The teaching of French in the French educational system: Obstacle or way to promote the plurilingualism of pupils?

Este artículo propone una reflexión en profundidad sobre las consecuencias jurídicas y pedagógicas de la política lingüística del estado que afectan en la práctica cotidiana escolar al alumnado francés. La cultura académica francesa aliada con la cultura escolar se articulan al servicio de la construcción de la ciudadanía republicana. Esta construcción ideológica, política y cultural afecta a los estudiantes que incorporan al uso de la lengua francesa particularidades propias (fonéticas, semióticas, simbólicas ...) de su entorno cultural, social o de su origen familiar. Desde una perspectiva histórica y legislativa los autores abordan la orientación de la enseñanza de la lengua francesa en el sistema educativo como un obstáculo para la integración de los grupos de alumnado que no se ajustan a los estrechos patrones linguístico-políticos que el sistema impone. Y por último, desde una perspectiva aplicada, exploran los factores que pueden desembocar en la ruptura del sistema monolingüe que, además, se articula como un sistema de segregación cultural y de exclusión clasista.This paper proposes an in-depth on the legal and educational linguistic policy of the state that affect the everyday practice school French students. The allied French academic culture to the school culture are articulated to serve the construction of republican citizenship. This ideological construction, political and cultural affects students entering the use of French particularities (phonetic, semiotic or symbolic...) of its cultural, social status or family origin. From a historical and legislative perspective the authors address the orientation of the teaching of French in the educational system as an obstacle to the integration of groups of students who do not fit the narrow linguistic-political patterns that the system imposes. And finally, from an applied perspective, explores the factors that can lead to the breakdown of monolingual system, moreover, is articulated as a cultural system of segregation and exclusion of class

Annexin A2 at the interface between F-actin and membranes enriched in phosphatidylinositol 4,5,-bisphosphate

AbstractVesicle rocketing has been used as a model system for understanding the dynamics of the membrane-associated F-actin cytoskeleton, but in many experimental systems is induced by persistent, non-physiological stimuli. Localised changes in the concentration of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) in membranes stimulate the recruitment of actin-remodelling proteins to their sites of action, regulate their activity and favour vesicle rocketing. The calcium and anionic phospholipid-binding protein annexin A2 is necessary for macropinocytic rocketing and has been shown to bind both PI(4,5)P2 and the barbed-ends of F-actin filaments. Here we show that annexin A2 localises to the comet tails which form constitutively in fibroblasts from patients with Lowe Syndrome. These fibroblasts are deficient in OCRL1, a phosphatidylinositol polyphosphate 5-phosphatase with specificity for PI(4,5)P2. We show that upon depletion of annexin A2 from these cells vesicle rocketing is reduced, and that this is also dependent upon PI(4,5)P2 formation. Annexin A2 co-localised with comet-tails induced by pervanadate and hyperosmotic shock in a basophilic cell line, and in an epithelial cell line upon activation of PKC. In vitro annexin A2 promoted comet formation in a bead-rocketing assay and was sufficient to link F-actin filaments to PI(4,5)P2 containing vesicles. These observations are consistent with a role for annexin A2 as an actin nucleator on PI(4,5)P2-enriched membranes

Loss of Endocan tumorigenic properties after alternative splicing of exon 2

<p>Abstract</p> <p>Background</p> <p>Endocan was originally described as a dermatan sulfate proteoglycan found freely circulating in the blood. Endocan expression confers tumorigenic properties to epithelial cell lines or accelerate the growth of already tumorigenic cells. This molecule is the product of a single gene composed of 3 exons. Previous data showed that endocan mRNA is subject to alternative splicing with possible generation of two protein products. In the present study we identified, and functionally characterized, the alternative spliced product of the endocan gene: the exon 2-deleted endocan, called endocanΔ2.</p> <p>Methods</p> <p>Stable, endocanΔ2-overexpressing cell lines were generated to investigate the biological activities of this new alternatively spliced product of endocan gene. Tumorigenesis was studied by inoculating endocan and endocanΔ2 expressing cell lines subcutaneously in SCID mice. Biochemical properties of endocan and endocanΔ2 were studied after production of recombinant proteins in various cell lines of human and murine origin.</p> <p>Results</p> <p>Our results showed that the exon 2 deletion impairs synthesis of the glycan chain, known to be involved in the pro-tumoral effect of endocan. EndocanΔ2 did not promote tumor formation by 293 cells implanted in the skin of severe combined immunodeficient (SCID) mice.</p> <p>Conclusion</p> <p>Our results emphasize the key role of the polypeptide sequence encoded by the exon 2 of endocan gene in tumorigenesis, and suggest that this sequence could be a target for future therapies against cancer.</p

Variant Histone H2A.Z Is Globally Localized to the Promoters of Inactive Yeast Genes and Regulates Nucleosome Positioning

H2A.Z is an evolutionary conserved histone variant involved in transcriptional regulation, antisilencing, silencing, and genome stability. The mechanism(s) by which H2A.Z regulates these various biological functions remains poorly defined, in part due to the lack of knowledge regarding its physical location along chromosomes and the bearing it has in regulating chromatin structure. Here we mapped H2A.Z across the yeast genome at an approximately 300-bp resolution, using chromatin immunoprecipitation combined with tiling microarrays. We have identified 4,862 small regions—typically one or two nucleosomes wide—decorated with H2A.Z. Those “Z loci” are predominantly found within specific nucleosomes in the promoter of inactive genes all across the genome. Furthermore, we have shown that H2A.Z can regulate nucleosome positioning at the GAL1 promoter. Within HZAD domains, the regions where H2A.Z shows an antisilencing function, H2A.Z is localized in a wider pattern, suggesting that the variant histone regulates a silencing and transcriptional activation via different mechanisms. Our data suggest that the incorporation of H2A.Z into specific promoter-bound nucleosomes configures chromatin structure to poise genes for transcriptional activation. The relevance of these findings to higher eukaryotes is discussed

Variation in Structure and Process of Care in Traumatic Brain Injury: Provider Profiles of European Neurotrauma Centers Participating in the CENTER-TBI Study.

INTRODUCTION: The strength of evidence underpinning care and treatment recommendations in traumatic brain injury (TBI) is low. Comparative effectiveness research (CER) has been proposed as a framework to provide evidence for optimal care for TBI patients. The first step in CER is to map the existing variation. The aim of current study is to quantify variation in general structural and process characteristics among centers participating in the Collaborative European NeuroTrauma Effectiveness Research in Traumatic Brain Injury (CENTER-TBI) study. METHODS: We designed a set of 11 provider profiling questionnaires with 321 questions about various aspects of TBI care, chosen based on literature and expert opinion. After pilot testing, questionnaires were disseminated to 71 centers from 20 countries participating in the CENTER-TBI study. Reliability of questionnaires was estimated by calculating a concordance rate among 5% duplicate questions. RESULTS: All 71 centers completed the questionnaires. Median concordance rate among duplicate questions was 0.85. The majority of centers were academic hospitals (n = 65, 92%), designated as a level I trauma center (n = 48, 68%) and situated in an urban location (n = 70, 99%). The availability of facilities for neuro-trauma care varied across centers; e.g. 40 (57%) had a dedicated neuro-intensive care unit (ICU), 36 (51%) had an in-hospital rehabilitation unit and the organization of the ICU was closed in 64% (n = 45) of the centers. In addition, we found wide variation in processes of care, such as the ICU admission policy and intracranial pressure monitoring policy among centers. CONCLUSION: Even among high-volume, specialized neurotrauma centers there is substantial variation in structures and processes of TBI care. This variation provides an opportunity to study effectiveness of specific aspects of TBI care and to identify best practices with CER approaches

Variation in general supportive and preventive intensive care management of traumatic brain injury: a survey in 66 neurotrauma centers participating in the Collaborative European NeuroTrauma Effectiveness Research in Traumatic Brain Injury (CENTER-TBI) study

Abstract Background General supportive and preventive measures in the intensive care management of traumatic brain injury (TBI) aim to prevent or limit secondary brain injury and optimize recovery. The aim of this survey was to assess and quantify variation in perceptions on intensive care unit (ICU) management of patients with TBI in European neurotrauma centers. Methods We performed a survey as part of the Collaborative European NeuroTrauma Effectiveness Research in Traumatic Brain Injury (CENTER-TBI) study. We analyzed 23 questions focused on: 1) circulatory and respiratory management; 2) fever control; 3) use of corticosteroids; 4) nutrition and glucose management; and 5) seizure prophylaxis and treatment. Results The survey was completed predominantly by intensivists (n = 33, 50%) and neurosurgeons (n = 23, 35%) from 66 centers (97% response rate). The most common cerebral perfusion pressure (CPP) target was > 60 mmHg (n = 39, 60%) and/or an individualized target (n = 25, 38%). To support CPP, crystalloid fluid loading (n = 60, 91%) was generally preferred over albumin (n = 15, 23%), and vasopressors (n = 63, 96%) over inotropes (n = 29, 44%). The most commonly reported target of partial pressure of carbon dioxide in arterial blood (PaCO2) was 36–40 mmHg (4.8–5.3 kPa) in case of controlled intracranial pressure (ICP) < 20 mmHg (n = 45, 69%) and PaCO2 target of 30–35 mmHg (4–4.7 kPa) in case of raised ICP (n = 40, 62%). Almost all respondents indicated to generally treat fever (n = 65, 98%) with paracetamol (n = 61, 92%) and/or external cooling (n = 49, 74%). Conventional glucose management (n = 43, 66%) was preferred over tight glycemic control (n = 18, 28%). More than half of the respondents indicated to aim for full caloric replacement within 7 days (n = 43, 66%) using enteral nutrition (n = 60, 92%). Indications for and duration of seizure prophylaxis varied, and levetiracetam was mostly reported as the agent of choice for both seizure prophylaxis (n = 32, 49%) and treatment (n = 40, 61%). Conclusions Practice preferences vary substantially regarding general supportive and preventive measures in TBI patients at ICUs of European neurotrauma centers. These results provide an opportunity for future comparative effectiveness research, since a more evidence-based uniformity in good practices in general ICU management could have a major impact on TBI outcome

Surexpression et purification d'un répresseur de l'actinophage JHJ-3

JHJ-3 est un phage qui infecte les actinomycetes du genre Saccharopolyspora. Le phage JHJ-3 a ete isole suite a une induction avec de la mitomycine C d'une souche de Saccharopolyspora hirsuta 367. Ce phage se caracterise comme etant tempere puisqu'il forme des plages troubles et lysogenise des souches de Saccharopolyspora. Pour parvenir a lysogeniser les bacteries, JHJ-3 doit posseder un gene codant pour un represseur qui assurerait I'immunite des souches lysogenes contre une surinfection par JHJ-3. Des travaux ont permis de localiser un locus genetique sur Ie genome de JHJ-3 et de determiner par I'analyse de la sequence nucleotidique qu'il coderait pour une proteine de 234 acides amines. II a aussi ete demontre que cette proteine est impliquee dans la lysogenie et qu'elle exerce une regulation analogue a celle d'un represseur sur I'activite promotrice de certaines sequences phagiques. Ce projet consistait done, a surexprimer Ie gene du represseur (jrpl) dans Escherichia coil et a purifier la proteine correspondant a ce represseur. Pour y parvenir, la sequence codante du represseur de JHJ-3 a ete inseree dans un vecteur d'expression, pTrcHisC. Ce vecteur a permis la surexpression du represseur sous forme de proteine de fusion dans E. coli. Cette proteine de fusion possede entre autre du cote N-terminal un peptide constitue de six residus histidine ayant une grande affinite iii pour Ie Ni2+ ainsi qu'un site de clivage d'une endoprotease pour eliminer ce peptide apres purification. Comme beaucoup d'autres proteines surexprimees dans E. co//', la proteine de fusion etait insoluble. Afin de rendre soluble cette proteine sous conditions non-denaturantes, deux approches ont ete envisagees. Premierement, certains parametres controlant I'expression du gene de fusion ont ete modifies et deuxiement, huit autres souches d'E. coli ont ete utilisees pour I'expression. Cependant, ces deux approches se sont averees infructueuses. La proteine de fusion a du etre solubilisee avec un agent denaturant (uree) pour etre purifiee partiellement a I'aide d'une colonne d'affinite chargee au Ni2+. Par la suite, la fraction contenant la proteine de fusion a ete dialysee de fa^on a eliminer progressivement I'agent denaturant. Ainsi, I'authenticite conformationnelle et I'activite du represseur avec ou sans peptide de fusion devront etre eventuellement verifiees

Explorer une forme littéraire et théâtrale contemporaine, déclencher le récit de soi et la réflexivité chez les professionnels de l’éducation en situation de formation à partir de la biographie langagière d’Elisabeth Mazev : Mémoire pleine, Tribulations d’une étrangère d’origine

Blanchet & Vilpoux (dir.)International audienc