Impact of membrane pore structure on protein detection sensitivity of affi nity-based immunoassay

Understanding a membrane’s morphology is important for controlling its fi nal performance during protein immobilization. Porous, symmetric membranes were prepared from a polyvinylidene fl uoride/N-methyl-2-pyrrolidinone solution by phase inversion process, to obtain membrane with various microsized pores. The concentration and surface area of aprotein dotted on the membrane surface were measured by staining with Ponceau S dye. The dotted protein was further scanned and analysed to perform quantitative measurements for relative comparison. The intensity of the red protein spot and its surface area varied depending on the membrane pore size, demonstrating the dependence of protein immobilization on this factor. The membrane with the smallest pore size (M3) showed the highest protein spot intensity and surface area when examined at different protein concentrations. An increase in the applied protein volume showed a linearity proportional trend to the total surface area, and an uneven round dot shape was observed at a large applied volume of protein solution

Diffusion in supersonic, turbulent, compressible flows

We investigate diffusion in supersonic, turbulent, compressible flows. Supersonic turbulence can be characterized as network of interacting shocks. We consider flows with different rms Mach numbers and where energy necessary to maintain dynamical equilibrium is inserted at different spatial scales. We find that turbulent transport exhibits super-diffusive behavior due to induced bulk motions. In a comoving reference frame, however, diffusion behaves normal and can be described by mixing length theory extended into the supersonic regime.Comment: 11 pages, incl. 5 figures, accepted for publication in Physical Review E (a high-resolution version is available at http://www.aip.de./~ralf/Publications/p21.abstract.html

Quantized bulk fermions in the Randall-Sundrum brane model

The lowest order quantum corrections to the effective action arising from quantized massive fermion fields in the Randall-Sundrum background spacetime are computed. The boundary conditions and their relation with gauge invariance are examined in detail. The possibility of Wilson loop symmetry breaking in brane models is also analysed. The self-consistency requirements, previously considered in the case of a quantized bulk scalar field, are extended to include the contribution from massive fermions. It is shown that in this case it is possible to stabilize the radius of the extra dimensions but it is not possible to simultaneously solve the hierarchy problem, unless the brane tensions are dramatically fine tuned, supporting previous claims.Comment: 25 pages, 1 figure, RevTe

Three-Nucleon Force Effects in Nucleon Induced Deuteron Breakup: Comparison to Data (II)

Selected Nd breakup data over a wide energy range are compared to solutions of Faddeev equations based on modern high precision NN interactions alone and adding current three-nucleon force models. Unfortunately currently available data probe phase space regions for the final three nucleon momenta which are rather insensitive to 3NF effects as predicted by current models. Overall there is good to fair agreement between present day theory and experiment but also some cases exist with striking discrepancies. Regions in the phase space are suggested where large 3NF effects can be expected.Comment: 33 pages, 24 ps figures, 9 gif figure

On the Holographic RG-flow and the Low-energy, Strong Coupling, Large N Limit

From the AdS/CFT correspondence, we learn that the classical evolution of supergravity in the bulk can be reduced to a RG-flow equation for the dual low-energy, strongly coupled and large N gauge theory on the boundary. This result has been used to obtain interesting relations between the various terms in the gravitational part of the boundary effective action, in particular the term that affect the cosmological constant. It is found that once the cosmological constant is cancelled in the UV theory, the RG-flow symmetry of the boundary effective action automatically implies the existence of zero cosmological constant solutions that extend all the way into the IR. Given the standard (and well founded) contradiction between the RG-flow idea and the observational evidence of a small cosmological constant, this is considered to be an important progress, albeit incomplete, towards the final solution. Motivated by this success, it would be interesting to see whether this RG-stability extends outside the scope of strong 't Hooft coupling and large N regime that are implicitly assumed in the de Boer-Verlinde-Verlinde Hamilton-Jacobi formulation of the holographic RG-flow equations of the boundary theory. In this paper, we address this question. Taking into account the leading order corrections in the 1/N and $\alpha'/{R^2}$ parameters, we derive new bulk/boundary relations, from which one can read all the local terms in the boundary effective action. Next, we use the resulting constraints, to examine whether the RG-stability of the cosmological constant extends to the new coupling regime. It would be also interesting to use these constraints to study the Randall-Sundrum scenario in this case.Comment: 27 pages, LateX, no figures, minor changes, typos corrected and added more reference

Measurements of differential production cross sections for a Z boson in association with jets in pp collisions at root s=8 TeV

Peer reviewe

Search for lepton flavour violating decays of heavy resonances and quantum black holes to an eμ pair in proton–proton collisions at √s = 8 TeV

A search for narrow resonances decaying to an electron and a muon is presented. The eμ mass spectrum is also investigated for non-resonant contributions from the production of quantum black holes (QBHs). The analysis is performed using data corresponding to an integrated luminosity of 19.7 fb-1 collected in proton-proton collisions at a centre-of-mass energy of 8 TeV with the CMS detector at the LHC. With no evidence for physics beyond the standard model in the invariant mass spectrum of selected eμ pairs, upper limits are set at 95 % confidence level on the product of cross section and branching fraction for signals arising in theories with charged lepton flavour violation. In the search for narrow resonances, the resonant production of τ sneutrino in R-parity violating supersymmetry is considered. The τ sneutrino is excluded for masses below 1.28 TeV for couplings λ132= λ231= λ311′= 0.01 , and below 2.30 TeV for λ132= λ231= 0.07 and λ311′= 0.11. These are the most stringent limits to date from direct searches at high-energy colliders. In addition, the resonance searches are interpreted in terms of a model with heavy partners of the Z boson and the photon. In a framework of TeV-scale quantum gravity based on a renormalization of Newton’s constant, the search for non-resonant contributions to the eμ mass spectrum excludes QBH production below a threshold mass Mth of 1.99 TeV. In models that invoke extra dimensions, the bounds range from 2.36 TeV for one extra dimension to 3.63 TeV for six extra dimensions. This is the first search for QBHs decaying into the eμ final state

Measurement of prompt Jψ\psi pair production in pp collisions at \sqrt s = 7 Tev

Production of prompt J/ ψ meson pairs in proton-proton collisions at s s√ = 7 TeV is measured with the CMS experiment at the LHC in a data sample corresponding to an integrated luminosity of about 4.7 fb −1 . The two J/ ψ mesons are fully reconstructed via their decays into μ + μ − pairs. This observation provides for the first time access to the high-transverse-momentum region of J/ ψ pair production where model predictions are not yet established. The total and differential cross sections are measured in a phase space defined by the individual J/ ψ transverse momentum ( p T J/ ψ ) and rapidity (| y J/ ψ |): | y J/ ψ | 6.5 GeV/ c ; 1.2 4.5 GeV/ c . The total cross section, assuming unpolarized prompt J/ ψ pair production is 1.49 ± 0.07 (stat) ±0.13 (syst) nb. Different assumptions about the J/ ψ polarization imply modifications to the cross section ranging from −31% to +27%