Tropheryma whipplei in Fecal Samples from Children, Senegal

We tested fecal samples from 150 healthy children 2–10 years of age who lived in rural Senegal and found the prevalence of Tropheryma whipplei was 44%. Unique genotypes were associated with this bacterium. Our findings suggest that T. whipplei is emerging as a highly prevalent pathogen in sub-Saharan Africa

La imaginación como herramienta de conocimiento en Ibn ‘Arabi: Un estudio sobre el acto de creación y adoración como acto de imaginación y representación consciente simultáneo a la trascendencia de dicha representación.

Treballs Finals de Grau d'Estudis Àrabs i Hebreus. Facultat de Filologia. Universitat de Barcelona, Curs: 2015-2016, Tutor: Miquel Forcada Nogues[spa] Ibn ‘Arabī, místico andalusí de mediados del siglo XII, es conocido por la universalidad de su doctrina en tanto que trasciende, en lo que se refiere a la explicación y el entendimiento, las ideas de Dios, hombre y mundo, haciéndolas accesibles a cualquier filósofo o religioso letrado de cualquier otra disciplina o religión. La actitud de su doctrina reside en el perfeccionamiento de uno mismo a través de la búsqueda de conocimiento, ya sea por medio de la reflexión o de la imaginación. Concretamente, el papel que la imaginación juega en su visión del mundo es el de la confluencia de opuestos, evitando cualquier extremismo y permitiendo todo lo existente manifestarse en diferentes realidades, sin que una perspectiva elimine a la otra. Esto supone una atrevida asunción de responsabilidad por parte del autor, pues la realidad de las cosas depende del punto de vista desde el cual sean observadas. Esta posición respecto al conocimiento es inteligente al ofrecer un elevado grado de diversidad, pues si no se desestima nada del todo, todo puede utilizarse como herramienta hacia el conocimiento.[eng] Ibn ‘Arabī, mystical andalusí from the second half of the 12th century, is known for its universal doctrine which trascends, in relation to explanation and understanding, the ideas of God, man and world, making them accessible to any philosopher or religious scholar of any other discipline or religious order. The attitude of his doctrine lies in self-oriented perfectionism through the pursuit of knowledge, either through reflection or imagination. Specifically, the role that imagination plays in his view of the world is the confluence of opposites, avoiding any extremism and allowing all the existing manifest in different realities, without one perspective denying the other. This implies a bold assumption of responsability by the hand of the author, as the reality of things depends on the point of view form which they are observed. This position regarding to knowledge is smart as it offers a high grade of diversity, as if you do not reject completely anything, everything may be used as a tool to knowledge

Tropheryma whipplei in Children with Gastroenteritis

This bacterium may be an etiologic agent of gastroenteritis

Doctor of Philosophy

dissertationThe main aim of this study was to examine the effectiveness of an innovative health prevention program, the Healthy and Fit Adults Program (HFAP). The HFAP consists of five sessions designed after an effective evidence-based family skills training intervention, the Strengthening Families Program, which uses Bandura's (1986) Social Cognitive Theory in order to increase protective factors against health risk behaviors. The HFAP intervention included two elements (reflecting on gratitude and expressive writing as the vehicle to reflect on gratitude) as means to improve healthy behaviors. It was hypothesized that those participating in the HFAP would increase health protective factors against obesity (physical activity) and decrease health risk factors for obesity (excessive weight and depression). This study employed a quasi-experimental design (no treatment comparison and treatment groups) in order to assess the impact of the HFAP on the health behaviors of 48 voluntary participants ages 31 to 45. Standardized measures included self-reported scales to measure physical activity (IPAQ), gratitude (GQ-6), and depression (CES-D) as well as objective measures (pedometer and BMI). Data were collected and analyzed using parametric and nonparametric tests (RM-ANOVAS, ANCOVA, Paired Samples t test, Wilcoxon Signed Ranks Tests, Spearman Correlation Coefficient). Results showed statistically significant increases in self-reported gratitude as well as a significant decrease in depression scores within the experimental group from pretest to posttest. Self-reported physical activity measured in METs also increased significantly within the experimental group from pretest to posttest. These results were only statistically significant when baseline differences between groups were not accounted for. When baseline differences were considered (ANCOVA), the differences in main outcomes (physical activity in METs and steps, gratitude, BMI, and depression) between comparison and treatment groups from pretest to posttest were not statistically significant. There was, however, a statistically significant moderate negative correlation between levels of gratitude and depression among participants. In this study, reflecting and practicing gratitude seemed to contribute to healthier psychological self-perceptions of participants (i.e., improved gratitude and decreased depression). Findings are discussed and suggestions for future research and interventions aiming to prevent obesity among adults are provided

Pràctica sobre el càlcul de les propietats fonamentals del so a partir de la freqüència d'ultrasò d'ecolocalització dels ratpenats

El ratpenat o quiròpter emet unes freqüències d’ultrasò en forma de crit (generalment entre 20 i 120 kHz). En aquesta pràctica treballarem conceptes relacionats amb les característiques d`aquest so, detectat amb el detector Bat Tune: freqüència f, període T, freqüència angular o pulsació w, longitud d´ona λ i el nombre d'ona k.The different species of bats emits some ultrasound frequencies in a form of ultrasound (usually between 20 and 120 kHz). In this practice we will work on concepts related to the characteristics of this sound, detected with the Bat Tune detector: frequency f, period T, angular frequency or pulsation w, length of wave λ and the number of wave k

False positive PCR detection of Tropheryma whipplei in the saliva of healthy people

<p>Abstract</p> <p>Background</p> <p><it>Tropheryma whipplei</it>, the agent of Whipple's disease (WD), has been recently isolated and the genomes of two isolates have been fully sequenced. Previous diagnosis tools for the diagnosis of the disease used sequence analysis of the 16S rRNA gene. Using this target gene, the high percentage of detection of the bacterium in saliva of healthy people was in contrast to the negative results obtained with specific target genes. The aim of our study was to compare previously published primers targeting the 16S rRNA gene to real-time PCR with Taqman* probes targeting specific repeat genes only found in the genome of <it>T. whipplei </it>in a series of 57 saliva from healthy people.</p> <p>Results</p> <p>Although the specific real-time PCR assays with both primers and probes were negative for all the samples, 13 out of 57 samples were positive with different primers previously reported targeting the 16S rRNA gene. Among the positive samples, 8 yielded a 231-bp sequence that was 99.1% identical to that of <it>Actinomyces odontolyticus</it>, 2 yielded a 226-bp that was 99.6% identical to that of <it>A. turicensis</it>, and 3 yielded a 160-bp sequence that was 98.5% identical to that of <it>Capnocytophaga gingivalis</it>. We found that the <it>C. gingivalis </it>and <it>A. odontolyticus </it>16S rRNA sequences obtained in our study share more than 80% homology with the corresponding 16S rRNA sequences of the <it>T. whipplei </it>genomes especially at 5' and 3' end.</p> <p>Conclusion</p> <p>Asymptomatic carriers of <it>T. whipplei </it>in saliva may exist but their prevalence is much lower than those previously reported. Testing the specificity of designed primers is critical to avoid false positive detection of <it>T. whipplei</it>. In atypical case we recommend to test two different specific target genes before concluding.</p

Tropheryma whipplei in Patients with Pneumonia

This bacterium may be an etiologic agent of pneumonia

“Ezakiella massiliensis” sp. nov., a new bacterial species isolated from human female genital tract

AbstractWe report the primary characteristics of “Ezakiella massiliensis” strain Marseille P2951 (= DSM 103122 = CSUR P2951), a new member of the Ezakiella genus. Strain Marseille P2951 was isolated from a vaginal sample taken from an asymptomatic 20-year-old woman who had sex with another woman who had bacterial vaginosis