28 research outputs found
Variación antigénica de la cepa Munantá de Trypanosoma cruzí después de pase por ratón
This study evaluated the isoenzyme and antigenic changes of Trypanosoma cruzfs Munanta strain after two consecutive passages in mice, using isoenzyme electrophoresis, SDS-PAGE electrophoresis and immunoblot. The results obtained show significant statistical differences within the strain before and after animal passages, which suggests that the mouse is not a recommended model for obtaining the parasite's Munanta strain's trypomastigote forms destined for study of the immune response.Este estudio evaluó los cambios en el perfil isoenzimático y antigénico de la cepa Munantá de Trypanosoma cruzidespués de dos pases consecutivos por ratón, mediante electrofóresis isoenzimática, electrofóresis en PAGE-SDS e immunoblot. Los resultados obtenidos muestran diferencias estadísticamente significativas entre la cepa antes y después de los pases en los animales, lo cual sugiere que el ratón no es un modelo recomendable para obtener las formas tripomastigotas de la cepa Munanta del parásito destinadas a estudios de la respuesta inmune
Erratum to: Scaling up strategies of the chronic respiratory disease programme of the European Innovation Partnership on Active and Healthy Ageing (Action Plan B3: Area 5).
[This corrects the article DOI: 10.1186/s13601-016-0116-9.]
Erratum to: Scaling up strategies of the chronic respiratory disease programme of the European Innovation Partnership on Active and Healthy Ageing (Action Plan B3: Area 5).
[This corrects the article DOI: 10.1186/s13601-016-0116-9.]
Erratum to: Scaling up strategies of the chronic respiratory disease programme of the European Innovation Partnership on Active and Healthy Ageing (Action Plan B3: Area 5)
Scaling up strategies of the chronic respiratory disease programme of the European Innovation Partnership on Active and Healthy Ageing (Action Plan B3: Area 5)
Abstract Action Plan B3 of the European Innovation Partnership on Active and Healthy Ageing (EIP on AHA) focuses on the integrated care of chronic diseases. Area 5 (Care Pathways) was initiated using chronic respiratory diseases as a model. The chronic respiratory disease action plan includes (1) AIRWAYS integrated care pathways (ICPs), (2) the joint initiative between the Reference site MACVIA-LR (Contre les MAladies Chroniques pour un VIeillissement Actif) and ARIA (Allergic Rhinitis and its Impact on Asthma), (3) Commitments for Action to the European Innovation Partnership on Active and Healthy Ageing and the AIRWAYS ICPs network. It is deployed in collaboration with the World Health Organization Global Alliance against Chronic Respiratory Diseases (GARD). The European Innovation Partnership on Active and Healthy Ageing has proposed a 5-step framework for developing an individual scaling up strategy: (1) what to scale up: (1-a) databases of good practices, (1-b) assessment of viability of the scaling up of good practices, (1-c) classification of good practices for local replication and (2) how to scale up: (2-a) facilitating partnerships for scaling up, (2-b) implementation of key success factors and lessons learnt, including emerging technologies for individualised and predictive medicine. This strategy has already been applied to the chronic respiratory disease action plan of the European Innovation Partnership on Active and Healthy Ageing
Trypanosoma cruzi I genotype among isolates from patients with chronic Chagas disease followed at the Evandro Chagas National Institute of Infectious Diseases (FIOCRUZ, Brazil)
The participative management for the quality of the performance of the teachers. A study of case
El presente trabajo tuvo como propósito analizar la Gerencia
Participativa para la Calidad del Desempeño de los docentes de la
III Etapa de Educación Básica, para lo cual se tomo como caso de
estudio la Unidad Educativa «María Bartola Becerra»; ubicada
en el Municipio y Estado Trujillo. La Gerencia Participativa se
fundamenta en las Teorías Humanista-Conductista,
Socio-psicológica y Sistémica. La metodología se enmarcó dentro
de la investigación cualitativa (participativa) – cuantitativa, de tipo
descriptiva – explicativa, con un diseño de campo. La población
estuvo conformada por dos subpoblaciones: 3 directivos y 18
docentes, de estos últimos se extrajo una muestra representativa
del 30%. Como técnicas se emplearon la observación participante
y la entrevista, como instrumentos la entrevista semi-estructurada,
abierta y el cuestionario; con validación de contenido mediante
juicio de expertos. Se incorporaron 5 expertos como informantes
clave. El análisis de los resultados se llevó a cabo por medio de la
categorización, codificación y análisis de contenido, mediante la
complementación y triangulación. Las conclusiones develaron como
aspectos favorables en los docentes la formación profesional,
actualización, valores morales – éticos, función de mediador y
orientador, planificación, innovación y satisfacción. Las principales
debilidades se orientaron hacia el ejercicio del poder y autoridad,
delegación de funciones, trabajo en equipo, relaciones laborales y
proceso comunicacional, todas ellas con posibilidades de superarse
mediante la puesta en práctica de la gerencia participativa.
Finalmente se elaboró una propuesta para formar y educar a
directivos y docentes bajo los lineamientos de la Gerencia
Participativa buscando un desempeño docente de [email protected]@[email protected] purpose of this study was to analyze the participatory
management for the quality of teacher perfomance in the third stage
of basic education, taking as a case study of the educational unit
of the minicipality Becerra María Bartola. Trujillo. Participative
management theories is based on Humanity, behavioral,
sociopsychological and systemic. The methodology was framed
within the qualitative and quantitative rescarch is a descriptiveexplanatory,
with a field design. The population was componed of
three directors and 18 teachers in the simple was taken as 30%. As
techniques were used particpant observation and interviews. As
instruments, the questionnaire The methodology was framed within
the qualitative and quantitative reserche is a descriptive-explanatory,
with a field design. The population was componed of three directors
and 18 teacher in the sample was taken as 30%. As techniques
were used particpant observation and interviews. As instruments,
the questionnaire The findings unveiled as strengths in training
teachers, updating, moral and ethical values, role of mediator and
counselor, planning, innovation and satisfaction. The main
weaknesses identified were oriented towards the exercise of power
and authority, delegation of tasks The findings unveiled as strengths
in training teacher, updating, moral and ethical values, role of
mediator and conunselor, planning, innovation and satisfaction.
The main weaknesses identified were oriented towards the exercise
of power and authority, delegation of tasks teamwork, working
relationships and communication process. All of them, with
opportunities to excel through the practice of participative
management. Finally, a proposal was made to train and educate
principals and teachers Ander the guidance of the Participatory
Management looking for a quality teacher performance
Enfermedad de Chagas aguda en Colombia, una entidad poco sospechada. Informe de 10 casos presentados en el periodo 2002 a 2005
Introducción. Los casos de enfermedad de Chagas aguda informados en Colombia son esporádicos.
Objetivo. Describir 10 casos notificados al Grupo de Parasitología del Instituto Nacional de Salud entre 2002 y 2005.
Materiales y métodos. Utilizando información de historias clínicas, fichas epidemiológicas, informes de resultados de exámenes de laboratorio y análisis de sangre, se tabularon variables demográficas, hallazgos clínicos, paraclínicos y de laboratorio. Se realizaron extendidos de sangre periférica, identificación serológica para IgG mediante inmunofluorescencia indirecta, aislamiento en cultivo e inoculación en ratones, pruebas de reacción en cadena de la polimerasa y análisis isoenzimático.
Resultados. El 100% de los casos procedían de zonas endémicas; tres se informaron en Putumayo, dos en cada uno de los departamentos de Arauca, Casanare, Norte de Santander y uno en Santander. La presunta vía de transmisión fue vectorial; en el 30% había convivencia con el vector. El 70% fueron adultos entre los 18 y los 50 años y el 30% niños entre los seis meses y los dos años. El síntoma predominante fue fiebre en el 90%. Los signos de puerta de entrada fueron infrecuentes; solamente un paciente presentó un probable signo de Romaña. Tres pacientes presentaron miocarditis, dos desarrollaron falla cardiaca, y uno taponamiento cardíaco. La parasitemia fue evidente en nueve casos; las pruebas serológicas fueron reactivas en cinco casos y también en cinco casos se logró aislamiento del parásito. El análisis isoenzimático identificó Trypanosoma cruzi grupo I.
Conclusiones. La variabilidad clínica predominó. Ningún caso se sospechó clínicamente. Es importante incluir esta enfermedad como diagnóstico diferencial del síndrome febril en regiones endémicas debido a su buena respuesta al tratamiento etiológico el cual previene la fase crónica
Dispersion of oil into water using lecithin-Tween 80 blends: The role of spontaneous emulsification
Impact of castration with or without alpha-tocopherol supplementation on the urethral sphincter of rats
OBJECTIVE: To analyze the impact of low levels of testosterone induced by orchiectomy and the effect of alpha-tocopherol supplementation on oxidative stress in the urethral sphincter. MATERIALS AND METHODS: Forty male Wistar rats weighing 250-300g were divided into four groups with 10 each: Sham group; Orchiectomy group: bilateral orchiectomy; Orchiectomy-pre-Tocopherol group: bilateral orchiectomy preceded by alpha-tocopherol supplementation for four weeks; Orchiectomy-full-Tocopherol group: bilateral orchiectomy with alpha-tocopherol supplementation for four weeks preceding the procedure and for eight weeks afterwards. At the protocol end, animals were euthanized and had the sphincter analyzed stereologically focusing on collagen and muscle fibers percentage. Oxidative stress levels were determined using 8-epi-PGF2. RESULTS: The 8-epi-PGF2 levels were statistically higher (p < 0.0003) in the Orchiectomy group compared to others groups while Sham and Orchiectomy-full-Tocopherol groups presented statistically similar values (p = 0.52). Collagen volumetric densities were significantly lower in Sham and Orchiectomy-full-Tocopherol groups (p < 0.022). Sham group presented statistically greater muscle fiber percent. CONCLUSION: Castration caused oxidative stress in the urethral sphincter complex, with increased collagen deposition. Alpha-tocopherol had a protective effect and its supplementation for twelve weeks provided the greatest protection