Bioavailability of iron and zinc in green leafy vegetables growing in river side and local areas of Allahabad district

Introduction: Green Leafy Vegetables (GLVs) are the treasure trove of many micronutrients. Objective: The aim of the study is to find out the commonly growing vegetables in river side and local areas of Allahabad district and to access the bioavailability of iron and zinc in selected green leafy vegetables of river side and local areas of Allahabad district. Methods: Five to four commonly grown green leafy vegetables were selected from the Arailghat, Baluaghat, Gaughat, Mahewa, Muirabad, Rajapur, Rasullabad for the study. Total iron and zinc in sample were estimated by AOAC (2005) and bioavailability of zinc and iron from various food samples was determined in vitro method described by Luten (1996). Appropriate statistical technique was adopted for analysis of study. Result: Soya leaves, Radish leaves, Amaranth, Spinach were grown in both the areas except Kulpha and Karamwa, which are commonly grown in river side area. There was a significant difference between the bioavailability of iron and zinc in GLV grown in local and river side area. Conclusion: Hence it can be concluded that there is a contamination of heavy metals which binds with the iron and zinc and make them less bioavailable in the selected GLV

Sensory and nutritional evaluation of unleavened flat bread prepared by multigrain flour mixture

The present study was undertaken to develop the value added food product using multigrain flour mixture and to assess its sensory and nutritional composition of unleavened flat bread (Chapatti). It was standardized as Control (T0). Along with control; three variations of Chapatti were prepared by replacing wheat flour with different ratio of multigrain flour mixture which referred as T1, T2, T3 and T4 respectively. They were tested for different attrib-utes (Taste and Flavour, Colour and Appearance, Body and Texture and Overall Acceptability). A food composition table given by Gopalan, et.al, 2007 was used to determine the nutritional composition of Chapatti. Appropriate statis-tical technique was opted for the analysis. The result revealed that the T1 (8.05±0.00) was found most acceptable with regards to its sensory attributes followed by T0 (7.70±0.42), T2 (7.55±0.08), T3 (7.22±0.98) and T4 (6.64±0.46) respectively. Energy (ranging from 388-436 Kcal), Protein (ranging from 22-28 g), fat (ranging from 13-21 g), cal-cium (ranging from145-192 mg), phosphorus (ranging from 466-501 mg), fiber (ranging from 3-4g) and iron (ranging from 6-7 mg) were increased in treatments as compared to control except carbohydrate. Thus, it can be concluded that value added product has good organoleptic and nutritional quality

Minimum Dietary Diversity-Women Score, a Key Indicator of Micronutrient Adequacy and its Association with Iron Status among Pregnant Women: Evidence from a Hospital-Based Cross-Sectional Study

A diversified diet is crucial for micronutrient adequacy in a pregnant woman. Iron deficiency Anemia (IDA) is linked to a lack of diversity in diets. The Minimum Dietary Diversity-Women (MDD-W) is a global indicator developed by the FAO to assess diet quality among women of reproductive age. The study seeks to determine the prevalence of MDD scores and its association with sociodemographic factors and iron status among pregnant women. Hospital-based cross-sectional study was conducted among pregnant women at the women’s hospital, Prayagraj, Uttar Pradesh using systematic sampling frame. An enumerator-administered semi-structured interview schedule was used. Hemoglobin levels were assessed and IDA categorized. The prevalence of anemia was 47.4%, of which only 18.1% of women consumed a diverse diet and 81.89% had low MDD-W scores. The mean hemoglobin was 10.82 g/dl, Standard deviation 1.58. Socioeconomic status showed significant correlation to anemia (P = 0.001), and an OR of 1.099 at 95% confidence interval. The high prevalence of IDA and low MDD-W scores proves the need for increased awareness on dietary diversity rates among pregnant women so that micronutrient adequacy can be achieved

Genetics, Treatment, and New Technologies of Hormone Receptor-Positive Breast Cancer

The current molecular classification divides breast cancer into four major subtypes, including luminal A, luminal B, HER2-positive, and basal-like, based on receptor gene expression profiling. Luminal A and luminal B are hormone receptor (HR, estrogen, and/or progesterone receptor)-positive and are the most common subtypes, accounting for around 50–60% and 15–20% of the total breast cancer cases, respectively. The drug treatment for HR-positive breast cancer includes endocrine therapy, HER2-targeted therapy (depending on the HER2 status), and chemotherapy (depending on the risk of recurrence). In this review, in addition to classification, we focused on discussing the important aspects of HR-positive breast cancer, including HR structure and signaling, genetics, including epigenetics and gene mutations, gene expression-based assays, the traditional and new drugs for treatment, and novel or new uses of technology in diagnosis and treatment. Particularly, we have summarized the commonly mutated genes and abnormally methylated genes in HR-positive breast cancer and compared four common gene expression-based assays that are used in breast cancer as prognostic and/or predictive tools in detail, including their clinical use, the factors being evaluated, patient demographics, and the scoring systems. All these topic discussions have not been fully described and summarized within other research or review articles

Multi-omics profiling of the impact of an Angiotensin (1-7)-expressing probiotic combined with exercise training in aged male rats

   Angiotensin (1-7) [Ang (1-7)] is an active heptapeptide of the non-canonical arm of the renin-angiotensin system that modulates molecular signaling pathways associated with vascular and cellular inflammation, vasoconstriction, hyperplasia and fibrosis. Pre-clinical evidence suggests that Ang (1-7) is a promising therapeutic target that may ameliorate physical and cognitive decline in prevalent age-related diseases, but its short half-life and low oral bioavailability reduce its applicability in clinical care. This study evaluated a genetically modified probiotic (GMP) that expresses Ang (1-7) as a potential approach to overcome these barriers. We gave this GMP with and without moderate exercise training and evaluated cross-tissue (prefrontal cortex, hippocampus, colon, liver and skeletal muscle) multi-omics responses in aged male rats. After 12-weeks of intervention, the GMP and exercise training distinctly altered fecal microbiome composition. The GMP altered 3 generas while exercise training enhanced beta-diversity. Exercise training also significantly altered neuro-remodeling, inflammation and circadian rhythm gene expression on relevant regulatory tissues. </p