Critical analysis of methods to determine growth, control and analysis of biofilms for potential non-submerged antibiofilm surfaces and coatings

The potential uses for antibiofilm surfaces reach across different sectors with significant resultant economic, societal and health impact. For those interested in using antibiofilm surfaces in the built environment, it is important that efficacy testing methods are relevant, reproducible and standardised where possible, to ensure data outputs are applicable to end-use, and comparable across the literature. Using pre-defined keywords, a review of literature reporting on antimicrobial surfaces (78 articles), within which a potential application was described as non-submerged/non-medical surface or coating with antibiofilm action, was undertaken. The most used methods utilized the growth of biofilm in submerged and static systems. Quantification varied (from most to least commonly used) across colony forming unit counts, non-microscopy fluorescence or spectroscopy, microscopy analysis, direct agar-contact, sequencing, and ELISA. Selection of growth media, microbial species, and incubation temperature also varied. In many cases, definitions of biofilm and attempts to quantify antibiofilm activity were absent or vague. Assessing a surface after biofilm recovery or assessing potential regrowth of a biofilm after initial analysis was almost entirely absent. It is clear the field would benefit from widely agreed and adopted approaches or guidance on how to select and incorporate end-use specific conditions, alongside minimum reporting guidelines may benefit the literature

Single-Electron Electronics

Contains table of contents for Section 2, research goals and objectives, a summary of recent work and a list of publications.Joint Services Electronics Program Contract DAAHO4-95-1-0038U.S. Army Research Office Grant DAAHO4-94-G-011

VLTI observations of the dust geometry around R Coronae Borealis stars

We are investigating the formation and evolution of dust around the hydrogen-deficient supergiants known as R Coronae Borealis (RCB) stars. We aim to determine the connection between the probable merger past of these stars and their current dust-production activities. We carried out high-angular resolution interferometric observations of three RCB stars, namely RY Sgr, V CrA, and V854 Cen with the mid-IR interferometer, MIDI on the VLTI, using two telescope pairs. The baselines ranged from 30 to 60 m, allowing us to probe the dusty environment at very small spatial scales (~ 50 mas or 400 stellar radii). The observations of the RCB star dust environments were interpreted using both geometrical models and one-dimensional radiative transfer codes. From our analysis we find that asymmetric circumstellar material is apparent in RY Sgr, may also exist in V CrA, and is possible for V854 Cen. Overall, we find that our observations are consistent with dust forming in clumps ejected randomly around the RCB star so that over time they create a spherically symmetric distribution of dust. However, we conclude that the determination of whether there is a preferred plane of dust ejection must wait until a time series of observations are obtained.Comment: Accepted for publication in MNRAS; 14 pages, 10 figures, 6 table

Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates

Supplementary data to this article can be found online at https://doi.org/10.1016/j.bioflm.2019.100010.The lack of reproducibility of published studies is one of the major issues facing the scientific community, and the field of biofilm microbiology has been no exception. One effective strategy against this multifaceted problem is the use of minimum information guidelines. This strategy provides a guide for authors and reviewers on the necessary information that a manuscript should include for the experiments in a study to be clearly interpreted and independently reproduced. As a result of several discussions between international groups working in the area of biofilms, we present a guideline for the spectrophotometric and fluorometric assessment of biofilm formation in microplates. This guideline has been divided into 5 main sections, each presenting a comprehensive set of recommendations. The intention of the minimum information guideline is to improve the quality of scientific communication that will augment interlaboratory reproducibility in biofilm microplate assays.This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska – Curie grant agreement No 722467, as part of the Print-Aid consortium. The information and views set out in this article are those of the authors and do not necessarily reflect the official opinion of the European Union. Neither the European Union institutions and bodies nor any person acting on their behalf may be held responsible for the use which may be made of the information contained therein. This work received additional financial support by: project UID/EQU/00511/2019 - Laboratory for Process Engineering, Environment, Biotechnology and Energy – LEPABE funded by national funds through FCT/MCTES (PIDDAC); Project “LEPABE-2-ECO-INNOVATION” – NORTE-01-0145-FEDER-000005, funded by Norte Portugal Regional Operational Programme (NORTE 2020), under PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF).info:eu-repo/semantics/publishedVersio

Interference in interacting quantum dots with spin

We study spectral and transport properties of interacting quantum dots with spin. Two particular model systems are investigated: Lateral multilevel and two parallel quantum dots. In both cases different paths through the system can give rise to interference. We demonstrate that this strengthens the multilevel Kondo effect for which a simple two-stage mechanism is proposed. In parallel dots we show under which conditions the peak of an interference-induced orbital Kondo effect can be split.Comment: 8 pages, 8 figure

Interlaboratory study for the evaluation of three microtiter plate-based biofilm quantification methods

Microtiter plate methods are commonly used for biofilm assessment. However, results obtained with these methods have often been difficult to reproduce. Hence, it is important to obtain a better understanding of the repeatability and reproducibility of these methods. An interlaboratory study was performed in five different laboratories to evaluate the reproducibility and responsiveness of three methods to quantify Staphylococcus aureus biofilm formation in 96-well microtiter plates: crystal violet, resazurin, and plate counts. An inter-lab protocol was developed for the study. The protocol was separated into three steps: biofilm growth, biofilm challenge, biofilm assessment. For control experiments participants performed the growth and assessment steps only. For treatment experiments, all three steps were performed and the efficacy of sodium hypochlorite (NaOCl) in killing S. aureus biofilms was evaluated. In control experiments, on the log(10)-scale, the reproducibility SD (S-R) was 0.44 for crystal violet, 0.53 for resazurin, and 0.92 for the plate counts. In the treatment experiments, plate counts had the best responsiveness to different levels of efficacy and also the best reproducibility with respect to responsiveness (Slope/S-R=1.02), making it the more reliable method to use in an antimicrobial efficacy test. This study showed that the microtiter plate is a versatile and easy-to-use biofilm reactor, which exhibits good repeatability and reproducibility for different types of assessment methods, as long as a suitable experimental design and statistical analysis is applied.Peer reviewe

The influence of binarity on dust obscuration events in the planetary nebula M 2-29 and its analogues

The central star of the planetary nebula (CSPN) M 2-29 shows an extraordinary R Coronae Borealis-like fading event in its optical lightcurve. The only other CSPN to show these events are CPD-568032 (Hen 3-1333) and V651 Mon (NGC 2346). Dust cloud formation in the line of sight appears responsible but the exact triggering mechanism is not well understood. Understanding how planetary nebulae (PNe) trigger dust obscuration events may help understand the same process in a wide range of objects including Population-I WC9 stars, symbiotic stars and perhaps Asymptotic Giant Branch (AGB) stars with long secondary periods (LSPs). A binary scenario involving an eccentric, wide companion that triggers dust formation via interaction at periastron is a potential explanation that has been suggested for LSP variables. Model fits to the lightcurves of CPD-568032 and M 2-29 show the dust forms in excess of 70 AU at the inner edge of a dust disk. In the case of CPD-568032 this radius is far too large to coincide with a binary companion trigger, although a binary may have been responsible for the formation of the dust disk. We find no direct evidence to support previous claims of binarity in M 2-29 either from the OGLE lightcurve or deep medium-resolution VLT FLAMES spectroscopy of the CSPN. We classify the CSPN as Of(H) with T_eff=50+-10 kK and log g=4.0+-0.3. We find a mean distance of 7.4+-1.8 kpc to M 2-29 at which the M_V=-0.9 mag CSPN could potentially hide a subgiant luminosity or fainter companion. A companion would help explain the multiple similarities with D'-type symbiotic stars whose outer nebulae are thought to be bona-fide PNe. The 7.4 kpc distance, oxygen abundance of 8.3 dex and Galactic coordinates (l=4.0, b=-3.0) prove that M 2-29 is a Galactic Bulge PN and not a Halo PN as commonly misconceived.Comment: 15 pages, 14 figures, 7 tables. Accepted for publication in A\&

Infrared Space Observatory Spectra of R Coronae Borealis Stars. I. Emission Features in the Interval 3 - 25 microns

Infrared Space Observatory 3 - 25 $\mu$m spectra of the R Coronae Borealis stars V854 Cen, R CrB, and RY Sgr are presented and discussed. Sharp emission features coincident in wavelengths with the well known Unidentified Emission Features are present in the spectrum of V854 Cen but not of R CrB or RY Sgr. Since V854 Cen is not particularly H-poor and has a 1000 times more H than the other stars, the emission features are probably from a carrier containing hydrogen. There is a correspondence between the features and emission from laboratory samples of hydrogenated amorphous carbon. A search for C$_{60}$ in emission or absorption proved negative. Amorphous carbon particles account for the broad emission features seen between 6 - 14 $\mu$m in the spectrum of each star.Comment: 11 pages with 2 tables and 4 figures in ApJ print page form. Accepted for publication in Ap

Detection of an asymmetry in the envelope of the carbon Mira R Fornacis using VLTI/MIDI

Aims. We present a study of the envelope morphology of the carbon Mira R For with VLTI/MIDI. This object is one of the few asymptotic giant branch (AGB) stars that underwent a dust-obscuration event. The cause of such events is still a matter of discussion. Several symmetric and asymmetric scenarios have been suggested in the literature. Methods. Mid-infrared interferometric observations were obtained separated by two years. The observations probe different depths of the atmosphere and cover different pulsation phases. The visibilities and the differential phases were interpreted using GEM-FIND, a tool for fitting spectrally dispersed interferometric observations with the help of wavelength-dependent geometric models. Results. We report the detection of an asymmetric structure revealed through the MIDI differential phase. This asymmetry is observed at the same baseline and position angle two years later. The observations are best simulated with a model that includes a uniform-disc plus a Gaussian envelope plus a point-source. The geometric model can reproduce both the visibilities and the differential phase signatures. Conclusions. Our MIDI data favour explanations of the R For obscuration event that are based on an asymmetric geometry. We clearly detect a photocentre shift between the star and the strongly resolved dust component. This might be caused by a dust clump or a substellar companion. However, the available observations do not allow us to distinguish between the two options. The finding has strong implications for future studies of the geometry of the envelope of AGB stars: if this is a binary, are all AGB stars that show an obscuration event binaries as well? Or are we looking at asymmetric mass-loss processes (i.e. dusty clumps) in the inner part of a carbon-rich Mira?Comment: accepted for publication as A&A lette