Nutritional value OF Bottle Gourd (Lagenaria siceraria) Seeds

Whole seeds, dehulled seeds and seed coats of bottle gourd seed (Lagenaria siceraria) were analysed for their proximate, amino acids and mineral compositions. The results of the analysis showed that, whole seed has highest content of moisture (17.5  0.21%) and ash (5.80  0.83%) while dehulled had highest amount of crude protein (35.0  0.48%) and crude lipid (39.22  1.48%) and seed coat contain highest amount of crude fiber (59.05  0.98%). The study showed a profile of seventeen amino acids (isoleucine, leucine, lysine, methionine, cysteine, phenylalanine, tyrosine, threonine, valine, alanine, arginine, aspartic acid, glutamic acid, glycine, histidine, proline and serine) with glutamic acid, leucine and aspartic acid being the predominant amino acid. The percentages (%) of essential and non-essential amino acids in dehulled seeds, whole seeds and seed coats were 44 vs 56, 41 vs 59 and 51 vs 49 respectively. The dehulled seeds contained essential amino acids that were found to be higher than WHO/FAO/UNU requirement. In whole seeds, threonine, lysine and lysine were found to be the most limiting amino acids. Seed coats were deficient in all the essential amino acids except for valine. Generally, the mineral composition of the seed was found to be relatively high, indicating the seed to be a good source of dietary elements, except for Ca, Zn, Co and Cr where very low values were obtained. Finally, the results of the study indicate that, bottle gourd seed is a potential source of protein, lipid, micro and macronutrients, and if properly utilised, could contribute in solving the problem of malnutrition and also serve as raw material for agro-based industries Keywords: Bottle gourd seeds, seed coat, proximate analysis, minerals and Amino acid composition.Global Journal of Pure and Applied Sciences Vol. 14 (3) 2008: pp. 301-30

Comparative Study of Antioxidant Activities of the Leaves and Stem of Ipomoea aquatica Forsk (Water Spinach)

Leaves and stem extracts of Ipomoea aquatic Forsk were analysed for their antioxidant activities. Folin-Ciocalteu and AlCl3 methods were used to quantify total phenolic and flavonoid contents while 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical assay was used to examine the antioxidant activity of the extracts. The respective total phenolic and flavonoid contents of the stem (18.00 ±  1.20 and 20.00 ± 1.40 μg Catechin equivalent, CEQ g-1 DW) show no significant (p > 0.05) difference when compared to leaves (16.00 ± 1.10 and 30.00 ± 2.1 μg CEQ g-1 DW). In DPPH scavenging assay, the reference standard i.e. vitamin C (IC50 = 0.0660μg/ml) had significantly (p<0.05) higher scavenging activity than the stem (IC50 = 35.96μg/ml) which in turn is significantly higher (p<0.05) than the leaves (IC50 = 176.92μg/ml). From the results it can be concluded that stem and leaves of Ipomoea aquatica had similar antioxidants activity.Keywords: Ipomoea aquatica, antioxidant, DPPH Assay, Scavenging activity, phenolic, flavonoi

Evaluation of Nutrient and Anti-nutrient Contents of Parkia biglobosa (L.) Flower

Nutritional and antinutritional contents of Parkia biglobosa flower were analysed using standard analytical methods. On dry weight basis, the flower had the following proximate compositions; ash (6.50 ± 1.00%), crude lipid (4.66 ± 0.29%), crude protein (6.77 ± 0.15%), available carbohydrate (78.9 ± 1.18%) and crude fibre (3.17 ± 0.29%). The calorific value was 384.7 kcal/100g. Mineral analysis indicates that the flower contain some essential minerals such as K, Na, Ca, Mg, and Zn, but was low in Cu, Mn, and Fe. The flower has low concentration of anti-nutritive factors: phytate (1.41±0.24mg %); oxalate (0.03±0.01mg %); hydrocyanic acid (0.17±0.01mg %) and nitrate (1.32±0.10mg %). The values are below the reference toxic standard levels. Therefore, P. biglobosa flower could supplement the microelements requirement, energy and to some extent protein

Determination of Nutritive Values of Garden Cress (Lepidium sativum L.) Leaves

The focus of this study was to investigate the nutritional values of Lepidium sativum leaves, a vegetable commonly consumed by the people of North-Western Nigeria. Sample of L. sativum leaves were collected from different farm lands at More town, Kware Local Government Area of Sokoto State. The leaves were dried then subjected to proximate, amino acids, minerals and antinutritional analyses. The results of proximate analysis indicate that the leaves had high crude protein (18.25%), crude fibre (9.31%) and ash (15.38%). The minera ls assay showed that the leaves are particularly high in potassium (1850.00 mg/100g), calcium (829.13 mg/100g), magnesium (160.60 mg/100g), sodium (141.13 mg/100g) and iron (63.47 mg/100g) with low level of phosphorus (4.10 mg/100g), manganese (5.74 mg/100g), copper (0.39 mg/100g) and chromium (0.36 mg/100g). The amino acid profile revealed that the leaf protein is generally low in lysine, sulphur containing amino acids (methionine and cystein), and threonine. Lysine was the most limiting amino acid in the leaves. In terms of antinutritional factors, the leaves had low concentrations of phytate (10.95 mg/100g), nitrate (0.05 mg/100g) and HCN (31.54mg/100g) with moderate amount of oxalate (337.50 mg/100g). Based on these nutrient contents, the leafy vegetable will have potential benefits as part of feeding programmes, as well as their promotion as part of composite die.Key words: Lepidium sativum, proximate analysis, amino acid, minerals, and antinutrients

Subcuteneousand Deep Lipomas in Exotic and Nigerian Indigenous Chickens: A Case Report

Three case reports describing the clinicopathological diagnosis and surgical management of subcutaneous lipomas in adult exotic and Nigerian indigenous chicken, as well as deep lipoma in exotic chickens are here presented. Two dead and one live chickens were presented to the Poultry Clinic, Veterinary Teaching Hospital, Ahmadu Bello University, Zaria, Nigeria, on the 15th October, 2016 and 13th August, 2017, respectively. The live indigenous hen was presented with a slow growing 8 month-old large mass on the right, ventro-lateral aspect of the neck. The mass was clinically observed to be pendulous, circumscribed, soft, lobulated, painless, subcutaneous seated and about 8 cm in diameter. The two dead exotic birds were earlier presented with the first having a similar lesion of different dimensions located in the ventro-lateral aspect of the left thigh and the second with lesions in the liver. Cytological evaluation of fine needle aspirates of the masses revealed well differentiated adipocytes interspersed with nucleated red blood cells. Consequently, the masses were tentatively diagnosed as subcutaneous and deep lipomas, and the management decision taken in the live chicken was surgical following standard procedures. Sections of the excised masses were fixed in 10% neutral buffered formalin and processed for histopathological examination. Histopathology revealed well differentiated adipocytes of uniform sizes interspersed with few blood vessels and connective tissue. Final diagnoses of lipomas were made. Depending on location and possible complications, subcutaneous lipomas may not be life threatening but they can be a source of discomfort to the patient and may cause emotional distress to their owners if not removed surgically. Deep lipomas may compromise organ functions leading to death

Having a lot of a good thing: multiple important group memberships as a source of self-esteem.

Copyright: © 2015 Jetten et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedMembership in important social groups can promote a positive identity. We propose and test an identity resource model in which personal self-esteem is boosted by membership in additional important social groups. Belonging to multiple important group memberships predicts personal self-esteem in children (Study 1a), older adults (Study 1b), and former residents of a homeless shelter (Study 1c). Study 2 shows that the effects of multiple important group memberships on personal self-esteem are not reducible to number of interpersonal ties. Studies 3a and 3b provide longitudinal evidence that multiple important group memberships predict personal self-esteem over time. Studies 4 and 5 show that collective self-esteem mediates this effect, suggesting that membership in multiple important groups boosts personal self-esteem because people take pride in, and derive meaning from, important group memberships. Discussion focuses on when and why important group memberships act as a social resource that fuels personal self-esteem.This study was supported by 1. Australian Research Council Future Fellowship (FT110100238) awarded to Jolanda Jetten (see http://www.arc.gov.au) 2. Australian Research Council Linkage Grant (LP110200437) to Jolanda Jetten and Genevieve Dingle (see http://www.arc.gov.au) 3. support from the Canadian Institute for Advanced Research Social Interactions, Identity and Well-Being Program to Nyla Branscombe, S. Alexander Haslam, and Catherine Haslam (see http://www.cifar.ca)

Measurement of the Z/gamma* + b-jet cross section in pp collisions at 7 TeV

The production of b jets in association with a Z/gamma* boson is studied using proton-proton collisions delivered by the LHC at a centre-of-mass energy of 7 TeV and recorded by the CMS detector. The inclusive cross section for Z/gamma* + b-jet production is measured in a sample corresponding to an integrated luminosity of 2.2 inverse femtobarns. The Z/gamma* + b-jet cross section with Z/gamma* to ll (where ll = ee or mu mu) for events with the invariant mass 60 < M(ll) < 120 GeV, at least one b jet at the hadron level with pT > 25 GeV and abs(eta) < 2.1, and a separation between the leptons and the jets of Delta R > 0.5 is found to be 5.84 +/- 0.08 (stat.) +/- 0.72 (syst.) +(0.25)/-(0.55) (theory) pb. The kinematic properties of the events are also studied and found to be in agreement with the predictions made by the MadGraph event generator with the parton shower and the hadronisation performed by PYTHIA.Comment: Submitted to the Journal of High Energy Physic

Decellularised skeletal muscles allow functional muscle regeneration by promoting host cell migration

Pathological conditions affecting skeletal muscle function may lead to irreversible volumetric muscle loss (VML). Therapeutic approaches involving acellular matrices represent an emerging and promising strategy to promote regeneration of skeletal muscle following injury. Here we investigated the ability of three different decellularised skeletal muscle scaffolds to support muscle regeneration in a xenogeneic immune-competent model of VML, in which the EDL muscle was surgically resected. All implanted acellular matrices, used to replace the resected muscles, were able to generate functional artificial muscles by promoting host myogenic cell migration and differentiation, as well as nervous fibres, vascular networks, and satellite cell (SC) homing. However, acellular tissue mainly composed of extracellular matrix (ECM) allowed better myofibre three-dimensional (3D) organization and the restoration of SC pool, when compared to scaffolds which also preserved muscular cytoskeletal structures. Finally, we showed that fibroblasts are indispensable to promote efficient migration and myogenesis by muscle stem cells across the scaffolds in vitro. This data strongly support the use of xenogeneic acellular muscles as device to treat VML conditions in absence of donor cell implementation, as well as in vitro model for studying cell interplay during myogenesis

Phenotypic Expression of ADAMTS13 in Glomerular Endothelial Cells

Background: ADAMTS13 is the physiological von Willebrand factor (VWF)-cleaving protease. The aim of this study was to examine ADAMTS13 expression in kidneys from ADAMTS13 wild-type (Adamts13+/+) and deficient (Adamts13-/-) mice and to investigate the expression pattern and bioactivity in human glomerular endothelial cells. Methodology/Principal Findings: Immunohistochemistry was performed on kidney sections from ADAMTS13 wild-type and ADAMTS13-deficient mice. Phenotypic differences were examined by ultramorphology. ADAMTS13 expression in human glomerular endothelial cells and dermal microvascular endothelial cells was investigated by real-time PCR, flow cytometry, immunofluorescence and immunoblotting. VWF cleavage was demonstrated by multimer structure analysis and immunoblotting. ADAMTS13 was demonstrated in glomerular endothelial cells in Adamts13+/+ mice but no staining was visible in tissue from Adamts13-/- mice. Thickening of glomerular capillaries with platelet deposition on the vessel wall was detected in Adamts13-/- mice. ADAMTS13 mRNA and protein were detected in both human endothelial cells and the protease was secreted. ADAMTS13 activity was demonstrated in glomerular endothelial cells as cleavage of VWF. Conclusions/Significance: Glomerular endothelial cells express and secrete ADAMTS13. The proteolytic activity could have a protective effect preventing deposition of platelets along capillary lumina under the conditions of high shear stress present in glomerular capillaries. © 2011 Tati et al.published_or_final_versio

Timing is everything: the regulation of type III secretion

Type Three Secretion Systems (T3SSs) are essential virulence determinants of many Gram-negative bacteria. The T3SS is an injection device that can transfer bacterial virulence proteins directly into host cells. The apparatus is made up of a basal body that spans both bacterial membranes and an extracellular needle that possesses a channel that is thought to act as a conduit for protein secretion. Contact with a host-cell membrane triggers the insertion of a pore into the target membrane, and effectors are translocated through this pore into the host cell. To assemble a functional T3SS, specific substrates must be targeted to the apparatus in the correct order. Recently, there have been many developments in our structural and functional understanding of the proteins involved in the regulation of secretion. Here we review the current understanding of protein components of the system thought to be involved in switching between different stages of secretion