Molecular characterization of group A rotaviruses in Mukuru slums Kenya: detection of novel strains circulating in children below 5 years of age

Abstract Background Gastroenteritis is a public health concern due to high morbidity and mortality among children. Rotaviruses are the leading etiological agents of severe gastroenteritis in children and accounts for more than half a million deaths per year in Africa. The study aimed at investigating the rotavirus genotypes that were circulating in children aged 5 years and below in and around Mukuru slums in Nairobi County Kenya. Methods A purposive cross sectional sampling method was applied where 166 samples were collected from children below 5 years of age and taken to Kenya Medical Research Institute virology laboratory. Presence of rotaviruses was determined using reverse transcription polymerase chain reaction, while extraction was done using ZR Soil/Fecal RNA MicroPrep™ extraction kit. This was followed by reverse transcription and genotyping using various group A rotavirus primers. Results The G type was successfully determined in 37 (92.5%), while the P type was successfully determined in 35 (87.5%) of the 40 (24%) page positive samples. Type G1 was the most predominant of the G types (40.5%), and the incidences of G3 and G9 were 21.6 and 32.4% respectively. Mixed types G3/G9 were detected at 5.4%. Three P types existed in Mukuru slums, P[8] (60%), P[6] (22.9%), P[4] (11.4) and their relative incidence varied over the 15 months of this study. Conclusions The G types and P types detected in this study are important causes of acute gastroenteritis in Mukuru slums Nairobi Kenya. An indication that the prevalence of certain genotypes may change over a rotavirus season is significant and mirrors observations from studies in other tropical climates. Thus monitoring of the genotypic changes among circulating viruses should be encouraged over the coming years

Restocking of small water bodies for a post Covid recovery and growth of fisheries and aquaculture production:socioeconomic implications

Funding: The International Fund for Agricultural Development (IFAD) and The Government of Kenya through the Aquaculture Business Development Programme (ABDP) funded the restocking and data collection and processing.Restocking of fish in Small Water Bodies (SWBs) is one of the technologies that can be used to enhance fish-food production for post Covid recovery and growth in food security, and national development. The current study aimed at assessing the socioeconomic impact and stock performance of restocked Nile tilapia fingerlings in SWBs in 15 counties in the Western and Central regions where the Aquaculture Business Development Programme (ABDP) is implemented. The study employed both primary and secondary data from socioeconomics, environmental characteristics and fisheries and aquaculture aspects. There was no restocked dam with a low (< 1.66) socioeconomic impact, indicating the potential for restocking. The majority (n = 27; 79%) of the restocked SWBs had a moderate (1.66 – 2.33) impact, owing to the inherent constraints of adoptability by the local community. Twenty one percent (n = 7; 21%) of the SWBs had a high (2.34 - 3.00) impact and with better environmental conditions. The average condition factor (K) of tilapia in restocked SWBs was 1.24 ± 0.53 SD, suggesting excellent fish growth condition. Notably, restocking the SWBs could benefit riparian fishing communities by improving their livelihoods and providing food and nutritional security. Given the limited exploitation of fish in most SWBs in the developing countries, additional community awareness and capacity building interventions are needed to enhance optimal use of SWBs in post Covid era.Publisher PDFPeer reviewe

Biomarker-confirmed suboptimal adherence to isoniazid preventive therapy among children with HIV in western Kenya

Objectives:The aim of this study was to assess the level and correlates of biomarker-confirmed adherence to isoniazid (INH) preventive therapy (IPT) among children with HIV (CLHIV).Design:This prospective cohort study assessed adherence among CLHIV on IPT in public sector HIV clinics from 2019 through 2020.Methods:Adherence was assessed by pill counts or caregiver or self-reports, and urine biomarkers (in-house dipstick and Isoscreen). Both urine biomarker tests detect INH metabolites within 48 h of ingestion. Consistent adherence was defined as having positive results on either biomarker at all visits. Correlates of biomarker-confirmed nonadherence at each visit were evaluated using generalized estimating equations. The in-house dipstick was validated using Isoscreen as the reference.Results:Among 97 CLHIV on IPT with adherence assessments, median age was 10 years (IQR 7-13). All were on ART at IPT initiation (median duration 46 months [IQR 4-89]); 81% were virally suppressed (<1000 copies/ml). At all visits, 59% (n = 57) of CLHIV reported taking at least 80% of their doses, while 39% (n = 38) had biomarker-confirmed adherence. Viral nonsuppression (adjusted risk ratio [aRR] = 1.65; 95% confidence interval [95% CI] 1.09-2.49) and the sixth month of IPT use (aRR = 2.49; 95% CI 1.34-4.65) were independent correlates of biomarker-confirmed nonadherence at each visit. Sensitivity and specificity of the in-house dipstick were 98.1% (94.7-99.6%) and 94.7% (88.1-98.3%), respectively, versus Isoscreen.Conclusion:Biomarker-confirmed adherence to IPT was sub-optimal and was associated with viral nonsuppression and duration of IPT. Urine dipstick testing may be useful in assessing adherence to IPT in clinical care