Multicolor Photometry of the Uranus Irregular Satellites Sycorax and Caliban

We report on accurate BVRI photometry for the two Uranus irregular satellites Sycorax and Caliban. We derive colours, showing that Sycorax is bluer than Caliban. Our data allows us to detect a significant variability in the Caliban's light-curve, which suggests an estimated period of about 3 hours. Despite it is the brighter of the two bodies, Sycorax does not display a strong statistically significant variability. However our data seem to suggest a period of about 4 hoursComment: 17 pages, 2 eps figures, in press in Astronomical Journa

Evidence for the formation of comet 67P/Churyumov-Gerasimenko through gravitational collapse of a bound clump of pebbles

The processes that led to the formation of the planetary bodies in the Solar System are still not fully understood. Using the results obtained with the comprehensive suite of instruments on-board ESA’s Rosetta mission, we present evidence that comet 67P/Churyumov-Gerasimenko likely formed through the gentle gravitational collapse of a bound clump of mm-sized dust aggregates (“pebbles”), intermixed with microscopic ice particles. This formation scenario leads to a cometary make-up that is simultaneously compatible with the global porosity, homogeneity, tensile strength, thermal inertia, vertical temperature profiles, sizes and porosities of emitted dust, and the steep increase in water-vapour production rate with decreasing heliocentric distance, measured by the instruments on-board the Rosetta spacecraft and the Philae lander. Our findings suggest that the pebbles observed to be abundant in protoplanetary discs around young stars provide the building material for comets and other minor bodies

Putative histidine kinase inhibitors with antibacterial effect against multi-drug resistant clinical isolates identified by in vitro and in silico screens

Novel antibacterials are urgently needed to address the growing problem of bacterial resistance to conventional antibiotics. Two-component systems (TCS) are widely used by bacteria to regulate gene expression in response to various environmental stimuli and physiological stress and have been previously proposed as promising antibacterial targets. TCS consist of a sensor histidine kinase (HK) and an effector response regulator. The HK component contains a highly conserved ATP-binding site that is considered to be a promising target for broad-spectrum antibacterial drugs. Here, we describe the identification of putative HK autophosphorylation inhibitors following two independent experimental approaches: in vitro fragment-based screen via differential scanning fluorimetry and in silico structure-based screening, each followed up by the exploration of analogue compounds as identified by ligand-based similarity searches. Nine of the tested compounds showed antibacterial effect against multi-drug resistant clinical isolates of bacterial pathogens and include three novel scaffolds, which have not been explored so far in other antibacterial compounds. Overall, putative HK autophosphorylation inhibitors were found that together provide a promising starting point for further optimization as antibacterials

How Comets Work

Two major questions regarding comets have been up to now far from any solution. (i) How is it possible that water-ice sublimation from the nucleus surface does not lead to an insulating crust, stopping every gas and dust ejection within a few days? (ii) How is it possible that the gas flow crossing the refractory surface crust ejects dust particles bonded by tensile strengths larger than tens of Pa when the perihelion gas pressure at the nucleus-coma interface is less than one Pa? We have developed a simple but rigorous analytical model, assuming that the cometary nucleus consists of agglomerates of ice and dust (“clusters”). As soon as the clusters become exposed to sunlight, gas diffusion from their inside leads to their dehydration. We find that (i) the gas diffusing from the interior to the surface of a sunlit cluster has a steep density gradient at the cluster surface, which blasts the cluster into particles of sizes larger than or equal to those actually observed by Rosetta dust instruments; (ii) the heat-conduction and diffusion timescales are much shorter than the dehydration timescale, ensuring that the described process prevents any dumping of the nucleus activity driven by water-ice sublimation from 4 au inbound to 4 au outbound; and (iii) the clusters are in fact cm-sized pebbles, so that a cometary nucleus made of pebbles is confirmed to be the only one consistent with cometary gas and dust activity, a process unexplained until now

67P/C-G inner coma dust properties from 2.2 au inbound to 2.0 auoutbound to the Sun

GIADA (Grain Impact Analyzer and Dust Accumulator) on-board the Rosetta space probe is designed to measure the momentum, mass and speed of individual dust particles escaping the nucleus of comet 67P/Churyumov-Gerasimenko (hereafter 67P). From 2014 August to 2016 June, Rosetta escorted comet 67P during its journey around the Sun. Here, we focus on GIADA data taken between 2015 January and 2016 February which included 67P's perihelion passage. To better understand cometary activity and more specifically the presence of dust structures in cometary comae, we mapped the spatial distribution of dust density in 67P's coma. In this manner, we could track the evolution of high-density regions of coma dust and their connections with nucleus illumination conditions, namely tracking 67P's seasons. We also studied the link between dust particle speeds and their masses with respect to heliocentric distance, i.e. the level of cometary activity. This allowed us to derive a global and a local correlation of the dust particles' speed distribution with respect to the H2O production rate. © 2016 The Authors.Peer Reviewe

Extracellular GTP is a Potent Water- Transport Regulator via Aquaporin 5 Plasma-Membrane Insertion in M1-CCD Epithelial Cortical Collecting Duct Cells

Background/Aims: Extracellular GTP is able to modulate some specific functions in neuron, glia and muscle cell models as it has been demonstrated over the last two decades. In fact, extracellular GTP binds its specific plasma membrane binding sites and induces signal transduction via [Ca(2+)]i increase. We demonstrate, for the first time, that extracellular GTP is able to modulate cell swelling in M1-CCD cortical collecting duct epithelial cells via upregulation of aquaporin 5 (AQP5) expression. Methods: We used videoimaging, immunocitochemistry, flow cytometry, confocal techniques, Western blotting and RT-PCR for protein and gene expression analysis, respectively. Results: We demonstrate that AQP5 mRNA is up-regulated 7 h after the GTP exposure in the cell culture medium, and its protein level is increased after 12-24 h. We show that AQP5 is targeted to the plasma membrane of M1-CCD cells, where it facilitates cell swelling, and that the GTP-dependent AQP5 up-regulation occurs via [Ca(2+)]i increase. Indeed, GTP induces both oscillating and transient [Ca(2+)]i increase, and specifically the oscillating kinetic appears to be responsible for blocking cell cycle in the S-phase while the [Ca(2+)]i influx, with whatever kinetic, seems to be responsible for inducing AQP5 expression. Conclusion: The role of GTP as a regulator of AQP5-mediated water transport in renal cells is of great importance in the physiology of renal epithelia, due to its possible physiopathological implications. GTP-dependent AQP5 expression could act as osmosensor. In addition, the data presented here suggest that GTP might play the same role in other tissues where rapid water transport is required for cell volume regulation and maintenance of the homeostasis. © 2014 S. Karger AG, Basel. ispartof: Cellular Physiology and Biochemistry vol:33 issue:3 pages:731-46 ispartof: location:Germany status: publishe

Dynamics of non-spherical dust in the coma of 67P/Churyumov- Gerasimenko constrained by GIADA and ROSINA data

Among the comet 67P/Churyumov-Gerasimenko (67P/C-G) in situ measurements, the closest that have ever been performed at a comet nucleus, are also those of speed, mass, and cross-section of cometary grains performed by the Grain Impact Analyser and Dust Accumulator (GIADA) instrument. To interpret GIADA data, we performed dust dynamical numerical simulations with both spherical and non-spherical (spheroids) shapes. This allowed us to analyse how the grain non-sphericity affects the data interpretation. We find that some measured dust speeds are unlikely reproducible when a spherical shape is considered. We considered two GIADA observational periods, 2015 February 19-27 and 2015 March 13-28. Gas parameters calibrated with the Rosetta Orbiter Spectrometer for Ion and Neutral Analysis (ROSINA) measurements have been used to retrieve the gas conditions to set up the dust particle motion. The dust grains are assumed to be out of the near nucleus coma, i.e. where the gas velocity is radial and constant, therefore they are either aligned or have random but constant orientation with respect to the gas drag. We reproduced the GIADA dust speeds, using spheres and two different spheroidal shapes. We find that the particle shapes that reproduce best the GIADA dust speeds are consistent with the particle shape constrained by the GIADA data. We obtain different terminal velocities for spherical and non-spherical particles of the same mass. The shape, which reproduces the GIADA data, is oblate rather than prolate spheroid. We obtain rotational frequencies of the spheroidal particles that best fit the GIADA measurements in these periods

Endurance-dependent urinary extracellular vesicle signature: shape, metabolic miRNAs, and purine content distinguish triathletes from inactive people

Extracellular vesicles (EVs) enriched with bioactive molecules have gained considerable attention in nanotechnology because they are critical to intercellular communication while maintaining low immunological impact. Among biological matrices, urine has emerged as a noninvasive source of extracellular-contained liquid biopsy, currently of interest as a readout for physiological adaptations. Therefore, we aimed to evaluate chronic adaptations of endurance sport practice in terms of urinary EV parameters and evaluated by food consumption assessment. Two balanced groups of 13 inactive controls vs. triathlon athletes were enrolled; their urinary EVs were obtained by differential ultracentrifugation and analyzed by dynamic light scattering and transmission electron and atomic force microscopy. The cargo was analyzed by means of purine and miRNA content through HPLC-UV and qRT-PCR. Specific urinary EV signatures differentiated inactive versus endurance-trained in terms of peculiar shape. Particularly, a spheroid shape, smaller size, and lower roughness characterize EVs from triathletes. Metabolic and regulatory miRNAs often associated with skeletal muscle (i.e., miR378a-5p, miR27a-3p, miR133a, and miR206) also accounted for a differential signature. These miRNAs and guanosine in urinary EVs can be used as a readout for metabolic status along with the shape and roughness of EVs, novel informative parameters that are rarely considered. The network models allow scholars to entangle nutritional and exercise factors related to EVs' miRNA and purine content to depict metabolic signatures. All in all, multiplex biophysical and molecular analyses of urinary EVs may serve as promising prospects for research in exercise physiology