Georecursos en el entorno de Molina de Aragón (provincia de Guadalajara): una alternativa al aula

La Comarca del Señorío de Molina (Guadalajara) posee una notable geodiversidad y un enorme potencial para el desarrollo de actividades didácticas e interpretativas. En este trabajo se describen algunas actividades basadas en la infraestructura presente y en los valores naturales de la zona, haciendo especial hincapié en la geología y en su papel en la configuración del paisaje. El objetivo principal es meramente didáctico a nivel de universidad. En este sentido se consideran no sólo aspectos geológicos, sino también aspectos de gestión y protección

Crystal Structure of the Hemochromatosis Protein HFE and Characterization of Its Interaction with Transferrin Receptor

AbstractHFE is an MHC-related protein that is mutated in the iron-overload disease hereditary hemochromatosis. HFE binds to transferrin receptor (TfR) and reduces its affinity for iron-loaded transferrin, implicating HFE in iron metabolism. The 2.6 Å crystal structure of HFE reveals the locations of hemochromatosis mutations and a patch of histidines that could be involved in pH-dependent interactions. We also demonstrate that soluble TfR and HFE bind tightly at the basic pH of the cell surface, but not at the acidic pH of intracellular vesicles. TfR:HFE stoichiometry (2:1) differs from TfR:transferrin stoichiometry (2:2), implying a different mode of binding for HFE and transferrin to TfR, consistent with our demonstration that HFE, transferrin, and TfR form a ternary complex

Detection of 4765 MHz OH Emission in a Pre-Planetary Nebula -- CRL 618

Jets and outflows are ubiquitous phenomena in astrophysics, found in our Galaxy in diverse environments, from the formation of stars to late-type stellar objects. We present observations conducted with the 305m Arecibo Telescope of the pre-planetary nebula CRL 618 (Westbrook Nebula) - a well studied late-type star that has developed bipolar jets. The observations resulted in the first detection of 4765 MHz OH in a late-type stellar object. The line was narrow (FWHM ~ 0.6 km/s) and ~40 km/s blueshifted with respect to the systemic velocity, which suggests association with the expanding jets/bullets in CRL 618. We also report non-detection at Arecibo of any other OH transition between 1 and 9 GHz. The non-detections were obtained during the observations in 2008, when the 4765 MHz OH line was first discovered, and also in 2015 when the 4765 MHz OH line was not detected. Our data indicate that the 4765 MHz OH line was a variable maser. Modeling of the 4765 MHz OH detection and non-detection of the other transitions is consistent with the physical conditions expected in CRL 618. The 4765 MHz OH maser could originate from dissociation of H2O by shocks after sublimation of icy objects in this dying carbon-rich stellar system, although other alternatives such as OH in an oxygen-rich circumstellar region associated with a binary companion are also possible.Comment: 16 pages, 2 figure

Herschel / HIFI observations of CO, H2O and NH3 in Mon R2

Context. Mon R2 is the only ultracompact HII region (UCHII) where the associated photon-dominated region (PDR) can be resolved with Herschel. Due to its brightness and proximity, it is the best source to investigate the chemistry and physics of highly UV-irradiated PDRs. Aims. Our goal is to estimate the abundance of H2O and NH3 in this region and investigate their origin. Methods. We present new observations obtained with HIFI and the IRAM-30m telescope. Using a large velocity gradient approach, we model the line intensities and derive an average abundance of H2O and NH3 across the region. Finally, we model the line profiles with a non-local radiative transfer model and compare these results with the abundance predicted by the Meudon PDR code. Results. The variations of the line profiles and intensities indicate complex geometrical and kinematical patterns. The H2O lines present a strong absorption at the ambient velocity and emission in high velocity wings towards the HII region. The spatial distribution of the o-H2^18O line shows that the its emission arises in the PDR surrounding the HII region. By modeling the o-H2^18O emission we derive a mean abundance of o-H2O of ~10^-8 relative to H2. The ortho-H2O abundance is however larger, ~1x10^-7, in the high velocity wings. Possible explanations for this larger abundance include an expanding hot PDR and/or an outflow. Ammonia seems to be present only in the envelope with an average abundance of ~2x10^-9 relative to H2. Conclusions. The Meudon PDR code can account for the measured water abundance in the high velocity gas as long as we assume that it originates from a <1 mag hot expanding layer of the PDR, i.e. that the outflow has only a minor contribution to this emission. To explain the abundances in the rest of the cloud the molecular freeze out and grain surface chemistry would need to be included.Comment: 12 pages, 7 figures, 3 tables. Accepted for publication in A&A. Abstract shortened. Updated references, language editing applied in v

Metal-catalyst-free gas-phase synthesis of long-chain hydrocarbons

Development of sustainable processes for hydrocarbons synthesis is a fundamental challenge in chemistry since these are of unquestionable importance for the production of many essential synthetic chemicals, materials and carbon-based fuels. Current industrial processes rely on non-abundant metal catalysts, temperatures of hundreds of Celsius and pressures of tens of bars. We propose an alternative gas phase process under mild reaction conditions using only atomic carbon, molecular hydrogen and an inert carrier gas. We demonstrate that the presence of CH2 and H radicals leads to efficient C-C chain growth, producing micron-length fibres of unbranched alkanes with an average length distribution between C23-C33. Ab-initio calculations uncover a thermodynamically favourable methylene coupling process on the surface of carbonaceous nanoparticles, which is kinematically facilitated by a trap-and-release mechanism of the reactants and nanoparticles that is confirmed by a steady incompressible flow simulation. This work could lead to future alternative sustainable synthetic routes to critical alkane-based chemicals or fuels

Molecular jets driven by high-mass protostars: a detailed study of the IRAS 20126+4104 jet

We present here an extensive analysis of the protostellar jet driven by IRAS 20126+4104, deriving the kinematical, dynamical, and physical conditions of the H2 gas along the flow. The jet has been investigated by means of near-IR H2 and [FeII] narrow-band imaging, high resolution spectroscopy of the 1-0S(1) line (2.12 um), NIR (0.9-2.5 um) low resolution spectroscopy, along with ISO-SWS and LWS spectra (from 2.4 to 200 um). The flow shows a complex morphology. In addition to the large-scale jet precession presented in previous studies, we detect a small-scale wiggling close to the source, that may indicate the presence of a multiple system. The peak radial velocities of the H2 knots range from -42 to -14 km s^-1 in the blue lobe, and from -8 to 47 km s^-1 in the red lobe. The low resolution spectra are rich in H_2 emission, and relatively faint [FeII] (NIR), [OI] and [CII] (FIR) emission is observed in the region close to the source. A warm H2 gas component has an average excitation temperature that ranges between 2000 K and 2500 K. Additionally, the ISO-SWS spectrum reveals the presence of a cold component (520 K), that strongly contributes to the radiative cooling of the flow and plays a major role in the dynamics of the flow. The estimated L(H2) of the jet is 8.2+/-0.7 L_sun, suggesting that IRAS20126+4104 has an accretion rate significantly increased compared to low-mass YSOs. This is also supported by the derived mass flux rate from the H2 lines (Mflux(H2)~7.5x10^-4 M_sun yr^-1). The comparison between the H2 and the outflow parameters strongly indicates that the jet is driving, at least partially, the outflow. As already found for low-mass protostellar jets, the measured H2 outflow luminosity is tightly related to the source bolometric luminosity.Comment: 16 pages, 12 figures. A&A accepte

Dense Gas in Nearby Galaxies: XVII. The Distribution of Ammonia in NGC253, Maffei2 and IC342

The central few 100 pc of galaxies often contain large amounts of molecular gas. The chemical and physical properties of these extragalactic star formation regions differ from those in galactic disks, but are poorly constrained. This study aims to develop a better knowledge of the spatial distribution and kinetic temperature of the dense neutral gas associated with the nuclear regions of three prototypical spiral galaxies, NGC253, IC342, and Maffei2. VLA CnD and D configuration measurements have been made of three ammonia (NH3) inversion transitions. The (J,K)=(1,1) and (2,2) transitions of NH3 were imaged toward IC342 and Maffei2. The (3,3) transition was imaged toward NGC253. The entire flux obtained from single-antenna measurements is recovered for all three galaxies observed. Derived lower limits to the kinetic temperatures determined for the giant molecular clouds in the centers of these galaxies are between 25 and 50K. There is good agreement between the distributions of NH3 and other H2 tracers, such as rare CO isotopologues or HCN, suggesting that NH3 is representative of the distribution of dense gas. The "Western Peak" in IC342 is seen in the (6,6) line but not in lower transitions, suggesting maser emission in the (6,6) transition.Comment: 13 pages, 8 figures, latex format, accepted by A&

Kinetic temperatures toward X1/X2 orbit interceptions regions and Giant Molecular Loops in the Galactic center region

Context: It is well known that the kinetic temperatures, Tkin, of the molecular clouds in the Galactic center region are higher than in typical disk clouds. However, the Tkin of the molecular complexes found at higher latitudes towards the giant molecular loops in the central region of the Galaxy is so far unknown. The gas of these high latitude molecular clouds (hereafter referred to as halo clouds) is located in a region where the gas in the disk may interact with the gas in the halo in the Galactic center region. Aims: To derive Tkin in the molecular clouds at high latitude and understand the physical process responsible for the heating of the molecular gas both in the Central Molecular Zone (the concentration of molecular gas in the inner 500 pc) and in the giant molecular loops. Methods: We measured the metastable inversion transitions of NH3 from (1,1) to (6,6) toward six positions selected throughout the Galactic central disk and halo. We used rotational diagrams and large velocity gradient modeling to estimate the kinetic temperatures toward all the sources. We also observed other molecules like SiO, HNCO, CS, C34S, C18O, and 13CO, to derive the densities and to trace different physical processes (shocks, photodissociation, dense gas) expected to dominate the heating of the molecular gas. Results: We derive for the first time Tkin of the high latitude clouds interacting with the disk in the Galactic center region. We find high rotational temperatures in all the observed positions. We derive two kinetic temperature components (150 K and 40 K) for the positions in the Central Molecular Zone, and only the warm kinetic temperature component for the clouds toward the giant molecular loops. The fractional abundances derived from the different molecules suggest that shocks provide the main heating mechanism throughout the Galactic center, also at high latitudesComment: accepted for publication in A&A 06/09/201

Binding and uptake of H-ferritin are mediated by human transferrin receptor-1

Ferritin is a spherical molecule composed of 24 subunits of two types, ferritin H chain (FHC) and ferritin L chain (FLC). Ferritin stores iron within cells, but it also circulates and binds specifically and saturably to a variety of cell types. For most cell types, this binding can be mediated by ferritin composed only of FHC (HFt) but not by ferritin composed only of FLC (LFt), indicating that binding of ferritin to cells is mediated by FHC but not FLC. By using expression cloning, we identified human transferrin receptor-1 (TfR1) as an important receptor for HFt with little or no binding to LFt. In vitro, HFt can be precipitated by soluble TfR1, showing that this interaction is not dependent on other proteins. Binding of HFt to TfR1 is partially inhibited by diferric transferrin, but it is hindered little, if at all, by HFE. After binding of HFt to TfR1 on the cell surface, HFt enters both endosomes and lysosomes. TfR1 accounts for most, if not all, of the binding of HFt to mitogen-activated T and B cells, circulating reticulocytes, and all cell lines that we have studied. The demonstration that TfR1 can bind HFt as well as Tf raises the possibility that this dual receptor function may coordinate the processing and use of iron by these iron-binding molecules

A synthetic glycodendropeptide induces methylation changes on regulatory T cells linked to tolerant responses in anaphylactic-mice

IntroductionLipid transfer proteins (LTPs) are allergens found in a wide range of plant-foods. Specifically, Pru p 3, the major allergen of peach, is commonly responsible for severe allergic reactions. The need for new alternatives to conventional food allergy treatments, like restrictive diets, suggests allergen immunotherapy as a promising option. It has been demonstrated that sublingual immunotherapy (SLIT) with synthetic glycodendropeptides, such as D1ManPrup3, containing mannose and Pru p 3 peptides induced tolerance in mice and that the persistence of this effect depends on treatment dose (2nM or 5nM). Moreover, it produces changes associated with differential gene expression and methylation profile of dendritic cells, as well as phenotypical changes in regulatory T cells (Treg). However, there are no works addressing the study of epigenetic changes in terms of methylation in the cell subsets that sustain tolerant responses, Treg. Therefore, in this work, DNA methylation changes in splenic-Treg from Pru p 3 anaphylactic mice were evaluated.MethodsIt was performed by whole genome bisulphite sequencing comparing SLIT-D1ManPrup3 treated mice: tolerant (2nM D1ManPrup3), desensitized (5nM D1ManPrup3), and sensitized but not treated (antigen-only), with anaphylactic mice.ResultsMost of the methylation changes were found in the gene promoters from both SLIT-treated groups, desensitized (1,580) and tolerant (1,576), followed by the antigen-only (1,151) group. Although tolerant and desensitized mice showed a similar number of methylation changes, only 445 genes were shared in both. Remarkably, interesting methylation changes were observed on the promoter regions of critical transcription factors for Treg function like Stat4, Stat5a, Stat5b, Foxp3, and Gata3. In fact, Foxp3 was observed exclusively as hypomethylated in tolerant group, whereas Gata3 was only hypomethylated in the desensitized mice.DiscussionIn conclusion, diverse D1ManPrup3 doses induce different responses (tolerance or desensitization) in mice, which are reflected by differential methylation changes in Tregs