Record litter size in the eurasian red squirrel (Sciurus vulgaris)

In the literature, litter size in red squirrels Sciurus vulgaris ranges between 1-6 offspring. Here we report a record litter size, in Europe, of 7 young (6 males, 1 female), marked in the nest when 10-14 days old, in a high-elevation mountain pine habitat, Lombardy Alps (Northern Italy). We discuss implications of extreme winter conditions for lifehistory strategies and possible trade-offs between high reproductive investment and short life-spa

Posidonia oceanica and Zostera marina as Potential Biomarkers of Heavy Metal Contamination in Coastal Systems

International audienc

Anti-leukemia activity of alloreactive NK cells in KIR ligand-mismatched haploidentical HSCT for pediatric patients: evaluation of the functional role of activating KIR and redefinition of inhibitory KIR specificity.

none15We analyzed 21 children with leukemia receiving haploidentical hematopoietic stem cell transplantation (haplo-HSCT) from killer immunoglobulin (Ig)-like receptors (KIR) ligand-mismatched donors. We showed that, in most transplantation patients, variable proportions of donor-derived alloreactive natural killer (NK) cells displaying anti-leukemia activity were generated and maintained even late after transplantation. This was assessed through analysis of donor KIR genotype, as well as through phenotypic and functional analyses of NK cells, both at the polyclonal and clonal level. Donor-derived KIR2DL1(+) NK cells isolated from the recipient displayed the expected capability of selectively killing C1/C1 target cells, including patient leukemia blasts. Differently, KIR2DL2/3(+) NK cells displayed poor alloreactivity against leukemia cells carrying human leukocyte antigen (HLA) alleles belonging to C2 group. Unexpectedly, this was due to recognition of C2 by KIR2DL2/3, as revealed by receptor blocking experiments and by binding assays of soluble KIR to HLA-C transfectants. Remarkably, however, C2/C2 leukemia blasts were killed by KIR2DL2/3(+) (or by NKG2A(+)) NK cells that coexpressed KIR2DS1. This could be explained by the ability of KIR2DS1 to directly recognize C2 on leukemia cells. A role of the KIR2DS2 activating receptor in leukemia cell lysis could not be demonstrated. Altogether, these results may have important clinical implications for the selection of optimal donors for haplo-HSCT.openPENDE D; MARCENARO S; FALCO M; MARTINI S; BERNARDO ME; MONTAGNA D; ROMEO E; COGNET C; MARTINETTI M; MACCARIO R; MINGARI MC; VIVIER E; MORETTA L; LOCATELLI F; MORETTA A.Pende, D; Marcenaro, S; Falco, M; Martini, S; Bernardo, Me; Montagna, Daniela; Romeo, E; Cognet, C; Martinetti, M; Maccario, R; Mingari, Mc; Vivier, E; Moretta, L; Locatelli, Franco; Moretta, A

ESVM Guideline on peripheral arterial disease

International audienc

A novel allele specific polymerase chain reaction (As-pcr) assay to detect the v1016g knockdown resistance mutation confirms its widespread presence in aedes albopictus populations from Italy

Funding Information: Funding: The study received support from the project ”Sapienza 2017” and JP received support from FCT-Fundação para a Ciência e a Tecnologia, I.P., through national funds in the framework of the project PTDC/BIA-OUT/29477/2017. Funding Information: The study received support from the project ?Sapienza 2017? and JP received support from FCT-Funda??o para a Ci?ncia e a Tecnologia, I.P., through national funds in the framework of the project PTDC/BIA-OUT/29477/2017. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.Polymerase chain reaction (PCR)-based genotyping of mutations in the voltage-sensitive sodium channel (vssc) associated with resistance to pyrethroid insecticides is widely used and represents a potential early warning and monitoring system for insecticide resistance arising in mosquito populations, which are vectors of different human pathogens. In the secondary vector Aedes albopictus—an Asian species that has invaded and colonized the whole world, including temperate regions—sequencing of domain II of the vssc gene is still needed to detect the V1016G mutation associated with pyrethroid resistance. In this study we developed and tested a novel allele-specific PCR (AS-PCR) assay to genotype the V1016G mutation in this species and applied it to the analysis of wild populations from Italy. The results confirm the high accuracy of the novel AS-PCR and highlight frequencies of the V1016G allele as >5% in most sampling sites, with peaks of 20–45% in coastal touristic sites where pyrethroid treatments are extensively implemented, mostly for mosquito nuisance reduction. The high frequency of this mutation observed in Italian Ae. albopictus populations should serve as a warning bell, advocating for increased monitoring and management of a phenomenon which risks neutralizing the only weapon today available to counteract (risks of) arbovirus outbreaks.publishersversionpublishe

Physical Condition of Molecular Gas at the Centre of NGC 1097

We have used the Xco conversion factor, Local Thermal Equilibrium and Large Velocity Gradient approximation to parametrize the cold and warm phase of the interstellar medium from five different low transitions of CO in the central 21"(kpc) of NGC 1097. We have applied a one-component model and derived a typical kinetic temperature of ~33K, and its molecular Hydrogen density and a CO column density. A two-component model results in 85% cold-to-total gas fraction with a 90K warm counterpart. Furthermore, we "resolve" the spatially unresolved single dish observations by selecting velocity channels that in an interferometric velocity map correspond to specific regions. We have selected five such regions and found that the physical properties in these regions are comparable to those derived from the full line profile. This implies that the central kpc of NGC 1097 is rather homogeneous in nature, and, although the regions are not uniquely located within the ring, the star formation along the ring is homogeneously distributed (in agreement with recent Herschel observations). We have further revised the mass inflow rate onto the Supermassive Black Hole in this prototype LINER/Sy1 galaxy and found that, accounting for the total interstellar medium and applying a careful contribution of the disc thickness and corresponding stability criterion, increases the previous estimations by a factor 10. Finally we have calculated the Xco for the centre of NGC 1097 using an independent estimation of the surface density to the CO emission, and obtained Xco=(2.8+-0.5)x10^20 cm^-2(K km s^-1)^-1 at radius 10.5" and Xco=(5.0+-0.5)x10^20 cm^-2(K km s^-1)^-1 at 7.5". With the approach and analysis described, we have demonstrated that important physical properties can be derived to a resolution beyond the single dish resolution element, however, caution is necessary for interpreting the results.(Abriged)Comment: 10 pages, 6 figures, 4 tables, Accepted for publication in MNRA

Descriptors of Posidonia oceanica meadows: Use and application

The conservation of the coastal marine environment requires the possession of information that enables the global quality of the environment to be evaluated reliably and relatively quickly. The use of biological indicators is often an appropriate method. Seagrasses in general, and Posidonia oceanica meadows in particular, are considered to be appropriate for biomonitoring because of their wide distribution, reasonable size, sedentary habit, easy collection and abundance and sensitivity to modifications of littoral zone. Reasoned management, on the scale of the whole Mediterranean basin, requires standardized methods of study, to be applied by both researchers and administrators, enabling comparable results to be obtained. This paper synthesises the existing methods applied to monitor P. oceanica meadows, identifies the most suitable techniques and suggests future research directions. From the results of a questionnaire, distributed to all the identified laboratories working on this topic, a list of the most commonly used descriptors was drawn up, together with the related research techniques (e.g. standardization, interest and limits, valuation of the results). It seems that the techniques used to study meadows are rather similar, but rarely identical, even though the various teams often refer to previously published works. This paper shows the interest of a practical guide that describes, in a standardized way, the most useful techniques enabling P. oceanica meadows to be used as an environmental descriptor. Indeed, it constitutes the first stage in the process. (c) 2005 Elsevier Ltd. All rights reserved.Peer reviewe

Earthquake-reactivated landslide scenarios in Southern Italy based on spectral-matching input analysis

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Performance of CMS muon reconstruction in pp collision events at sqrt(s) = 7 TeV

The performance of muon reconstruction, identification, and triggering in CMS has been studied using 40 inverse picobarns of data collected in pp collisions at sqrt(s) = 7 TeV at the LHC in 2010. A few benchmark sets of selection criteria covering a wide range of physics analysis needs have been examined. For all considered selections, the efficiency to reconstruct and identify a muon with a transverse momentum pT larger than a few GeV is above 95% over the whole region of pseudorapidity covered by the CMS muon system, abs(eta) < 2.4, while the probability to misidentify a hadron as a muon is well below 1%. The efficiency to trigger on single muons with pT above a few GeV is higher than 90% over the full eta range, and typically substantially better. The overall momentum scale is measured to a precision of 0.2% with muons from Z decays. The transverse momentum resolution varies from 1% to 6% depending on pseudorapidity for muons with pT below 100 GeV and, using cosmic rays, it is shown to be better than 10% in the central region up to pT = 1 TeV. Observed distributions of all quantities are well reproduced by the Monte Carlo simulation.Comment: Replaced with published version. Added journal reference and DO