73 research outputs found
Renal Transplant Immunosuppression Impairs Natural Killer Cell Function In Vitro and In Vivo
Background: Despite an increasing awareness of the importance of innate immunity, the roles of natural killer (NK) cells in transplant rejection and antiviral and cancer immunity during immunosuppression have not been clearly defined. Methods: To address this issue we have developed a quantitative assay of NK cell function that can be used on clinical samples and have studied the influence of immunosuppression on NK cell function. NK cell degranulation and intracellular interferon (IFN)-c production were determined by flow cytometry of peripheral blood samples. Results: Overnight ex vivo treatment of peripheral blood cells from healthy controls with ciclosporin or tacrolimus inhibited NK cell degranulation and IFN-c production in a dose-dependent manner. A similar impairment of function was seen in NK cells from patients treated in vivo with calcineurin inhibitors. In the early post-transplant period, there was a variable reduction of NK cell counts after treatment with alemtuzumab and basiliximab. Conclusions: The functional inhibition of NK cells in early transplant patients coincides with the period of maximum susceptibility to viral infections. The ability to assay NK cell function in clinical samples allows assessment of the impact of immunosuppressio
Practical Recommendations for Long-term Management of Modifiable Risks in Kidney and Liver Transplant Recipients
Application of 3 mg/kg of Cyclosporine A (NEORAL) Once Daily is Effective for Severe and Moderate Psoriasis
Single Daily Ciclosporin (Neoral) Administration before Breakfast for Psoriasis Treatment
A case report of a renal transplant recipient developing chronic glomerular rejection with a weak antibody against anti-donor T-cell, only detected by flow-cytometry crossmatch
Buccal vs. nasogastric tube administration of tacrolimus after pediatric liver transplantation
Lessons From Routine Dose Adjustment of Tacrolimus in Renal Transplant Patients Based on Global Exposure
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