Pennsylvania Folklife Vol. 31, No. 4

• Of Baskets and Basket Makers • Egg Decorating at the Kutztown Folk Festival • Spatterware • Scrimshaw • Folk Musical Instruments at the Kutztown Folk Festival • Puppets: Fun at the Festival • Festival Focus • Folk Festival Programs • Festival Focus on Quilts • The Kutztown Folk Festival\u27s Calico Seamstresses • Summer Drinks of the Pennsylvania Dutch • The Folk Festival\u27s Lace Maker • The Country Cemetery: Connection Between Past and Present • Coopering • The Dialect of the Pennsylvania Dutchhttps://digitalcommons.ursinus.edu/pafolklifemag/1096/thumbnail.jp

Checkpoint-Dependent and -Independent Roles of Swi3 in Replication Fork Recovery and Sister Chromatid Cohesion in Fission Yeast

Multiple genome maintenance processes are coordinated at the replication fork to preserve genomic integrity. How eukaryotic cells accomplish such a coordination is unknown. Swi1 and Swi3 form the replication fork protection complex and are involved in various processes including stabilization of replication forks, activation of the Cds1 checkpoint kinase and establishment of sister chromatid cohesion in fission yeast. However, the mechanisms by which the Swi1–Swi3 complex achieves and coordinates these tasks are not well understood. Here, we describe the identification of separation-of-function mutants of Swi3, aimed at dissecting the molecular pathways that require Swi1–Swi3. Unlike swi3 deletion mutants, the separation-of-function mutants were not sensitive to agents that stall replication forks. However, they were highly sensitive to camptothecin that induces replication fork breakage. In addition, these mutants were defective in replication fork regeneration and sister chromatid cohesion. Interestingly, unlike swi3-deleted cell, the separation-of-functions mutants were proficient in the activation of the replication checkpoint, but their fork regeneration defects were more severe than those of checkpoint mutants including cds1Δ, chk1Δ and rad3Δ. These results suggest that, while Swi3 mediates full activation of the replication checkpoint in response to stalled replication forks, Swi3 activates a checkpoint-independent pathway to facilitate recovery of collapsed replication forks and the establishment of sister chromatid cohesion. Thus, our separation-of-function alleles provide new insight into understanding the multiple roles of Swi1-Swi3 in fork protection during DNA replication, and into understanding how replication forks are maintained in response to different genotoxic agents

Exogenously induced expression of ethylene biosynthesis, ethylene perception, phospholipase D, and Rboh-oxidase genes in broccoli seedlings

In higher plants, copper ions, hydrogen peroxide, and cycloheximide have been recognized as very effective inducers of the transcriptional activity of genes encoding the enzymes of the ethylene biosynthesis pathway. In this report, the transcriptional patterns of genes encoding the 1-aminocyclopropane-1-carboxylate synthases (ACSs), 1-aminocyclopropane-1-carboxylate oxidases (ACOs), ETR1, ETR2, and ERS1 ethylene receptors, phospholipase D (PLD)-α1, -α2, -γ1, and -δ, and respiratory burst oxidase homologue (Rboh)-NADPH oxidase-D and -F in response to these inducers in Brassica oleracea etiolated seedlings are shown. ACS1, ACO1, ETR2, PLD-γ1, and RbohD represent genes whose expression was considerably affected by all of the inducers used. The investigations were performed on the seedlings with (i) ethylene insensitivity and (ii) a reduced level of the PLD-derived phosphatidic acid (PA). The general conclusion is that the expression of ACS1, -3, -4, -5, -7, and -11, ACO1, ETR1, ERS1, and ETR2, PLD-γ 1, and RbohD and F genes is undoubtedly under the reciprocal cross-talk of the ethylene and PAPLD signalling routes; both signals affect it in concerted or opposite ways depending on the gene or the type of stimuli. The results of these studies on broccoli seedlings are in agreement with the hypothesis that PA may directly affect the ethylene signal transduction pathway via an inhibitory effect on CTR1 (constitutive triple response 1) activity

Incorporating a prognostic representation of marine nitrogen fixers into the global ocean biogeochemical model HAMOCC

Nitrogen (N2) fixation is a major source of bioavailable nitrogen to the euphotic zone, thereby exerting an important control on ocean biogeochemical cycling. This paper presents the incorporation of prognostic N2 fixers into the HAMburg Ocean Carbon Cycle model (HAMOCC), a component of the Max Planck Institute Earth System Model (MPI‐ESM). Growth dynamics of N2 fixers in the model are based on physiological characteristics of the cyanobacterium Trichodesmium. The applied temperature dependency confines diazotrophic growth and N2 fixation to the tropical and subtropical ocean roughly between 40°S and 40°N. Simulated large‐scale spatial patterns compare well with observations, and the global N2 fixation rate of 135.6 Tg N yr−1 is within the range of current estimates. The vertical distribution of N2 fixation also matches well the observations, with a major fraction of about 85% occurring in the upper 20 m. The observed seasonal variability at the stations BATS and ALOHA is reasonably reproduced, with highest fixation rates in northern summer/fall. Iron limitation was found to be an important factor in controlling the simulated distribution of N2 fixation, especially in the Pacific Ocean. The new model component considerably improves the representation of present‐day N2 fixation in HAMOCC. It provides the basis for further studies on the role of diazotrophs in global biogeochemical cycles, as well as on the response of N2 fixation to changing environmental conditions

Systems biology analysis of temporal in vivo Brucella melitensis and bovine transcriptomes predicts host:pathogen protein-protein interactions

To date, fewer than 200 gene-products have been identified as Brucella virulence factors, and most were characterized individually without considering how they are temporally and coordinately expressed or secreted during the infection process. Here, we describe and analyze the in vivo temporal transcriptional profile of Brucella melitensis during the initial 4 h interaction with cattle. Pathway analysis revealed an activation of the “Two component system” providing evidence that the in vivo Brucella sense and actively regulate their metabolism through the transition to an intracellular lifestyle. Contrarily, other Brucella pathways involved in virulence such as “ABC transporters” and “T4SS system” were repressed suggesting a silencing strategy to avoid stimulation of the host innate immune response very early in the infection process. Also, three flagellum-encoded loci (BMEII0150-0168, BMEII1080-1089, and BMEII1105-1114), the “flagellar assembly” pathway and the cell components “bacterial-type flagellum hook” and “bacterial-type flagellum” were repressed in the tissue-associated B. melitensis, while RopE1 sigma factor, a flagellar repressor, was activated throughout the experiment. These results support the idea that Brucella employ a stealthy strategy at the onset of the infection of susceptible hosts. Further, through systems-level in silico host:pathogen protein–protein interactions simulation and correlation of pathogen gene expression with the host gene perturbations, we identified unanticipated interactions such as VirB11::MAPK8IP1; BtaE::NFKBIA, and 22 kDa OMP precursor::BAD and MAP2K3. These findings are suggestive of new virulence factors and mechanisms responsible for Brucella evasion of the host's protective immune response and the capability to maintain a dormant state. The predicted protein–protein interactions and the points of disruption provide novel insights that will stimulate advanced hypothesis-driven approaches toward revealing a clearer understanding of new virulence factors and mechanisms influencing the pathogenesis of brucellosis.To date, fewer than 200 gene-products have been identified as Brucella virulence factors, and most were characterized individually without considering how they are temporally and coordinately expressed or secreted during the infection process. Here, we describe and analyze the in vivo temporal transcriptional profile of Brucella melitensis during the initial 4 h interaction with cattle. Pathway analysis revealed an activation of the "Two component system" providing evidence that the in vivo Brucella sense and actively regulate their metabolism through the transition to an intracellular lifestyle. Contrarily, other Brucella pathways involved in virulence such as "ABC transporters" and "T4SS system" were repressed suggesting a silencing strategy to avoid stimulation of the host innate immune response very early in the infection process. Also, three flagellum-encoded loci (BMEII0150-0168, BMEII1080-1089, and BMEII1105-1114), the "flagellar assembly" pathway and the cell components "bacterial-type flagellum hook" and "bacterial-type flagellum" were repressed in the tissue-associated B. melitensis, while RopE1 sigma factor, a flagellar repressor, was activated throughout the experiment. These results support the idea that Brucella employ a stealthy strategy at the onset of the infection of susceptible hosts. Further, through systems-level in silico host:pathogen protein-protein interactions simulation and correlation of pathogen gene expression with the host gene perturbations, we identified unanticipated interactions such as VirB11Peer reviewedVeterinary Pathobiolog

A review of zoonotic infection risks associated with the wild meat trade in Malaysia.

The overhunting of wildlife for food and commercial gain presents a major threat to biodiversity in tropical forests and poses health risks to humans from contact with wild animals. Using a recent survey of wildlife offered at wild meat markets in Malaysia as a basis, we review the literature to determine the potential zoonotic infection risks from hunting, butchering and consuming the species offered. We also determine which taxa potentially host the highest number of pathogens and discuss the significant disease risks from traded wildlife, considering how cultural practices influence zoonotic transmission. We identify 51 zoonotic pathogens (16 viruses, 19 bacteria and 16 parasites) potentially hosted by wildlife and describe the human health risks. The Suidae and the Cervidae families potentially host the highest number of pathogens. We conclude that there are substantial gaps in our knowledge of zoonotic pathogens and recommend performing microbial food safety risk assessments to assess the hazards of wild meat consumption. Overall, there may be considerable zoonotic risks to people involved in the hunting, butchering or consumption of wild meat in Southeast Asia, and these should be considered in public health strategies

COVID-19 symptoms at hospital admission vary with age and sex: results from the ISARIC prospective multinational observational study

Background: The ISARIC prospective multinational observational study is the largest cohort of hospitalized patients with COVID-19. We present relationships of age, sex, and nationality to presenting symptoms. Methods: International, prospective observational study of 60 109 hospitalized symptomatic patients with laboratory-confirmed COVID-19 recruited from 43 countries between 30 January and 3 August 2020. Logistic regression was performed to evaluate relationships of age and sex to published COVID-19 case definitions and the most commonly reported symptoms. Results: ‘Typical’ symptoms of fever (69%), cough (68%) and shortness of breath (66%) were the most commonly reported. 92% of patients experienced at least one of these. Prevalence of typical symptoms was greatest in 30- to 60-year-olds (respectively 80, 79, 69%; at least one 95%). They were reported less frequently in children (≤ 18 years: 69, 48, 23; 85%), older adults (≥ 70 years: 61, 62, 65; 90%), and women (66, 66, 64; 90%; vs. men 71, 70, 67; 93%, each P < 0.001). The most common atypical presentations under 60 years of age were nausea and vomiting and abdominal pain, and over 60 years was confusion. Regression models showed significant differences in symptoms with sex, age and country. Interpretation: This international collaboration has allowed us to report reliable symptom data from the largest cohort of patients admitted to hospital with COVID-19. Adults over 60 and children admitted to hospital with COVID-19 are less likely to present with typical symptoms. Nausea and vomiting are common atypical presentations under 30 years. Confusion is a frequent atypical presentation of COVID-19 in adults over 60 years. Women are less likely to experience typical symptoms than men

Tilpasset opplæring i samfunnsfag : en kvalitativ studie av samfunnsfagslæreres oppfatning av tilpasset opplæring i praktisk klasseromsarbeid

Tema for denne studien er tilpasset opplæring i Samfunnsfag for Vg1/Vg2. Eksisterende forskning har i stor grad konkludert med at tilpasset opplæring er for dårlig i dagens skole, men hvordan er dette i Samfunnsfag? Studien skal belyse følgende problemstilling: Hvordan forholder et utvalg samfunnsfagslærere seg til tilpasset opplæring? Underlagt denne problemstillingen er følgende forskningsspørsmål: 1. Hvordan tolker samfunnsfaglærere begrepet tilpasset opplæring? 2. Hva mener lærerne om mulighetene for å gjennomføre tilpasning i Samfunnsfag? Hensikten med denne studien er å få et bilde av hvordan samfunnsfagslærere forholder seg til begrepet og hvilke tanker de har om tilpasning i egen praksis. Teorigrunnlaget omhandler eksisterende forskning og litteratur om tilpasset opplæring, samt Kunnskapsløftet og Utdanningsdirektoratets veiledninger. Metoden som er benyttet i denne studien er kvalitative forskningsintervjuer av fem samfunnsfagslærere ved ulike videregående skoler i Oslo. Dataene, som funnene er basert på, er transkriberte intervjuer. Studiens funn omhandler begrepet tilpasset opplæring, medbestemmelse og tilpasning i Samfunnsfag. Det må nevnes at det er interne forskjeller mellom lærerne. Allikevel tyder det på at tilpasset opplæring er et uklart begrep for de fleste av lærerne, samtidig som flere nevner at begrepet ikke brukes. Studien skiller mellom lærernes teoretiske og praktiske forståelse av begrepet. Lærerne tolker begrepet noe ulikt. Dette fører antakelig til noe ulik praksis. Samtlige lærere er positive til medbestemmelse, samtidig som de tar hensyn til modningsnivå og læringsutbytte når undervisningen planlegges. Balansen mellom lærerens autoritet og medbestemmelse er viktig. Manglende tid er sentralt med tanke på tilpasning i Samfunnsfag, og mange av lærerne mener dette har konsekvenser for tilpasningen. Det kan også se ut som behovet for tilpasning er mindre sammenlignet med andre fag. Med andre ord forholder samfunnsfagslærerne seg i relativt liten grad til tilpasset opplæring, selv om aspekter ved tilpasning er viktige momenter for lærerne

Võtke minust eesmärki! Monikielinen lapsi rakenteita yhdistämässä

Our aim is to discuss language strategies of a trilingual child, who uses Estonian, Finnish and Norwegian on a daily basis. Especially, we focus on the influence of the Finnish language on the Estonian language used by our informant. Estonian and Finnish are closely related languages, and they both have rich morphological system. We argue that the interaction between morphologically rich inflectional systems is diff erent than the interaction between morphologically very different systems. Our informant speaks Estonian with her mother and brother and Norwegian with her father. She lives in Finland, and Finnish is her school language as well as the language of many daily situations. At the time of collecting the data, she was 10 years old