Experiment-based Comparison of Prediction Methods for Pump Head Degradation with Viscous and Power-law Fluids

Although several methods are known to calculate pump performance with highly viscous and non-Newtonian fluids, research has not yet determined all the key parameters of these predictions. It is unclear how these parameters depend on the pump geometry and the delivered fluid rheology, which can vary widely in the chemical industry. In our study, the performance curves of a radial centrifugal pump with a viscous Newtonian glycerol solution and a non-Newtonian power-law fluid were experimentally compared. The head degradation of the pump was also presumed with the ANSI/HI and the Ofuchi methods, which are evident and commonly used for viscous Newtonian fluids, but not for non-Newtonians. The required constants were estimated based on experimental data for both models, and the Ofuchi method was adapted to power-law fluid. Based on our results, the Ofuchi method proved to apply for head degradation prediction with the examined power-law fluid. This work is licensed under a Creative Commons Attribution 4.0 International License

CD11c/CD18 Dominates Adhesion of Human Monocytes, Macrophages and Dendritic Cells over CD11b/CD18.

Complement receptors CR3 (CD11b/CD18) and CR4 (CD11c/CD18) belong to the family of beta2 integrins and are expressed mainly by myeloid cell types in humans. Previously, we proved that CR3 rather than CR4 plays a key role in phagocytosis. Here we analysed how CD11b and CD11c participate in cell adhesion to fibrinogen, a common ligand of CR3 and CR4, employing human monocytes, monocyte-derived macrophages (MDMs) and monocyte-derived dendritic cells (MDDCs) highly expressing CD11b as well as CD11c. We determined the exact numbers of CD11b and CD11c on these cell types by a bead-based technique, and found that the ratio of CD11b/CD11c is 1.2 for MDDCs, 1.7 for MDMs and 7.1 for monocytes, suggesting that the function of CD11c is preponderant in MDDCs and less pronounced in monocytes. Applying state-of-the-art biophysical techniques, we proved that cellular adherence to fibrinogen is dominated by CD11c. Furthermore, we found that blocking CD11b significantly enhances the attachment of MDDCs and MDMs to fibrinogen, demonstrating a competition between CD11b and CD11c for this ligand. On the basis of the cell surface receptor numbers and the measured adhesion strength we set up a model, which explains the different behavior of the three cell types