325 research outputs found
Evolutionary responses by native species to major anthropogenic changes to their ecosystems: Pacific salmon in the Columbia River hydropower system
The human footprint is now large in all the Earthâs ecosystems, and construction of large dams in major river basins is among the anthropogenic changes that have had the most profound ecological consequences, particularly for migratory fishes. In the Columbia River basin of the western USA, considerable effort has been directed toward evaluating demographic effects of dams, yet little attention has been paid to evolutionary responses of migratory salmon to altered selective regimes. Here we make a first attempt to address this information gap. Transformation of the free-flowing Columbia River into a series of slackwater reservoirs has relaxed selection for adults capable of migrating long distances upstream against strong flows; conditions now favour fish capable of migrating through lakes and finding and navigating fish ladders. Juveniles must now be capable of surviving passage through multiple dams or collection and transportation around the dams. River flow patterns deliver some groups of juvenile salmon to the estuary later than is optimal for ocean survival, but countervailing selective pressures might constrain an evolutionary response toward earlier migration timing. Dams have increased the cost of migration, which reduces energy available for sexual selection and favours a nonmigratory life history. Reservoirs are a benign environment for many non-native species that are competitors with or predators on salmon, and evolutionary responses are likely (but undocumented). More research is needed to tease apart the relative importance of evolutionary vs. plastic responses of salmon to these environmental changes; this research is logistically challenging for species with life histories like Pacific salmon, but results should substantially improve our understanding of key processes. If the Columbia River is ever returned to a quasinatural, free-flowing state, remaining populations might face a Darwinian debt (and temporarily reduced fitness) as they struggle to re-evolve historical adaptations
LoCuSS: First Results from Strong-lensing Analysis of 20 Massive Galaxy Clusters at z~0.2
We present a statistical analysis of a sample of 20 strong lensing clusters
drawn from the Local Cluster Substructure Survey (LoCuSS), based on high
resolution Hubble Space Telescope imaging of the cluster cores and follow-up
spectroscopic observations using the Keck-I telescope. We use detailed
parameterized models of the mass distribution in the cluster cores, to measure
the total cluster mass and fraction of that mass associated with substructures
within R<250kpc.These measurements are compared with the distribution of
baryons in the cores, as traced by the old stellar populations and the X-ray
emitting intracluster medium. Our main results include: (i) the distribution of
Einstein radii is log-normal, with a peak and 1sigma width of
=1.16+/-0.28; (ii) we detect an X-ray/lensing mass discrepancy of
=1.3 at 3 sigma significance -- clusters with larger substructure
fractions displaying greater mass discrepancies, and thus greater departures
from hydrostatic equilibrium; (iii) cluster substructure fraction is also
correlated with the slope of the gas density profile on small scales, implying
a connection between cluster-cluster mergers and gas cooling. Overall our
results are consistent with the view that cluster-cluster mergers play a
prominent role in shaping the properties of cluster cores, in particular
causing departures from hydrostatic equilibrium, and possibly disturbing cool
cores. Our results do not support recent claims that large Einstein radius
clusters present a challenge to the CDM paradigm.Comment: 28 pages, 14 figures, accepted for publication in MNRAS, replaced
with accepted versio
Iron bioavailability: UK Food Standards Agency workshop report
The UK Food Standards Agency convened a group of expert scientists to review current research investigating factors affecting iron status and the bioavailability of dietary iron. Results presented at the workshop show menstrual blood loss to be the major determinant of body iron stores in premenopausal women. In the presence of abundant and varied food supplies, the health consequences of lower iron bioavailability are unclear and require further investigatio
Controls on luminescence signals in lake sediment cores:A study from Lake Suigetsu, Japan
The luminescence characteristics of sediments are driven by a range of environmental factors and can be used as indicators of both local and regional environmental shifts. Hence, rapid luminescence profiling techniques are increasingly employed during multiproxy analysis of sediment cores, overcoming the practical limitations of traditional (dating) methods. One emerging application of luminescence profiling is in the palaeoenvironmental investigation of lake cores. This study demonstrates the versatility of rapid core profiling using portable optically stimulated luminescence and laboratory profiling techniques for appraising the luminescence characteristics of the Lake Suigetsu (Japan) sediment cores. These techniques were employed across four key time periods, each selected for their unique environmental context and significance on either a local or global scale, in order to identify relationships between down-core luminescence and environmental change. We demonstrate that the luminescence characteristics of the cores are susceptible to a range of environmental perturbations and can therefore act as proxies of past change. Additionally, the quantification of these luminescence signals, alongside an assessment of dose rate variations down-core, supports the notion that future luminescence dating is feasible. The results of this analysis contribute to the wider understanding of the application of luminescence techniques â both profiling and dating â to lake sediment cores
Multiple novel non-canonically transcribed sub-genomic mRNAs produced by avian coronavirus infectious bronchitis virus
Funding: This work was supported by Biotechnology and Biological Sciences Research Council (BBSRC) grants BB/L003988/1 and 1645891, and strategic funding to The Pirbright Institute, BBS/E/I/00007035, BBS/E/I/00007034, BBS/E/I/00007037 and BBS/E/I/00007039.Coronavirus sub-genomic mRNA (sgmRNA) synthesis occurs via a process of discontinuous transcription involving complementary transcription regulatory sequences (TRSs), one (TRS-L) encompassing the leader sequence of the 5' untranslated region (UTR), and the other upstream of each structural and accessory gene (TRS-B). Several coronaviruses have an ORF located between the N gene and the 3'-UTR, an area previously thought to be non-coding in the Gammacoronavirus infectious bronchitis virus (IBV) due to a lack of a canonical TRS-B. Here, we identify a non-canonical TRS-B allowing for a novel sgmRNA relating to this ORF to be produced in several strains of IBV: Beaudette, CR88, H120, D1466, Italy-02 and QX. Interestingly, the potential protein produced by this ORF is prematurely truncated in the Beaudette strain. A single nucleotide deletion was made in the Beaudette strain allowing for the generation of a recombinant IBV (rIBV) that had the potential to express a full-length protein. Assessment of this rIBV in vitro demonstrated that restoration of the full-length potential protein had no effect on viral replication. Further assessment of the Beaudette-derived RNA identified a second non-canonically transcribed sgmRNA located within gene 2. Deep sequencing analysis of allantoic fluid from Beaudette-infected embryonated eggs confirmed the presence of both the newly identified non-canonically transcribed sgmRNAs and highlighted the potential for further yet unidentified sgmRNAs. This HiSeq data, alongside the confirmation of non-canonically transcribed sgmRNAs, indicates the potential of the coronavirus genome to encode a larger repertoire of genes than has currently been identified.Publisher PDFPeer reviewe
Identifying molecular mediators of the relationship between body mass index and endometrial cancer risk:a Mendelian randomization analysis
Endometrial cancer is the most common gynaecological cancer in high-income countries. Elevated body mass index (BMI) is an established modifiable risk factor for this condition and is estimated to confer a larger effect on endometrial cancer risk than any other cancer site. However, the molecular mechanisms underpinning this association remain unclear. We used Mendelian randomization (MR) to evaluate the causal role of 14 molecular risk factors (hormonal, metabolic and inflammatory markers) in endometrial cancer risk. We then evaluated and quantified the potential mediating role of these molecular traits in the relationship between BMI and endometrial cancer using multivariable MR.
Methods
Genetic instruments to proxy 14 molecular risk factors and BMI were constructed by identifying single-nucleotide polymorphisms (SNPs) reliably associated (P < 5.0 Ă 10â8) with each respective risk factor in previous genome-wide association studies (GWAS). Summary statistics for the association of these SNPs with overall and subtype-specific endometrial cancer risk (12,906 cases and 108,979 controls) were obtained from a GWAS meta-analysis of the Endometrial Cancer Association Consortium (ECAC), Epidemiology of Endometrial Cancer Consortium (E2C2) and UK Biobank. SNPs were combined into multi-allelic models and odds ratios (ORs) and 95% confidence intervals (95% CIs) were generated using inverse-variance weighted random-effects models. The mediating roles of the molecular risk factors in the relationship between BMI and endometrial cancer were then estimated using multivariable MR
Parameterization Effects in the analysis of AMI Sunyaev-Zel'dovich Observations
Most Sunyaev--Zel'dovich (SZ) and X-ray analyses of galaxy clusters try to
constrain the cluster total mass and/or gas mass using parameterised models and
assumptions of spherical symmetry and hydrostatic equilibrium. By numerically
exploring the probability distributions of the cluster parameters given the
simulated interferometric SZ data in the context of Bayesian methods, and
assuming a beta-model for the electron number density we investigate the
capability of this model and analysis to return the simulated cluster input
quantities via three rameterisations. In parameterisation I we assume that the
T is an input parameter. We find that parameterisation I can hardly constrain
the cluster parameters. We then investigate parameterisations II and III in
which fg(r200) replaces temperature as a main variable. In parameterisation II
we relate M_T(r200) and T assuming hydrostatic equilibrium. We find that
parameterisation II can constrain the cluster physical parameters but the
temperature estimate is biased low. In parameterisation III, the virial theorem
replaces the hydrostatic equilibrium assumption. We find that parameterisation
III results in unbiased estimates of the cluster properties. We generate a
second simulated cluster using a generalised NFW (GNFW) pressure profile and
analyse it with an entropy based model to take into account the temperature
gradient in our analysis and improve the cluster gas density distribution. This
model also constrains the cluster physical parameters and the results show a
radial decline in the gas temperature as expected. The mean cluster total mass
estimates are also within 1 sigma from the simulated cluster true values.
However, we find that for at least interferometric SZ analysis in practice at
the present time, there is no differences in the AMI visibilities between the
two models. This may of course change as the instruments improve.Comment: 19 pages, 13 tables, 24 figure
Detailed SZ study of 19 LoCuSS galaxy clusters: masses and temperatures out to the virial radius
We present 16-GHz AMI SZ observations of 19 clusters with L_X >7x10^37 W
(h50=1) selected from the LoCuS survey (0.142<z<0.295) and of A1758b, in the
FoV of A1758a. We detect 17 clusters with 5-23sigma peak surface brightnesses.
Cluster parameters are obtained using a Bayesian cluster analysis. We fit
isothermal beta-models to our data and assume the clusters are virialized (with
all the kinetic energy in gas internal energy). Our gas temperature, T_AMI, is
derived from AMI SZ data, not from X-ray spectroscopy. Cluster parameters
internal to r500 are derived assuming HSE. We find: (i) Different gNFW
parameterizations yield significantly different parameter degeneracies. (ii)
For h70 = 1, we find the virial radius r200 to be typically 1.6+/-0.1 Mpc and
the total mass M_T(r200) typically to be 2.0-2.5xM_T(r500).(iii) Where we have
found M_T X-ray (X) and weak-lensing (WL) values in the literature, there is
good agreement between WL and AMI estimates (with M_{T,AMI}/M_{T,WL}
=1.2^{+0.2}_{-0.3} and =1.0+/-0.1 for r500 and r200, respectively). In
comparison, most Suzaku/Chandra estimates are higher than for AMI (with
M_{T,X}/M_{T,AMI}=1.7+/-0.2 within r500), particularly for the stronger
mergers.(iv) Comparison of T_AMI to T_X sheds light on high X-ray masses: even
at large r, T_X can substantially exceed T_AMI in mergers. The use of these
higher T_X values will give higher X-ray masses. We stress that large-r T_SZ
and T_X data are scarce and must be increased. (v) Despite the paucity of data,
there is an indication of a relation between merger activity and SZ
ellipticity. (vi) At small radius (but away from any cooling flow) the SZ
signal (and T_AMI) is less sensitive to ICM disturbance than the X-ray signal
(and T_X) and, even at high r, mergers affect n^2-weighted X-ray data more than
n-weighted SZ, implying significant shocking or clumping or both occur even in
the outer parts of mergers.Comment: 45 pages, 33 figures, 13 tables Accepted for publication in MNRA
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