Assessing potential impacts of sea level rise on mangrove ecosystems in the Mekong Delta, Vietnam

Sea level rise (SLR) due to global climate change negatively impacts coastal zones, in particular wetland and mangrove ecosystems. Mangroves in the Mekong Delta (MD) in Vietnam provide critical ecosystem services in the region" however, escalated relative SLR is likely to affect all ecosystems in the region, with mangroves probably more vulnerable than others. Given the fact that documented information and studies on SLR impacts on mangroves are limited for the region, this study aims to investigate potential changes in mangrove distribution in response to future SLR scenarios in the coastal area in the south of the MD using the Sea Level Affects Marshes Model (SLAMM). Wetland maps for 2013 derived from Landsat 8 OLI sensor, digital elevation model (DEM), and localized site-specific parameters (i.e., subsidence/accretion, erosion, historic trend of SLR, and over-wash) were used as input for the SLAMM to simulate spatial distribution of mangroves under different relative SLR scenarios (i.e., RCP2.6, RCP4.5, RCP8.5, more extreme SLR), and surface elevation change (i.e., subsidence, stable, and accretion) scenarios by the year 2100. Simulation results show that the average annual mangrove losses are likely to be 0.54% and 0.22% for subsidence and stable scenarios, respectively. The findings demonstrate the considerable impacts of SLR on MD mangrove ecosystems and the strong influence of subsidence processes. Inundation was also identified as a main driver responsible for the mangrove loss by the end of this century. Our results are in agreement with findings of other studies at global scales and observed data at regional scales. The results also demonstrate the potential of the approach developed herein for simulating mangrove dynamics under future relative SLR scenarios in the region with acceptable accuracy. The findings from the present study are useful sources for development of proper strategies for minimizing the impacts of SLR on mangrove ecosystems and their vital associated services, to protect and conserve the mangrove ecosystems in the region

Remote Sensing Approach for Monitoring Coastal Wetland in the Mekong Delta, Vietnam: Change Trends and Their Driving Forces

Coastal wetlands in the Mekong Delta (MD), Vietnam, provide various vital ecosystem services for the region. These wetlands have experienced critical changes due to the increase in regional anthropogenic activities, global climate change, and the associated sea level rise (SLR). However, documented information and research on the dynamics and drivers of these important wetland areas remain limited for the region. The present study aims to determine the long-term dynamics of wetlands in the south-west coast of the MD using remote sensing approaches, and analyse the potential factors driving these dynamics. Wetland maps from the years 1995, 2002, 2013, and 2020 at a 15 m spatial resolution were derived from Landsat images with the aid of a hybrid classification approach. The accuracy of the wetland maps was relatively high, with overall accuracies ranging from 86–93%. The findings showed that the critical changes over the period 1995/2020 included the expansion of marine water into coastal lands, showing 129% shoreline erosion" a remarkable increase of 345% in aquaculture ponds" and a reduction of forested wetlands and rice fields/other crops by 32% and 73%, respectively. Although mangrove forests slightly increased for the period 2013/2020, the overall trend was also a reduction of 5%. Our findings show that the substantial increase in aquaculture ponds is at the expense of mangroves, forested wetlands, and rice fields/other crops, while shoreline erosion significantly affected coastal lands, especially mangrove forests. The interaction of a set of environmental and socioeconomic factors were responsible for the dynamics. In particular, SLR was identified as one of the main underlying drivers" however, the rapid changes were directly driven by policies on land-use for economic development in the region. The trends of wetland changes and SLR implicate their significant effects on environment, natural resources, food security, and likelihood of communities in the region sustaining for the long-term. These findings can assist in developing and planning appropriate management strategies and policies for wetland protection and conservation, and for sustainable development in the region

One step closer to understanding the role of bacteria in diabetic foot ulcers: characterising the microbiome of ulcers

Background: The aim of this study was to characterise the microbiome of new and recurrent diabetic foot ulcers using 16S amplicon sequencing (16S AS), allowing the identification of a wider range of bacterial species that may be important in the development of chronicity in these debilitating wounds. Twenty patients not receiving antibiotics for the past three months were selected, with swabs taken from each individual for culture and 16S AS. DNA was isolated using a combination of bead beating and kit extraction. Samples were sequenced on the Illumina Hiseq 2500 platform. Results: Conventional laboratory culture showed positive growth from only 55 % of the patients, whereas 16S AS was positive for 75 % of the patients (41 unique genera, representing 82 different operational taxonomic units (OTU’s). S. aureus was isolated in 72 % of culture-positive samples, whereas the most commonly detected bacteria in all ulcers were Peptoniphilusspp., Anaerococcus spp. and Corynebacterium spp., with the addition of Staphylococcus spp. in new ulcers. The majority of OTU’s residing in both new and recurrent ulcers (over 67 %) were identified as facultative or strict anaerobic Gram-positive organisms. Principal component analysis (PCA) showed no difference in clustering between the two groups (new and recurrent ulcers). Conclusions: The abundance of anaerobic bacteria has important implications for treatment as it suggests that the microbiome of each ulcer “starts afresh” and that, although diverse, are not distinctly different from one another with respect to new or recurrent ulcers. Therefore, when considering antibiotic therapy the duration of current ulceration may be a more important consideration than a history of healed ulcer

Measurement of the cross-section and charge asymmetry of WW bosons produced in proton-proton collisions at s=8\sqrt{s}=8 TeV with the ATLAS detector

This paper presents measurements of the $W^+ \rightarrow \mu^+\nu$ and $W^- \rightarrow \mu^-\nu$ cross-sections and the associated charge asymmetry as a function of the absolute pseudorapidity of the decay muon. The data were collected in proton--proton collisions at a centre-of-mass energy of 8 TeV with the ATLAS experiment at the LHC and correspond to a total integrated luminosity of 20.2~\mbox{fb^{-1}}. The precision of the cross-section measurements varies between 0.8% to 1.5% as a function of the pseudorapidity, excluding the 1.9% uncertainty on the integrated luminosity. The charge asymmetry is measured with an uncertainty between 0.002 and 0.003. The results are compared with predictions based on next-to-next-to-leading-order calculations with various parton distribution functions and have the sensitivity to discriminate between them.Comment: 38 pages in total, author list starting page 22, 5 figures, 4 tables, submitted to EPJC. All figures including auxiliary figures are available at https://atlas.web.cern.ch/Atlas/GROUPS/PHYSICS/PAPERS/STDM-2017-13

Search for chargino-neutralino production with mass splittings near the electroweak scale in three-lepton final states in √s=13 TeV pp collisions with the ATLAS detector

A search for supersymmetry through the pair production of electroweakinos with mass splittings near the electroweak scale and decaying via on-shell W and Z bosons is presented for a three-lepton final state. The analyzed proton-proton collision data taken at a center-of-mass energy of √s=13  TeV were collected between 2015 and 2018 by the ATLAS experiment at the Large Hadron Collider, corresponding to an integrated luminosity of 139  fb−1. A search, emulating the recursive jigsaw reconstruction technique with easily reproducible laboratory-frame variables, is performed. The two excesses observed in the 2015–2016 data recursive jigsaw analysis in the low-mass three-lepton phase space are reproduced. Results with the full data set are in agreement with the Standard Model expectations. They are interpreted to set exclusion limits at the 95% confidence level on simplified models of chargino-neutralino pair production for masses up to 345 GeV

Cancer-initiating cells derived from established cervical cell lines exhibit stem-cell markers and increased radioresistance

<p>Abstract</p> <p>Background</p> <p>Cancer-initiating cells (CICs) are proposed to be responsible for the generation of metastasis and resistance to therapy. Accumulating evidences indicates CICs are found among different human cancers and cell lines derived from them. Few studies address the characteristics of CICs in cervical cancer. We identify biological features of CICs from four of the best-know human cell lines from uterine cervix tumors. (HeLa, SiHa, Ca Ski, C-4 I).</p> <p>Methods</p> <p>Cells were cultured as spheres under stem-cell conditions. Flow cytometry was used to detect expression of CD34, CD49f and CD133 antigens and Hoechst 33342 staining to identify side population (SP). Magnetic and fluorescence-activated cell sorting was applied to enrich and purify populations used to evaluate tumorigenicity in nude mice. cDNA microarray analysis and <it>in vitro </it>radioresistance assay were carried out under standard conditions.</p> <p>Results</p> <p>CICs, enriched as spheroids, were capable to generate reproducible tumor phenotypes in nu-nu mice and serial propagation. Injection of 1 × 10³dissociated spheroid cells induced tumors in the majority of animals, whereas injection of 1 × 10⁵monolayer cells remained nontumorigenic. Sphere-derived CICs expressed CD49f surface marker. Gene profiling analysis of HeLa and SiHa spheroid cells showed up-regulation of CICs markers characteristic of the female reproductive system. Importantly, epithelial to mesenchymal (EMT) transition-associated markers were found highly expressed in spheroid cells. More importantly, gene expression analysis indicated that genes required for radioresistance were also up-regulated, including components of the double-strand break (DSB) DNA repair machinery and the metabolism of reactive oxygen species (ROS). Dose-dependent radiation assay indicated indeed that CICs-enriched populations exhibit an increased resistance to ionizing radiation (IR).</p> <p>Conclusions</p> <p>We characterized a self-renewing subpopulation of CICs found among four well known human cancer-derived cell lines (HeLa, SiHa, Ca Ski and C-4 I) and found that they express characteristic markers of stem cell, EMT and radioresistance. The fact that CICs demonstrated a higher degree of resistance to radiation than differentiated cells suggests that specific detection and targeting of CICs could be highly valuable for the therapy of tumors from the uterine cervix.</p