27 research outputs found
PRODU\uc7\uc3O DE BIOMASSA E CONTE aDO DE FEN 3IS E FLAVONOIDES DE Schinus terebinthifolius CULTIVADA EM FILEIRA SIMPLES E DUPLA COM CAMA DE FRANGO
The aim of this study was to evaluate the influence of the addition of
poultry litter on growth, biomass yield, flavonoid and phenol content,
and antioxidant activity in leaves of pink pepper. The experiment was
carried out at the Federal University of Grande Dourados, in Dourados,
Mato Grosso do Sul state, from October 2009 to May 2010. Pink pepper
was grown in single or double row in soil with incorporated poultry
litter at doses of 0, 5, 10, 15 and 20 t ha-1. Treatments were arranged
as 2 x 5 factorial in a randomized block design with four replications.
There was a significant interaction between the doses of poultry litter
and evaluation times for plant height and chlorophyll content. A linear
increase in leaf area, fresh and dry weight of leaves and diameter of
main stems with increasing doses of poultry litter was observed. Plants
grown in single row showed higher fresh weight of stem with increasing
doses of poultry litter. Poultry litter at 15 and 20 t ha-1 promoted an
increase in flavonoid and phenolic contents in the leaves. No
significant effect on the antioxidant activity was observed by the
chemical method using DPPH. Therefore, it is recommended the
cultivation of pink pepper plants with double row and 20 t ha-1 of
poultry litter to higher growth, biomass yield, and flavonoid and
phenol content.O objetivo deste estudo foi avaliar a influ\ueancia da
adi\ue7\ue3o da cama de frango ao solo sobre o
crescimento,produ\ue7\ue3o de biomassa, conte\ufado de
fen\uf3is e flavonoides e atividade antioxidante em folhas de
pimentarosa. O experimento foi realizado na Universidade Federal da
Grande Dourados, em Dourados-MS, de outubro de 2009 a maio de 2010. A
pimenta-rosa foi cultivada em fileira simples e dupla com cama de
frango incorporada nas doses de 0, 5, 10, 15 e 20 t ha-1. Os
tratamentos foram arranjados em fatorial 2 x 5 em blocos casualizados
com quatro repeti\ue7\uf5es. Houve uma intera\ue7\ue3o
significativa entre doses de cama de frango e \ue9pocas de
avalia\ue7\ue3o para altura de plantas e conte\ufado de
clorofila. Foi observado um aumento linear na \ue1rea foliar, massa
fresca e seca de folhas e di\ue2metro dos ramos principais com o
aumento das doses de cama de frango. As plantas cultivadas em fileira
simples apresentaram maior massa fresca de caules com o aumento das
doses de cama de frango. A cama de frango nas doses de 15 e 20 t ha-1
promoveu um incremento no conte\ufado de fen\uf3is e flavonoides
nas folhas. Nenhum efeito significativo na atividade antioxidante foi
observado com o m\ue9todo qu\uedmico utilizando DPPH. Portanto,
recomenda-se o cultivo de plantas de pimenta-rosa em fileira dupla e 20
t ha-1 de cama de frango para maior crescimento, produ\ue7\ue3o de
biomassa, e conte\ufado de fen\uf3is e flavonoides
Identification of target genes for wild type and truncated HMGA2 in mesenchymal stem-like cells
Background
The HMGA2 gene, coding for an architectural transcription factor involved in mesenchymal embryogenesis, is frequently deranged by translocation and/or amplification in mesenchymal tumours, generally leading to over-expression of shortened transcripts and a truncated protein.
Methods
To identify pathways that are affected by sarcoma-associated variants of HMGA2, we have over-expressed wild type and truncated HMGA2 protein in an immortalized mesenchymal stem-like cell (MSC) line, and investigated the localisation of these proteins and their effects on differentiation and gene expression patterns.
Results
Over-expression of both transgenes blocked adipogenic differentiation of these cells, and microarray analysis revealed clear changes in gene expression patterns, more pronounced for the truncated protein. Most of the genes that showed altered expression in the HMGA2-overexpressing cells fell into the group of NF-ÎșB-target genes, suggesting a central role for HMGA2 in this pathway. Of particular interest was the pronounced up-regulation of SSX1, already implicated in mesenchymal oncogenesis and stem cell functions, only in cells expressing the truncated protein. Furthermore, over-expression of both HMGA2 forms was associated with a strong repression of the epithelial marker CD24, consistent with the reported low level of CD24 in cancer stem cells.
Conclusions
We conclude that the c-terminal part of HMGA2 has important functions at least in mesenchymal cells, and the changes in gene expression resulting from overexpressing a protein lacking this domain may add to the malignant potential of sarcomas
All-sky search for gravitational-wave bursts in the second joint LIGO-Virgo run
We present results from a search for gravitational-wave bursts in the data
collected by the LIGO and Virgo detectors between July 7, 2009 and October 20,
2010: data are analyzed when at least two of the three LIGO-Virgo detectors are
in coincident operation, with a total observation time of 207 days. The
analysis searches for transients of duration < 1 s over the frequency band
64-5000 Hz, without other assumptions on the signal waveform, polarization,
direction or occurrence time. All identified events are consistent with the
expected accidental background. We set frequentist upper limits on the rate of
gravitational-wave bursts by combining this search with the previous LIGO-Virgo
search on the data collected between November 2005 and October 2007. The upper
limit on the rate of strong gravitational-wave bursts at the Earth is 1.3
events per year at 90% confidence. We also present upper limits on source rate
density per year and Mpc^3 for sample populations of standard-candle sources.
As in the previous joint run, typical sensitivities of the search in terms of
the root-sum-squared strain amplitude for these waveforms lie in the range 5
10^-22 Hz^-1/2 to 1 10^-20 Hz^-1/2. The combination of the two joint runs
entails the most sensitive all-sky search for generic gravitational-wave bursts
and synthesizes the results achieved by the initial generation of
interferometric detectors.Comment: 15 pages, 7 figures: data for plots and archived public version at
https://dcc.ligo.org/cgi-bin/DocDB/ShowDocument?docid=70814&version=19, see
also the public announcement at
http://www.ligo.org/science/Publication-S6BurstAllSky
Genomic analyses identify hundreds of variants associated with age at menarche and support a role for puberty timing in cancer risk
The timing of puberty is a highly polygenic childhood trait that is epidemiologically associated with various adult diseases. Using 1000 Genomes Projectâimputed genotype data in up to ~370,000 women, we identify 389 independent signals (P < 5 Ă 10) for age at menarche, a milestone in female pubertal development. In Icelandic data, these signals explain ~7.4% of the population variance in age at menarche, corresponding to ~25% of the estimated heritability. We implicate ~250 genes via coding variation or associated expression, demonstrating significant enrichment in neural tissues. Rare variants near the imprinted genes MKRN3 and DLK1 were identified, exhibiting large effects when paternally inherited. Mendelian randomization analyses suggest causal inverse associations, independent of body mass index (BMI), between puberty timing and risks for breast and endometrial cancers in women and prostate cancer in men. In aggregate, our findings highlight the complexity of the genetic regulation of puberty timing and support causal links with cancer susceptibility
Morphological and photosynthetic adaptations of Tabebuia aurea seedlings in the nursery
Tabebuia aurea (Benth. & Hook. f. ex S. Moore) (Bignoniaceae) is a boreal species common in Brazil. It is used for ornamental parks and along sidewalks. Its timber is also used for furniture. The objective of this study was to evaluate the effect of nursery shading on the growth and photosynthesis of T. aurea and their photosynthetic adaptation after being transferred to direct sunlight. The T. aurea seedlings were grown under 0, 50, 70 or 95% shade. The photosynthetic active radiation and leaf gas exchange were measured over two distinct periods: 51 (young seedlings) and 70 days after having been sown under each shade treatment. Immediately after the measurements were taken, the seedlings were transferred into full sunlight and the measurements were repeated two times after 15 min and 3 days under ambient sunlight. T. aurea seedlings showed satisfactory growth up to 50% shade in the nursery, which could be verified both by growth measurement and by total biomass accumulation. Shading greater than 70% reduced the number of leaves, the leaf area and the stem diameter in relation to plants exposed to full sunlight. The results suggest that T. aurea seedlings should be grown under full sunlight or under shading up to 50% to maximize their growth in the nursery and to minimize stress when transferring the seedlings to their final planting sites
Frequency of pleural effusion in dengue patients by severity, age and imaging modality: a systematic review and meta-analysis
Abstract Background Identification of pleural effusion (PE) in dengue infection is an objective measure of plasma leakage and may predict disease progression. However, no studies have systematically assessed the frequency of PE in patients with dengue, and whether this differs across age and imaging modality. Methods We searched Pubmed, Embase Web of Science and Lilacs (period 1900â2021) for studies reporting on PE in dengue patients (hospitalized and outpatient). We defined PE as fluid in the thoracic cavity detected by any imaging test. The study was registered in PROSPERO (CRD42021228862). Complicated dengue was defined as hemorrhagic fever, dengue shock syndrome or severe dengue. Results The search identified 2,157 studies of which 85 studies were eligible for inclusion. The studies (nâ=â31 children, nâ=â10 adults, nâ=â44 mixed age) involved 12,800 patients (30% complicated dengue). The overall frequency of PE was 33% [95%CI: 29 to 37%] and the rate of PE increased significantly with disease severity (Pâ=â0.001) such that in complicated vs. uncomplicated dengue the frequencies were 48% and 17% (Pâ<â0.001). When assessing all studies, PE occurred significantly more often in children compared to adults (43% vs. 13%, Pâ=â0.002) and lung ultrasound more frequently detected PE than conventional chest X-ray (Pâ=â0.023). Conclusions We found that 1/3 of dengue patients presented with PE and the frequency increased with severity and younger age. Importantly, lung ultrasound demonstrated the highest rate of detection. Our findings suggest that PE is a relatively common finding in dengue and that bedside imaging tools, such as lung ultrasound, potentially may enhance detection