Optimization of pathogenicity assays to study the Arabidopsis thaliana-Xanthomonas campestris pv. campastris pathosystem

The cruciferous weed Arabidopsis thaliana and the causal agent of black rot disease of Crucifers Xanthomonas campestris pv. campestris ( Xcc ) are both model organisms in plant pathology. Their interaction has been studied successfully in the past, but these investigations suffered from high variability. In the present study, we describe an improved Arabidopsis- Xcc pathosystem that is based on a wound inoculation procedure. We show that after wound inoculation, Xcc colonizes the vascular system of Arabidopsis leaves and causes typical black rot symptoms in a compatible interaction, while in an incompatible interaction bacterial multiplication is inhibited. The highly synchronous and reproducible symptom expression allowed the development of a disease scoring scheme that enabled us to analyse the effects of mutations in individual genes on plant resistance or on bacterial virulence in a simple and precise manner. This optimized Arabidopsis- Xcc pathosystem will be a robust tool for further genetic and post-genomic investigation of fundamental questions in plant pathology. (Résumé d'auteur

High redshift evolution of optically and IR-selected galaxies: a comparison with CDM scenarios

A combination of ground-based (NTT and VLT) and HST (HDF-N and HDF-S) public imaging surveys have been used to collect a sample of 1712 I-selected and 319 $K\leq 21$ galaxies. Photometric redshifts have been obtained for all these galaxies. The results have been compared with the prediction of an analytic rendition of the current CDM hierarchical models for galaxy formation. We focus in particular on two observed quantities: the galaxy redshift distribution at K<21 and the evolution of the UV luminosity density. The derived photometric redshift distribution is in agreement with the hierarchical CDM prediction, with a fraction of only 5% of galaxies detected at z>2. This result strongly supports hierarchical scenarios where present-day massive galaxies are the result of merging processes. The observed UV luminosity density in the I-selected sample is confined within a factor of 4 over the whole range 0<z<4.5. CDM models in a critical Universe are not able to produce the density of UV photons that is observed at z>3. CDM models in $\Lambda$-dominated universe are in better agreement at 3<z<4.5, but predict a pronounced peak at z~1.5 and a drop by a factor of 8 from z=1.5 to z=4 that is not observed in the data. We conclude that improvements are required in the treatment of the physical processes directly related to the SFR, e.g. the starbust activity in merger processes and/or different feedback to the star formation activity.Comment: Figures 2 and 3 modified to match the published versio

Exploring access to end of life care for ethnic minorities with end stage kidney disease through recruitment in action research

BACKGROUND: Variation in provision of palliative care in kidney services and practitioner concerns to provide equitable access led to the development of this study which focussed on the perspectives of South Asian patients and their care providers. As people with a South Asian background experience a higher risk of Type 2 Diabetes (T2DM) and end stage kidney failure (ESKF) compared to the majority population but wait longer for a transplant, there is a need for end of life care to be accessible for this group of patients. Furthermore because non English speakers and people at end of life are often excluded from research there is a dearth of research evidence with which to inform service improvement. This paper aims to explore issues relating to the process of recruitment of patients for a research project which contribute to our understanding of access to end of life care for ethnic minority patients in the kidney setting. METHODS: The study employed an action research methodology with interviews and focus groups to capture and reflect on the process of engaging with South Asian patients about end of life care. Researchers and kidney care clinicians on four NHS sites in the UK recruited South Asian patients with ESKF who were requiring end of life care to take part in individual interviews; and other clinicians who provided care to South Asian kidney patients at end of life to take part in focus groups exploring end of life care issues. In action research planning, action and evaluation are interlinked and data were analysed with emergent themes fed back to care providers through the research cycle. Reflections on the process of patient recruitment generated focus group discussions about access which were analysed thematically and reported here. RESULTS: Sixteen patients were recruited to interview and 45 different care providers took part in 14 focus groups across the sites. The process of recruiting patients to interview and subsequent focus group data highlighted some of the key issues concerning access to end of life care. These were: the identification of patients approaching end of life; and their awareness of end of life care; language barriers and informal carers' roles in mediating communication; and contrasting cultures in end of life kidney care. CONCLUSIONS: Reflection on the process of recruitment in this action research study provided insight into the complex scenario of end of life in kidney care. Some of the emerging issues such as the difficulty identifying patients are likely to be common across all patient groups, whilst others concerning language barriers and third party communication are more specific to ethnic minorities. A focus on South Asian ethnicity contributes to better understanding of patient perspectives and generic concepts as well as access to end of life kidney care for this group of patients in the UK. Action research was a useful methodology for achieving this and for informing future research to include informal carers and other ethnic groups.Peer reviewedFinal Published versio

Semi-Analytic Modelling of Galaxy Formation: The Local Universe

Using semi-analytic models of galaxy formation, we investigate galaxy properties such as the Tully-Fisher relation, the B and K-band luminosity functions, cold gas contents, sizes, metallicities, and colours, and compare our results with observations of local galaxies. We investigate several different recipes for star formation and supernova feedback, including choices that are similar to the treatment in Kauffmann, White & Guiderdoni (1993) and Cole et al. (1994) as well as some new recipes. We obtain good agreement with all of the key local observations mentioned above. In particular, in our best models, we simultaneously produce good agreement with both the observed B and K-band luminosity functions and the I-band Tully-Fisher relation. Improved cooling and supernova feedback modelling, inclusion of dust extinction, and an improved Press-Schechter model all contribute to this success. We present results for several variants of the CDM family of cosmologies, and find that models with values of $\Omega_0 \simeq 0.3$--0.5 give the best agreement with observations.Comment: 26 pages, LaTeX, MNRAS format, 23 inlined postscript figures. Accepted for publication in MNRAS. Revised version contains substantial changes including improved models. High resolution figures, original version, and summary of changes may be found at http://www.fiz.huji.ac.il/~rachels/papers/sp.htm

On merger bias and the clustering of quasars

We use the large catalogues of haloes available for the Millennium Simulation to test whether recently merged haloes exhibit stronger large-scale clustering than other haloes of the same mass. This effect could help to understand the very strong clustering of quasars at high redshift. However, we find no statistically significant excess bias for recently merged haloes over the redshift range 2 < z < 5, with the most massive haloes showing an excess of at most ~5%. We also consider galaxies extracted from a semianalytic model built on the Millennium Simulation. At fixed stellar mass, we find an excess bias of ~ 20-30% for recently merged objects, decreasing with increasing stellar mass. The fact that recently-merged galaxies are found in systematically more massive haloes than other galaxies of the same stellar mass accounts for about half of this signal, and perhaps more for high-mass galaxies. The weak merger bias of massive systems suggests that objects of merger-driven nature, such as quasars, do not cluster significantly differently than other objects of the same characteristic mass. We discuss the implications of these results for the interpretation of clustering data with respect to quasar duty cycles, visibility times, and evolution in the black hole-host mass relation.Comment: 10 pages, 9 figures. Submitted to MNRAS. Comments welcom

Semi-analytic modeling of the EBL and consequences for extragalactic gamma-ray spectra

Attenuation of high-energy gamma rays by pair-production with UV, optical and IR extragalactic background light (EBL) photons provides a link between the history of galaxy formation and high-energy astrophysics. We present results from our latest semi-analytic models (SAMs), which employ the main ingredients thought to be important to galaxy formation and evolution, as well as an improved model for reprocessing of starlight by dust to mid- and far-IR wavelengths. These SAMs are based upon a Lambda-CDM hierarchical structural formation scenario, and are successful in reproducing a large variety of observational constraints such as number counts, luminosity and mass functions, and color bimodality. Our fiducial model is based upon a WMAP5 cosmology, and treats dust emission using empirical templates. This model predicts a background flux considerably lower than optical and near-IR measurements that rely on subtraction of zodiacal and galactic foregrounds, and near the lower bounds set by number counts of resolvable sources at a large number of wavelengths. We also show the results of varying cosmological parameters and dust attenuation model used in our SAM. For each EBL prediction, we show how the optical depth due to electron-positron pair-production is affected by redshift and gamma-ray energy, and the effect of gamma-ray absorption on the spectra of a variety of extragalactic sources. We conclude with a discussion of the implications of our work, comparisons to other models and key measurements of the EBL and a discussion of how the burgeoning science of gamma-ray astronomy will continue to help constrain cosmology. The low EBL flux predicted by our fiducial model suggests an optimistic future for further studies of distant gamma-ray sources.Comment: 23 pages, 11 figures, 3 tables, accepted by MNRAS; this preprint matches accepted versio

TGF-b2 induction regulates invasiveness of theileria-transformed leukocytes and disease susceptibility

Theileria parasites invade and transform bovine leukocytes causing either East Coast fever (T. parva), or tropical theileriosis (T. annulata). Susceptible animals usually die within weeks of infection, but indigenous infected cattle show markedly reduced pathology, suggesting that host genetic factors may cause disease susceptibility. Attenuated live vaccines are widely used to control tropical theileriosis and attenuation is associated with reduced invasiveness of infected macrophages in vitro. Disease pathogenesis is therefore linked to aggressive invasiveness, rather than uncontrolled proliferation of Theileria-infected leukocytes. We show that the invasive potential of Theileria-transformed leukocytes involves TGF-b signalling. Attenuated live vaccine lines express reduced TGF-b2 and their invasiveness can be rescued with exogenous TGF-b. Importantly, infected macrophages from disease susceptible Holstein-Friesian (HF) cows express more TGF-b2 and traverse Matrigel with great efficiency compared to those from disease-resistant Sahiwal cattle. Thus, TGF-b2 levels correlate with disease susceptibility. Using fluorescence and time-lapse video microscopy we show that Theileria-infected, disease-susceptible HF macrophages exhibit increased actin dynamics in their lamellipodia and podosomal adhesion structures and develop more membrane blebs. TGF-b2-associated invasiveness in HF macrophages has a transcription-independent element that relies on cytoskeleton remodelling via activation of Rho kinase (ROCK). We propose that a TGF-b autocrine loop confers an amoeboid-like motility on Theileria-infected leukocytes, which combines with MMP-dependent motility to drive invasiveness and virulence

Stellar populations of early-type galaxies in different environments I. Line-strength indices

Aims: This paper commences a series devoted to the study of the stellar content of early-type galaxies. The goal of the series is to set constraints on the evolutionary status of these objects. Methods: In this paper we describe the details of the galaxy sample, the observations, and the data reduction. Line-strength indices and velocity dispersions sigma are measured in 98 early-type galaxies drawn from different environments, and the relation of the indices with the velocity dispersion analysed in detail. Results: The present sample indicates that some of the index-sigma relations depend on galaxy environment. In particular, the slope of the relation between Balmer lines and sigma is steeper for galaxies in the Virgo cluster, small groups, and in the field than for galaxies in the Coma cluster. In several indices there is also a significant offset in the zero point between the relations defined by the different subsamples. The slopes of the index-sigma relation for the Virgo and low-density environment galaxies are explained by a variation of both age and metallicity with velocity dispersion, as previously noted in other studies. For the galaxies in the Coma cluster, however, the relation of the indices with sigma only requires a variation of the abundance along the sigma sequence. In agreement with other studies we find that the models that better reproduce the slopes are those in which the alpha elements vary more than the Fe-peak elements along the sigma sequence, while, at a given sigma, older galaxies show an higher alpha/Fe ratio. Conclusions: The results can be explained assuming that galaxies in the Coma cluster have experienced a truncated star formation and chemical enrichment history compared to a more continuous time-extended history for their counterparts in lower density environments.Comment: accepted for publication in A&

Fitting the integrated Spectral Energy Distributions of Galaxies

Fitting the spectral energy distributions (SEDs) of galaxies is an almost universally used technique that has matured significantly in the last decade. Model predictions and fitting procedures have improved significantly over this time, attempting to keep up with the vastly increased volume and quality of available data. We review here the field of SED fitting, describing the modelling of ultraviolet to infrared galaxy SEDs, the creation of multiwavelength data sets, and the methods used to fit model SEDs to observed galaxy data sets. We touch upon the achievements and challenges in the major ingredients of SED fitting, with a special emphasis on describing the interplay between the quality of the available data, the quality of the available models, and the best fitting technique to use in order to obtain a realistic measurement as well as realistic uncertainties. We conclude that SED fitting can be used effectively to derive a range of physical properties of galaxies, such as redshift, stellar masses, star formation rates, dust masses, and metallicities, with care taken not to over-interpret the available data. Yet there still exist many issues such as estimating the age of the oldest stars in a galaxy, finer details ofdust properties and dust-star geometry, and the influences of poorly understood, luminous stellar types and phases. The challenge for the coming years will be to improve both the models and the observational data sets to resolve these uncertainties. The present review will be made available on an interactive, moderated web page (sedfitting.org), where the community can access and change the text. The intention is to expand the text and keep it up to date over the coming years.Comment: 54 pages, 26 figures, Accepted for publication in Astrophysics & Space Scienc

Relating Differently to Intrusive Images: The Impact of Mindfulness Based Cognitive Therapy (MBCT) on Intrusive Images in Patients With Severe Health Anxiety (Hypochondriasis).

Recurrent distressing intrusive images are a common experience in hypochondriasis. The aim of the current study was to assess the impact of Mindfulness-Based Cognitive Therapy (MBCT) for hypochondriasis on the occurrence and nature of distressing intrusive imagery in hypochondriasis. A semi-structured interview was used to assess intrusive imagery, and an adapted version of the Southampton Mindfulness Questionnaire (SMQ) was used to assess participants’ relationship with their intrusive images. A consecutive series of participants (N = 20) who were receiving MBCT for hypochondriasis as part of an ongoing research program were assessed prior to participating in an 8-week MBCT intervention, immediately following the intervention, and at three month follow-up. As compared to the baseline assessment, the frequency of intrusive images, the distress associated with them, and the intrusiveness of the images were all significantly reduced at the post-MBCT assessment. Participants’ adapted SMQ scores were significantly increased following the MBCT intervention, suggesting that participants’ relationship with their intrusive images had changed in that they had developed a more ‘mindful’ and compassionate response to the images when they did occur. Effect sizes from pre- to post-intervention were medium to large (Cohen’s d = 0.75 - 1.50). All treatment gains were maintained at 3 month follow-up. Results suggest that MBCT may be an effective intervention for addressing intrusive imagery in hypochondriasis