Diving into the mouse macrophage cytoskeletal proteome upon the interaction with candida albicans

Comunicaciones a congreso

The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation

The base of the primary cilium contains a zone of condensed membranes whose importance is not known. Here, we have studied the involvement of MAL, a tetraspanning protein that exclusively partitions into condensed membrane fractions, in the condensation of membranes at the ciliary base and investigated the importance of these membranes in primary cilium formation. We show that MAL accumulates at the ciliary base of epithelial MDCK cells. Knockdown of MAL expression resulted in a drastic reduction in the condensation of membranes at the ciliary base, the percentage of ciliated cells and the length of the cilia, but did not affect the docking of the centrosome to the plasma membrane or produce missorting of proteins to the pericentriolar zone or to the membrane of the remaining cilia. Rab8 (for which there are two isoforms, Rab8A and Rab8b), IFT88 and IFT20, which are important components of the machinery of ciliary growth, were recruited normally to the ciliary base of MAL-knockdown cells but were unable to elongate the primary cilium correctly. MAL, therefore, is crucial for the proper condensation of membranes at the ciliary base, which is required for efficient primary cilium extensionThis work was supported by the Ministerio de Economıa y Competitividad, Spain [grant numbers BFU2012-32532 and CONSOLIDER COAT CSD2009-00016 to M. A.A.]. G.A. was supported by the Amarouto Program for senior researchers from the Comunidad Autónoma de Madri

The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation

The base of the primary cilium contains a zone of condensedmembranes whose importance is not known. Here, we have studiedthe involvement of MAL, a tetraspanning protein that exclusivelypartitions into condensed membrane fractions, in the condensation ofmembranes at the ciliary base and investigated the importance ofthese membranes in primary cilium formation. We show that MALaccumulates at the ciliary base of epithelial MDCK cells. Knockdownof MAL expression resulted in a drastic reduction in the condensationof membranes at the ciliary base, the percentage of ciliated cells andthe length of the cilia, but did not affect the docking of the centrosometo the plasma membrane or produce missorting of proteins to thepericentriolar zone or to the membrane of the remaining cilia. Rab8(for which there are two isoforms, Rab8A and Rab8b), IFT88 andIFT20, which are important components of the machinery of ciliarygrowth, were recruited normally to the ciliary base of MAL-knockdowncells but were unable to elongate the primary cilium correctly. MAL,therefore, is crucial for the proper condensation of membranes at theciliary base, which is required for efficient primary cilium extension

Fortalecimiento Socio-Organizativo en pequeñas poblaciones. La Aguadita. Departamento Capital de la Provincia de Catamarca

En este trabajo se describe el fortalecimiento social para la organización de una localidad con identidad cultural histórico-industrial, con potencial turístico, y  de la población para el desarrollo local. Esta situación solo es posible, en la vinculación de la Universidad Nacional de Catamarca en conjunto el Plan Estratégico, en el apoyo al desarrollo socio-cultural de la comunidad catamarqueña. Esta comunicación se desprende del proyecto Caracterización de los Materiales Pétreos de Edificación, que tiene el objetivo de contribuir a la utilización racional y sustentable de los recursos en actividades del geoturismo. A tal fin sociabiliza un encuentro, una acción conjunta de la Universidad, el equipo técnico del proyecto, al grupo gestor para lo cual se define en primera instancia desarrollar las capacidades de información y capacitación que necesita, así como el respectivo asesoramiento técnico para el desarrollo de productos y su conservación en itinerarios. La metodología es acorde a las necesidades del grupo meta, que se ajusta al nivel de aprendizaje del medio, se orienta a fortalecer las capacidades y potencialidades de los recursos humanos en competencia, a la capacidad de organización y de gestión  de los recursos para operar actividades turísticas, de tal forma que estos grupos se integren a la oferta del turismo urbano. Aplica herramientas de fácil comprensión, presenta experiencias locales sobre los temas a capacitar y sobre todo trabajar con actividades prácticas en donde el grupo se integra y su aprendizaje es mucho más eficaz. Dentro de aspectos metodológicos citados es importante resaltar el enfoque geológico y, turístico que deberá cumplir cada materia a impartir, ya que la formación no puede ser aislada de los objetivos del proyecto matriz. Bajo este enfoque se ha iniciado el trabajo y se tienen resultados de su primera fase, identificación de los recursos, valorización de estructuras geológicas-arquitectónicas, identificación y caracterización de los actores, diagnóstico de diferentes perspectivas e identificación de las posibles maneras de involucramiento al futuro desarrollo geoturistico. El objetivo fundamental es hacer que estos actores claves generen desde sus recursos, proyectos turísticos como ejes fundamentales y dinamizadores del desarrollo local en colaboración interinstitucional que consideren al turismo como una actividad social-cultural para el desarrollo económico de su región.

Overweight in schoolchildren and nutrient intake

Introduction: According to the World Health Organization (WHO), one of the most serious health problems of the 21st century is childhood obesity. In 2016, more than 400 million children and adolescents between the age of 5 and 19 were overweight or obese. The aim of this study is to evaluate the consumption, habits and dietary practices of schoolchildren who suffer from malnutrition due to overweight or obesity. Methods: descriptive, correlational and prospective study using a quantitative approach. The sample size was 82 girls and boys who met the inclusion criteria between 8 and 14 years of a public institution of basic education in Barranquilla. Results: schoolchildren of both sexes had a high prevalence of obesity, particularly in the female group (76.44%). A dietary interview using the frequency of food consumption method showed a moderate consumption of fruits and vegetables, as well as a high consumption of sugary drinks, fried foods, cookies and sweets, and a low intake of water. Regarding eating habits and practices, it was reported that a high percentage of schoolchildren receive diets and purchase cookies, ice cream, sugary drink or juices and salty snacks. Discussion: a a study revealed in 56 schoolchildren evaluated the prevalence of overweight of Spanish schoolchildren according to sex, thus showing a higher prevalence of overweight in girls (21.9%) than in boys, (10.1%) while the prevalence go obesity was higher in boys (24.5%) than in girls (9.7%). A systematic analysis reported in a population of children and adults from 1980 to 2013, finding a prevalence of overweight and obesity in boys (26.3% and 9.4%, respectively) and girls (27.3% and 8.3%). Our study presents excessive figures related to obesity mainly in schoolgirls (76.44%). Conclusion: Our data show a relationship between the prevalence of overweight and obesity and poor consumption and eating habits and practices

Hacer Arqueología en el contexto de Aislamiento Social Preventivo y Obligatorio

El presente artículo tiene como propósito reflexionar acerca de los aportes que la Arqueología y los arqueólogos brindan a la sociedad en el marco del Aislamiento Social Preventivo y Obligatorio que rige en nuestro país como consecuencia de la pandemia de COVID-19. Se presenta la diversidad de tareas que han realizado los arqueólogos en estos tiempos y se analizan las múltiples demandas que han surgido por parte de distintos sectores de la sociedad respecto del quehacer de esta disciplina. Se propone que el rol de la Arqueología en estos tiempos es aportar contenidos en el ámbito de la cultura y de la educación en todos sus nivelesFil: Lanzelotti, Sonia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Saavedra 15. Instituto de las Culturas. Universidad de Buenos Aires. Instituto de las Culturas; ArgentinaFil: Chichkoyan, Karina Vanesa. Universidad Nacional de Luján. Departamento de Ciencias Sociales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Acuña Suárez, Gabriel E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano. Universidad Nacional del Centro de la Provincia de Buenos Aires. Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano; ArgentinaFil: Aranda, Claudia Marcela. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Estevez, Juan Manuel. Universidad de Buenos Aires. Facultad de Filosofía y Letras. Museo Etnográfico "Juan B. Ambrosetti"; ArgentinaFil: Favier Dubois, Cristian Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; ArgentinaFil: Guarnochea, Miguel. Universidad Nacional de Luján. Departamento de Ciencias Sociales; ArgentinaFil: Lamami, Marcelo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Saavedra 15. Instituto de las Culturas. Universidad de Buenos Aires. Instituto de las Culturas; ArgentinaFil: Luna, Leandro Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Saavedra 15. Instituto Multidisciplinario de Historia y Ciencias Humanas; ArgentinaFil: Martínez, Lila. Universidad Nacional de Lujan. Rectorado. Instituto de Investigaciones Geograficas; ArgentinaFil: Maruscak, Gala. Universidad de Buenos Aires. Facultad de Filosofía y Letras; ArgentinaFil: Saiz Reales, Mauro. Universidad de Buenos Aires. Facultad de Filosofía y Letras; Argentin

First-in-human PeriCord cardiac bioimplant : scalability and GMP manufacturing of an allogeneic engineered tissue graft

Altres ajuts: La Marató de TV3 Foundation, Government of Catalonia, Catalan Society of Cardiology, "La Caixa" Banking Foundation, Spanish Ministry of Science, Innovation and Universities, Institute of Health Carlos III, and the European Regional Development Fund.Small cardiac tissue engineering constructs show promise for limiting post-infarct sequelae in animal models. This study sought to scale-up a 2-cm 2 preclinical construct into a human-size advanced therapy medicinal product (ATMP; PeriCord), and to test it in a first-in-human implantation. The PeriCord is a clinical-size (12-16 cm 2) decellularised pericardial matrix colonised with human viable Wharton's jelly-derived mesenchymal stromal cells (WJ-MSCs). WJ-MSCs expanded following good manufacturing practices (GMP) met safety and quality standards regarding the number of cumulative population doublings, genomic stability, and sterility. Human decellularised pericardial scaffolds were tested for DNA content, matrix stiffness, pore size, and absence of microbiological growth. PeriCord implantation was surgically performed on a large non-revascularisable scar in the inferior wall of a 63-year-old male patient. Coronary artery bypass grafting was concomitantly performed in the non-infarcted area. At implantation, the 16-cm 2 pericardial scaffold contained 12·5 × 10 6 viable WJ-MSCs (85·4% cell viability; <0·51 endotoxin units (EU)/mL). Intraoperative PeriCord delivery was expeditious, and secured with surgical glue. The post-operative course showed non-adverse reaction to the PeriCord, without requiring host immunosuppression. The three-month clinical follow-up was uneventful, and three-month cardiac magnetic resonance imaging showed ~9% reduction in scar mass in the treated area. This preliminary report describes the development of a scalable clinical-size allogeneic PeriCord cardiac bioimplant, and its first-in-human implantation. La Marató de TV3 Foundation, Government of Catalonia, Catalan Society of Cardiology, "La Caixa" Banking Foundation, Spanish Ministry of Science, Innovation and Universities, Institute of Health Carlos III, and the European Regional Development Fund

Search for new particles in events with energetic jets and large missing transverse momentum in proton-proton collisions at root s=13 TeV

A search is presented for new particles produced at the LHC in proton-proton collisions at root s = 13 TeV, using events with energetic jets and large missing transverse momentum. The analysis is based on a data sample corresponding to an integrated luminosity of 101 fb(-1), collected in 2017-2018 with the CMS detector. Machine learning techniques are used to define separate categories for events with narrow jets from initial-state radiation and events with large-radius jets consistent with a hadronic decay of a W or Z boson. A statistical combination is made with an earlier search based on a data sample of 36 fb(-1), collected in 2016. No significant excess of events is observed with respect to the standard model background expectation determined from control samples in data. The results are interpreted in terms of limits on the branching fraction of an invisible decay of the Higgs boson, as well as constraints on simplified models of dark matter, on first-generation scalar leptoquarks decaying to quarks and neutrinos, and on models with large extra dimensions. Several of the new limits, specifically for spin-1 dark matter mediators, pseudoscalar mediators, colored mediators, and leptoquarks, are the most restrictive to date.Peer reviewe