Gil, C.

Hansen, Rasmus

Hernáez, M. Luisa

Martínez-Gomariz, Montserrat

Martínez-Solano, Laura

Molero, Gloria

Reales-Calderón, José Antonio

Repositorio Institucional de la Universidad de Córdoba

Posters S6. Animal, Plant and Microbial Proteomics165P. 93DIVING INTO THE MOUSE MACROPHAGE CYTOSKELETAL PROTEOME UPON THE INTERACTION WITH CANDiDA ALBiCANSJose Antonio Reales-Calderón1, Laura Martínez-Solano1, Montserrat Martínez-Gomariz2, María Luisa Hernáez2, Rasmus Hansen2, Gloria Molero1 and Concha Gil1,21Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid; 2Unidad de Proteómica, Parque Científico de Madrid, Universidad Complutense de MadridHost-pathogen studies open interesting opportunities for the search of new virulence determinants and new targets for antimicrobial therapies. To study the host response, we have chosen the murine macrophage cell line RAW 264.7, in the light of the importance of macrophages for optimal host protection against C. albicans systemic infections. We have performed a sub-proteomic study about the induced expression/repression of proteins from macrophages when they are in contact with C. albicans, based on DIGE.RAW 264.7 cells were allowed to interact with C. albicans SC 5314 cells for 3h, and an important differential protein expression was observed in these macrophages compared to control ones in the 4 fractions analysed: organelle, cytoplasm, nucleus and cytoskeleton. Of 120 spots with differential protein expression, 21 proteins have been identified. Of these 120 spots, 70 were in cytoskeletal fraction, but only 3 spots were identified. The same sample was analyzed on LTQ mass spectrometer and the identifications showed that most of the proteins in this fraction were neither cytoskeletal nor related proteins. Thus, other cytoskeletal extraction protocols have been assayed, highly increasing the number of cytoskeletal proteins identified. This will allow us the study of the cytoskeletal proteins changes in macrophages during the interaction with Candida albicans using iTRAQ technology.

Diving into the mouse macrophage cytoskeletal proteome upon the interaction with candida albicans

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