Plug-and-Participate for Limited Devices in the Field of Industrial Automation

Ausgangspunkt und gleichzeitig Motivation dieser Arbeit ist die heutige Marktsituation: Starke Kundenbedürfnisse nach individuellen Gütern stehen oftmals eher auf Massenproduktion ausgerichteten Planungs- und Automatisierungssystemen gegenüber - die Befriedigung individueller Kundenbedürfnisse setzt aber Flexibilität und Anpassungsfähigkeit voraus. Ziel dieser Arbeit ist es daher, einen Beitrag zu leisten, der es Unternehmen ermöglichen soll, auf diese individuellen Bedürfnisse flexibel reagieren zu können. Hierbei kann es im Rahmen der Dissertation natürlich nicht um eine Revolutionierung der gesamten Automatisierungs- und Planungslandschaft gehen; vielmehr ist die Lösung, die der Autor der Arbeit präsentiert, ein integraler Bestandteil eines Automatisierungskonzeptes, das im Rahmen des PABADIS Projektes entwickelt wurde: Während PABADIS das gesamte Spektrum von Planung und Maschineninfrastruktur zum Inhalt hat, bezieht sich der Kern dieser Arbeit weitestgehend auf den letztgenannten Punkt - Maschineninfrastruktur. Ziel war es, generische Maschinenfunktionalität in einem Netzwerk anzubieten, durch das Fertigungsaufträge selbstständig navigieren. Als Lösung präsentiert diese Dissertation ein Plug-and-Participate basiertes Konzept, welches beliebige Automatisierungsfunktionen in einer spontanen Gemeinschaft bereitstellt. Basis ist ein generisches Interface, in dem die generellen Anforderungen solcher ad-hoc Infrastrukturen aggregiert sind. Die Implementierung dieses Interfaces in der PABADIS Referenzimplementierung sowie die Gegenüberstellung der Systemanforderungen und Systemvoraussetzungen zeigte, das klassische Plug-and-Participate Technologien wie Jini und UPnP aufgrund ihrer Anforderungen nicht geeignet sind - Automatisierungsgeräte stellen oftmals nur eingeschränkte Ressourcen bereit. Daher wurde als zweites Ergebnis neben dem Plug-and-Participate basierten Automatisierungskonzept eine Plug-and-Participate Technologie entwickelt - Pini - die den Gegebenheiten der Automatisierungswelt gerecht wird und schließlich eine Anwendung von PABADIS auf heutigen Automatisierungsanlagen erlaubt. Grundlegende Konzepte von Pini, die dies ermöglichen, sind die gesamte Grundarchitektur auf Basis eines verteilten Lookup Service, die Art und Weise der Dienstrepräsentation sowie die effiziente Nutzung der angebotenen Dienste. Mit Pini und darauf aufbauenden Konzepten wie PLAP ist es nun insbesondere möglich, Automatisierungssysteme wie PABADIS auf heutigen Anlagen zu realisieren. Das wiederum ist ein Schritt in Richtung Kundenorientierung - solche Systeme sind mit Hinblick auf Flexibilität und Anpassungsfähigkeit gestaltet worden, um Kundenbedürfnissen effizient gerecht zu werden

Subglobale Allianzen in der internationalen Klimapolitik

Die Vermeidung eines ungebremsten Klimawandels bedarf universeller Kooperation. Das gegebene multilaterale Klimaregime leistet dies gegenwärtig nur unzureichend. Subglobale Allianzen ambitionierter Klimapioniere könnten den unhaltbaren Status quo der internationalen Klimapolitik überwinden

Ausprägungen und Nutzungsgrad der Logistiksimulation im Umfeld der Automobilindustrie

In Wissenschaft und Praxis wird die Materialflusssimulation für eine Vielzahl von Anwendungsszenarien in Produktion und Logistik verwendet. Fokus dieses Beitrags sind die unter dem Oberbegriff „Logistiksimulation“ im automobilen Umfeld zusammengefassten Ausprägungen dieser Simulationsmethode. Diese werden von den einzelnen Automobilherstellern in unterschiedlichen Detaillierungsgraden und unterschiedlichen Bandbreiten eingesetzt. Bisher konnten sich allerdings keine gemeinsamen Bezeichnungen für diese Ausprägungen der Logistiksimulation durchsetzen. Um einen einheitlichen Standard im Umfeld der automobilen Logistik zu definieren, wurde eine Umfrage bei den deutschen Automobilherstellern durchgeführt. Das Ergebnis zeigt, dass aktuell vier Ausprägungen der Simulation im Bereich der Automotive-Logistik eingesetzt werden, die auf Grund ihrer Fragestellungen sehr unterschiedlich zu behandeln sind. Dies sind die Werkssimulation, die Belieferungssimulation, die Supply-Chain-Simulation und die Verkehrsflusssimulation. Durch die übergreifende Definition der verwendeten Simulationen, Festlegung der Betrachtungsumfänge und Systemgrenzen sowie die Rolle im Produktentstehungsprozess wird eine Vergleichbarkeit geschaffen. Auf der Basis dieser Standardisierung können Zusammenarbeitsmodelle vereinbart und gemeinsame Forschungsvorhaben angestoßen werden

Constrained simulations of the Local Group: on the radial distribution of substructures

We examine the properties of satellites found in high resolution simulations of the local group. We use constrained simulations designed to reproduce the main dynamical features that characterize the local neighborhood, i.e. within tens of Mpc around the Local Group (LG). Specifically, a LG-like object is found located within the 'correct' dynamical environment and consisting of three main objects which are associated with the Milky Way, M31 and M33. By running two simulations of this LG from identical initial conditions - one with and one without baryons modeled hydrodynamically - we can quantify the effect of gas physics on the $z=0$ population of subhaloes in an environment similar to our own. We find that above a certain mass cut, $M_{\rm sub} > 2\times10^{8}h^{-1} M_{\odot}$ subhaloes in hydrodynamic simulations are more radially concentrated than those in simulations with out gas. This is caused by the collapse of baryons into stars that typically sit in the central regions of subhaloes, making them denser. The increased central density of such a subhalo, results in less mass loss due to tidal stripping than the same subhalo simulated with only dark matter. The increased mass in hydrodynamic subhaloes with respect to dark matter ones, causes dynamical friction to be more effective, dragging the subhalo towards the centre of the host. This results in these subhaloes being effectively more radially concentrated then their dark matter counterparts.Comment: 12 pages, 9 figure

Constraining warm dark matter with cosmic shear power spectra

We investigate potential constraints from cosmic shear on the dark matter particle mass, assuming all dark matter is made up of light thermal relic particles. Given the theoretical uncertainties involved in making cosmological predictions in such warm dark matter scenarios we use analytical fits to linear warm dark matter power spectra and compare (i) the halo model using a mass function evaluated from these linear power spectra and (ii) an analytical fit to the non-linear evolution of the linear power spectra. We optimistically ignore the competing effect of baryons for this work. We find approach (ii) to be conservative compared to approach (i). We evaluate cosmological constraints using these methods, marginalising over four other cosmological parameters. Using the more conservative method we find that a Euclid-like weak lensing survey together with constraints from the Planck cosmic microwave background mission primary anisotropies could achieve a lower limit on the particle mass of 2.5 keV.Comment: 26 pages, 9 figures, minor changes to match the version accepted for publication in JCA

Cell-based non-invasive prenatal testing for monogenic disorders:confirmation of unaffected fetuses following preimplantation genetic testing

PURPOSE: Proof of concept of the use of cell-based non-invasive prenatal testing (cbNIPT) as an alternative to chorionic villus sampling (CVS) following preimplantation genetic testing for monogenic disorders (PGT-M). METHOD: PGT-M was performed by combined testing of short tandem repeat (STR) markers and direct mutation detection, followed by transfer of an unaffected embryo. Patients who opted for follow-up of PGT-M by CVS had blood sampled, from which potential fetal extravillous throphoblast cells were isolated. The cell origin and mutational status were determined by combined testing of STR markers and direct mutation detection using the same setup as during PGT. The cbNIPT results with respect to the mutational status were compared to those of genetic testing of the CVS. RESULTS: Eight patients had blood collected between gestational weeks 10 and 13, from which 33 potential fetal cell samples were isolated. Twenty-seven out of 33 isolated cell samples were successfully tested (82%), of which 24 were of fetal origin (89%). This corresponds to a median of 2.5 successfully tested fetal cell samples per case (range 1–6). All fetal cell samples had a genetic profile identical to that of the transferred embryo confirming a pregnancy with an unaffected fetus, in accordance with the CVS results. CONCLUSION: These findings show that although measures are needed to enhance the test success rate and the number of cells identified, cbNIPT is a promising alternative to CVS. TRIAL REGISTRATION NUMBER: N-20180001 SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10815-021-02104-5

No effects of GSM-modulated 900 MHz electromagnetic fields on survival rate and spontaneous development of lymphoma in female AKR/J mice

BACKGROUND: Several reports indicated that non-thermal electromagnetic radiation such as from mobile phones and base stations may promote cancer. Therefore, it was investigated experimentally, whether 900 MHz electromagnetic field exposure influences lymphoma development in a mouse strain that is genetically predisposed to this disease. The AKR/J mice genome carries the AK-virus, which leads within one year to spontaneous development of thymic lymphoblastic lymphoma. METHODS: 320 unrestrained female mice were sham-exposed or exposed (each n = 160 animals) to GSM like 900 MHz electromagnetic fields for 24 hours per day, 7 days per week, at an average whole body specific absorption rate (SAR) value of 0.4 W/kg. Animals were visually checked daily and were weighed and palpated weekly. Starting with an age of 6 months, blood samples were taken monthly from the tail. Animals with signs of disease or with an age of about 46 weeks were sacrificed and a gross necropsy was performed. RESULTS: Electromagnetic field exposure had a significant effect on body weight gain, with higher values in exposed than in sham-exposed animals. However, survival rate and lymphoma incidence did not differ between exposed and sham-exposed mice. CONCLUSION: These data do not support the hypothesis that exposure to 900 MHz electromagnetic fields is a significant risk factor for developing lymphoma in a genetically predisposed species, even at a relatively high exposure level

Author response table 1.

Gut bacteria occupy the interface between the organism and the external environment, contributing to homeostasis and disease. Yet, the causal role of the gut microbiota during host aging is largely unexplored. Here, using the African turquoise killifish (Nothobranchius furzeri), a naturally short-lived vertebrate, we show that the gut microbiota plays a key role in modulating vertebrate life span. Recolonizing the gut of middle-age individuals with bacteria from young donors resulted in life span extension and delayed behavioral decline. This intervention prevented the decrease in microbial diversity associated with host aging and maintained a young-like gut bacterial community, characterized by overrepresentation of the key genera Exiguobacterium, Planococcus, Propionigenium and Psychrobacter. Our findings demonstrate that the natural microbial gut community of young individuals can causally induce long-lasting beneficial systemic effects that lead to life span extension in a vertebrate model

Next-generation sequencing (NGS) for assessment of microbial water quality: current progress, challenges, and future opportunities

Water quality is an emergent property of a complex system comprised of interacting microbial populations and introduced microbial and chemical contaminants. Studies leveraging next-generation sequencing (NGS) technologies are providing new insights into the ecology of microbially mediated processes that influence fresh water quality such as algal blooms, contaminant biodegradation, and pathogen dissemination. In addition, sequencing methods targeting small subunit (SSU) rRNA hypervariable regions have allowed identification of signature microbial species that serve as bioindicators for sewage contamination in these environments. Beyond amplicon sequencing, metagenomic and metatranscriptomic analyses of microbial communities in fresh water environments reveal the genetic capabilities and interplay of waterborne microorganisms, shedding light on the mechanisms for production and biodegradation of toxins and other contaminants. This review discusses the challenges and benefits of applying NGS-based methods to water quality research and assessment. We will consider the suitability and biases inherent in the application of NGS as a screening tool for assessment of biological risks and discuss the potential and limitations for direct quantitative interpretation of NGS data. Secondly, we will examine case studies from recent literature where NGS based methods have been applied to topics in water quality assessment, including development of bioindicators for sewage pollution and microbial source tracking, characterizing the distribution of toxin and antibiotic resistance genes in water samples, and investigating mechanisms of biodegradation of harmful pollutants that threaten water quality. Finally, we provide a short review of emerging NGS platforms and their potential applications to the next generation of water quality assessment tools.Singapore-MIT Alliance for Research and Technology. Center for Environmental Sensing and Modelin