Chatting about the unaccepted: Self-disclosure of unaccepted news exposure behaviour to a chatbot

Conversational technologies such as chatbots have shown to be promising in eliciting self-disclosure in several contexts. Implementing such a technology that fosters self-disclosure can help to assess sensitive topics such as behaviours that are perceived as unaccepted by others, i.e. the exposure to unaccepted (alternative) news sources. This study tests whether a conversational (chatbot) format, compared to a traditional web-based survey, can enhance self-disclosure in the political news context by implementing a two-week longitudinal, experimental research design (n = 193). Results show that users disclose unaccepted news exposure significantly more often to a chatbot, compared to a traditional web-based survey, providing evidence for a chatbots’ ability to foster the disclosure of sensitive behaviours. Unlike our hypotheses, our study also shows that social presence, intimacy, and enjoyment cannot explain self-disclosure in this context, and that self-disclosure generally decreases over time

MitoLoc: A method for the simultaneous quantification of mitochondrial network morphology and membrane potential in single cells.

Mitochondria assemble into flexible networks. Here we present a simple method for the simultaneous quantification of mitochondrial membrane potential and network morphology that is based on computational co-localisation analysis of differentially imported fluorescent marker proteins. Established in, but not restricted to, Saccharomyces cerevisiae, MitoLoc reproducibly measures changes in membrane potential induced by the uncoupling agent CCCP, by oxidative stress, in respiratory deficient cells, and in ∆fzo1, ∆ref2, and ∆dnm1 mutants that possess fission and fusion defects. In combination with super-resolution images, MitoLoc uses 3D reconstruction to calculate six geometrical classifiers which differentiate network morphologies in ∆fzo1, ∆ref2, and ∆dnm1 mutants, under oxidative stress and in cells lacking mtDNA, even when the network is fragmented to a similar extent. We find that mitochondrial fission and a decline in membrane potential do regularly, but not necessarily, co-occur. MitoLoc hence simplifies the measurement of mitochondrial membrane potential in parallel to detect morphological changes in mitochondrial networks. Marker plasmid open-source software as well as the mathematical procedures are made openly available.This work was supported by funding from the Wellcome Trust (RG 093735/Z/10/Z) and the ERC (Starting grant 260809). M.R. is a Wellcome Trust Research Career Development and Wellcome-Beit prize fellow.This is the final version. It was first published by Elsevier at http://www.sciencedirect.com/science/article/pii/S1567724915300088

Genetic control of the operculum and capsule morphology of Eucalyptus globulus

The petaline operculum that covers the inner whorls until anthesis and the woody capsule that develops after fertilization are reproductive structures of eucalypts that protect the flower and seeds. Although they are distinct organs, they both develop from flower buds and this common ontogeny suggests shared genetic control. In Eucalyptus globulus their morphology is variable and we aimed to identify the quantitative trait loci (QTL) underlying this variation and determine whether there is common genetic control of these ecologically and taxonomically important reproductive structures.EEA Bella VistaFil: Hernández, Mariano Agustín. University of Tasmania. School of Natural Sciences; Australia.Fil: Hernández, Mariano Agustín. University of Tasmania. ARC Training Centre for Forest Value; Australia.Fil: Hernández, Mariano Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentina.Fil: Butler, Jakob B. University of Tasmania. School of Natural Sciences; Australia.Fil: Ammitzboll, Hans. University of Tasmania. School of Natural Sciences; Australia.Fil: Ammitzboll, Hans. University of Tasmania. ARC Training Centre for Forest Value; Australia.Fil: Weller, James L. University of Tasmania. School of Natural Sciences; Australia.Fil: Weller, James L. Australian Research Council Centre of Excellence for Plant Success in Nature and Agriculture; Australia.Fil: Vaillancourt, René E. University of Tasmania. School of Natural Sciences; Australia.Fil: Vaillancourt, René E. University of Tasmania. ARC Training Centre for Forest Value; Australia.Fil: Potts, Brad M. University of Tasmania. School of Natural Sciences; Australia.Fil: Potts, Brad M. University of Tasmania. ARC Training Centre for Forest Value; Australia

Parentification: counselling talk on a helpline for children and young people

This chapter investigates counselling interactions where young clients talk about their experiences of taking on family responsibilities normatively associated with parental roles. In research counselling literature, practices where relationships in families operate so that there is a reversal of roles, with children managing the households and caring for parents and siblings, is described as parentification. Parentification is used in the counselling literature as a clinician/researcher term, which we ‘respecify’ (Garfinkel, 1991) the term by beginning with an investigation of young clients’ own accounts of being an adult or parent and how counsellors orient to these accounts. As well as providing understandings of how young people propose accounts of their experiences of adult-child role reversal, the chapter contributes to understanding how children and young people use the resources of counselling helplines, and how counsellors can communicate effectively with children and young people

Genetic variation in fire recovery and other fire‑related traits in a global eucalypt species

To understand the potential of forests to adapt to wildfire, we studied the genetic architecture of fire-related structural, damage and recovery traits in a globally important Australian forest tree species, Eucalyptus globulus. Fourteen traits were evaluated in an outcrossed F2 population in a field trial in Tasmania, Australia, which was burnt by a wildfire 14 years after planting. The trial also included open-pollinated families of the grandparental dwarf and tall ecotypes used to produce the F2 population. We studied the phenotypic correlations within the F2 population and performed quantitative trait loci (QTL) analyses using a linkage map comprised of 472 markers. Ecotype comparisons revealed that almost all traits were under genetic control, with trees of the dwarf ecotype significantly more damaged and mainly recovering from lignotubers, whereas tall ecotype trees mainly recovered from epicormic resprouts extending for a variable height up the stem. Within the F2, tree size was negatively correlated with fire damage and positively correlated with recovery. Genetic control of fire-related traits was confirmed by the detection of 38 QTL in the F2 population. These QTL accounted for 4 to 43% of the phenotypic variation in these traits. Several QTL co-located and likely reflect pleiotropic effects. However, many independent QTL were detected, including QTL for crown consumption and trunk scorch, epicormic resprouting, resprout herbivory, and seedling establishment. The QTL detected argue that many genetically controlled mechanisms are responsible for variation in fire damage and recovery.EEA Bella VistaFil: Hernández, Mariano Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; ArgentinaFil: Hernández, Mariano Agustín. University of Tasmania. School of Natural Sciences and ARC Training Centre for Forest Value; AustraliaFil: Butler, Jacob B. University of Tasmania. School of Natural Sciences and ARC Training Centre for Forest Value; AustraliaFil: Ammitzboll, Hans. University of Tasmania. School of Natural Sciences and ARC Training Centre for Forest Value; AustraliaFil: Freeman, Jules S. University of Tasmania. School of Natural Sciences and ARC Training Centre for Forest Value; AustraliaFil: Freeman, Jules S. Forest Genetics; Nueva ZelandaFil: O’Reilly‑Wapstra, Julianne. University of Tasmania. School of Natural Sciences and ARC Training Centre for Forest Value; AustraliaFil: Vaillancourt, René E. University of Tasmania. School of Natural Sciences and ARC Training Centre for Forest Value; AustraliaFil: Potts, Brad M. University of Tasmania. School of Natural Sciences and ARC Training Centre for Forest Value; Australi

Low-amplitude solar-like oscillations in the K5 V star ε\varepsilon Indi A

We have detected solar-like oscillations in the mid K-dwarf $\varepsilon$ Indi A, making it the coolest dwarf to have measured oscillations. The star is noteworthy for harboring a pair of brown dwarf companions and a Jupiter-type planet. We observed $\varepsilon$ Indi A during two radial velocity campaigns, using the high-resolution spectrographs HARPS (2011) and UVES (2021). Weighting the time series, we computed the power spectra and established the detection of solar-like oscillations with a power excess located at $5265 \pm 110 \ \mu$Hz -- the highest frequency solar-like oscillations so far measured in any star. The measurement of the center of the power excess allows us to compute a stellar mass of $0.782 \pm 0.023 \ M_\odot$ based on scaling relations and a known radius from interferometry. We also determine the amplitude of the peak power and note that there is a slight difference between the two observing campaigns, indicating a varying activity level. Overall, this work confirms that low-amplitude solar-like oscillations can be detected in mid-K type stars in radial velocity measurements obtained with high-precision spectrographs.Comment: 10 pages, 3 figures, accepted for publication in Ap

Measurement of (anti)deuteron and (anti)proton production in DIS at HERA

The first observation of (anti)deuterons in deep inelastic scattering at HERA has been made with the ZEUS detector at a centre-of-mass energy of 300--318 GeV using an integrated luminosity of 120 pb-1. The measurement was performed in the central rapidity region for transverse momentum per unit of mass in the range 0.3<p_T/M<0.7. The particle rates have been extracted and interpreted in terms of the coalescence model. The (anti)deuteron production yield is smaller than the (anti)proton yield by approximately three orders of magnitude, consistent with the world measurements.Comment: 26 pages, 9 figures, 5 tables, submitted to Nucl. Phys.

Clouds, circulation and climate sensitivity

Fundamental puzzles of climate science remain unsolved because of our limited understanding of how clouds, circulation and climate interact. One example is our inability to provide robust assessments of future global and regional climate changes. However, ongoing advances in our capacity to observe, simulate and conceptualize the climate system now make it possible to fill gaps in our knowledge. We argue that progress can be accelerated by focusing research on a handful of important scientific questions that have become tractable as a result of recent advances. We propose four such questions below; they involve understanding the role of cloud feedbacks and convective organization in climate, and the factors that control the position, the strength and the variability of the tropical rain belts and the extratropical storm tracks

Computer-Aided Solvent Screening for Biocatalysis

A computer-aidedsolventscreening methodology is described and tested for biocatalytic systems composed of enzyme, essential water and substrates/products dissolved in a solvent medium, without cells. The methodology is computationally simple, using group contribution methods for calculating constrained properties related to chemical reaction equilibrium, substrate and product solubility, water solubility, boiling points, toxicity and others. Two examples are provided, covering the screening of solvents for lipase-catalyzed transesterification of octanol and inulin with vinyl laurate. Esterification of acrylic acid with octanol is also addressed. Solvents are screened and candidates identified, confirming existing experimental results. Although the examples involve lipases, the method is quite general, so there seems to be no preclusion against application to other biocatalyst