188 research outputs found
Influence of initial pH of the growing medium on the activity, production and genes expression profiles of laccase of Pleurotus ostreatus in submerged fermentations
Background: Enzymatic activity and laccase isoenzymes number of
Pleurotus ostreatus grown in different pH values of the growing
medium in submerged fermentation and incubated in buffer solutions of
different initial pH values were determined. The expression profiles of
five laccase genes (Lacc1, Lacc4, Lacc6, Lacc9 and Lacc10) in these
cultures were also studied. Results: The highest laccases activity was
obtained in cultures grown at initial pH of 4.5 and the lowest in
cultures grown at initial pH of 8.5. Isoenzyme profiles were different
in all the cases. Lacc1, Lacc4, Lacc6 and Lacc10 were expressed in all
the cultures. Conclusions: The initial pH of the growing medium is an
important factor for regulating the expression of laccase genes, having
an effect on the activity and on the laccase isoenzymes number produced
by P. ostreatus in SmF. This is the first report on the influence of
different initial pH values of the growing medium on the laccases
activity, laccase isoenzymes number and laccases expression profiles of
P. ostreatus grown in submerged fermentation
Plant height and hydraulic vulnerability to drought and cold
Understanding how plants survive drought and cold is increasingly important as plants worldwide experience dieback with drought in moist places and grow taller with warming in cold ones. Crucial in plant climate adaptation are the diameters of water-transporting conduits. Sampling 537 species across climate zones dominated by angiosperms, we find that plant size is unambiguously the main driver of conduit diameter variation. And because taller plants have wider conduits, and wider conduits within species are more vulnerable to conduction-blocking embolisms, taller conspecifics should be more vulnerable than shorter ones, a prediction we confirm with a plantation experiment. As a result, maximum plant size should be short under drought and cold, which cause embolism, or increase if these pressures relax. That conduit diameter and embolism vulnerability are inseparably related to plant size helps explain why factors that interact with conduit diameter, such as drought or warming, are altering plant heights worldwide
Total RNA quality of lyophilized and cryopreserved dormant grapevine buds
Background: Plant tissues must be preserved in their collection state,
especially for genome-wide expression profile studies. Lyophilization
is a feasible, affordable tool when fresh tissues cannot be shipped at
ultralow temperatures from their origin to the place of analysis. In
this study, the total RNA quality of dormant grapevine buds ( Vitis
vinifera L. cv. \u2018Flame Seedless\u2019) of freeze-dried
samples stored at room temperature conditions was evaluated and
compared to that of cryopreserved (-80\ub0C) grapevine buds. Results:
Good yield and quality of RNA were obtained from freeze-dried dormant
buds stored at room temperature for 0, 3 and 6 weeks after they were
lyophilized. Further experiments confirmed that the extracted total RNA
could be used for actin and \u3b2-tubulin PCR gene amplification.
Conclusion: High-quality RNA that is useful for downstream applications
was obtained from freeze-dried dormant grapevine bud tissue, similarly
to the RNA obtained from cryopreserved dormant grapevine buds
First Measurement of the W Boson Mass in Run II of the Tevatron
We present a measurement of the W boson mass using 200/pb of data collected
in pbar p collisions at sqrt(s) = 1.96 TeV by the CDF II detector at Run II of
the Fermilab Tevatron. With a sample of 63964 W -> e nu candidates and 51128 W
-> mu nu candidates, we measure M_W = (80413 +- 34 (stat) +- 34 (syst) = 80413
+- 48) MeV/c^2. This is the most precise single measurement of the W boson mass
to date.Comment: published version in PR
Search for New Particles Leading to Z+jets Final States in Collisions at TeV
We present the results of a search for new particles that lead to a \Z boson
plus jets in collisions at TeV using the Collider
Detector at Fermilab (CDF II). A data sample with a luminosity of 1.06 \ifb\
collected using \Z boson decays to and is used. We describe a
completely data-based method to predict the dominant background from
standard-model \Z+jet events. This method can be similarly applied to other
analyses requiring background predictions in multi-jet environments, as shown
when validating the method by predicting the background from +jets in \ttbar
production. No significant excess above the background prediction is observed,
and a limit is set using a fourth generation quark model to quantify the
acceptance. Assuming and using a leading-order
calculation of the cross section, quark masses below 268 \gev/c^2
are excluded at 95% confidence level.Comment: To be submitted to PR
Observation and Mass Measurement of the Baryon
We report the observation and measurement of the mass of the bottom, strange
baryon through the decay chain , where
, , and .
Evidence for observation is based on a signal whose probability of arising from
the estimated background is 6.6 x 10^{-15}, or 7.7 Gaussian standard
deviations. The mass is measured to be (stat.) (syst.) MeV/.Comment: Minor text changes for the second version. Accepted by Phys. Rev.
Let
Polarizations of J/psi and psi(2S) Mesons Produced in ppbar Collisions at 1.96 TeV
We have measured the polarizations of \jpsi and \psiprime mesons as
functions of their transverse momentum \pt when they are produced promptly in
the rapidity range with \pt \geq 5 \pgev. The analysis is performed
using a data sample with an integrated luminosity of about 800 \ipb collected
by the CDF II detector. For both vector mesons, we find that the polarizations
become increasingly longitudinal as \pt increases from 5 to 30 \pgev. These
results are compared to the predictions of nonrelativistic quantum
chromodynamics and other contemporary models. The effective polarizations of
\jpsi and \psiprime mesons from -hadron decays are also reported.Comment: 8 pages, 7 figures, published in Physical Review Letter
Precise measurement of the top quark mass in the lepton+jets topology at CDF II
We present a measurement of the mass of the top quark from proton-antiproton
collisions recorded at the CDF experiment in Run II of the Fermilab Tevatron.
We analyze events from the single lepton plus jets final state (). The top quark mass is extracted
using a direct calculation of the probability density that each event
corresponds to the final state. The probability is a function of both
the mass of the top quark and the energy scale of the calorimeter jets, which
is constrained {\it in situ} by the hadronic boson mass. Using 167 events
observed in 955 pb of integrated luminosity, we achieve the single
most precise measurement of the top quark mass, 170.8 2.2 (stat.)
1.4 (syst.) GeV/.Comment: accepted by Phys. Rev. Let
Search for resonant ttbar production in ppbar collisions at sqrt{s}=1.96 TeV
We report on a search for narrow-width particles decaying to a top and
antitop quark pair. The data set used in the analysis corresponds to an
integrated luminosity of 680 pb^{-1} collected with the Collider Detector at
Fermilab in Run II. We present 95% confidence level upper limits on the cross
section times branching ratio. Assuming a specific topcolor-assisted
technicolor production model, the leptophobic Z' with width
\Gamma_{Z'}=0.012M_{Z'}, we exclude the mass range M_{Z'} < 725 GeV/c^2 at the
95% confidence level.Comment: 7 pages, 3 figures. submitted to Phys. Rev. Let
Strange particle production in proton-proton collisions at TeV with ALICE at the LHC
The production of mesons containing strange quarks (K, ) and both
singly and doubly strange baryons (, Anti-, and
+Anti-) are measured at central rapidity in pp collisions at
= 0.9 TeV with the ALICE experiment at the LHC. The results are
obtained from the analysis of about 250 k minimum bias events recorded in 2009.
Measurements of yields (dN/dy) and transverse momentum spectra at central
rapidities for inelastic pp collisions are presented. For mesons, we report
yields () of 0.184 0.002 stat. 0.006 syst. for K and
0.021 0.004 stat. 0.003 syst. for . For baryons, we find
= 0.048 0.001 stat. 0.004 syst. for , 0.047
0.002 stat. 0.005 syst. for Anti- and 0.0101 0.0020 stat.
0.0009 syst. for +Anti-. The results are also compared with
predictions for identified particle spectra from QCD-inspired models and
provide a baseline for comparisons with both future pp measurements at higher
energies and heavy-ion collisions.Comment: 33 pages, 21 captioned figures, 10 tables, authors from page 28,
published version, figures at
http://aliceinfo.cern.ch/ArtSubmission/node/387
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