Amphibia, Anura, Leptodactylidae, <i>Leptodactylus cupreus</i> Caramaschi, Feio and Sao-Pedro, 2008: Distribution extension

We provide the first record of Leptodactylus cupreus out of its type locality, as well the first record of this speciesin the State of Espírito Santo, southeastern Brazil. The record of Leptodactylus cupreus from municipality Santa Teresaextends in approximately 220 km to the northeastern of its geographic distribution and establishes the inferior limit of 800m in its altitudinal distribution in the Serra da Mantiqueira

Sample size effects on the assessment of eukaryotic diversity and community structure in aquatic sediments using high-throughput sequencing

Understanding how biodiversity changes in time and space is vital to assess the effects of environmental change on benthic ecosystems. Due to the limitations of morphological methods, there has been a rapid expansion in the application of high-throughput sequencing methods to study benthic eukaryotic communities. However, the effect of sample size and small-scale spatial variation on the assessment of benthic eukaryotic diversity is still not well understood. Here, we investigate the effect of different sample volumes in the genetic assessment of benthic metazoan and non-metazoan eukaryotic community composition. Accordingly, DNA was extracted from five different cumulative sediment volumes comprising 100% of the top 2 cm of five benthic sampling cores, and used as template for Ilumina MiSeq sequencing of 18 S rRNA amplicons. Sample volumes strongly impacted diversity metrics for both metazoans and non-metazoan eukaryotes. Beta-diversity of treatments using smaller sample volumes was significantly different from the beta-diversity of the 100% sampled area. Overall our findings indicate that sample volumes of 0.2 g (1% of the sampled area) are insufficient to account for spatial heterogeneity at small spatial scales, and that relatively large percentages of sediment core samples are needed for obtaining robust diversity measurement of both metazoan and non-metazoan eukaryotes

High throughput shotgun sequencing of eRNA reveals taxonomic and derived functional shifts across a benthic productivity gradient

Benthic macrofauna is regularly used in monitoring programmes, however the vast majority of benthic eukaryotic biodiversity lies mostly in microscopic organisms, such as meiofauna (invertebrates <1 mm) and protists, that rapidly responds to environmental change. These communities have traditionally been hard to sample and handle in the laboratory, but DNA sequencing has made such work less time consuming. While DNA sequencing captures both alive and dead organisms, environmental RNA (eRNA) better targets living organisms or organisms of recent origin in the environment. Here, we assessed the biodiversity of three known bioindicator microeukaryote groups (nematodes, foraminifera, and ciliates) in sediment samples collected at seven coastal sites along an organic carbon (OC) gradient. We aimed to investigate if eRNA shotgun sequencing can be used to simultaneously detect differences in (i) biodiversity of multiple microeukaryotic communities; and (ii) functional feeding traits of nematodes. Results showed that biodiversity was lower for nematodes and foraminifera in high OC (6.2%-6.9%), when compared to low OC sediments (1.2%-2.8%). Dissimilarity in community composition increased for all three groups between Low OC and High OC, as well as the classified feeding type of nematode genera (with more nonselective deposit feeders in high OC sediment). High relative abundant genera included nematodeSabatieriaand foraminiferaElphidiumin high OC, andCryptocaryon-like ciliates in low OC sediments. Considering that future sequencing technologies are likely to decrease in cost, the use of eRNA shotgun sequencing to assess biodiversity of benthic microeukaryotes could be a powerful tool in recurring monitoring programmes.Peer reviewe

O ORDENAMENTO COERCITIVO DE HANS KELSEN: UMA ANÁLISE (CRÍTICA) ACERCA DA CENTRALIDADE DA SANÇÃO

Estudar clássicos como a Teoria Pura do Direito de Hans Kelsen é de suma importância para entender, ao menos minimamente, a história e a origem do Direito. Não se trata de uma teoria ultrapassada, mas sim uma das mais (senão a mais) importante teoria do direito já desenvolvida. O caráter coercitivo de um ordenamento jurídico encontra seu respaldo através da sanção, elemento central da estática jurídica kelseniana. Isto posto, o presente estudo objetiva estudar especialmente as concepções de estática jurídica propostas por Hans Kelsen, no tocante à centralidade da sanção, bem como as críticas de outros autores impostas a essa particularidade. Nesse sentido, questiona-se: em que medida o Direito pode ter a sansão como questão central da estática do ordenamento jurídico e quais são possíveis substituições para essa centralidade? Por certo é notória a existência de normas que estatuam sanções em qualquer ordenamento jurídico, mas trazer a sanção para o centro da estática do Direito é, de certa forma, um conceito insuficiente para explicar a complexidade de um ordenamento jurídico. Dessa forma, as teoria propostas por autores como Norberto Bobbio, Herbert Hart, assim como Pontes de Miranda, se mostram mais adequadas e menos radicais no tocante ao assunto estudado

Above-below surface interactions mediate effects of seagrass disturbance on meiobenthic diversity, nematode and polychaete trophic structure

Ecological interactions between aquatic plants and sediment communities can shape the structure and function of natural systems. Currently, we do not fully understand how seagrass habitat degradation impacts the biodiversity of belowground sediment communities. Here, we evaluated indirect effects of disturbance of seagrass meadows on meiobenthic community composition, with a five-month in situ experiment in a tropical seagrass meadow. Disturbance was created by reducing light availability (two levels of shading), and by mimicking grazing events (two levels) to assess impacts on meiobenthic diversity using high-throughput sequencing of 18S rRNA amplicons. Both shading and simulated grazing had an effect on meiobenthic community structure, mediated by seagrass-associated biotic drivers and sediment abiotic variables. Additionally, shading substantially altered the trophic structure of the nematode community. Our findings show that degradation of seagrass meadows can alter benthic community structure in coastal areas with potential impacts to ecosystem functions mediated by meiobenthos in marine sediments

Aleitamento materno, alimentação complementar, sobrepeso e obesidade em pré-escolares

OBJECTIVE: To analyze the association of overweight and obesity with breastfeeding and complementary feeding in pre-school children. METHODS: Cross-sectional study with 566 children, enrolled in private schools of the city of São Paulo, Southeastern Brazil, in 2004-2005. The dependent variable was overweight and obesity. Body Mass Index percentile curves were employed to classify children's nutritional status, considering values e"P85 andOBJETIVO: Analizar la asociación del sobrepeso y de la obesidad con el amamantamiento materno y la alimentación complementaria en preescolares. MÉTODOS: Estudio transversal envolviendo 566 niños matriculados en escuelas privadas en el municipio de Sao Paulo (Sureste de Brasil), 2004-2005. La variable dependiente fue sobrepeso y obesidad. Para la clasificación del estado nutricional de los niños fueron utilizadas las curvas de percentiles del índice de masa corporal para edad, clasificando como sobrepeso valores ?P85 yOBJETIVO: Analisar a associação do sobrepeso e da obesidade com o aleitamento materno e a alimentação complementar em pré-escolares. MÉTODOS: Estudo transversal envolvendo 566 crianças matriculadas em escolas particulares no município de São Paulo, SP, 2004-2005. A variável dependente foi sobrepeso e obesidade. Para a classificação do estado nutricional das crianças foram utilizadas as curvas de percentis do Índice de Massa Corporal para idade, classificando como sobrepeso valores e"P85

Rotation, activity, and lithium abundance in cool binary stars

We have used two robotic telescopes to obtain time-series high-resolution spectroscopy and V I and/or by photometry for a sample of 60 active stars. Orbital solutions are presented for 26 SB2 and 19 SB1 systems with unprecedented phase coverage and accuracy. The total of 6,609 R=55,000 echelle spectra are also used to systematically determine effective temperatures, gravities, metallicities, rotational velocities, lithium abundances and absolute H{\alpha}-core fluxes as a function of time. The photometry is used to infer unspotted brightness, V - I and/or b - y colors, spot-induced brightness amplitudes and precise rotation periods. Our data are complemented by literature data and are used to determine rotation-temperature-activity relations for active binary components. We also relate lithium abundance to rotation and surface temperature. We find that 74% of all known rapidly-rotating active binary stars are synchronized and in circular orbits but 26% are rotating asynchronously of which half have Prot > Porb and e > 0. Because rotational synchronization is predicted to occur before orbital circularization active binaries should undergo an extra spin-down besides tidal dissipation. We suspect this to be due to a magnetically channeled wind with its subsequent braking torque. We find a steep increase of rotation period with decreasing effective temperature for active stars. For inactive, single giants with Prot > 100 d, the relation is much weaker. Our data also indicate a period-activity relation for H{\alpha} of the form RH{\alpha} \propto P - 0.24 for binaries and RH{\alpha} \propto P -0.14 for singles. Lithium abundances in our sample increase with effective temperature. On average, binaries of comparable effective temperature appear to exhibit 0.25 dex less surface lithium than singles. We also find a trend of increased Li abundance with rotational period of form log n(Li) \propto - 0.6 log Prot

Real-world evaluation of the impact of statin intensity on adherence and persistence to therapy : a Scottish population-based study

Aim: To assess associations between statin intensity and adherence, persistence and discontinuation of statin therapy in Scotland. Method: Retrospective cohort study, using linked electronic health records covering a period from January 2009 to December 2016. The study cohort included adult patients (≥18 years) newly initiating statins within Greater Glasgow and Clyde, Scotland. Study outcomes comprised adherence, discontinuation and persistence to treatment, stratified by three exposure groups (high, moderate and low intensity). Discontinuation and persistence were calculated using the refill-gap and anniversary methods, respectively. Proportion of days covered (PDC) was used as a proxy for adherence. Kaplan-Meier survival curves and Cox proportional hazard models were used to evaluate discontinuation, and associations between adherence/persistence and statin intensity were assessed using logistic regression. Results: A total of 73 716 patients with a mean age of 61.4 ± 12.6 years were included; the majority (88.3%) received moderate intensity statins. Discontinuation rates differed between intensity levels, with high-intensity patients less likely to discontinue treatment compared to those on moderate intensity (prior cardiovascular disease [CVD]: HR 0.43 [95% CI 0.34-0.55]; no prior CVD: 0.80 [0.74-0.86]). Persistence declined over time, and high-intensity patients had the highest persistence rates. Overall, 52.6% of patients were adherent to treatment (PDC ≥ 80%), but adherence was considerably higher among high-intensity patients (63.7%). Conclusion: High-intensity statins were associated with better persistence and adherence to treatment, but overall long-term persistence and adherence remain a challenge, particularly among patients without prior CVD. This needs addressing