305 research outputs found

    Integrated Assessment of Genomic Correlates of Protein Evolutionary Rate

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    Rates of evolution differ widely among proteins, but the causes and consequences of such differences remain under debate. With the advent of high-throughput functional genomics, it is now possible to rigorously assess the genomic correlates of protein evolutionary rate. However, dissecting the correlations among evolutionary rate and these genomic features remains a major challenge. Here, we use an integrated probabilistic modeling approach to study genomic correlates of protein evolutionary rate in Saccharomyces cerevisiae. We measure and rank degrees of association between (i) an approximate measure of protein evolutionary rate with high genome coverage, and (ii) a diverse list of protein properties (sequence, structural, functional, network, and phenotypic). We observe, among many statistically significant correlations, that slowly evolving proteins tend to be regulated by more transcription factors, deficient in predicted structural disorder, involved in characteristic biological functions (such as translation), biased in amino acid composition, and are generally more abundant, more essential, and enriched for interaction partners. Many of these results are in agreement with recent studies. In addition, we assess information contribution of different subsets of these protein properties in the task of predicting slowly evolving proteins. We employ a logistic regression model on binned data that is able to account for intercorrelation, non-linearity, and heterogeneity within features. Our model considers features both individually and in natural ensembles (“meta-features”) in order to assess joint information contribution and degree of contribution independence. Meta-features based on protein abundance and amino acid composition make strong, partially independent contributions to the task of predicting slowly evolving proteins; other meta-features make additional minor contributions. The combination of all meta-features yields predictions comparable to those based on paired species comparisons, and approaching the predictive limit of optimal lineage-insensitive features. Our integrated assessment framework can be readily extended to other correlational analyses at the genome scale

    Validação do questionårio de avaliação funcional das mãos em hanseníase

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    OBJETIVO: Validar as propriedades psicomĂ©tricas do questionĂĄrio de avaliação funcional das mĂŁos em hansenĂ­ase. MÉTODOS: Estudo realizado com amostra de conveniĂȘncia de 101 pacientes consecutivos em BrasĂ­lia, DF, de junho de 2008 a julho de 2009. As pessoas eram adultas afetadas pela hansenĂ­ase, com comprometimento nos nervos ulnar, mediano e radial. Foi analisada a reprodutibilidade interobservadores e intraobservador com entrevistas sucessivas e a validade do constructo com associação entre idade, forma clĂ­nica da hansenĂ­ase, tempo de lesĂŁo do nervo, forças de preensĂŁo e pinças realizadas com dinamĂŽmetro, teste de sensibilidade realizado com monofilamentos de Semmes-Weinstein e avaliação da habilidade manual, utilizando o teste de função manual de Jebsen. Calcularam-se os valores do Ă­ndice kappa ponderado e construiu-se um grĂĄfico Bland-Altman para avaliar a reprodutibilidade do instrumento. Para a consistĂȘncia interna, utilizou-se o coeficiente alfa de Cronbach. Foi calculado o coeficiente de correlação de Pearson e usado modelo de regressĂŁo mĂșltipla. RESULTADOS: Os valores de kappa ponderado para as avaliaçÔes interobservadores e intraobservador variaram de 0,86 a 0,97 e de 0,85 a 0,97, respectivamente. O valor do coeficiente alfa de Cronbach foi de 0,967. O coeficiente de correlação de Pearson mostrou associação (p < 0,001) entre tempo de lesĂŁo do nervo, forças de preensĂŁo e pinças, sensibilidade cutĂąnea e escore mĂ©dio do teste de Jebsen. O escore mĂ©dio do questionĂĄrio de avaliação funcional das mĂŁos em hansenĂ­ase associou-se com classificação operacional da hansenĂ­ase, tempo de lesĂŁo do nervo, força de preensĂŁo, sensibilidade cutĂąnea e habilidade manual (p < 0,0001 para o conjunto do modelo). CONCLUSÕES: O questionĂĄrio de avaliação funcional das mĂŁos em hansenĂ­ase apresenta reprodutibilidade quase perfeita interobservadores e intraobservador, alta consistĂȘncia interna e correlação com classificação operacional da hansenĂ­ase, tempo de lesĂŁo do nervo, força de preensĂŁo, sensibilidade cutĂąnea nas mĂŁos e habilidade manual

    Observation of associated near-side and away-side long-range correlations in √sNN=5.02  TeV proton-lead collisions with the ATLAS detector

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    Two-particle correlations in relative azimuthal angle (Δϕ) and pseudorapidity (Δη) are measured in √sNN=5.02  TeV p+Pb collisions using the ATLAS detector at the LHC. The measurements are performed using approximately 1  Όb-1 of data as a function of transverse momentum (pT) and the transverse energy (ÎŁETPb) summed over 3.1<η<4.9 in the direction of the Pb beam. The correlation function, constructed from charged particles, exhibits a long-range (2<|Δη|<5) “near-side” (Δϕ∌0) correlation that grows rapidly with increasing ÎŁETPb. A long-range “away-side” (Δϕ∌π) correlation, obtained by subtracting the expected contributions from recoiling dijets and other sources estimated using events with small ÎŁETPb, is found to match the near-side correlation in magnitude, shape (in Δη and Δϕ) and ÎŁETPb dependence. The resultant Δϕ correlation is approximately symmetric about π/2, and is consistent with a dominant cos⁥2Δϕ modulation for all ÎŁETPb ranges and particle pT
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