Screening of efficient rhizobacteria associated with cauliflower (Brassica oleraceavar. botrytis L.) for plant growth promoting traits

In the current study, a total of 25 isolates were isolated from the rhizosphere and roots of cauliflower (Brassica oleraceavar. botrytis L.) from the vicinity of Una district of Himachal Pradesh. The isolates were tested in vitro for their ability to solubilise phosphorous and produce siderophore, indole acetic acid (IAA), hydrogen cyanide (HCN) and antifungal metabolites against the soil borne pathogens. Results revealed that out of 25, only 4 rhizospheric isolates (SB5, SB11, SB8 and SB10) have maximum plant growth promoting attributes. The isolates were identified as Bacillus sp. on the basis of Bergey’s manual of systematic bacteriology. The isolate SB11 recorded highest phosphate solubilizing efficiency in solid medium (109.09%) and in liquid medium (350?g/ml). Maximum production of IAA (51.96?g/ml), siderophore (91.41%) and HCN were also observed for the same isolate. Further-more, the isolate SB11 produced highest antifungal metabolite production against Rhizoctoniasolani(37.11%), Sclerotiniasclerotiorum(41.11%), and Pythium sp. (71.11%) causing root rot, stalk rot and damping off diseases in cauliflower, respectively. The selected isolate (SB11) showed optimum growth at a pH of 7.0, 35°C temperature and 2% NaCl. On the basis of multifarious PGP-traits the SB11 isolate has tremendous potential to be used as a bioferti-lizer/bioprotectant for growth promotion and natural protection of cauliflower under low hill conditions of Himachal Pradesh

Efficacy and safety of lamotrigine in patients with trigeminal neuralgia: a comparison with carbamazepine

Background: Antiepileptic drugs are increasingly used in the treatment of pain syndromes. This study evaluates efficacy and safety of lamotrigine in comparison with carbamazepine (CBZ) in trigeminal neuralgia (TN) patients.Methods: A total of 50 previously and newly diagnosed patients of TN were administered with lamotrigine (LTG) in comparison to CBZ. The final titration in dose for LTG was 400 mg/day and 1200 mg/day for CBZ. Clinical assessment (pain relief) was done before and after 15, 30, and 60 days of drug administration by visual analog scale and verbal rating scale. Side effects were recorded during study in both groups.Results: Of 25 patients in Group I who received CBZ, 16 patients (64%) experienced complete pain relief, while in Group II (LTG), 21 patients experienced complete pain relief. LTG was generally well tolerated except one patient was withdrawn due to skin rashes. CBZ was associated with mild hematological and central nervous system side effects.Conclusion: LTG is as effective and safe treatment for the management of TN as compared to CBZ

Comparative evaluation of short-term versus long-term post-operative antibiotic prophylaxis after mandibular fractures

Background: No consensus is present among the clinicians regarding the use of post-operative antibiotics after the open reduction and internal fixation. This study evaluates the efficacy of short-term (48 hrs) versus long-term (5 days) post-operative antibiotics in preventing infection rates after the open reduction and internal fixation in mandible fractures.Methods: A total of 80 patients of dentate mandibular fractures were divided randomly in 2 groups; Group 1 received post-operative intravenous antibiotics (amoxicillin+clavulanate 1.2 g+ metronidazole 0.5 g/100 cc) TDS for 48 hrs post-operatively Group 2 received same course for 5 post-operative days and patient were monitored for the signs of infection according to the centers for disease control and prevention criteria, rise in white blood cells counts and C-reactive protein (CRP) levels. Side effects were recorded in both groups.Results: Out of 39 patients in Group 1 two patients showed rise in CRP levels and single patient developed abscess, whereas in Group 2 wound dehiscence was noted in 2 patients and both had prolonged rise in CRP levels at the 5th day.Conclusions: No significant difference in post-operative infection rates was seen between two groups which suggests short-term post-operative antibiotic treatment is equally efficacious as long-term treatment

Cathepsin B cysteine protease gene is upregulated during leaf senescence and exhibits differential expression behavior in response to phytohormones in Picrorhiza kurrooa Royle ex Benth.

AbstractMedicinal importance of Picrorhiza (Picrorhiza kurrooa Royle ex Benth — an herb of western Himalayan region) and its endangered status in Red Data Book presses an urgent need for intensive R&D interventions towards ensuring its availability for the medicinal use, its sustainability and improvement. The present study was conducted on cathepsin B cysteine protease in Picrorhiza. Cathepsin B cysteine protease has been reported to function in diverse processes such as senescence, abscission, programmed cell death, fruit ripening and in response to pathogen and pest attacks. A full-length cDNA-Pk-cbcp encoding cathepsin B-like cysteine protease was cloned from Picrorhiza. The full length Pk-cbcp cDNA consisted of 1369bp with an open reading frame of 1080bp, 80bp 5′ untranslated region and 209bp 3′ untranslated region. The deduced Pk-cbcp protein contained 359 amino acids with a molecular weight of 39.981kDa and an isoelectric point of 5.75. Secondary structure analysis revealed that Pk-cbcp had 28.97% α-helices, 14.48% β-turns, 19.50% extended strands and 37.05% random coils. Semi-quantitative PCR analysis revealed 157% higher expression of Pk-cbcp during senescence compared to that of pre-senescence. Further, application of phytohormones abscisic acid, jasmonic acid and cytokinin influenced the temporal expression status of Pk-cbcp. Abscisic acid and jasmonic acid increased the expression level whereas cytokinin reduced the expression. The findings suggest the role of Pk-cbcp in leaf senescence in Picrorhiza which may be differentially mediated through phytohormones

Study of pregnancy outcome in women with cardiac disease: a retrospective analysis

Background: Cardiac disease complicates 1% of all pregnancies. It is one of the 3 major indirect causes of maternal mortality in India. Objective of the study is to evaluate the maternal and fetal outcome in patients with cardiac disease in pregnancy.Methods: A retrospective analysis was carried out in 117 pregnant women with known or newly diagnosed heart disease from January 2013 to December 2015 at a tertiary care hospital.Results: In the present study, the incidence of cardiac disease was 0.8%. Majority 66 (56.4%) were of 23-27 years of age, 72 (61.5%) were primigravida. Mostly 84 (71.8%) belonging to New York Heart Association (NYHA) Class I and II. Rheumatic heart disease seen in 96 (82.1%) and congenital heart disease in 17.9% subjects. Among RHD Mitral stenosis was the most common, seen in 51 (53.1%) cases. Majority 78 (66.6%) had vaginal delivery and caesarean section in 28 (23.9%) cases. Most common maternal complication was anaemia seen in 54 (46.2%) cases, congestive cardiac failure complicated 23 (19.6%) cases. 18 patients required ICU care and maternal mortality in 6 cases (5.12%). The live births in (94.8%) cases, 33 (28.2%) babies required NICU admission and perinatal mortality was 7.7%.Conclusions: Heart disease in pregnancy is a high risk condition and has a major impact on pregnancy outcome. Rheumatic heart disease being the prominent cardiac lesion. Fetomaternal mortality and morbidity can be reduced with proper antenatal, intrapartum and postnatal care in conjunction with cardiologist and neonatologist

Effect of Antimicrobial Photodynamic Therapy as an Adjunct to Nonsurgical Treatment of Deep Periodontal Pockets: A Clinical Study

Introduction: Deep periodontal pockets pose a great challenge for nonsurgical periodontal treatment. Scaling and root planing (SRP) alone may not suffice in cases where surgical therapy cannot be undertaken. Various recent studies have suggested the use of antimicrobial Photodynamic Therapy (aPDT) for the management of periodontal infections. The aim of this study was to evaluate the effects of using aPDT along with SRP, compared to SRP alone for the management of deep periodontal pockets.Methods: Thirty patients with chronic periodontitis, who met the criteria of having periodontal pockets with depth ≥ 6 mm and bleeding on probing (BOP) in at least 2 different quadrants were included. After SRP, one quadrant was randomly selected for aPDT (test), while another served as control. Clinical parameters i.e. plaque index (PI), modified sulcular bleeding index (mSBI), probing depth (PD) and clinical attachment level (CAL) were measured at baseline, 1 month and 3 months post-treatment intervals.Results: All clinical parameters significantly improved in both groups after 1 and 3 months. At 1-month interval, inter-group difference in mean change was statistically significant (P < 0.05) in terms of mSBI (0.85 ± 0.41in test vs 0.54 ± 0.47 in control group) and PD (1.77±0.86 in test vs 1.3 ± 0.95 in control group). At 3 months interval, no statistically significant difference was observed between test and control groups except in terms of mSBI (0.97 ± 0.45 in test vs 0.73 ± 0.42 in control group).Conclusion: aPDT appears to play an additional role in reduction of gingival inflammation when used along with nonsurgical mechanical debridement of deep periodontal pockets

Effect on soil health of cauliflower (Brassica oleracea var. botrytis) cultivation with Integrated Nutrient Management

Brassica oleracea var. botrytis L. (Cauliflower) is an important commercial vegetable crop grown all over the world. In order to meet the growing demand of burgeoning population, large amounts of herbicides, pesticides and fertilizers are being applied to the fields every year to achieve maximum production leading to deleterious environmental effects.The present investigation ‘Integrated nutrient management in cauliflower was undertaken at the Instructional Farm of Department of Horticulture, College of Agriculture, Latur during the Rabi season. The experiment laid out in Randomized Block Design (R.B.D.) with fourteen treatments replicated thrice. The treatment comprises with RDF (120:80:40 NPK kg/ha.), FYM (10 t/ha), Azotobacter and Azospirillum (10 kg/ha). The variety Snowball – 16 was selected for the study. The data regarding soil analysis after harvesting, the maximum available nitrogen (265.66 kg ha-1), available phosphorus (23.26 kg ha-1) and available potassium (415.33 kg ha-1) were recorded in highest dose of INM i.e. 100 % RDF + FYM + Azotobacter + Azospirillum(T2) and is was statistically at par with T6 and T8. The maximum organic carbon (0.93 per cent) was recorded in the treatment 75 % RDF + FYM + Azotobacter + Azospirillum(T6). There were no significant differences of INM treatments observed on soil pH and electrical conductivity. This result suggested that 25% chemical fertilizers can be reduced without any compromise on fertility status of the soil for cauliflower crop production in sub-tropical condition

Awareness, attitude and practice of family planning methods in a tertiary care hospital, Uttar Pradesh, India

Background: Family planning services have the potential to improve the quality of the lives of people and their economic welfare. The objective of the study was to assess the level of awareness, attitude, and current practice of different family planning methods and to elicit reasons for couples not using any method.Methods: A cross sectional descriptive study was done among 1050 married women of reproductive age .Results: Most of the women were between 21-34 years of age (60.1%) and had primary level of education (40%).It was observed that with increase in level of education, awareness also increased (77.7%). The most common source of information was mass media (53.2%). Contraceptive prevalence rate was 62.9%, higher than the national data as 28.5%. Most of them (93.1%) were aware of atleast one family planning method. The most commonly known were OCPs (74.8%), condom (68.8%) and IUCD (56.6%). Awareness about female sterilisation (36.4%) was more than male sterilisation (25.3%). 62.9% had used atleast one contraceptive method, three prevailing methods used were condom (65.1%), OCPs (31.8%) and IUCD (9.09%). Reasons precluding women from practicing contraception were desire to have a child (60.5%), lack of knowledge (42.4%), and unbearable side effects (25.5%). Majority (92.4%) thought that contraceptive use was beneficial but only (27.2%) expressed the willingness to start practicing contraception if they received more information about the subject.Conclusions: Majority of women had optimal knowledge and favourable attitude, still there is need to educate and motivate couples to improve family planning services

Differential cross section measurements for the production of a W boson in association with jets in proton–proton collisions at √s = 7 TeV

Measurements are reported of differential cross sections for the production of a W boson, which decays into a muon and a neutrino, in association with jets, as a function of several variables, including the transverse momenta (pT) and pseudorapidities of the four leading jets, the scalar sum of jet transverse momenta (HT), and the difference in azimuthal angle between the directions of each jet and the muon. The data sample of pp collisions at a centre-of-mass energy of 7 TeV was collected with the CMS detector at the LHC and corresponds to an integrated luminosity of 5.0 fb[superscript −1]. The measured cross sections are compared to predictions from Monte Carlo generators, MadGraph + pythia and sherpa, and to next-to-leading-order calculations from BlackHat + sherpa. The differential cross sections are found to be in agreement with the predictions, apart from the pT distributions of the leading jets at high pT values, the distributions of the HT at high-HT and low jet multiplicity, and the distribution of the difference in azimuthal angle between the leading jet and the muon at low values.United States. Dept. of EnergyNational Science Foundation (U.S.)Alfred P. Sloan Foundatio

Penilaian Kinerja Keuangan Koperasi di Kabupaten Pelalawan

This paper describe development and financial performance of cooperative in District Pelalawan among 2007 - 2008. Studies on primary and secondary cooperative in 12 sub-districts. Method in this stady use performance measuring of productivity, efficiency, growth, liquidity, and solvability of cooperative. Productivity of cooperative in Pelalawan was highly but efficiency still low. Profit and income were highly, even liquidity of cooperative very high, and solvability was good