Experimental and Modelling of CO2 Absorption in a Bubble Column Using a Water-Based Nanofluid Containing Co-Doped SiO2 Nanoparticles

Acknowledgments The authors are grateful to Shiraz University for supporting this research.Peer reviewe

Comparative Study of Modified Chlorhexidine Toxicity, Chlorhexidine and Sodium Hypochlorite on Gingival Fibroblast Cells L929 In Vitro

One obvious and good properties of sodium hypochlorite is capacity of tissue solubility which has effective role in direct root canal cleaning, necrotic tissues and root canal system complexity. Chlorhexidine, in spite of all advantages that have than sodium hypochlorite, lacks this property. Superficial active factors or surfactants are materials that are used extensively as irritant, emulsifier, disinfection and solution additive. Surfactants are different types including benzalkonium chloride 4% and sodium lauryl Sulfate 2%. In this research, sodium dodecyl sulfate was used. Gingival fibroblast cells are kept in 25 cm2 flasks in 370C temperature. And then they are cultivated in Gibco Dulbecco’s Modified Eagle Medium (DMEM). 96 wells plates with 50 microliter are filled with growth medium with 2000 fibroblast and it is placed in incubator for 24 hours. After 24 hours, growth medium is thrown away and all plates are washed with phosphate buffered saline (PBS). All experimental steps are performed for preventing samples being contaminated under hood. The experiment was carried out as triplicate (3 wells cell). Results: In order to perform a research, Tukey-test and Duncan test were applied for explaining research variable. Results: toxicity rate of under tested solutions on fibroblast cells for group 1-4 are respectively (right to left) 13.75, 51.75, 20.75 and 51.75. Results of comparing means in four groups in landa level 4 indicated that groups are not significantly different in toxicity rate. results of this research indicated that this mixture can be used as an appropriate candidate for replacing sodium hypochlorite based on its disadvantages which needs to be studied more

The role of GlcNAc-PI-de-N-acetylase gene by gene knockout through homologous recombination and its consequences on survival, growth and infectivity of Leishmania major in in vitro and in vivo conditions

At present, there are no efficacious vaccines or effective drugs against leishmaniasis; therefore new and innovative control methods are urgently required. One way to achieve this important goal is through using reverse genetic engineering to evaluate important enzymes, proteins and macromolecules. One of the most important enzymes for Glycosylphosphatidylinositol (GPI) biosynthetic pathways is GlcNAc-PI-deN-acetylase (GPI12). The molecular constructs were cloned in Escherichia coli strain Top 10 and confirmed by molecular methods and were transfected by electroporation into Leishmania major. We demonstrated that two alleles of the GPI12 gene in L. major were successfully removed and enabling the generation of a null mutant, which supports the idea that GPI12 is not an essential gene for the growth and survival of Leishmania and the homozygous knockouts of Leishmania are able to survive. We were able to produce a mutant parasite that caused no damaged to the host. Further investigations are essential to check the safety profile in laboratory animal

The inhibitory effect of 6-gingerol and cisplatin on ovarian cancer and antitumor activity: In silico, in vitro, and in vivo

© 2023 Salari, Khosravi, Pourkhandani, Molaakbari, Salarkia, Keyhani, Sharifi, Tavakkoli, Sohbati, Dabiri, Ren and Shafie’ei. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). https://creativecommons.org/licenses/by/4.0/Background: Epithelial ovarian cancer is very common in women and causes hundreds of deaths per year worldwide. Chemotherapy drugs including cisplatin have adverse effects on patients’ health. Complementary treatments and the use of herbal medicines can help improve the performance of medicine. 6-Gingerol is the major pharmacologically active component of ginger. In this study, we compared the effects of 6-gingerol, cisplatin, and their combination in apoptotic and angiogenetic activities in silico, in test tubes, and in in vivo assays against two ovarian cancer cell lines: OVCAR-3 and human umbilical vein endothelial cells (HUVECs). Methods: The drug-treated cell lines were evaluated for their cytotoxicity, cell cycle, and apoptotic and angiogenetic gene expression changes. Results: The proportion of apoptosis treated by 6-gingerol coupled with cisplatin was significantly high. In the evaluation of the cell cycle, the combination therapy also showed a significant promotion of a higher extent of the S sequence. The expression of p53 level, Caspase-8, Bax, and Apaf1 genes was amplified again with combination therapy. Conversely, in both cell lines, the cumulative drug concentrations reduced the expression of VEGF, FLT1, KDR, and Bcl-2 genes. Similarly, in the control group, combination treatment significantly decreased the expression of VEGF, FLT1, KDR, and Bcl-2 genes in comparison to cisplatin alone. Conclusions: The findings of the present study demonstrated that the cisplatin and 6-gingerol combination is more effective in inducing apoptosis and suppressing the angiogenesis of ovarian cancer cells than using each drug alone.Peer reviewe

Biological properties and polyphenols content of Algerian Cistus salviifolius L. aerial parts

This study evaluated the in vitro antioxidant properties, antibacterial and antifungal activities and in vivo anti-inflammatory properties, and identifying the phenolic compounds in Cistus salviifolius. The methanolic leaf extract showed the highest antioxidant activity with 6.1±1.60 µg/ml IC50 value using DPPH· and 55.5±0.20 µg/ml using Reducing Power Activity. The study revealed that the butanolic leaf extract and the aqueous leaf infusion exhibited the strongest growth-inhibiting effect against all Gram positive and Gram negative strains tested, respectively, whereas the methanolic leaf extract showed the strongest antifungal activity against the yeast tested. The MIC value for the butanolic leaf extract was 4 mg/ml against Staphylococcus aureus, Bacillus subtilis and Escherichia coli. The pharmacotoxicological tests proved the safety of the aqueous leaf infusion, which exhibit a moderate anti-inflammatory effect, with a significant inhibition of the oedema development equal to 44.7% compared to 59.3% for the reference product diclofenac sodium. Methanolic extracts of the leaf and flower buds showed varied contents of polyphenols, flavonoids, and hydrolysable tannins; which were 228.4±11.4 mg GAE/g, 34.2±0.6 mg QE/g, and 36.9±2.6 mg TAE/g of the dry weight for leaves; and 241.1±5.4 mg GAE/g, 47.6±4.5 mg QE/g, and 22.0±1.3 mg TAE/g of the dry weight for flower buds, respectively. Analysis of the ethereal and butanolic leaf extracts using Reversed Phase High Performance Liquid Chromatographic Method coupled with a Photodiode-Array Detector identified thirteen phenolic compounds, including ascorbic acid, vanillic acid, gallic acid, quercetin, and orientin. DOI: http://dx.doi.org/10.5281/zenodo.656150

Condom Use and its Associated Factors Among Iranian Youth: Results From a Population-Based Study

Abstract Background: Given the young structure of Iran’s population and the fact that extramarital sexual relationships are both prohibited by legislation and shunned by society and religion, examining condom use practices among Iranian youth is highly important. The aim of this study was to explore condom use and its correlates among Iranian young adults. Methods: In a sample of 3,045 individuals aged 19-29 who were recruited from a nation-wide study, we analyzed data from 633 participants who reported a history of extramarital sex. Subjects were asked about their condom use practices during their last penetrative sex. Data were collected through a self-administered questionnaire where the respondents completed the survey on their own and passed it to trained gender-matched interviewers. Multivariable regression models were constructed to report adjusted odds ratios (AOR) along with 95% CI. Results: Of the 633 participants, 222 (35.1%) reported condom use at last sex. Men reported significantly higher condom use than women (38.5% vs. 25.7%). Having a stable job (AOR = 1.86, 95% CI: 1.01, 3.43), higher knowledge of condom use (AOR = 1.57, 95% CI: 1.03, 2.37) and sexual transmission of HIV (AOR = 1.83, 95% CI: 1.18, 2.85) were positively associated with condom use at last sex. Conversely, experience of sex under the influence of substances (AOR = 0.66, 95% CI: 0.45, 0.94) was significantly associated with reduced odds of condom use at last sex. Conclusion: This study shows that only one out of every three young adults reported using condoms at last sex. While educational programs are helpful, multi-sectoral approaches (eg, individual-, community-, and structural-level interventions) are required to change sexual behaviours towards safe sex practices and reinforce negotiating condom use among youth

Correlates of Alcohol Consumption and Drug Injection among Homeless Youth: A Case Study in the Southeast of Iran

Background: Alcohol use and drug injection are prevalent among homeless youths. The aim of this study wasto identify the associated factors of alcohol consumption and drug injection among homeless youths aged18-29 years.Methods: Data on 202 homeless youths (111 males and 91 females) were collected using a standardizedquestionnaire and face-to-face interview. Lasso logistic regression was applied to determine the impact ofassociated factors on alcohol consumption and drug injection.Findings: The mean age of the participants was 26.30 ± 3.19 years. Also, the prevalence of alcoholconsumption and drug injection was 33.0% [95% confidence interval (CI): 30-36] and 4.0% (95% CI: 0-8),respectively; 6 people (3.0%) consumed alcohol and injected drugs at the same time. Correlates of alcoholconsumption and drug injection were male sex [odds ratio (OR)Alc = 5.7], age (ORAlc = 0.96 and ORDI = 0.98),bachelor or higher education level (ORAlc = 1.34), non-Iranian nationality (ORAlc = 0.05 and ORDI = 0.18),food score (ORDI = 0.92), smoking (ORAlc = 2.05), substance use (ORAlc = 1.12), opposite sex relationship(ORAlc = 1.6), homosexual relationship (ORAlc = 3.56 and ORDI = 2.69), and mental disorder (ORAlc = 0.99).Conclusion: Based on our findings, it seems that the homeless youth are more desired to use alcohol and druginjection, whereas the prevalence of alcohol consumption and drug injection in homeless youth was higherthan general youth population in Iran. Therefore, some suitable solutions are needed to prevent thehomelessness. Also, the effective variables that were identified in this study for alcohol use and drug injectioncan help design and implement beneficial interventions

Global, regional, and national cancer incidence, mortality, years of life lost, years lived with disability, and disability-Adjusted life-years for 29 cancer groups, 1990 to 2017 : A systematic analysis for the global burden of disease study

Importance: Cancer and other noncommunicable diseases (NCDs) are now widely recognized as a threat to global development. The latest United Nations high-level meeting on NCDs reaffirmed this observation and also highlighted the slow progress in meeting the 2011 Political Declaration on the Prevention and Control of Noncommunicable Diseases and the third Sustainable Development Goal. Lack of situational analyses, priority setting, and budgeting have been identified as major obstacles in achieving these goals. All of these have in common that they require information on the local cancer epidemiology. The Global Burden of Disease (GBD) study is uniquely poised to provide these crucial data. Objective: To describe cancer burden for 29 cancer groups in 195 countries from 1990 through 2017 to provide data needed for cancer control planning. Evidence Review: We used the GBD study estimation methods to describe cancer incidence, mortality, years lived with disability, years of life lost, and disability-Adjusted life-years (DALYs). Results are presented at the national level as well as by Socio-demographic Index (SDI), a composite indicator of income, educational attainment, and total fertility rate. We also analyzed the influence of the epidemiological vs the demographic transition on cancer incidence. Findings: In 2017, there were 24.5 million incident cancer cases worldwide (16.8 million without nonmelanoma skin cancer [NMSC]) and 9.6 million cancer deaths. The majority of cancer DALYs came from years of life lost (97%), and only 3% came from years lived with disability. The odds of developing cancer were the lowest in the low SDI quintile (1 in 7) and the highest in the high SDI quintile (1 in 2) for both sexes. In 2017, the most common incident cancers in men were NMSC (4.3 million incident cases); tracheal, bronchus, and lung (TBL) cancer (1.5 million incident cases); and prostate cancer (1.3 million incident cases). The most common causes of cancer deaths and DALYs for men were TBL cancer (1.3 million deaths and 28.4 million DALYs), liver cancer (572000 deaths and 15.2 million DALYs), and stomach cancer (542000 deaths and 12.2 million DALYs). For women in 2017, the most common incident cancers were NMSC (3.3 million incident cases), breast cancer (1.9 million incident cases), and colorectal cancer (819000 incident cases). The leading causes of cancer deaths and DALYs for women were breast cancer (601000 deaths and 17.4 million DALYs), TBL cancer (596000 deaths and 12.6 million DALYs), and colorectal cancer (414000 deaths and 8.3 million DALYs). Conclusions and Relevance: The national epidemiological profiles of cancer burden in the GBD study show large heterogeneities, which are a reflection of different exposures to risk factors, economic settings, lifestyles, and access to care and screening. The GBD study can be used by policy makers and other stakeholders to develop and improve national and local cancer control in order to achieve the global targets and improve equity in cancer care. © 2019 American Medical Association. All rights reserved.Peer reviewe

National, sub-national, and risk-attributed burden of thyroid cancer in Iran from 1990 to 2019

An updated exploration of the burden of thyroid cancer across a country is always required for making correct decisions. The objective of this study is to present the thyroid cancer burden and attributed burden to the high Body Mass Index (BMI) in Iran at national and sub-national levels from 1990 to 2019. The data was obtained from the GBD 2019 study estimates. To explain the pattern of changes in incidence from 1990 to 2019, decomposition analysis was conducted. Besides, the attribution of high BMI in the thyroid cancer DALYs and deaths were obtained. The age-standardized incidence rate of thyroid cancer was 1.57 (95% UI: 1.33–1.86) in 1990 and increased 131% (53–191) until 2019. The age-standardized prevalence rate of thyroid cancer was 30.19 (18.75–34.55) in 2019 which increased 164% (77–246) from 11.44 (9.38–13.85) in 1990. In 2019, the death rate, and Disability-adjusted life years of thyroid cancer was 0.49 (0.36–0.53), and 13.16 (8.93–14.62), respectively. These numbers also increased since 1990. The DALYs and deaths attributable to high BMI was 1.91 (0.95–3.11) and 0.07 (0.04–0.11), respectively. The thyroid cancer burden and high BMI attributed burden has increased from 1990 to 2019 in Iran. This study and similar studies’ results can be used for accurate resource allocation for efficient management and all potential risks’ modification for thyroid cancer with a cost-conscious view

Incremental substitution of Ni with Mn in NiFe2O4 to largely enhance its supercapacitance properties

Abstract By using a facile hydrothermal method, we synthesized Ni1−xMnxFe2O4 nanoparticles as supercapacitor electrode materials and studied how the incremental substitution of Ni with Mn would affect their structural, electronic, and electrochemical properties. X-ray diffractometry confirmed the single-phase spinel structure of the nanoparticles. Raman spectroscopy showed the conversion of the inverse structure of NiFe2O4 to the almost normal structure of MnFe2O4. Field-emission scanning electron microscopy showed the spherical shape of the obtained nanoparticles with a size in the range of 20–30 nm. Optical bandgaps were found to decrease as the content of Mn increased. Electrochemical characterizations of the samples indicated the excellent performance and the desirable cycling stability of the prepared nanoparticles for supercapacitors. In particular, the specific capacitance of the prepared Ni1−xMnxFe2O4 nanoparticles was found to increase as the content of Mn increased, reaching the highest specific capacitance of 1,221 F/g for MnFe2O4 nanoparticles at the current density of 0.5 A/g with the corresponding power density of 473.96 W/kg and the energy density of 88.16 Wh/kg. We also demonstrated the real-world application of the prepared MnFe2O4 nanoparticles. We performed also a DFT study to verify the changes in the geometrical and electronic properties that could affect the electrochemical performance