Notes on the ecology and distribution of a water flea complex (Anomopoda, Daphniidae) revealed by new DNA barcodes in the Iberian Peninsula

Available DNA barcodes of freshwater zooplankton are scarce in regions like the Iberian Peninsula, which harbours many rare and endemic species and is considered a hotspot of crustacean biodiversity. Recently, a new species of Ceriodaphnia (Cladocera: Daphniidae) was described using morphological analysis of specimens in the Mediterranean region and molecular data on a single locality in southern Spain. In our study, we detected the presence of the newly discovered taxa and here, we provide new DNA sequences on the barcoding region mitochondrial cytochrome c oxidase I together with ecological information of the recently described Ceriodaphnia smirnovi. Additionally, we built a molecular phylogenetic tree and genetically compared these specimens with previously available mitochondrial DNA sequences and with new sequences of the genus recovered in Iberia. Our data suggest that this morphospecies might contain some cryptic taxa and might be more common than previously thought, occupying temporary to semi-permanent ecosystems, with vegetation and highly variable pH and turbidity conditions. Moreover, the existence of a non-identified clade within our phylogenetic tree requires additional morphological research. Our study highlights the need for further research on microcrustacean biota to better constrain its spatial boundaries, phylogenetic relationship and determine species hiding cryptic diversityDepartamento de Estratigrafía y Paleontologí

Laguna Seca sediments reveal environmental and climate change during the latest Pleistocene and Holocene in Sierra Nevada, southern Iberian Peninsula

This study was supported by the I + D + i projects CGL2013-47038- R, CGL2017-85415-R, PID2019-1049449GB-I00, and PID2021- 125619OB-C21/C22 funded by Ministerio Ciencia e Innovación/Agencia Estatal de Investigación/ 10.13039/501100011033/ and Fondo Europeo de Desarrollo Regional “Una manera de hacer Europa”, I + D + i projects A-RNM-336-UGR20 and P20_00059 of the action “Proyectos I + D + i del Programa Operativo FEDER - Junta de Andalucía - UGR” and the research group RNM-190. This research is part of the project “Thematic Center on Mountain Ecosystem & Remote sensing, Deep learning- AI e-Services University of Granada-Sierra Nevada” (LifeWatch-2019- 10-UGR-01), which has been co-funded by the Ministry of Science and Innovation through the FEDER funds from the Spanish Pluriregional Operational Program 2014-2020 (POPE), LifeWatch-ERIC action line. José Carrión was supported by the I + D + I project PID2019-1049449 GB-I00 funded by MCIN/AEI/ 10.13039/501100011033/ and FEDER “Una manera de hacer Europa” and the fellowship 20788/PI/18 of Fundación Séneca. We thank Javier Jaimez for his help with the core drilling in Laguna Seca and Alejandro Navarro and Aurora Baquera for the sediment sampling. ALA acknowledges the predoctoral fellowship BES- 2018-084293 provide by the MCIN/ AEI/ 10.13039/5011000110 33/. CLB acknowledges the European Union for her Marie Sklodowska-Curie grant agreement number 892487 under Horizon 2020 funds. JC acknowledges the Ministerio de Ciencia e Innovación of the Spanish Government for the grant number FJC2020-044215-I of the Juan de la Cierva Formación postdoctoral program.Sedimentation in most glacial lakes and wetlands in the Sierra Nevada (southern Iberian Peninsula) began after the last deglaciation and since the Younger Dryas (YD)-Early Holocene (EH) transition. Therefore, until now, studies on older sedimentary records were lacking in this alpine area, which limits the paleoenvironmental and paleoclimatic information to the Holocene. In this study, we studied palynomorphs from the alpine record from Laguna Seca (LS), the longest and oldest (∼18,000 cal yr BP = 18 kyr) sedimentary record in the Sierra Nevada to investigate the response of forests and lake environments in the western Mediterranean area to climate changes and human impact during the latest Pleistocene and Holocene. The deepest lake conditions occurred during the last deglaciation, indicated by the occurrence of Pediastrum algae, which showed highest abundances during the Heinrich Stadial 1 (HS1) and Bølling-Allerød (B-A) transition. Xerophyte herbs such as Artemisia, Ephedra, and Amaranthaceae were highest during the late B-A and YD indicating regional aridity. Poaceae (grasses) were maxima in the B-A and EH, probably indicating expansion in the barren areas after deglaciation. Maximum in temperature and humidity during the EH and cooling and aridification in the Middle (MH) and Late Holocene (LH) are indicated by the changes in the abundance of deciduous Quercus and Pinus forest species. Botryococcus algae increased during the Early Holocene, while the rest of the algae almost vanished, which could indicate that the lake became very productive but shallower until 8.2 kyr. The lake level lowered and became seasonal in the Middle-Late Holocene transition, coinciding with the regional climate aridification. Microcharcoal analysis done on the palynological preparations agrees with the vegetation changes, showing maxima in the EH and MH, related with the maximum in regional forest occurrence, and a decrease in the LH when the Mediterranean vegetation, and thus fuel availability, diminished. This record shows evidence of anthropogenic impact in the last centuries by cultivation, reforestation, cattle grazing, enhanced erosion and eutrophication.I + D + i projects CGL2013-47038- R, CGL2017-85415-R, PID2019-1049449GB-I00, and PID2021- 125619OB-C21/C22 funded by Ministerio Ciencia e Innovación/Agencia Estatal de Investigación/ 10.13039/501100011033/ and Fondo Europeo de Desarrollo Regional “Una manera de hacer Europa”I + D + i projects A-RNM-336-UGR20 and P20_00059 of the action “Proyectos I + D + i del Programa Operativo FEDER - Junta de Andalucía - UGR” and the research group RNM-190Ministry of Science and Innovation through the FEDER funds from the Spanish Pluriregional Operational Program 2014-2020 (POPE), LifeWatch-ERIC action lineI + D + I project PID2019-1049449 GB-I00 funded by MCIN/AEI/ 10.13039/501100011033/ and FEDER “Una manera de hacer Europa” and the fellowship 20788/PI/18 of Fundación SénecaPredoctoral fellowship BES- 2018-084293 provide by the MCIN/ AEI/ 10.13039/5011000110 33/European Union for her Marie Sklodowska-Curie grant agreement number 892487 under Horizon 2020 fundsMinisterio de Ciencia e Innovación of the Spanish Government for the grant number FJC2020-044215-I of the Juan de la Cierva Formación postdoctoral progra

A 700-year multiproxy reconstruction on the Argentinian Pampas inferred from the sediments of Laguna Blanca Grande

The Pampean region is a crucial area to obtain sensitive paleoclimatic lacustrine archives due to the presence of shallow environments in a territory non impacted by humans until the last centuries. In this study, we provide a paleoecological reconstruction for the last ca. 700 years based on a multiproxy lacustrine record from Laguna Blanca Grande, in Olavarría (Buenos Aires, Argentina). Our inferences, which were based on sedimentary properties, diatom, cladoceran and ostracod assemblages, offered interesting information about hydroclimatic variability and nutrient increase. Changes in relative abundances on diatoms, specifically on Aulacoseira granulata and Aulacoseira granulata var. angustissima and fragilariods, were used to infer shifts in nutrient conditions. The remainder proxies together indicated small lake level changes. Reconstructed hydroclimatic conditions in Laguna Blanca Grande are consistent with previous paleoecological inferences indicating a humid phase around ca. AD 1450 and progressive drier conditions ca. AD 1530–1900. A flood gate construction and an increase of nutrients in the lake revealed a higher human pressure due to population increase and land-use changes during the last century. Further studies on taxonomy and autecology of microcrustaceans are needed to effectively unlock the information contained in biological proxies from Sudamerican records.Fil: López Blanco, Charo. Escuela Politécnica Nacional; Ecuador. Justus Liebig Universitat Giessen; AlemaniaFil: Rodríguez Abaunza, Gloria Alejandra. Universidad Pedagógica y Tecnológica de Colombia; Colombia. Universidad Nacional Autónoma de México; México. Smithsonian Tropical Research Institute; PanamáFil: Seitz, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; ArgentinaFil: Perez, Laura. Universidad de la República; UruguayFil: Cuña Rodriguez, Carolina Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; ArgentinaFil: Fontana, Sonia Leonor. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. University of Applied Sciences; Alemani

Holocene summer temperature reconstruction based on a chironomid record from Sierra Nevada, southern Spain

This study was supported by projects CGL2013-47038-R, CGL2017-85415-R and PID2021-125619OB-C21/C22, funded by the Ministerio de Ciencia e Innovación of Spain, the Agencia Estatal de Investigación and the Fondo Europeo de Desarrollo Regional FEDER MCIN/AEI/10.13039/501100011033/FEDER, UE”; Junta de Andalucía I + D + i Junta de Andalucía 2020 Retos P-20-00059, UGR-FEDER B-RNM-144-UGR18, UGR-FEDER A-RNM-336-UGR20, Project cofinanced by FEDER and LifeWatch-Eric LifeWatch-2019-10-UGR-01 and the research group RNM-190 (Junta de Andalucía). RSA acknowledges several travel grants from Northern Arizona University to support this work. JC thanks the Ministerio de Ciencia e Innovación of Spain for the Juan de la Cierva Formación postdoctoral fellowship. ALA acknowledges the predoctoral fellowship BES-2018-084293 provided by the MCIN/AEI/10.13039/501100011033/. CLB acknowledges the European Union for her Marie Sklodowska-Curie grant agreement number 892487 under Horizon 2020 funds. LJ acknowledges the Ministry of Universities of Spain for her Margarita Salas grant (MS2021-204) financed by the European Union -Next Generation EU funds.Obtaining accurate temperature reconstructions from the past is crucial in understanding the consequences of changes in external climate forcings, such as orbital-scale insolation or multidecadal to centennial-scale variability on the climate system and the environment. In addition, these reconstructions help in comprehending the amplitude of natural temperature changes in the past, which can assist in evaluating the amplitude and rate of recent anthropogenic global warming. Here we present the first detailed Holocene mean July air temperature reconstruction based on chironomid assemblages from sediments retrieved from Laguna de Río Seco, an alpine lake in Sierra Nevada, southern Spain. Coldest climate conditions are recorded during the last glacial maximum and the last deglaciation. Warming occurred in the Early Holocene and warmest summer temperature conditions and the Holocene thermal maximum (HTM) occurred in the interval roughly between 9000 and 7200 cal yr BP, concurrent with summer insolation maxima. Rapid cooling of ∼1.5 °C occurred after the warmest maximum and between ∼7200 and 6500 cal yr BP, and temperatures stabilized between ∼6500 and 3000 cal yr BP. A further cooling began ∼3000 cal yr BP and culminated with coldest summer conditions during the Dark Ages (DA) and Little Ice Age (LIA) at ∼1550 cal yr BP (∼400 CE) and ∼200 cal yr BP (∼1750 CE), respectively. This cooling temperature trend was interrupted by warmer conditions during the Iberian-Roman Humid Period (IRHP) ∼2000 cal yr BP and during the Medieval Climate Anomaly (MCA) at ∼1000 cal yr BP. Our reconstruction shows a greater than two-degree cooling during the Middle and Late Holocene, agreeing with global mean surface temperature (GMST) reconstructions. Modern climate warming (MCW) during summer exceeds the two-degree Celsius forecasted for the future due to anthropogenic greenhouse gases, suggesting that recent warming is amplified at high elevations. Alpine environments and the biodiversity contained there are thus in danger if the observed temperature trend continues in the next decades.Ministerio de Ciencia e Innovación of Spain CGL2013-47038-R, CGL2017-85415-R, PID2021-125619OB-C21/C22Agencia Estatal de InvestigaciónFondo Europeo de Desarrollo Regional FEDER MCIN/AEI/10.13039/501100011033/FEDER, UEJunta de Andalucía P-20-00059, UGR-FEDER B-RNM-144-UGR18, UGR-FEDER A-RNM-336-UGR20FEDERLifeWatch-Eric LifeWatch-2019-10-UGR-01Research group RNM-190 (Junta de Andalucía)Northern Arizona UniversityMinisterio de Ciencia e Innovación of SpainMCIN/AEI/10.13039/501100011033/ BES-2018-084293Horizon 2020 European Union Marie Sklodowska-Curie 892487European Union -Next Generation EU funds MS2021-20

Effect of Temperature on the Size of Sedimentary Remains of Littoral Chydorids

The body size of aquatic invertebrates is, to a great extent, dependent on ambient temperature, but size distributions are also determined by other factors like food supply and predation. The effect of temperature on organisms is formulated in the temperature–size hypothesis, which predicts a smaller body size with increasing temperature. In this study, the effect of temperature on the subfossil remains of three littoral Cladocera (Alona affnis, A. quadrangularis, and Chydorus cf. sphaericus) was investigated. Exoskeletal remains of these species can be found in large numbers in lacustrine sediments and over a wide north–south range in Europe. The total length of both headshield and postabdomen for A. affinis and A. quadrangularis and carapace length for C. cf. sphaericus were measured to observe their response to changes in latitude and temperature. A different response to ambient temperature in the growth of body parts was observed. The size of the headshields of both Alona species and of the carapace of Chydorus was significantly larger in colder regions as opposed to warm ones. It turned out that the postabdomen was not a good predictor of ambient temperature. While the sizes of all remains increased with latitude, the sizes of the Alona remains was smaller in the mountain lakes of the Southern Carpathians than in other cold lakes, in this case in Finland, a fact indicative of the importance of other factors on size distribution. This study demonstrates that a morphological response to climate is present in littoral cladocerans, and, therefore, changes in the length of headshield and carapace may be used as a proxy for climate changes in paleolimnological records

Genetics of immunoglobulin-A vasculitis (Henoch-Schönlein purpura): An updated review

Immunoglobulin-A vasculitis (IgAV) is classically a childhood small-sized blood vessel vasculitis with predominant involvement of the skin. Gastrointestinal and joint manifestations are common in patients diagnosed with this condition. Nephritis, which is more severe in adults, constitutes the most feared complication of this vasculitis. The molecular bases underlying the origin of IgAV have not been completely elucidated. Nevertheless, several pieces of evidence support the claim that genes play a crucial role in the pathogenesis of this disease. The human leukocyte antigen (HLA) region is, until now, the main genetic factor associated with IgAV pathogenesis. Besides a strong association with HLA class II alleles, specifically HLA-DRB1 alleles, HLA class I alleles also seem to influence on the predisposition of this disease. Other gene polymorphisms located outside the HLA region, including those coding cytokines, chemokines, adhesion molecules as well as those related to T-cells, aberrant glycosylation of IgA1, nitric oxide production, neoangiogenesis, renin-angiotensin system and lipid, Pyrin and homocysteine metabolism, may be implicated not only in the predisposition to IgAV but also in its severity. An update of the current knowledge of the genetic component associated with the pathogenesis of IgAV is detailed in this review.Acknowledgements: RL-Mis supported by the Miguel Servet I programme of the Spanish Ministry of Economy and Competitiveness through the grant CP16/ 00033. FG is recipient of a Sara Borrell postdoctoral fellowship from the “Instituto Carlos III de Salud” at the Spanish Ministry of Health (Spain) (CD15/00095). SR-M is supported by funds from the RETICS Program (RIER) (RD16/0012/0009). FDC is supported by the Ramón y Cajal programme of the Spanish Ministry of Economy and Competitiveness through the grant RYC-2014-16458

Multiproxy study in two karstic lakes in the Iberian Ranges: climate variability and anthropogenic activities during the last millennium

Resumen de Tesis doctoral europea, Departamento de Microbiología y Ecología. Universidad de Valencia, Abril 2012, Dirección: María Rosa Miracle y Eduardo Vicente

А new species of Alona Baird, 1843 (Cladocera: Chydoridae) from the ancient Lake Ohrid

Sinev, Artem Y., López-Blanco, Charo (2018): А new species of Alona Baird, 1843 (Cladocera: Chydoridae) from the ancient Lake Ohrid. Zootaxa 4526 (4): 434-446, DOI: https://doi.org/10.11646/zootaxa.4526.4.

FIGURE 1 in А new species of Alona Baird, 1843 (Cladocera: Chydoridae) from the ancient Lake Ohrid

FIGURE 1. Alona begoniae sp. nov. from St Naum Bay, Lake Ohrid, Republic of North Macedonia (type locality). A, juvenile female of instar II. B–I, adult parthenogenetic female. B, lateral view. C, ventral margin of valves. D, posteroventral angle of valves. E, head shield. F, head pores. G–H, postabdomen. I, antenna. J, ephippial female. K–M, adult male. K, lateral view. L, postabdomen. M, postabdominal claw

First steps towards a barcoding database for the Iberian cladocerans

Due to similarities in morphological features together with strong dispersal abilities, it was thought that some groups of zooplankton (e.g. rotifers, copepods, and cladocerans) have cosmopolitan distributions. In the particular case of cladocerans, recent molecular studies using DNA barcode regions have indicated a different picture, including the existence of multiple regional endemic species and geographical phylogroups; even at very small geographical scales. This has demostrated that cladocera species are less widely distributed than assumed. Morphological identifications of these animals require expertise and high taxonomic specialization. Even so, species identifications are hampered by the small size of the organisms (especially from the littoral zone) and by the sampling cost for obtaining rare species and both parthenogenetic and gamogenetic specimens. The use of molecular techniques can provide new tools to identify cryptic diversity, and by being added to taxonomical approaches, provide more precise data of biodiversity. However, the accuracy of species assignment in metabarcoding relies on the availability of a DNA reference library, which is challenging in areas with high endemicity rates such as the Iberian Peninsula. A preliminary compilation of the available molecular data for the cytochrome (COI) in public repositories (Barcode of Life Data Systems and NCBI GenBank) shows that the available sequences only cover ~60% of the Iberian freshwater cladocerans. The family Daphniidae is very well represented, while the family Chydoridae, which contained most of the Iberian endemism, is underrepresented. We have identified the gaps, and are now focusing on collecting the target organisms to fill the missing taxa of the Iberian library. A compendium of the sampling points, species recovered so far, pitfalls, and future strategy is presented here. The effective completion of a DNA database for cladocerans will have applications not only in biomonitoring programs but to develop DNA-based methods in paleolimnology