Observational constraints on the modeling of SN1006

Experimental spectra and images of the supernova remnant SN1006 have been reported for radio, X-ray and TeV gamma-ray bands. Several comparisons between models and observations have been discussed in the literature, showing that the broad-band spectrum from the whole remnant as well as a sharpest radial profile of the X-ray brightness can be both fitted by adopting a model of SN1006 which strongly depends on the non-linear effects of the accelerated cosmic rays; these models predict post-shock magnetic field (MF) strengths of the order of 150 micro G. Here we present a new way to compare models and observations, in order to put constraints on the physical parameters and mechanisms governing the remnant. In particular, we show that a simple model based on the classic MHD and cosmic rays acceleration theories allows us to investigate the spatially distributed characteristics of SN1006 and to put observational constraints on the kinetics and MF. Our method includes modelling and comparison of the azimuthal and radial profiles of the surface brightness in radio, hard X-rays and TeV gamma-rays as well as the azimuthal variations of the electron maximum energy. In addition, this simple model also provides good fits to the radio-to-gamma-ray spectrum of SN1006. We find that our best-fit model predicts an effective MF strength inside SN1006 of 32 micro G, in good agreement with the `leptonic' model suggested by the HESS Collaboration (2010). Finally, some difficulties in both the `classic' and the non-linear models are discussed. A number of evidences about non-uniformity of MF around SN1006 are noted.Comment: 15 pages, 13 figures, accepted for publication on MNRA

The Dynamics of Stellar Coronae Harboring Hot-jupiters II. A Space Weather Event on A Hot-jupiter

We carry out a numerical simulation depicting the effects of a Coronal Mass Ejection (CME) event on a close-in giant planet in an extrasolar system. We drive the CME in a similar manner as in simulations of space weather events on Earth. The simulation includes the planetary orbital motion, which leads to the forming of a comet-like planetary magnetotail which is oriented almost perpendicular to the direction of propagation of the CME. The combination of this feature and the fact that the CME does not expand much by the time it reaches the planet leads to a unique CME-magnetosphere interaction, where the CME itself is highly affected by the presence of the planetary magnetosphere. We find that the planet is well-shielded from CME penetration, even for a relatively weak internal magnetic field. The planetary angular momentum loss associated with such an event is negligible compared to the total planetary angular momentum. We also find that the energy which is deposited in the magnetosphere is much higher than in the case of the Earth, and our simulation suggests there is a large-scale change in the orientation of the magnetosphere-ionosphere current system during the CME event.Comment: 16 pages, 10 figures, accepted to Ap

Rectangular core-collapse supernova remnants: application to Puppis A

Core-collapse supernova remnants are the gaseous nebulae of galactic interstellar media (ISM) formed after the explosive death of massive stars. Their morphology and emission properties depend both on the surrounding circumstellar structure shaped by the stellar wind-ISM interaction of the progenitor star and on the local conditions of the ambient medium. In the warm phase of the Galactic plane (n = 1/cm3, T = 8000 K), an organised magnetic field of strength 7 microG has profound consequences on the morphology of the wind bubble of massive stars at rest. In this paper we show through 2.5D magneto-hydrodynamical simulations, in the context of a Wolf-Rayet-evolving 35 Mo star, that it affects the development of its supernova remnant. When the supernova remnant reaches its middle age (15 to 20 kyr), it adopts a tubular shape that results from the interaction between the isotropic supernova ejecta and the anisotropic, magnetised, shocked stellar progenitor bubble into which the supernova blast wave expands. Our calculations for non-thermal emission, i.e. radio synchrotron and inverse Compton radiation, reveal that such supernova remnants can, due to projection effects, appear as rectangular objects in certain cases. This mechanism for shaping a supernova remnant is similar to the bipolar and elliptical planetary nebula production by wind-wind interaction in the low-mass regime of stellar evolution. If such a rectangular core-collapse supernova remnant is created, the progenitor star must not have been a runaway star. We propose that such a mechanism is at work in the shaping of the asymmetric core-collapse supernova remnant Puppis A.Comment: Accepted at MNRA

Straw blood cell count, growth, inhibition and comparison to apoptotic bodies

<p>Abstract</p> <p>Background</p> <p>Mammalian cells transform into individual tubular straw cells naturally in tissues and in response to desiccation related stress <it>in vitro</it>. The transformation event is characterized by a dramatic cellular deformation process which includes: condensation of certain cellular materials into a much smaller tubular structure, synthesis of a tubular wall and growth of filamentous extensions. This study continues the characterization of straw cells in blood, as well as the mechanisms of tubular transformation in response to stress; with specific emphasis placed on investigating whether tubular transformation shares the same signaling pathway as apoptosis.</p> <p>Results</p> <p>There are approximately 100 billion, unconventional, tubular straw cells in human blood at any given time. The straw blood cell count (SBC) is 45 million/ml, which accounts for 6.9% of the bloods dry weight. Straw cells originating from the lungs, liver and lymphocytes have varying nodules, hairiness and dimensions. Lipid profiling reveals severe disruption of the plasma membrane in CACO cells during transformation. The growth rates for the elongation of filaments and enlargement of rabbit straw cells is 0.6~1.1 (μm/hr) and 3.8 (μm³/hr), respectively. Studies using apoptosis inhibitors and a tubular transformation inhibitor in CACO2 cells and in mice suggested apoptosis produced apoptotic bodies are mediated differently than tubular transformation produced straw cells. A single dose of 0.01 mg/kg/day of p38 MAPK inhibitor in wild type mice results in a 30% reduction in the SBC. In 9 domestic animals SBC appears to correlate inversely with an animal's average lifespan (R²= 0.7).</p> <p>Conclusion</p> <p>Straw cells are observed residing in the mammalian blood with large quantities. Production of SBC appears to be constant for a given animal and may involve a stress-inducible protein kinase (P38 MAPK). Tubular transformation is a programmed cell survival process that diverges from apoptosis. SBCs may be an important indicator of intrinsic aging-related stress.</p

Ragweed as an Example of Worldwide Allergen Expansion

<p/> <p>Multiple factors are contributing to the expansion of ragweed on a worldwide scale. This review seeks to examine factors that may contribute to allergen expansion with reference to ragweed as a well-studied example. It is our hope that increased surveillance for new pollens in areas not previously affected and awareness of the influence the changing environment plays in allergic disease will lead to better outcomes in susceptible patients.</p

Atomic-Resolution Simulations Predict a Transition State for Vesicle Fusion Defined by Contact of a Few Lipid Tails

Membrane fusion is essential to both cellular vesicle trafficking and infection by enveloped viruses. While the fusion protein assemblies that catalyze fusion are readily identifiable, the specific activities of the proteins involved and nature of the membrane changes they induce remain unknown. Here, we use many atomic-resolution simulations of vesicle fusion to examine the molecular mechanisms for fusion in detail. We employ committor analysis for these million-atom vesicle fusion simulations to identify a transition state for fusion stalk formation. In our simulations, this transition state occurs when the bulk properties of each lipid bilayer remain in a lamellar state but a few hydrophobic tails bulge into the hydrophilic interface layer and make contact to nucleate a stalk. Additional simulations of influenza fusion peptides in lipid bilayers show that the peptides promote similar local protrusion of lipid tails. Comparing these two sets of simulations, we obtain a common set of structural changes between the transition state for stalk formation and the local environment of peptides known to catalyze fusion. Our results thus suggest that the specific molecular properties of individual lipids are highly important to vesicle fusion and yield an explicit structural model that could help explain the mechanism of catalysis by fusion proteins

A New Fluorescence-Based Method Identifies Protein Phosphatases Regulating Lipid Droplet Metabolism

In virtually every cell, neutral lipids are stored in cytoplasmic structures called lipid droplets (LDs) and also referred to as lipid bodies or lipid particles. We developed a rapid high-throughput assay based on the recovery of quenched BODIPY-fluorescence that allows to quantify lipid droplets. The method was validated by monitoring lipid droplet turnover during growth of a yeast culture and by screening a group of strains deleted in genes known to be involved in lipid metabolism. In both tests, the fluorimetric assay showed high sensitivity and good agreement with previously reported data using microscopy. We used this method for high-throughput identification of protein phosphatases involved in lipid droplet metabolism. From 65 yeast knockout strains encoding protein phosphatases and its regulatory subunits, 13 strains revealed to have abnormal levels of lipid droplets, 10 of them having high lipid droplet content. Strains deleted for type I protein phosphatases and related regulators (ppz2, gac1, bni4), type 2A phosphatase and its related regulator (pph21 and sap185), type 2C protein phosphatases (ptc1, ptc4, ptc7) and dual phosphatases (pps1, msg5) were catalogued as high-lipid droplet content strains. Only reg1, a targeting subunit of the type 1 phosphatase Glc7p, and members of the nutrient-sensitive TOR pathway (sit4 and the regulatory subunit sap190) were catalogued as low-lipid droplet content strains, which were studied further. We show that Snf1, the homologue of the mammalian AMP-activated kinase, is constitutively phosphorylated (hyperactive) in sit4 and sap190 strains leading to a reduction of acetyl-CoA carboxylase activity. In conclusion, our fast and highly sensitive method permitted us to catalogue protein phosphatases involved in the regulation of LD metabolism and present evidence indicating that the TOR pathway and the SNF1/AMPK pathway are connected through the Sit4p-Sap190p pair in the control of lipid droplet biogenesis

Metabolism and Regulation of Glycerolipids in the Yeast Saccharomyces cerevisiae

Due to its genetic tractability and increasing wealth of accessible data, the yeast Saccharomyces cerevisiae is a model system of choice for the study of the genetics, biochemistry, and cell biology of eukaryotic lipid metabolism. Glycerolipids (e.g., phospholipids and triacylglycerol) and their precursors are synthesized and metabolized by enzymes associated with the cytosol and membranous organelles, including endoplasmic reticulum, mitochondria, and lipid droplets. Genetic and biochemical analyses have revealed that glycerolipids play important roles in cell signaling, membrane trafficking, and anchoring of membrane proteins in addition to membrane structure. The expression of glycerolipid enzymes is controlled by a variety of conditions including growth stage and nutrient availability. Much of this regulation occurs at the transcriptional level and involves the Ino2–Ino4 activation complex and the Opi1 repressor, which interacts with Ino2 to attenuate transcriptional activation of UASINO-containing glycerolipid biosynthetic genes. Cellular levels of phosphatidic acid, precursor to all membrane phospholipids and the storage lipid triacylglycerol, regulates transcription of UASINO-containing genes by tethering Opi1 to the nuclear/endoplasmic reticulum membrane and controlling its translocation into the nucleus, a mechanism largely controlled by inositol availability. The transcriptional activator Zap1 controls the expression of some phospholipid synthesis genes in response to zinc availability. Regulatory mechanisms also include control of catalytic activity of glycerolipid enzymes by water-soluble precursors, products and lipids, and covalent modification of phosphorylation, while in vivo function of some enzymes is governed by their subcellular location. Genome-wide genetic analysis indicates coordinate regulation between glycerolipid metabolism and a broad spectrum of metabolic pathways