Guidelines for the monitoring of Rosalia alpina

Rosalia alpina (Linnaeus, 1758) is a large longhorn beetle (Coleoptera: Cerambycidae) which is protected by the Habitats Directive and which typically inhabits beech forests characterised by the presence of mature, dead (or moribund) and sun-exposed trees. A revision of the current knowledge on systematics, ecology and conservation of R. alpina is reported. The research was carried out as part of the LIFE MIPP project which aims to find a standard monitoring method for saproxylic beetles protected in Europe. For monitoring this species, different methods were tested and compared in two areas of the Apennines, utilising wild trees, logs and tripods (artificially built with beech woods), all potentially suitable for the reproduction of the species. Even if all methods succeeded in the survey of the target species, these results showed that the use of wild trees outperformed other methods. Indeed, the use of wild trees allowed more adults to be observed and required less intensive labour. However, monitoring the rosalia longicorn on wild trees has the main disadvantage that they can hardly be considered “standard sampling units”, as each tree may be differently attractive to adults. Our results demonstrated that the most important factors influencing the attraction of single trunks were wood volume, sun-exposure and decay stage. Based on the results obtained during the project LIFE MIPP, as well as on a literature review, a standard monitoring method for R. alpina was developed

A first assessment of genetic variability in the longhorn beetle Rosalia alpina (Coleoptera: Cerambycidae) from the Italian Apennines

The Rosalia longicorn (Rosalia alpina) is a strictly protected saproxylic beetle, widely distributed in Central and Southern Europe and mainly associated with ancient beech forests. To improve knowledge about the conservation status of R. alpina in Italy, available molecular markers (microsatellites and mitochondrial cytochrome c oxidase I(COI)) were tested for the first time on Italian populations. The study was performed in four sampling sites distributed in two areas placed in Northern (“Foreste Casentinesi” National Park) and Central Apennines (“Abruzzo, Lazio and Molise” National Park) where populational data about Rosalia longicorn were collected in the framework of the European LIFE MIPP Project. The genetic relationship among Apennine and Central/South-eastern European populations was explored by a comparison with mitochondrial DNA (mtDNA) data from literature. Microsatellite markers were only partially informative when applied to R. alpina Italian individuals, although providing some preliminary indication on an extensive gene flow among populations from the Apennines and local ongoing processes of genetic erosion. Genetic data are consistent with previous ecological data suggesting that the maintenance of variability in this species could be related to both habitat continuity and preservation of large senescent or standing dead trees in forests. Finally, a peculiar origin of the Apennine populations of R. alpina from a putative “Glacial Refugium” in Italy was inferred through COI data. The high genetic distance scored among the analysed populations and those from Central and South-eastern Europe indicates that the R. alpina deme from Apennine Mountains might represent a relevant conservation unit in Europe. Further genetic analyses will allow assessing other possible conservation units of R. alpina and, thus, defining large-scale conservation strategies to protect this endangered longhorn beetle in Europe

Assessing habitat-related disturbance in bird communities: Applying hemeroby and generalism as indicators

We tested the application of the concept of hemeroby and generalism at community level, on a set of birds occurring in various habitats of central Italy characterized by different level of disturbance. In each habitat-related bird community, we applied the recently published species-specific score in hemeroby (a proxy of habitat-related disturbance; HSi) and hemerobiotic diversity (a proxy of generalism; H’Hi) to local species frequency, obtaining weighted values at community level (HStot and H’Htot). The relationship between HStot vs. H’Htot showed an increasing trend moving from reed beds through forests and mosaics to urban communities. Quadratic model (best fit) evidenced a significant correlation between these variables and a tendency toward a hump-shaped curve, corroborating results already observed at species level (intermediate generalism hypothesis). The co-inertia analysis discriminated four groups of habitat-related communities, characterized by species with different levels of disturbance-sensitivity (expressed by HSi) and generalism (expressed by hemerobiotic diversity; H’Hi): (i) forest type-related, where mature wood communities were separated from a coppiced wood one; (ii) communities of moderately disturbed agricultural habitats; (iii) communities embedded in highly disturbed mosaics, and (iv) a group including either a highly disturbed urban habitat or a low disturbed wetland reed bed, with highly specialized species (respectively, synanthropic species and water-related species). Total scores in hemeroby and hemerobiotic diversity, expressing the composition in species with different disturbance preference and generalism, might act as good community-based indicators of degree of naturalness, especially for forest habitat types

An overview of the clinical use of filter paper in the diagnosis of tropical diseases.

Tropical infectious diseases diagnosis and surveillance are often hampered by difficulties of sample collection and transportation. Filter paper potentially provides a useful medium to help overcome such problems. We reviewed the literature on the use of filter paper, focusing on the evaluation of nucleic acid and serological assays for diagnosis of infectious diseases using dried blood spots (DBS) compared with recognized gold standards. We reviewed 296 eligible studies and included 101 studies evaluating DBS and 192 studies on other aspects of filter paper use. We also discuss the use of filter paper with other body fluids and for tropical veterinary medicine. In general, DBS perform with sensitivities and specificities similar or only slightly inferior to gold standard sample types. However, important problems were revealed with the uncritical use of DBS, inappropriate statistical analysis, and lack of standardized methodology. DBS have great potential to empower healthcare workers by making laboratory-based diagnostic tests more readily accessible, but additional and more rigorous research is needed

The CAESAR project for the ASI space weather infrastructure

This paper presents the project Comprehensive spAce wEather Studies for the ASPIS prototype Realization (CAESAR), which aims to tackle the relevant aspects of Space Weather (SWE) science and develop a prototype of the scientific data centre for Space Weather of the Italian Space Agency (ASI) called ASPIS (ASI SPace Weather InfraStructure). To this end, CAESAR involves the majority of the SWE Italian community, bringing together 10 Italian institutions as partners, and a total of 92 researchers. The CAESAR approach encompasses the whole chain of phenomena from the Sun to Earth up to planetary environments in a multidisciplinary, comprehensive, and unprecedented way. Detailed and integrated studies are being performed on a number of well-observed “target SWE events”, which exhibit noticeable SWE characteristics from several SWE perspectives. CAESAR investigations synergistically exploit a great variety of different products (datasets, codes, models), both long-standing and novel, that will be made available in the ASPIS prototype: this will consist of a relational database (DB), an interface, and a wiki-like documentation structure. The DB will be accessed through both a Web graphical interface and the ASPIS.py module, i.e., a library of functions in Python, which will be available for download and installation. The ASPIS prototype will unify multiple SWE resources through a flexible and adaptable architecture, and will integrate currently available international SWE assets to foster scientific studies and advance forecasting capabilities

Exploring cosmic origins with CORE: Mitigation of systematic effects

We present an analysis of the main systematic effects that could impact the measurement of CMB polarization with the proposed CORE space mission. We employ timeline-to-map simulations to verify that the CORE instrumental set-up and scanning strategy allow us to measure sky polarization to a level of accuracy adequate to the mission science goals. We also show how the CORE observations can be processed to mitigate the level of contamination by potentially worrying systematics, including intensity-to-polarization leakage due to bandpass mismatch, asymmetric main beams, pointing errors and correlated noise. We use analysis techniques that are well validated on data from current missions such as Planck to demonstrate how the residual contamination of the measurements by these effects can be brought to a level low enough not to hamper the scientific capability of the mission, nor significantly increase the overall error budget. We also present a prototype of the CORE photometric calibration pipeline, based on that used for Planck, and discuss its robustness to systematics, showing how CORE can achieve its calibration requirements. While a fine-grained assessment of the impact of systematics requires a level of knowledge of the system that can only be achieved in a future study phase, the analysis presented here strongly suggests that the main areas of concern for the CORE mission can be addressed using existing knowledge, techniques and algorithms

Overwintering ability and habitat preference of Morimus asper: a two-year mark-recapture study with implications for conservation and forest management

Morimus asper is a saproxylic longhorn beetle associated with mature forests. Its flightless condition makes its colonization of new trees in fragmented areas difficult. Our 2-year mark-recapture study revealed new information about the survival ability, population dynamics and habitat preference of M. asper in three beech forests of central Italy. Daily surveys were performed from May to July in 2013 and 2014, inspecting suitable trees for hosting the species. A low percentage of overwintered adults was observed for both sexes, with a very long lifespan: 282–409 days. Almost all individuals were recaptured on the tree of their first capture, and dispersal (20–451 m) was recorded only in thirteen individuals. Adult phenology showed two peaks of activity (middle of May and middle of June). Population estimates varied significantly according to site and year. Mean daily temperature affected recapture probability and total captures (optimal range of 15–26 °C). The species showed preference for huge lying trees, branches on the ground, a low percentage of debarked surface and high canopy closure. M. asper showed a high survival probability, but the few movements observed, combined with its flightless condition, suggest strongly that this species has a low dispersal ability, highly threatened by local fragmentation. Furthermore, almost all captures were made on the same tree, making this a “key tree” for population viability. Forest management techniques should retain many lying trees, abundant coarse woody debris and a dense canopy that ensures a ‘cooler’ temperature in the understory, favouring M. asper and its conservatio

Detection of Epstein Barr virus in formalin-fixed paraffin tissues by fluorescent direct in situ PCR

Specific viral laboratory diagnosis of primary Epstein-Barr Virus (EBV) infection is usually based on antibody-detection assays. However, molecular detection is also considered the reference standard assay for diagnosis of central nervous system infections and of most cases of nasopharyngeal carcinoma (NPC). One-step or nested polymerase chain reaction (PCR) has rapidly replaced immunological assays based on virus-specific Ig antibodies for the laboratory diagnosis of Herpesvirus infections, even if serological methods are considered an additional tool for defining clinical diagnosis. In this article, we will present a rapid, sensitive and robust molecular tool for the viral detection of EBV (EBNA-1) within tissue specimens by making use of in situ PCR (IS-PCR)