899 research outputs found
A novel alveolar Krebs cycle-triggered CO2 sensing mechanism regulates regional pulmonary ventilation.
Pulmonary perfusion disorders provoke atelectasis in order to minimize ventilation/perfusion mismatch. However, the underlying mechanisms remain unknown. Because intraalveolar CO2 concentration ([CO2]iA) declines as a consequence of poor pulmonary perfusion we postulated the existence a novel alveolar CO2-sensing mechanism which adapts the ventilation to perfusion. Real-time fluorescence imaging of rat lungs revealed that low [CO2]iA decreased cytosolic and increased mitochondrial Ca2+ in alveolar epithelial cells (AEC), leading to reduction of surfactant secretion and alveolar ventilation. Mitochondrial inhibition by ruthenium red or rotenone blocked the hypocapnia-induced responses. In cultured Type 2 AEC hypocapnia decreased cytosolic Ca2+ independently from pH and increased the NADH production, the mitochondrial transmembrane potential, and subsequently the mitochondrial Ca2+ uptake. All responses were completely blocked by the gene knockdown of the NADH producing Krebs cycle enzyme isocitrate dehydrogenase. Furthermore, ligature of the pulmonary artery of rabbits decreased alveolar ventilation, surfactant secretion, and lung compliance. Addition of 5% CO2 to the inspiratory gas inhibited all responses. Accordingly, we provide evidence for a novel CO2-sensing mechanism of AEC regulated by the Krebs cycle activity in terms of a negative feedback loop adapting surfactant secretion and thus regional ventilation to pulmonary perfusion
Predicting forest cover in distinct ecosystems: the potential of multi-source sentinel-1 and -2 data fusion
The fusion of microwave and optical data sets is expected to provide great potential for the
derivation of forest cover around the globe. As Sentinel-1 and Sentinel-2 are now both operating
in twin mode, they can provide an unprecedented data source to build dense spatial and temporal
high-resolution time series across a variety of wavelengths. This study investigates (i) the ability of
the individual sensors and (ii) their joint potential to delineate forest cover for study sites in two
highly varied landscapes located in Germany (temperate dense mixed forests) and South Africa (open
savanna woody vegetation and forest plantations). We used multi-temporal Sentinel-1 and single
time steps of Sentinel-2 data in combination to derive accurate forest/non-forest (FNF) information via
machine-learning classifiers. The forest classification accuracies were 90.9% and 93.2% for South Africa
and Thuringia, respectively, estimated while using autocorrelation corrected spatial cross-validation
(CV) for the fused data set. Sentinel-1 only classifications provided the lowest overall accuracy of
87.5%, while Sentinel-2 based classifications led to higher accuracies of 91.9%. Sentinel-2 short-wave
infrared (SWIR) channels, biophysical parameters (Leaf Area Index (LAI), and Fraction of Absorbed
Photosynthetically Active Radiation (FAPAR)) and the lower spectrum of the Sentinel-1 synthetic
aperture radar (SAR) time series were found to be most distinctive in the detection of forest cover.
In contrast to homogenous forests sites, Sentinel-1 time series information improved forest cover
predictions in open savanna-like environments with heterogeneous regional features. The presented
approach proved to be robust and it displayed the benefit of fusing optical and SAR data at high
spatial resolution
Role of P-selectin in platelet sequestration in pulmonary capillaries during endotoxemia
Background: There is growing evidence that platelets accumulate in the lung and contribute to the pathogenesis of acute lung injury during endotoxemia. The aims of the present study were to localize platelet sequestration in the pulmonary microcirculation and to investigate the role of P-selectin as a molecular mechanism of platelet endothelial cell interaction. Methods: We used in vivo fluorescence microscopy to quantify the kinetics of fluorescently labeled erythrocytes and platelets in alveolar capillary networks in rabbit lungs. Results: Six hours after onset of endotoxin infusion we observed a massive rolling along and firm adherence of platelets to lung capillary endothelial cells whereas under control conditions no platelet sequestration was detected. P-selectin was expressed on the surface of separated platelets which were incubated with endotoxin and in lung tissue. Pretreatment of platelets with fucoidin, a P-selectin antagonist, significantly attenuated the endotoxin-induced platelet rolling and adherence. In contrast, intravenous infusion of fucoidin in endotoxin-treated rabbits did not inhibit platelet sequestration in pulmonary capillaries. Conclusion: We conclude that platelets accumulate in alveolar capillaries following endotoxemia. P-selectin expressed on the surface of platelets seems to play an important role in mediating this platelet-endothelial cell interaction. Copyright (c) 2006 S. Karger AG, Basel
Multiple recombination events between two cytochrome P450 loci contribute to global pyrethroid resistance in Helicoverpa armigera
The cotton bollworm, Helicoverpa armigera (Hübner) is one of the most serious insect pest species to evolve resistance against many insecticides from different chemical classes. This species has evolved resistance to the pyrethroid insecticides across its native range and is becoming a truly global pest after establishing in South America and having been recently recorded in North America. A chimeric cytochrome P450 gene, CYP337B3, has been identified as a resistance mechanism for resistance to fenvalerate and cypermethrin. Here we show that this resistance mechanism is common around the world with at least eight different alleles. It is present in South America and has probably introgressed into its closely related native sibling species, Helicoverpa zea. The different alleles of CYP337B3 are likely to have arisen independently in different geographic locations from selection on existing diversity. The alleles found in Brazil are those most commonly found in Asia, suggesting a potential origin for the incursion of H. armigera into the Americas
Assessment of terrain elevation estimates from ICESat-2 and GEDI spaceborne LiDAR missions across different land cover and forest types
Accurate measurements of terrain elevation are crucial for many ecological applications. In this study, we sought
to assess new global three-dimensional Earth observation data acquired by the spaceborne Light Detection and
Ranging (LiDAR) missions Ice, Cloud, and Land Elevation Satellite-2 (ICESat-2) and Global Ecosystem Dynamics
Investigation (GEDI). For this, we examined the “ATLAS/ICESat-2 L3A Land and Vegetation Height”, version 5
(20 × 14 m and 100 × 14 m segments) and the “GEDI Level 2A Footprint Elevation and Height Metrics”, version
2 (25 m circle). We conducted our analysis across four land cover classes (bare soil, herbaceous, forest, savanna),
and six forest types (temperate broad-leaved, temperate needle-leaved, temperate mixed, tropical upland,
tropical floodplain, and tropical secondary forest). For assessment of terrain elevation estimates from spaceborne
LiDAR data we used high resolution airborne data. Our results indicate that both LiDAR missions provide accurate terrain elevation estimates across different land cover classes and forest types with mean error less than 1
m, except in tropical forests. However, using a GEDI algorithm with a lower signal end threshold (e.g., algorithm
5) can improve the accuracy of terrain elevation estimates for tropical upland forests. Specific environmental
parameters (terrain slope, canopy height and canopy cover) and sensor parameters (GEDI degrade flags, terrain
estimation algorithm; ICESat-2 number of terrain photons, terrain uncertainty) can be applied to improve the
accuracy of ICESat-2 and GEDI-based terrain estimates. Although the goodness-of-fit statistics from the two
spaceborne LiDARs are not directly comparable since they possess different footprint sizes (100 × 14 m segment
or 20 × 14 m segment vs. 25 m circle), we observed similar trends on the impact of terrain slope, canopy cover
and canopy height for both sensors. Terrain slope strongly impacts the accuracy of both ICESat-2 and GEDI
terrain elevation estimates for both forested and non-forested areas. In the case of GEDI the impact of slope is,
however, partly caused by horizontal geolocation error. Moreover, dense canopies (i.e., canopy cover higher than
90%) affect the accuracy of spaceborne LiDAR terrain estimates, while canopy height does not, when considering
samples over flat terrains. Our analysis of the accuracy and precision of current versions of spaceborne LiDAR
products for different vegetation types and environmental conditions provides insights on parameter selection
and estimated uncertainty to inform users of these key global datasets
Recommended from our members
Multiple recombination events between two cytochrome P450 loci contribute to global pyrethroid resistance in Helicoverpa armigera
The cotton bollworm, Helicoverpa armigera (Hubner) is one of the most serious insect pest species to evolve resistance against many insecticides from different chemical classes. This species has evolved resistance to the pyrethroid insecticides across its native range and is becoming a truly global pest after establishing in South America and having been recently recorded in North America. A chimeric cytochrome P450 gene, CYP337B3, has been identified as a resistance mechanism for resistance to fenvalerate and cypermethrin. Here we show that this resistance mechanism is common around the world with at least eight different alleles. It is present in South America and has probably introgressed into its closely related native sibling species, Helicoverpa zea. The different alleles of CYP337B3 are likely to have arisen independently in different geographic locations from selection on existing diversity. The alleles found in Brazil are those most commonly found in Asia, suggesting a potential origin for the incursion of H. armigera into the Americas
A heterozygous moth genome provides insights into herbivory and detoxification
How an insect evolves to become a successful herbivore is of profound biological and practical importance. Herbivores are often adapted to feed on a specific group of evolutionarily and biochemically related host plants1, but the genetic and molecular bases for adaptation to plant defense compounds remain poorly understood2. We report the first whole-genome sequence of a basal lepidopteran species, Plutella xylostella, which contains 18,071 protein-coding and 1,412 unique genes with an expansion of gene families associated with perception and the detoxification of plant defense compounds. A recent expansion of retrotransposons near detoxification-related genes and a wider system used in the metabolism of plant defense compounds are shown to also be involved in the development of insecticide resistance. This work shows the genetic and molecular bases for the evolutionary success of this worldwide herbivore and offers wider insights into insect adaptation to plant feeding, as well as opening avenues for more sustainable pest management.Minsheng You … Simon W Baxter … et al
Azimuthal anisotropy of charged jet production in root s(NN)=2.76 TeV Pb-Pb collisions
We present measurements of the azimuthal dependence of charged jet production in central and semi-central root s(NN) = 2.76 TeV Pb-Pb collisions with respect to the second harmonic event plane, quantified as nu(ch)(2) (jet). Jet finding is performed employing the anti-k(T) algorithm with a resolution parameter R = 0.2 using charged tracks from the ALICE tracking system. The contribution of the azimuthal anisotropy of the underlying event is taken into account event-by-event. The remaining (statistical) region-to-region fluctuations are removed on an ensemble basis by unfolding the jet spectra for different event plane orientations independently. Significant non-zero nu(ch)(2) (jet) is observed in semi-central collisions (30-50% centrality) for 20 <p(T)(ch) (jet) <90 GeV/c. The azimuthal dependence of the charged jet production is similar to the dependence observed for jets comprising both charged and neutral fragments, and compatible with measurements of the nu(2) of single charged particles at high p(T). Good agreement between the data and predictions from JEWEL, an event generator simulating parton shower evolution in the presence of a dense QCD medium, is found in semi-central collisions. (C) 2015 CERN for the benefit of the ALICE Collaboration. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).Peer reviewe
- …