successfully recommended metabolites not present in the original fingerprint. The quality of recommendations was evaluated by using literature automatic search, in order to check that recommended metabolites could be related to the disease. Complementary mass spectrometry experiments and raw data analysis were performed to confirm these suggestions. In particular, MetaboRank recommended the overlooked α-ketoglutaramate as a metabolite which should be added to the metabolic fingerprint of hepatic encephalopathy, thus suggesting that metabolic fingerprints enhancement can provide new insight on complex diseases

Fog Analysis during SOFOG3D Experiment

International audienceTransportation especially aviation sector all around the world is severely hindered due to Fog and hence observations and specific research for fog is necessary. The SOFOG3D (SOuth west FOGs 3D) experiment took place in South-West of France which is particularly prone to fog occurrence, during the period between November 2019 to March 2020 with primary objective to advance our understanding of fog processes and to improve fog forecast. Simultaneous measurements from various remote sensing instruments like BASTA: a 95 GHz cloud radar with scanning capability, HATPRO Microwave radiometer (MWR), doppler lidar, and balloon-borne in-situ measurements were collected to characterize the spatio-temporal evolution of Fog. On the supersite, detailed measurements of meteorological conditions, aerosol properties, fog microphysics, water deposition, radiation budget, heat, and momentum fluxes are collected to provide 3D structure of the boundary layer during fog events. The improvement in the retrieval of fog parameters and understanding of fog dynamics based on cloud radar and microwave (MWR) synergy will be addressed. We will present our work on the retrieval of key fog parameters like dynamics and microphysics using a combination of cloud radar and MWR observations. The retrievals will be validated with the tethered-balloon and radio-sounding observations. In-situ measurements and remote-sensing retrievals of fog microphysical properties will be compared. We will show a detailed analysis of retrieved LWP derived from BASTA radar only with LWP derived from HATPRO microwave radiometer, considering instrumental uncertainty and sensitivity. A closer analysis of the in-situ data (measured by granulometer) will be presented in order to assess and improve the retrieval derived with cloud radar in vertically pointing mode. Radar attenuation will be quantified by measuring the backscattered radar signal on well-known calibrated reflectivity metallic targets installed at the top of 20 m mast. The integrated attenuation along the radar beam path will be measured by the cloud radar and used as a new constraint to improve the microphysical properties.</p&gt

Combined Immunodeficiency in Patients With Trichohepatoenteric Syndrome

The syndromic diarrhea/trichohepatoenteric syndrome (SD/THE) is a rare and multi-system genetic disorder caused by mutation in SKIV2L or in TTC37, two genes encoding subunits of the putative human SKI complex involved in RNA degradation. The main features are intractable diarrhea of infancy, hair abnormalities, facial dysmorphism, and intrauterine growth restriction. Immunologically this syndrome is associated with a hypogammaglobulinemia leading to an immunoglobulin supplementation. Our immune evaluation of a large French cohort of SD/THE patient revealed several immunological defects. First, switched memory B lymphocytes count is very low. Second, IFN-γ production by T and NK cells is impaired and associated with a reduced degranulation of NK cells. Third, T cell proliferation was abnormal in 3/6 TTC37-mutated patients. These three patients present with severe EBV infection and a transient hemophagocytosis which may be related to these immunological defects. Moreover, an immunological screening of patients with clinical features of SD/THE could facilitate both diagnosis and therapeutic management of these patients

L'insegnamento della lingua italiana all'estero. Francia, Gran Bretagna, Germania, Spagna, Canada, Stati Uniti, Argentina, Brasile, Australia

Vasta rassegna sull'insegnamento dell'italiano nei maggiori paesi europei ed extraeuropei.- Indice #9- Presentazione #17- L’insegnamento dell’italiano in Francia, Adelin Fiorato, Patrick Mariani, Frédéric Vinson #21- L’insegnamento dell’italiano in Gran Bretagna, Laura Lepschy, Brian Richardson, Edward J. T. Williams, Ann Vroudfoof #57- L’insegnamento dell’italiano nella Repubblica federale tedesca, Hermann Neumeister #87- L’insegnamento dell’italiano in Spagna, Bernardo Fáñez Pérez #139- L’insegnamento dell’italiano in Canada, Anthony Mollica #181- L’insegnamento dell’italiano negli Stati Uniti, Edoardo A. Lebano #213- L’insegnamento dell’italiano in Argentina, Carlos Martin Calcopietro, Marta Gabriela Michetti #243- Recenti riforme legislative sull’insegnamento dell’italiano in Argentina e iniziative per la formazione dei docenti, Dea Pellegrini #269- L’insegnamento dell’italiano in Brasile, Euclides Antônio Lazzarotto #303- L’insegnamento dell’italiano in Australia, Camilla Bettoni, Bruno Di Biase #333- Bibliografia #36

The cAMP response element binding protein-2 (CREB-2) can interact with the C/EBP-homologous protein (CHOP).

cAMP response element binding protein-2 (CREB-2) is a basic leucine zipper (bZIP) factor that was originally described as a repressor of CRE-dependent transcription but that can also act as a transcriptional activator. Moreover, CREB-2 is able to function in association with the viral Tax protein as an activator of the human T-cell leukemia virus type I (HTLV-I) promoter. Here we show that CREB-2 is able to interact with C/EBP-homologous protein (CHOP), a bZIP transcription factor known to inhibit CAAT/enhancer-dependent transcription. Cotransfection of CHOP with CREB-2 results in decreased activation driven by the cellular CRE motif or the HTLV-I proximal Tax-responsive element, confirming that CREB-2 and CHOP can interact with each other in vivo

Změna základního tónu řeči a transformace hlasu pomocí PSOLA

In the paper a voice transformation approach based on the application of the Pitch Synchronous OverLap and Add /PSOLA/ principle and resampling is proposed. This algorithm has lower computational demands than frequency domain methods