526 research outputs found
Genetic Studies of Sulfadiazine-resistant and Methionine-requiring \u3cem\u3eNeisseria\u3c/em\u3e Isolated From Clinical Material
Deoxyribonucleate (DNA) preparations were extracted from Neisseria meningitidis (four isolates from spinal fluid and blood) and N. gonorrhoeae strains, all of which were resistant to sulfadiazine upon primary isolation. These DNA preparations, together with others from in vitro mutants of N. meningitidis and N. perflava, were examined in transformation tests by using as recipient a drug-susceptible strain of N. meningitidis (Ne 15 Sul-s Met+) which was able to grow in a methionine-free defined medium. The sulfadiazine resistance typical of each donor was introduced into the uniform constitution of this recipient. Production of p-aminobenzoic acid was not significantly altered thereby. Transformants elicited by DNA from the N. meningitidis clinical isolates were resistant to at least 200 μg of sulfadiazine/ml, and did not show a requirement for methionine (Sul-r Met+). DNA from six strains of N. gonorrhoeae, which were isolated during the period of therapeutic use of sulfonamides, conveyed lower degrees of resistance and, invariably, a concurrent methionine requirement (Sul-r/Met−). The requirement of these transformants, and that of in vitro mutants selected on sulfadiazine-agar, was satisfied by methionine, but not by vitamin B12, homocysteine, cystathionine, homoserine, or cysteine. Sul-r Met+ and Sul-r/Met− loci could coexist in the same genome, but were segregated during transformation. On the other hand, the dual Sul-r/Met− properties were not separated by recombination, but were eliminated together. DNA from various Sul-r/Met− clones tested against recipients having nonidentical Sul-r/Met− mutant sites yielded Sul-s Met+ transformants. The met locus involved is genetically complex, and will be a valuable tool for studies of genetic fine structure of members of Neisseria, and of genetic homology between species
A dynamical classification of the range of pair interactions
We formalize a classification of pair interactions based on the convergence
properties of the {\it forces} acting on particles as a function of system
size. We do so by considering the behavior of the probability distribution
function (PDF) P(F) of the force field F in a particle distribution in the
limit that the size of the system is taken to infinity at constant particle
density, i.e., in the "usual" thermodynamic limit. For a pair interaction
potential V(r) with V(r) \rightarrow \infty) \sim 1/r^a defining a {\it
bounded} pair force, we show that P(F) converges continuously to a well-defined
and rapidly decreasing PDF if and only if the {\it pair force} is absolutely
integrable, i.e., for a > d-1, where d is the spatial dimension. We refer to
this case as {\it dynamically short-range}, because the dominant contribution
to the force on a typical particle in this limit arises from particles in a
finite neighborhood around it. For the {\it dynamically long-range} case, i.e.,
a \leq d-1, on the other hand, the dominant contribution to the force comes
from the mean field due to the bulk, which becomes undefined in this limit. We
discuss also how, for a \leq d-1 (and notably, for the case of gravity, a=d-2)
P(F) may, in some cases, be defined in a weaker sense. This involves a
regularization of the force summation which is generalization of the procedure
employed to define gravitational forces in an infinite static homogeneous
universe. We explain that the relevant classification in this context is,
however, that which divides pair forces with a > d-2 (or a < d-2), for which
the PDF of the {\it difference in forces} is defined (or not defined) in the
infinite system limit, without any regularization. In the former case dynamics
can, as for the (marginal) case of gravity, be defined consistently in an
infinite uniform system.Comment: 12 pages, 1 figure; significantly shortened and focussed, additional
references, version to appear in J. Stat. Phy
72. Incision en « V » axillaire dans la prise en charge des tumeurs du quadrant supéro-externe et du prolongement axillaire du sein
Microsystem Technology for Ambient Assisted Living (AAL)
AbstractAAL is certainly an application area with sensor as well as actuator needs. Some of the requirements can be fulfilled by state of the art technology; some areas however still need a lot of R&D efforts for potential applications in homes. The contribution describes two areas of interest and actual development: One is the topic of robust fire detection; the other domain is fall detection. For both application areas one has to understand both the state of the art and the drawbacks of the current solutions. One can state clearly that there is a huge potential for the development of new microsystems. Still one has to keep in mind that usage in elderly homes also requires consent and cooperation of the users which is the focus of the user centered design principle
Flow cytometry based techniques to study testicular acidophilic granulocytes from the protandrous fish gilthead seabream (Sparus aurata L.)
The gilthead seabream is a protandrous seasonal breeding teleost that is an excellent model for studying the testicular regression process which occurs in both seasonal testicular involution and sex reversion. Little is known about the cell types and the molecular mechanisms involved in such processes, mainly because of the lack of appropriate methods for testis dissociation, and testicular cell isolation, culture and functional characterization. We have previously reported that gilthead seabream acidophilic granulocytes infiltrate the testis at post-spawning stage, settle close to the spermatogonia and accumulate intracellular interleukin-1β. In this paper, we report several flow cytometry based assays which allow to establish the role played by gilthead seabream testicular acidophilic granulocytes and permits their quantification
Animal models of focal brain ischemia
Stroke is a leading cause of disability and death in many countries. Understanding the pathophysiology of ischemic injury and developing therapies is an important endeavor that requires much additional research. Animal stroke models provide an important mechanism for these activities. A large number of stroke models have been developed and are currently used in laboratories around the world. These models are overviewed as are approaches for measuring infarct size and functional outcome
Imaging Jupiter's radiation belts down to 127 MHz with LOFAR
Context. Observing Jupiter's synchrotron emission from the Earth remains
today the sole method to scrutinize the distribution and dynamical behavior of
the ultra energetic electrons magnetically trapped around the planet (because
in-situ particle data are limited in the inner magnetosphere). Aims. We perform
the first resolved and low-frequency imaging of the synchrotron emission with
LOFAR at 127 MHz. The radiation comes from low energy electrons (~1-30 MeV)
which map a broad region of Jupiter's inner magnetosphere. Methods (see article
for complete abstract) Results. The first resolved images of Jupiter's
radiation belts at 127-172 MHz are obtained along with total integrated flux
densities. They are compared with previous observations at higher frequencies
and show a larger extent of the synchrotron emission source (>=4 ). The
asymmetry and the dynamic of east-west emission peaks are measured and the
presence of a hot spot at lambda_III=230 {\deg} 25 {\deg}. Spectral flux
density measurements are on the low side of previous (unresolved) ones,
suggesting a low-frequency turnover and/or time variations of the emission
spectrum. Conclusions. LOFAR is a powerful and flexible planetary imager. The
observations at 127 MHz depict an extended emission up to ~4-5 planetary radii.
The similarities with high frequency results reinforce the conclusion that: i)
the magnetic field morphology primarily shapes the brightness distribution of
the emission and ii) the radiating electrons are likely radially and
latitudinally distributed inside about 2 . Nonetheless, the larger extent
of the brightness combined with the overall lower flux density, yields new
information on Jupiter's electron distribution, that may shed light on the
origin and mode of transport of these particles.Comment: 10 pages, 12 figures, accepted for publication in A&A (27/11/2015) -
abstract edited because of limited character
Four-dimensional distribution of the 2010 Eyjafjallajökull volcanic cloud over Europe observed by EARLINET
© Author(s) 2013. This work is distributed under the Creative Commons Attribution 3.0 License.The eruption of the Icelandic volcano Eyjafjallaj ökull in April-May 2010 represents a "natural experiment" to study the impact of volcanic emissions on a continental scale. For the first time, quantitative data about the presence, altitude, and layering of the volcanic cloud, in conjunction with optical information, are available for most parts of Europe derived from the observations by the European Aerosol Research Lidar NETwork (EARLINET). Based on multi-wavelength Raman lidar systems, EARLINET is the only instrument worldwide that is able to provide dense time series of high-quality optical data to be used for aerosol typing and for the retrieval of particle microphysical properties as a function of altitude. In this work we show the four-dimensional (4-D) distribution of the Eyjafjallajökull volcanic cloud in the troposphere over Europe as observed by EARLINET during the entire volcanic event (15 April-26 May 2010). All optical properties directly measured (backscatter, extinction, and particle linear depolarization ratio) are stored in the EARLINET database available at www.earlinet.org. A specific relational database providing the volcanic mask over Europe, realized ad hoc for this specific event, has been developed and is available on request at www.earlinet.org. During the first days after the eruption, volcanic particles were detected over Central Europe within a wide range of altitudes, from the upper troposphere down to the local planetary boundary layer (PBL). After 19 April 2010, volcanic particles were detected over southern and south-eastern Europe. During the first half of May (5-15 May), material emitted by the Eyjafjallajökull volcano was detected over Spain and Portugal and then over the Mediterranean and the Balkans. The last observations of the event were recorded until 25 May in Central Europe and in the Eastern Mediterranean area. The 4-D distribution of volcanic aerosol layering and optical properties on European scale reported here provides an unprecedented data set for evaluating satellite data and aerosol dispersion models for this kind of volcanic events.Peer reviewe
A systematic review of physiological methods in rodent pharmacological MRI studies
Rationale: Pharmacological magnetic resonance imaging (phMRI) provides an approach to study effects of drug challenges on brain processes. Elucidating mechanisms of drug action helps us to better understand the workings of neurotransmitter systems, map brain function or facilitate drug development. phMRI is increasingly used in preclinical research employing rodent models; however, data interpretation and integration are complicated by the use of different experimental approaches between laboratories. In particular, the effects of different anaesthetic regimes upon neuronal and haemodynamic processes and baseline physiology could be problematic.
Objectives: This paper investigates how differences in phMRI research methodologies are manifested and considers associated implications, placing particular emphasis on choice of anaesthetic regimes.
Methods: A systematic review of rodent phMRI studies was conducted. Factors such as those describing anaesthetic regimes (e.g. agent, dosage) and parameters relating to physiological maintenance (e.g. ventilatory gases) and MRI method were recorded.
Results: We identified 126 eligible studies and found that the volatile agents isoflurane (43.7 %) and halothane (33.3 %) were most commonly used for anaesthesia, but dosage and mixture of ventilatory gases varied substantially between laboratories. Relevant physiological parameters were usually recorded, although 32 % of studies did not provide cardiovascular measures.
Conclusions: Anaesthesia and animal preparation can influence phMRI data profoundly. The variation of anaesthetic type, dosage regime and ventilatory gases makes consolidation of research findings (e.g. within a specific neurotransmitter system) difficult. Standardisation of a small(er) number of preclinical phMRI research methodologies and/or increased consideration of approaches that do not require anaesthesia is necessary to address these challenges
Sleep deprivation impairs and caffeine enhances my performance, but not always our performance: how acting in a group can change the effects of impairments and enhancements
What effects do factors that impair or enhance performance in individuals have when these individuals act in groups? We provide a framework, called the GIE ("Effects of Grouping on Impairments and Enhancements”) framework, for investigating this question. As prominent examples for individual-level impairments and enhancements, we discuss sleep deprivation and caffeine. Based on previous research, we derive hypotheses on how they influence performance in groups, specifically process gains and losses in motivation, individual capability, and coordination. We conclude that the effect an impairment or enhancement has on individual-level performance is not necessarily mirrored in group performance: grouping can help or hurt. We provide recommendations on how to estimate empirically the effects individual-level performance impairments and enhancements have in groups. By comparing sleep deprivation to stress and caffeine to pharmacological cognitive enhancement, we illustrate that we cannot readily generalize from group results on one impairment or enhancement to another, even if they have similar effects on individual-level performance
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