432 research outputs found
Quantitation of angiogenesis in vitro induced by VEGF-A and FGF-2 in two different human endothelial cultures : an all-in-one assay
Angiogenic therapy is considered to be a promising tool for treatment of ischemic diseases. Many in vivo and in vitro
assays have been developed to identify potential proangiogenic drugs and to investigate their mode of action. However, until now no validated system exists that would allow quantitation of angiogenesis in vitro in only one assay. Here, a previously established all-in-one in vitro assay based on staging of the angiogenic cascade was validated by quantitation of the effects of the known proangiogenic factors VEGF-A and FGF-2. Both growth factors were applied separately or in combination to human endothelial cell cultures derived from the heart and the foreskin, and angiogenesis was quantitated over 30 days of culture. Additionally, gene expression of VEGFR-1, VEGFR-2 and FGFR-1 at 3, 10, 20 or 40 days of cultivation was quantitated by RT-qPCR. In both cultures, VEGF-A as well as FGF-2 induced a run through all defined stages of angiogenesis in vitro. Application of VEGF-A only led to formation of irregular globular endothelial structures, while FGF-2 resulted in development of regular capillary-like structures. Quantitation of the angiogenic effects of VEGF-A and transcripts of VEGFR-1 and VEGFR-2 showed that a high VEGFR-1/VEGFR-2 ratio evoked deceleration of angiogenesis
Size-dependent decoherence of excitonic states in semiconductor microcrystallites
The size-dependent decoherence of the exciton states resulting from the
spontaneous emission is investigated in a semiconductor spherical
microcrystallite under condition . In general, the
larger size of the microcrystallite corresponds to the shorter coherence time.
If the initial state is a superposition of two different excitonic coherent
states, the coherence time depends on both the overlap of two excitonic
coherent states and the size of the microcrystallite. When the system with
fixed size is initially in the even or odd coherent states, the larger average
number of the excitons corresponds to the faster decoherence. When the average
number of the excitons is given, the bigger size of the microcrystallite
corresponds to the faster decoherence. The decoherence of the exciton states
for the materials GaAs and CdS is numerically studied by our theoretical
analysis.Comment: 4 pages, two figure
On quantum teleportation with beam-splitter-generated entanglement
Following the lead of Cochrane, Milburn, and Munro [Phys. Rev. A {\bf 62},
062307 (2000)], we investigate theoretically quantum teleportation by means of
the number-sum and phase-difference variables. We study Fock-state entanglement
generated by a beam splitter and show that two-mode Fock-state inputs can be
entangled by a beam splitter into close approximations of maximally entangled
eigenstates of the phase difference and the photon-number sum
(Einstein-Podolsky-Rosen -- EPR -- states). Such states could be experimentally
feasible with on-demand single-photon sources. We show that the teleportation
fidelity can reach near unity when such ``quasi-EPR'' states are used as the
quantum channel.Comment: 7 pages (two-column), 7 figures, submitted to Phys. Rev. A. Text
unmodified, postscript error correcte
Neoantigen screening identifies broad TP53 mutant immunogenicity in patients with epithelial cancers.
Emergence of quasi-metallic state in disordered 2D electron gas due to strong interactions
The interrelation between disorder and interactions in two dimensional
electron liquid is studied beyond weak coupling perturbation theory. Strong
repulsion significantly reduces the electronic density of states on the Fermi
level. This makes the electron liquid more rigid and strongly suppresses
elastic scattering off impurities. As a result the weak localization, although
ultimately present at zero temperature and infinite sample size, is
unobservable at experimentally accessible temperature at high enough densities.
Therefore practically there exists a well defined metallic state. We study
diffusion of electrons in this state and find that the diffusion pole is
significantly modified due to "mixture" with static photons similar to the
Anderson - Higgs mechanism in superconductivity. As a result several effects
stemming from the long range nature of diffusion like the Aronov - Altshuler
logarithmic corrections to conductivity are less pronounced.Comment: to appear in Phys. Rev.
A power-splitting relaying protocol for wireless energy harvesting and information processing in NOMA systems
Non-orthogonal multiple access (NOMA) along with cooperative communications have been recognized as promising candidates for the fifth generation (5G) wireless networks and have attracted many researchers. Every networked device however has its own limited power supply. To this extent, this paper investigates a power-splitting relaying (PSR) protocol for wireless energy harvesting and information processing in the NOMA systems to prolong the lifetime of the energy-constrained relay nodes in wireless networks so as to avail the ambient radio-frequency (RF) signal as well as to simultaneously harvest the energy and process the information. Decode-and-forward relaying is employed at the relay node where the energy from the received RF signal is harvested and exploited to forward the information to the destination. Specifically, the outage probability and ergodic rate of the PSR protocol are derived to realize the impacts of energy harvesting time, energy harvesting efficiency, power splitting ratio, source data rate, and the distance between nodes. It is also shown that an increased energy harvesting efficiency results in an enhanced performance and an outperformance in terms of the energy efficiency is achieved with the employment of the NOMA when compared to the conventional orthogonal multiple access. Numerical results are provided to verify the findings
Tight-binding parameters for charge transfer along DNA
We systematically examine all the tight-binding parameters pertinent to
charge transfer along DNA. The molecular structure of the four DNA bases
(adenine, thymine, cytosine, and guanine) is investigated by using the linear
combination of atomic orbitals method with a recently introduced
parametrization. The HOMO and LUMO wavefunctions and energies of DNA bases are
discussed and then used for calculating the corresponding wavefunctions of the
two B-DNA base-pairs (adenine-thymine and guanine-cytosine). The obtained HOMO
and LUMO energies of the bases are in good agreement with available
experimental values. Our results are then used for estimating the complete set
of charge transfer parameters between neighboring bases and also between
successive base-pairs, considering all possible combinations between them, for
both electrons and holes. The calculated microscopic quantities can be used in
mesoscopic theoretical models of electron or hole transfer along the DNA double
helix, as they provide the necessary parameters for a tight-binding
phenomenological description based on the molecular overlap. We find that
usually the hopping parameters for holes are higher in magnitude compared to
the ones for electrons, which probably indicates that hole transport along DNA
is more favorable than electron transport. Our findings are also compared with
existing calculations from first principles.Comment: 15 pages, 3 figures, 7 table
Search for a W' boson decaying to a bottom quark and a top quark in pp collisions at sqrt(s) = 7 TeV
Results are presented from a search for a W' boson using a dataset
corresponding to 5.0 inverse femtobarns of integrated luminosity collected
during 2011 by the CMS experiment at the LHC in pp collisions at sqrt(s)=7 TeV.
The W' boson is modeled as a heavy W boson, but different scenarios for the
couplings to fermions are considered, involving both left-handed and
right-handed chiral projections of the fermions, as well as an arbitrary
mixture of the two. The search is performed in the decay channel W' to t b,
leading to a final state signature with a single lepton (e, mu), missing
transverse energy, and jets, at least one of which is tagged as a b-jet. A W'
boson that couples to fermions with the same coupling constant as the W, but to
the right-handed rather than left-handed chiral projections, is excluded for
masses below 1.85 TeV at the 95% confidence level. For the first time using LHC
data, constraints on the W' gauge coupling for a set of left- and right-handed
coupling combinations have been placed. These results represent a significant
improvement over previously published limits.Comment: Submitted to Physics Letters B. Replaced with version publishe
Search for the standard model Higgs boson decaying into two photons in pp collisions at sqrt(s)=7 TeV
A search for a Higgs boson decaying into two photons is described. The
analysis is performed using a dataset recorded by the CMS experiment at the LHC
from pp collisions at a centre-of-mass energy of 7 TeV, which corresponds to an
integrated luminosity of 4.8 inverse femtobarns. Limits are set on the cross
section of the standard model Higgs boson decaying to two photons. The expected
exclusion limit at 95% confidence level is between 1.4 and 2.4 times the
standard model cross section in the mass range between 110 and 150 GeV. The
analysis of the data excludes, at 95% confidence level, the standard model
Higgs boson decaying into two photons in the mass range 128 to 132 GeV. The
largest excess of events above the expected standard model background is
observed for a Higgs boson mass hypothesis of 124 GeV with a local significance
of 3.1 sigma. The global significance of observing an excess with a local
significance greater than 3.1 sigma anywhere in the search range 110-150 GeV is
estimated to be 1.8 sigma. More data are required to ascertain the origin of
this excess.Comment: Submitted to Physics Letters
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