FOXP2 expression in frontotemporal lobar degeneration-tau

FOXP2 is altered in a variety of language disorders. We found reduced mRNA and protein expression of FOXP2 in frontal cortex area 8 in Pick's disease, and frontotemporal lobar degeneration-tau linked to P301L mutation presenting with language impairment in comparison with age-matched controls and cases with parkinsonian variant progressive supranuclear palsy. Foxp2 mRNA and protein are also reduced with disease progression in the somatosensory cortex in transgenic mice bearing the P301S mutation in MAPT when compared with wild-type littermates. Our findings support the presence of FOXP2 expression abnormalities in sporadic and familial frontotemporal degeneration tauopathies

SEROVARS AND ANTIMICROBIAL RESISTANCE OF Salmonella spp. ISOLATED FROM TURKEY AND BROILER CARCASSES IN SOUTHERN BRAZIL BETWEEN 2004 AND 2006

Salmonella spp. causes diseases in fowls, when species-specific serovars (Salmonella Pullorum and S.Gallinarum) are present in flocks, and public health problems, when non-typhoid serovars are isolated, as well as possible bacterial resistance induced by the preventive and therapeutic use of antimicrobials in animal production. This study describes the serovars and bacterial resistance of 280Salmonella spp. strains isolated from turkey and broiler carcasses in Southern Brazil between 2004 and 2006. SalmonellaEnteritidis was the most prevalent serovar (55.7%), followed by Heidelberg (5.0%), Agona (4.3%), Bredeney (3.9%), Hadar (3.2%), and Typhimurium (2.9%). Tennessee and S. Enterica subspecies enterica(O: 4.5) were isolated only in turkeys, and Hadar (18.6%) was the most prevalent serovar in this species. Antimicrobial susceptibility tests were performed in 178 isolates (43 from turkeys and 135 from broilers). All isolates were sensitive to amoxicillin + clavulanic acid, polymyxin B, ciprofloxacin, and norfloxacin, and were resistant to bacitracin and penicillin. Broiler carcass isolates showed resistance to nalidixic acid (48.9%), nitrofurantoin (34.3%), neomycin (9.6%), tetracycline (5.2%), and kanamycin (8.9%); and turkey carcass isolates were resistant to nalidixic acid (62.8%), tetracycline (34.9%), and neomycin (30.2%), with a significant difference in turkeys when compared to broiler carcass isolates. These results indicate the need for judicious use of antimicrobials in livestock production, given that the serovars identified are potential causes of food poisoning

Socio-Environmental and Hematological Profile of Landfill Residents (Sao Jorge Landfill-Sao Paulo, Brazil)

We are experiencing an unprecedented urbanization process that, alongside physical, social and economic developments, has been having a significant impact on a population's health. Due to the increase in pollution, violence and poverty, our modern cities no longer ensure a good quality of life so they become unhealthy environments. This study aims to assess the effect of social, environmental and economic factors on the hematologic profile of residents of Santo Andre's landfill. In particular, we will assess the effect of social, economic, and environmental factors on current and potential disease markers obtained from hematological tests. The research method is the observational type, from a retrospective cohort, and by convenience sampling in Santo Andre in the Greater ABC (municipalities of Santo Andre, Sao Bernardo do Campo and Sao Caetano do Sul, southeast part of the Greater Sao Paulo Metropolitan Area, Brazil). The study determined a socio-environmental profile and the hematologic diseases screening related to a close location to the landfill. The disease manifests itself within a broad spectrum of symptoms that causes changes in blood count parameters. The objective of this work is to show that there is an association between social, environmental and economic factors and a variety of serious disease outcomes that may be detected from blood screening. A causal study of the effect of living near the landfill on these disease outcomes would be a very expensive and time-consuming study. This work we believe is sufficient for public health officials to consider policy and attempt remediation of the effects of living near a landfill.ABC Medical SchoolSchool of Health Technology Coimbra-IPC-Polytechnic Institute of Coimbra-PortugalDepartment of Environmental Health and Public HealthSEMASA-Sanitation and Insurance CentralABC MedSch, Environm Hlth Management Dept, BR-09060650 Santo Andre, SP, BrazilUniv Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Dept Biol Sci, BR-04060650 Diadema, SP, BrazilCoimbra Hlth Sch, EsTesC, Dept Environm Hlth Sci, P-3046854 Coimbra, PortugalBiological Sciences Department, Institute of Environmental, Chemistry and Pharmaceutical Sciences, Universidade Federal de São Paulo (UNIFESP), Diadema 04060-650, SP, BrazilWeb of Scienc

OBEDIS Core Variables Project : European Expert Guidelines on a Minimal Core Set of Variables to Include in Randomized, Controlled Clinical Trials of Obesity Interventions

Heterogeneity of interindividual and intraindividual responses to interventions is often observed in randomized, controlled trials for obesity. To address the global epidemic of obesity and move toward more personalized treatment regimens, the global research community must come together to identify factors that may drive these heterogeneous responses to interventions. This project, called OBEDIS (OBEsity Diverse Interventions Sharing - focusing on dietary and other interventions), provides a set of European guidelines for a minimal set of variables to include in future clinical trials on obesity, regardless of the specific endpoints. Broad adoption of these guidelines will enable researchers to harmonize and merge data from multiple intervention studies, allowing stratification of patients according to precise phenotyping criteria which are measured using standardized methods. In this way, studies across Europe may be pooled for better prediction of individuals' responses to an intervention for obesity - ultimately leading to better patient care and improved obesity outcomes.Peer reviewe

O Partido do Brasil: uma história do Partido do Movimento Democrático Brasileiro (1980/2016)

A presente tese tem como objetivo analisar a representação político partidária no Brasil, com ênfase na história do Brasil recente e, mais especificamente, na história do Partido do Movimento democrático Brasileiro (PMDB). Discutiremos ao longo do trabalho a relação do partido com o Estado brasileiro (na “Nova República”), seu lugar de classe, seus programas, seus intelectuais, suas representações nos estados da federação, bem como seu papel central de viabilização de maiorias que possibilitaram os governos na Nova República. Nossas problemáticas giram em torno das seguintes questões: por que o PMDB se constituiu no maior partido político brasileiro, mesmo não exercendo a Presidência da República, e por que os diferentes governos só foram viáveis a partir do apoio do referido partidoThe present thesis aims to analyze party political representation in Brazil, with emphasis on recent Brazilian history and more specifically on the history of the Partido do Movimento Democrático Brasileiro (PMDB). We´ll discuss the relationship of the party with Brazilian state (in the Nova República), it´s class place, it´s programs, it´s intellectuals, it´s representatios in the states of the federations, as well as it´s central role of enabling majorities that madde possible the governments in the Nova República. Our problems revolve around the following questions: why o PMDN was constituted in the largest Brazilian political party, even thought it did not wold the presidency of the republic, and why the different governments were only viable from the support of the said party300 f

Saúde como desenvolvimento: perspectivas para atuação do BNDES no complexo industrial da saúde

Bibliografia: p. 329-332A saúde é uma das áreas de maior convergência entre aspectos sociais e econômicos do desenvolvimento, pois condiciona o pleno exercício dos direitos humanos e demanda uma complexa cadeia de bens e serviços de alta tecnologia. Nos países em desenvolvimento, as transições epidemiológica e demográfica apontam para um crescimento acelerado do mercado e a aproximação de suas necessidades de saúde às dos países desenvolvidos. Dessa forma, a construção de uma base industrial em saúde passou ao centro das agendas nacionais de desenvolvimento. no Brasil, o maior desafio do Complexo Industrial da saúde é ampliar os investimentos em inovação, como forma de expandir sua competitividade. o objetivo deste trabalho é discutir o papel do BNDES em um contexto em que promover a inovação constitui meio para ampliar o acesso da população brasileira a novos produtos de saúde.Health promotion is a basic condition for human rights fullfilment and requires a complex chain of high technology goods and services, being a key area of both social and economic development. In developing countries, the demographic and epidemiological transitions points out to an intense growth of the market and to a convergence of their health needs to those of developed countries. Thus, building a health care industrial base became a central issue in national development political agendas. In Brazil, the greatest challenge for the Health Industry is increasing innovation investments, in order to compete in the global market. In this context, we discuss the role of BNDES in fostering innovation as means to expand Brazilian people’s access to new health products

Characteristics of the equine embryo and fetus from days 15 to 107 of pregnancy

In spite of numerous, substantial advances in equine reproduction, many stages of embryonic and fetal morphological development are poorly understood, with no apparent single source of comprehensive information. Hence, the objective of the present study was to provide a complete macroscopic and microscopic description of the equine embryo/fetus at various gestational ages. Thirty-four embryos/fetuses were aged based on their crown rump length (CRL), and submitted to macroscopic description, biometry, light and scanning microscopy, as well as the alizarin technique. All observed developmental changes were chronologically ordered and described. As examples of the main observed features, an accentuated cervical curvature was observed upon macroscopic examination in all specimens. In the nervous system, the encephalic fourth ventricle and the encephalic vesicles forebrain, midbrain, and hindbrain, were visualized from Day 19 (ovulation = Day 0). The thoracic and pelvic limbs were also visualized; their extremities gave rise to the hoof during development from Day 27. Development of other structures such as pigmented optical vesicle, liver, tail, cardiac area, lungs, and dermal vascularization started on Days 25, 25, 19, 19, 34, and 35, respectively. Light and scanning microscopy facilitated detailed examinations of several organs, e.g., heart, kidneys, lungs, and intestine, whereas the alizarin technique enabled visualization of ossification. Observations in this study contributed to the knowledge regarding equine embryogenesis, and included much detailed data from many specimens collected over a long developmental interval. (C) 2011 Elsevier Inc. All rights reserved.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[2008/54851-3

Medicinal properties of Angelica archangelica root extract: cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development

Although Angelica archangelica is a medicinal and aromatic plant with a long history of use for both medicinal and food purposes, there are no studies regarding the antineoplastic activity of its root. This study aimed to evaluate the cytotoxicity and antitumor effects of the crude extract of A. archangelica root (CEAA) on breast cancer. The cytotoxicity of CEAA against breast adenocarcinoma cells (4T1 and MCF-7) was evaluated by a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Morphological and biochemical changes were detected by Hoechst 33342/propidium iodide (PI) and annexin V/PI staining. Cytosolic calcium mobilization was evaluated in cells staining with FURA-4NW. Immunoblotting was used to determine the effect of CEAA on anti- and pro-apoptotic proteins (Bcl-2 and Bax, respectively). The 4T1 cell-challenged mice were used for in vivo assay. Using ultra-high-performance liquid chromatography-mass spectrometry analysis, angelicin, a constituent of the roots and leaves of A. archangelica, was found to be the major constituent of the CEAA evaluated in this study (73 mg/mL). The CEAA was cytotoxic for both breast cancer cell lines studied but not for human fibroblasts. Treatment of 4T1 cells with the CEAA increased Bax protein levels accompanied by decreased Bcl-2 expression, in the presence of cleaved caspase-3 and cytosolic calcium mobilization, suggesting mitochondrial involvement in breast cancer cell death induced by the CEAA in this cell line. No changes on the Bcl-2/Bax ratio were observed in CEAA-treated MCF7 cells. Gavage administration of the CEAA (500 mg/kg) to 4T1 cell-challenged mice significantly decreased tumor growth when compared with untreated animals. Altogether, our data show the antitumor potential of the CEAA against breast cancer cells in vitro and in vivo. Further research is necessary to better elucidate the pharmacological application of the CEAA in breast cancer therapy172132140CAPES - Coordenação de Aperfeiçoamento de Pessoal e Nível SuperiorCNPQ - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPESP – Fundação de Amparo à Pesquisa Do Estado De São PauloSem informaçãoSem informação2008/58035-

Association of the SOD2 Polymorphism (Val16Ala) and SOD Activity with Vaso-occlusive Crisis and Acute Splenic Sequestration in Children with Sickle Cell Anemia

Submitted by Paulo Silva ([email protected]) on 2019-11-18T13:17:51Z No. of bitstreams: 1 Association of the SOD2 Polymorphism (Val16Ala) and SOD Activity with Vaso-occlusive Crisis and Acute Splenic Sequestration in Children with Sickle Cell Anemia.pdf: 335007 bytes, checksum: a614766bb4931821c7294ae8bbbed5e6 (MD5)Approved for entry into archive by Paulo Silva ([email protected]) on 2019-11-18T13:55:11Z (GMT) No. of bitstreams: 1 Association of the SOD2 Polymorphism (Val16Ala) and SOD Activity with Vaso-occlusive Crisis and Acute Splenic Sequestration in Children with Sickle Cell Anemia.pdf: 335007 bytes, checksum: a614766bb4931821c7294ae8bbbed5e6 (MD5)Made available in DSpace on 2019-11-18T13:55:11Z (GMT). No. of bitstreams: 1 Association of the SOD2 Polymorphism (Val16Ala) and SOD Activity with Vaso-occlusive Crisis and Acute Splenic Sequestration in Children with Sickle Cell Anemia.pdf: 335007 bytes, checksum: a614766bb4931821c7294ae8bbbed5e6 (MD5) Previous issue date: 2018FACEPEUniversidade de Pernambuco. Instituto de Ciências Biológicas. Recife, PE, Brasil.Universidade de Pernambuco. Instituto de Ciências Biológicas. Recife, PE, Brasil / Universidade Federal Rural de Pernambuco. Programa de Pós-Graduação em Biotecnologia. Recife, PE, Brasil.Universidade de Pernambuco. Instituto de Ciências Biológicas. Recife, PE, Brasil.Universidade de Pernambuco. Instituto de Ciências Biológicas. Recife, PE, Brasil.Universidade Federal de Pernambuco. Recife, PE, Brasil.Universidade de Pernambuco. Instituto de Ciências Biológicas. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Universidade Federal de Pernambuco. Recife, PE, Brasil / Fundação de Hematologia e Hemoterapia de Pernambuco. Recife, PE, Brasil.Fundação de Hematologia e Hemoterapia de Pernambuco. Recife, PE, Brasil.Universidade de Pernambuco. Instituto de Ciências Biológicas. Recife, PE, Brasil.Universidade Federal de Pernambuco. Recife, PE, Brasil.Fundação de Hematologia e Hemoterapia de Pernambuco. Recife, PE, Brasil.Universidade de Pernambuco. Instituto de Ciências Biológicas. Recife, PE, Brasil / Fundação de Hematologia e Hemoterapia de Pernambuco. Recife, PE, Brasil.The SOD2 polymorphism Val16Ala T→C influences the antioxidative response. This study investigated the association of the SOD2 polymorphism and superoxide dismutase (SOD) activity with the vaso-occlusive crisis (VOC) and acute splenic sequestration (ASS) in children with sickle cell anemia (SCA). One hundred ninety-five children with SCA aged 1-9 years old were analyzed. The TC and CC genotypes were associated with lower SOD activity compared with the TT genotype (p=0.0321; p=0.0253, respectively). Furthermore, TC and CC were more frequent in patients with VOC or ASS (p=0.0285; p=0.0090, respectively). These results suggest that the SOD2 polymorphism associated with low SOD activity could be a susceptibility factor for the occurrence of VOC and ASS