Interactive effects of vanadium and phosphorus on their uptake, growth and heat shock proteins in chickpea genotypes under hydroponic conditions

The present study was carried out to examine the interaction of vanadium and phosphorus and changes in heat shock genes to optimize the growth of chickpea genotypes. Two sets of hydroponic experiments were carried out using vanadium and phosphorus with five-level central composite design. Five levels of vanadium (0-1180 mu M) and phosphorus (0-100011 mu M) were used to evaluate their interactive effects. Plants fresh biomass and uptake of vanadium and phosphorus were influenced by vanadium and phosphorus application. Enhanced fresh biomass was most likely a result of increased phosphorus uptake by chickpea genotypes. Addition of vanadium induced toxic effects while, higher concentration of phosphorus alleviated its toxic effects. The obtained results also indicated that lower vanadium concentration promoted phosphorus absorption however; higher concentration of vanadium inhibited the phosphorus uptake. The morphological changes in leaves indicated that the cells were deformed and reduced in size when treated with higher vanadium levels with fixed phosphorus while, there was little deformation and reduction in cells size were observed when plants were treated with higher levels of phosphorus with fixed vanadium. Whereas, the proportion of deformation of cells were higher in Balkasar as compared to C-44 genotype. The results also showed that at elevated vanadium with fixed phosphorus, Hsp70 was expressed only in C-44 while, not in Balkasar however, Hsp90 and GAPDH showed non-significant results. (C) 2016 Elsevier B.V. All rights reserved

Global burden of chronic respiratory diseases and risk factors, 1990–2019: an update from the Global Burden of Disease Study 2019

Background: Updated data on chronic respiratory diseases (CRDs) are vital in their prevention, control, and treatment in the path to achieving the third UN Sustainable Development Goals (SDGs), a one-third reduction in premature mortality from non-communicable diseases by 2030. We provided global, regional, and national estimates of the burden of CRDs and their attributable risks from 1990 to 2019. Methods: Using data from the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2019, we estimated mortality, years lived with disability, years of life lost, disability-adjusted life years (DALYs), prevalence, and incidence of CRDs, i.e. chronic obstructive pulmonary disease (COPD), asthma, pneumoconiosis, interstitial lung disease and pulmonary sarcoidosis, and other CRDs, from 1990 to 2019 by sex, age, region, and Socio-demographic Index (SDI) in 204 countries and territories. Deaths and DALYs from CRDs attributable to each risk factor were estimated according to relative risks, risk exposure, and the theoretical minimum risk exposure level input. Findings: In 2019, CRDs were the third leading cause of death responsible for 4.0 million deaths (95% uncertainty interval 3.6–4.3) with a prevalence of 454.6 million cases (417.4–499.1) globally. While the total deaths and prevalence of CRDs have increased by 28.5% and 39.8%, the age-standardised rates have dropped by 41.7% and 16.9% from 1990 to 2019, respectively. COPD, with 212.3 million (200.4–225.1) prevalent cases, was the primary cause of deaths from CRDs, accounting for 3.3 million (2.9–3.6) deaths. With 262.4 million (224.1–309.5) prevalent cases, asthma had the highest prevalence among CRDs. The age-standardised rates of all burden measures of COPD, asthma, and pneumoconiosis have reduced globally from 1990 to 2019. Nevertheless, the age-standardised rates of incidence and prevalence of interstitial lung disease and pulmonary sarcoidosis have increased throughout this period. Low- and low-middle SDI countries had the highest age-standardised death and DALYs rates while the high SDI quintile had the highest prevalence rate of CRDs. The highest deaths and DALYs from CRDs were attributed to smoking globally, followed by air pollution and occupational risks. Non-optimal temperature and high body-mass index were additional risk factors for COPD and asthma, respectively. Interpretation: Albeit the age-standardised prevalence, death, and DALYs rates of CRDs have decreased, they still cause a substantial burden and deaths worldwide. The high death and DALYs rates in low and low-middle SDI countries highlights the urgent need for improved preventive, diagnostic, and therapeutic measures. Global strategies for tobacco control, enhancing air quality, reducing occupational hazards, and fostering clean cooking fuels are crucial steps in reducing the burden of CRDs, especially in low- and lower-middle income countries

Global burden of 369 diseases and injuries in 204 countries and territories, 1990–2019: a systematic analysis for the Global Burden of Disease Study 2019

Background: In an era of shifting global agendas and expanded emphasis on non-communicable diseases and injuries along with communicable diseases, sound evidence on trends by cause at the national level is essential. The Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) provides a systematic scientific assessment of published, publicly available, and contributed data on incidence, prevalence, and mortality for a mutually exclusive and collectively exhaustive list of diseases and injuries. Methods: GBD estimates incidence, prevalence, mortality, years of life lost (YLLs), years lived with disability (YLDs), and disability-adjusted life-years (DALYs) due to 369 diseases and injuries, for two sexes, and for 204 countries and territories. Input data were extracted from censuses, household surveys, civil registration and vital statistics, disease registries, health service use, air pollution monitors, satellite imaging, disease notifications, and other sources. Cause-specific death rates and cause fractions were calculated using the Cause of Death Ensemble model and spatiotemporal Gaussian process regression. Cause-specific deaths were adjusted to match the total all-cause deaths calculated as part of the GBD population, fertility, and mortality estimates. Deaths were multiplied by standard life expectancy at each age to calculate YLLs. A Bayesian meta-regression modelling tool, DisMod-MR 2.1, was used to ensure consistency between incidence, prevalence, remission, excess mortality, and cause-specific mortality for most causes. Prevalence estimates were multiplied by disability weights for mutually exclusive sequelae of diseases and injuries to calculate YLDs. We considered results in the context of the Socio-demographic Index (SDI), a composite indicator of income per capita, years of schooling, and fertility rate in females younger than 25 years. Uncertainty intervals (UIs) were generated for every metric using the 25th and 975th ordered 1000 draw values of the posterior distribution. Findings: Global health has steadily improved over the past 30 years as measured by age-standardised DALY rates. After taking into account population growth and ageing, the absolute number of DALYs has remained stable. Since 2010, the pace of decline in global age-standardised DALY rates has accelerated in age groups younger than 50 years compared with the 1990–2010 time period, with the greatest annualised rate of decline occurring in the 0–9-year age group. Six infectious diseases were among the top ten causes of DALYs in children younger than 10 years in 2019: lower respiratory infections (ranked second), diarrhoeal diseases (third), malaria (fifth), meningitis (sixth), whooping cough (ninth), and sexually transmitted infections (which, in this age group, is fully accounted for by congenital syphilis; ranked tenth). In adolescents aged 10–24 years, three injury causes were among the top causes of DALYs: road injuries (ranked first), self-harm (third), and interpersonal violence (fifth). Five of the causes that were in the top ten for ages 10–24 years were also in the top ten in the 25–49-year age group: road injuries (ranked first), HIV/AIDS (second), low back pain (fourth), headache disorders (fifth), and depressive disorders (sixth). In 2019, ischaemic heart disease and stroke were the top-ranked causes of DALYs in both the 50–74-year and 75-years-and-older age groups. Since 1990, there has been a marked shift towards a greater proportion of burden due to YLDs from non-communicable diseases and injuries. In 2019, there were 11 countries where non-communicable disease and injury YLDs constituted more than half of all disease burden. Decreases in age-standardised DALY rates have accelerated over the past decade in countries at the lower end of the SDI range, while improvements have started to stagnate or even reverse in countries with higher SDI. Interpretation: As disability becomes an increasingly large component of disease burden and a larger component of health expenditure, greater research and developm nt investment is needed to identify new, more effective intervention strategies. With a rapidly ageing global population, the demands on health services to deal with disabling outcomes, which increase with age, will require policy makers to anticipate these changes. The mix of universal and more geographically specific influences on health reinforces the need for regular reporting on population health in detail and by underlying cause to help decision makers to identify success stories of disease control to emulate, as well as opportunities to improve. Funding: Bill & Melinda Gates Foundation. © 2020 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY 4.0 licens

Ovarian Follicular Dynamics around Estrus in Beetal and Teddy Goats

Due to higher fecundity rate over Beetal goats, it is assumed that Teddy goats have higher ovarian activity. Therefore, the current study tested the hypothesis that ovarian follicular population i.e. number of small (2-4 mm), medium (>4-6 mm) and large follicles (>6 mm), size of the ovulatory follicle, and ovulation rate (No. of CL/No of ovulated goats), using transrectal ultrasonography are different between Beetal and Teddy goats of Pakistani origin. Beetal (n=6) and Teddy (n=8) does were synchronized using double PGF2a injections 10 d apart and were scanned on Days -2, 0 (estrus) and +2. The onset of estrus was assessed by aproned bucks. Mean number of small follicles were higher (P<0.05) in Beetal goats, compared to Teddy goats, on days -2, estrus and +2. The ovulatory follicle diameter, and ovulation rate were found non-significant between Beetal and Teddy goats. It is concluded that Beetal goats have greater population of small follicles around estrus compared to Teddy goats. Future studies can be based on the follicle recruitment and atretic factors in Beetal goats to enhance the production

Activity and Anti-Aflatoxigenic Effect of Indigenously Characterized Probiotic Lactobacilli against <i>Aspergillus flavus</i>—A Common Poultry Feed Contaminant

Aflatoxin contamination in human food and animal feed is a threat to public safety. Aflatoxin B1 (AFB1) can be especially damaging to poultry production and consequently economic development of Pakistan. The present study assessed the in vitro binding of AFB1 by indigenously characterized probiotic lactobacilli. Six isolates (Lactobacillus gallinarum PDP 10, Lactobacillus reuetri FYP 38, Lactobacillus fermentum PDP 24, Lactobacillus gallinarum PL 53, Lactobacillus paracasei PL 120, and Lactobacillus gallinarum PL 149) were tested for activity against toxigenic Aspergillus flavus W-7.1 (AFB1 producer) by well diffusion assay. Only three isolates (PL 53, PL 120, and PL 149) had activity against A. flavus W-7.1. The ameliorative effect of these probiotic isolates on AFB1 production was determined by co-culturing fungus with lactobacilli for 12 days, followed by aflatoxin quantification by high-performance liquid chromatography. In vitro AFB1 binding capacities of lactobacilli were determined by their incubation with a standard amount of AFB1 in phosphate buffer saline at 37 &#176;C for 2 h. AFB1 binding capacities of isolates ranged from 28&#8211;65%. Four isolates (PDP 10, PDP 24, PL 120, and PL 149) also ceased aflatoxin production completely, whereas PL 53 showed 55% reduction in AFB1 production as compared to control. The present study demonstrated Lactobacillus gallinarum PL 149 to be an effective candidate AFB1 binding agent against Aspergillus flavus. These findings further support the binding ability of lactic acid bacteria for dietary contaminants

Probing the physicochemical and sensorial properties of pasteurized milk via nanoparticles

The present study was conducted to evaluate the effects of storage time and temperature on physicochemical and sensory properties and to measure the spoilage of pasteurized fluid milk stored at different temperatures via nanoparticles on pasteurized fluid milk. The mean values for pH of milk samples decreased (from 6.81 to 6, 5.46, and 4.37), acidity increased (10%, 21%, and 35%), fat contents decreased (13%, 24%, and 32%), protein contents decreased (7%, 18%, and 24%), lactose contents decreased (5%, 7%, and 12%), and total free fatty acids increased (14%, 19%, and 27%) respectively at 4°C, 7°C, and 10°C. Similarly, the score of sensory parameters like aroma, taste, and overall acceptability decreased 16%, 23%, and 45%; 31%, 43%, and 52%; and 33%, 42%, and 53% respectively in linear pattern along with increasing the time and temperature. The L* values were non-significantly changed or decreased with mean values of 64.82 ± 0.08 to 64.13 ± 0.07 at 4°C and significantly with 64.81 ± 0.03 to 63.87 ± 0.07 and 64.77 ± 0.02 to 61.43 ± 0.05 at 7°C and 10°C respectively at 0–19th day of milk storage. During storage, the pasteurized milk stored at all temperatures 4°C, 7°C, and 10°C showed that a* value non-significantly decreased from 48.69 ± 0.18 to 48.63 ± 0.18, 48.74 ± 0.14 to 48.57 ± 0.11, and 48.68 ± 0.18 to 47.97 ± 0.07 respectively. Values of b* decreased non-significantly at 4°C and 7°C with the values of −17.56 ± 0.23 to −18.58 and −17.54 ± 0.25 to −19.85 ± 0.85 but significantly decreased at 10°C from −17.56 ± 0.23 to −43.22 ± 0.11. The results suggested that pasteurized milk can be kept for 17–19 days at 4°C but this shelf life drastically reduced at 7°C and 10°C. Color of nanoparticles was significantly changed from pink to dark blue from 14th day of storage onwards to the end at 10°C. Conclusively, nanoparticles can be used for the rapid and at the post-spoilage measurement of pasteurized milk

Investigation of Spectral Properties of DBR-Based Photonic Crystal Structure for Optical Filter Application

In this work, the spectral properties of distributed Bragg reflector-based photonic crystal (DBR-PhC) structures were studied for the near-infrared (NIR) range. Different structural properties were varied to study their effect on the quality of the stopband and the appearance of the resonant dips in the reflection spectra of the DBR-PhC structure. The investigated structural features included the depth of PhC holes, hole radius, and number of PhC elements in the DBR structure. The 11-layered DBR structure was designed with a 2.4/1.4 refractive index contrast of alternating layers. The study aimed to achieve optical filtering properties in the DBR-PhC structure, to simplify the structural complexity of Fabry-P&eacute;rot filters by eliminating the FP cavity and upper-DBR mirror. The proposed DBR-PhC device can be used in different optical filtering and sensing applications

Investigation of Spectral Properties of DBR-Based Photonic Crystal Structure for Optical Filter Application

In this work, the spectral properties of distributed Bragg reflector-based photonic crystal (DBR-PhC) structures were studied for the near-infrared (NIR) range. Different structural properties were varied to study their effect on the quality of the stopband and the appearance of the resonant dips in the reflection spectra of the DBR-PhC structure. The investigated structural features included the depth of PhC holes, hole radius, and number of PhC elements in the DBR structure. The 11-layered DBR structure was designed with a 2.4/1.4 refractive index contrast of alternating layers. The study aimed to achieve optical filtering properties in the DBR-PhC structure, to simplify the structural complexity of Fabry-Pérot filters by eliminating the FP cavity and upper-DBR mirror. The proposed DBR-PhC device can be used in different optical filtering and sensing applications

Graphene-Based Plasmonic Metamaterial Perfect Absorber for Biosensing Applications

Graphene as a mono-atomic sheet has recently grabbed attention as a material with enormous properties. It has also been examined for enhancing absorbance in the current plasmonic structure. This has led to an increment in the sensitivity of the plasmonic sensors. In this paper, we present theoretical investigation of the novel graphene-based plasmonic metamaterial perfect absorber for biosensing applications. The simulation study performs the analysis of the novel plasmonic metamaterial absorber structure by adding coatings of graphene sheets. Each sheet of graphene enhances absorbance of the structure. In this study, we demonstrate three layers of graphene sheets lead to perfect absorbance (100%) for multiple bands in the visible and near-infrared regions. Furthermore, we also computed the sensitivity of the graphene-based proposed structure by varying the refractive index (RI) of the sensing region from 1.33–1.36 with RI change of 0.01. Proposed fabrication steps for realization of the device are also discussed

Graphene-Based Plasmonic Metamaterial Perfect Absorber for Biosensing Applications

Graphene as a mono-atomic sheet has recently grabbed attention as a material with enormous properties. It has also been examined for enhancing absorbance in the current plasmonic structure. This has led to an increment in the sensitivity of the plasmonic sensors. In this paper, we present theoretical investigation of the novel graphene-based plasmonic metamaterial perfect absorber for biosensing applications. The simulation study performs the analysis of the novel plasmonic metamaterial absorber structure by adding coatings of graphene sheets. Each sheet of graphene enhances absorbance of the structure. In this study, we demonstrate three layers of graphene sheets lead to perfect absorbance (100%) for multiple bands in the visible and near-infrared regions. Furthermore, we also computed the sensitivity of the graphene-based proposed structure by varying the refractive index (RI) of the sensing region from 1.33&ndash;1.36 with RI change of 0.01. Proposed fabrication steps for realization of the device are also discussed