Sedimentological analysis of marine Pleistocene deposits of southeastern Tunisia: evidence of two positive eustatic pulsations during the marine isotopic substage 5e (Eemian, Tyrrhenian)

International audienceAbstract Detailed sedimentological and petrographic analysis of marine Pleistocene deposits along the coastal area of southeastern Tunisia allow to identify two distinct lithostratigraphic units separated by an erosion surface (fig. 2 et 5 A and B). These two commonly superposed units form a ridge parallel to the coast. The palaeocoastal morphology was more irregular than the present-day coastline with areas either more protected or more exposed than now (fig. 3). The lower unit overlies an erosion surface cutting into Mio-Pliocene and Villafranchian deposits. It consists of fine-grained bioclastic quartz-rich sands (fig. 5 C), locally overlain by thin marl layers containing benthic foraminifera and ostracods. The very fine facies (silts and clays) represent relatively protected areas while the coarser facies developed in the more exposed zones. These deposits locally display a well-developed aeolian facies that terminates the sedimentary sequence. This unit, well developed in Jerba Island and Jorf peninsula, strongly resembles the stratigraphic unit of “Khnis” as defined by Mahmoudi [1988] on the coast of Central Tunisia. The upper unit is the better developed in the studied area. It consists of carbonate deposits composed mainly by ooids and peloids (fig. 5 D) and contains also a warm Senegalese fauna, especially Strombus. Locally, in the exposed areas, it shows a coarse facies which resulted mainly from the erosion of the calcareous Villafranchian deposits. This unit displays a remarkable shallowing-upward sequence from shoreface to aeolian dunes (fig. 5 E and F). It constitutes the lateral equivalent of the stratigraphic unit of “Réjiche” as defined by Mahmoudi [1988] in Central Tunisia. These two units, called in this study “quartz-rich unit” (the lower sequence) and “carbonate unit” (the upper one), developed during two distinctive sedimentation phases associated with two sea-level highstands separated by a marine regression. During the first highstand sea-level was about 3 m higher than today whereas it was at about +5 m during the second highstand [Jedoui, 2000]. Along the Mediterranean coasts the Strombus paleobeaches, which are contemporary with the carbonate unit, are well developed and traditionally called Tyrrhenian deposits. Their radiochemical dating, using corals, gives ages of about 125 ka [Hearty et al., 1986 ; Miller et al., 1986 ; Dumas et al., 1991 ; Vai et Pasini, 1996]. We obtained the most reliable uranium/thorium dates in southeastern Tunisia on oyster shells. Results show that the two units developed during the marine isotopic substage 5e [last interglacial ; Jedoui, 2000]. This evidence suggests that substage 5e was characterised by at least two eustatic maxima separated by a lowering of sea level during a marine regression. Our results are therefore in agreement with recent palaeoclimatic reconstructions and in particular with sea level reconstructions and marine oxygen isotope records that indicate the distinct possibility of two positive eustatic pulsations during the last interglacial [Hillaire-Marcel et al., 1996 ; Kindler et al., 1997 ; Plaziat et al., 1998]. Variations in the petrographic content of the two outlined units reflect drastic palaeoclimatic fluctuations in southeastern Tunisia during the last interglacial. The establishment of wetter climatic conditions at the beginning of marine isotopic substage 5e than today was responsible for an enhanced terrigenous materiel supply from the continent as showed by siliciclastic sedimentation along the coast (lower unit). Our data are in agreement with the strong sea surface salinity lowering observed in Mediterranean basins at the beginning of the last interglacial period [Kallel et al., 2000]. The regression of these conditions during the second half of the last interglacial favoured a carbonate sedimentation (upper unit) in southeastern Tunisia

Inhibition of Wnt/β-Catenin Signaling by a Soluble Collagen-Derived Frizzled Domain Interacting with Wnt3a and the Receptors Frizzled 1 and 8

The Wnt/β-catenin pathway controls cell proliferation, death and differentiation. Several families of extracellular proteins can antagonize Wnt/β-catenin signaling, including the decoy receptors known as secreted frizzled related proteins (SFRPs), which have a cysteine-rich domain (CRD) structurally similar to the extracellular Wnt-binding domain of the frizzled receptors. SFRPs inhibit Wnt signaling by sequestering Wnts through the CRD or by forming inactive complexes with the frizzled receptors. Other endogenous molecules carrying frizzled CRDs inhibit Wnt signaling, such as V3Nter, which is proteolytically derived from the cell surface component collagen XVIII and contains a biologically active frizzled domain (FZC18) inhibiting in vivo cell proliferation and tumor growth in mice. We recently showed that FZC18 expressing cells deliver short-range signals to neighboring cells, decreasing their proliferation in vitro and in vivo through the Wnt/β-catenin signaling pathway. Here, using low concentrations of soluble FZC18 and Wnt3a, we show that they physically interact in a cell-free system. In addition, soluble FZC18 binds the frizzled 1 and 8 receptors' CRDs, reducing cell sensitivity to Wnt3a. Conversely, inhibition of Wnt/β-catenin signaling was partially rescued by the expression of full-length frizzled 1 and 8 receptors, but enhanced by the expression of a chimeric cell-membrane-tethered frizzled 8 CRD. Moreover, soluble, partially purified recombinant FZC18_CRD inhibited Wnt3a-induced β-catenin activation. Taken together, the data indicate that collagen XVIII-derived frizzled CRD shifts Wnt sensitivity of normal cells to a lower pitch and controls their growth

Pooled analysis of iron-related genes in Parkinson's disease: Association with transferrin

Pathologic features of Parkinson's disease (PD) include death of dopaminergic neurons in the substantia nigra, presence of α-synuclein containing Lewy bodies, and iron accumulation in PD-related brain regions. The observed iron accumulation may be contributing to PD etiology but it also may be a byproduct of cell death or cellular dysfunction. To elucidate the possible role of iron accumulation in PD, we investigated genetic variation in 16 genes related to iron homeostasis in three case-control studies from the United States, Australia, and France. After screening 90 haplotype tagging single nucleotide polymorphisms (SNPs) within the genes of interest in the US study population, we investigated the five most promising gene regions in two additional independent case-control studies. For the pooled data set (1289 cases, 1391 controls) we observed a protective association (OR. = 0.83, 95% CI: 0.71-0.96) between PD and a haplotype composed of the A allele at rs1880669 and the T allele at rs1049296 in transferrin (TF; GeneID: 7018). Additionally, we observed a suggestive protective association (OR. = 0.87, 95% CI: 0.74-1.02) between PD and a haplotype composed of the G allele at rs10247962 and the A allele at rs4434553 in transferrin receptor 2 (TFR2; GeneID: 7036). We observed no associations in our pooled sample for haplotypes in SLC40A1, CYB561, or HFE. Taken together with previous findings in model systems, our results suggest that TF or a TF- TFR2 complex may have a role in the etiology of PD, possibly through iron misregulation or mitochondrial dysfunction within dopaminergic neurons

Nck2 promotes human melanoma cell proliferation, migration and invasion in vitro and primary melanoma-derived tumor growth in vivo

<p>Abstract</p> <p>Background</p> <p>Nck1 and Nck2 adaptor proteins are involved in signaling pathways mediating proliferation, cytoskeleton organization and integrated stress response. Overexpression of Nck1 in fibroblasts has been shown to be oncogenic. Through the years this concept has been challenged and the consensus is now that overexpression of either Nck cooperates with strong oncogenes to transform cells. Therefore, variations in Nck expression levels in transformed cells could endorse cancer progression.</p> <p>Methods</p> <p>Expression of Nck1 and Nck2 proteins in various cancer cell lines at different stages of progression were analyzed by western blots. We created human primary melanoma cell lines overexpressing GFP-Nck2 and investigated their ability to proliferate along with metastatic characteristics such as migration and invasion. By western blot analysis, we compared levels of proteins phosphorylated on tyrosine as well as cadherins and integrins in human melanoma cells overexpressing or not Nck2. Finally, in mice we assessed tumor growth rate of human melanoma cells expressing increasing levels of Nck2.</p> <p>Results</p> <p>We found that expression of Nck2 is consistently increased in various metastatic cancer cell lines compared with primary counterparts. Particularly, we observed significant higher levels of Nck2 protein and mRNA, as opposed to no change in Nck1, in human metastatic melanoma cell lines compared with non-metastatic melanoma and normal melanocytes. We demonstrated the involvement of Nck2 in proliferation, migration and invasion in human melanoma cells. Moreover, we discovered that Nck2 overexpression in human primary melanoma cells correlates with higher levels of proteins phosphorylated on tyrosine residues, assembly of Nck2-dependent pY-proteins-containing molecular complexes and downregulation of cadherins and integrins. Importantly, we uncovered that injection of Nck2-overexpressing human primary melanoma cells into mice increases melanoma-derived tumor growth rate.</p> <p>Conclusions</p> <p>Collectively, our data indicate that Nck2 effectively influences human melanoma phenotype progression. At the molecular level, we propose that Nck2 in human primary melanoma promotes the formation of molecular complexes regulating proliferation and actin cytoskeleton dynamics by modulating kinases or phosphatases activities that results in increased levels of proteins phosphorylated on tyrosine residues. This study provides new insights regarding cancer progression that could impact on the therapeutic strategies targeting cancer.</p

Structural Basis for Fe–S Cluster Assembly and tRNA Thiolation Mediated by IscS Protein–Protein Interactions

Crystal structures reveal how distinct sites on the cysteine desulfurase IscS bind two different sulfur-acceptor proteins, IscU and TusA, to transfer sulfur atoms for iron-sulfur cluster biosynthesis and tRNA thiolation

A Cryptic Frizzled Module in Cell Surface Collagen 18 Inhibits Wnt/β−Catenin Signaling

Collagens contain cryptic polypeptide modules that regulate major cell functions, such as cell proliferation or death. Collagen XVIII (C18) exists as three amino terminal end variants with specific amino terminal polypeptide modules. We investigated the function of the variant 3 of C18 (V3C18) containing a frizzled module (FZC18), which carries structural identity with the extracellular cysteine-rich domain of the frizzled receptors. We show that V3C18 is a cell surface heparan sulfate proteoglycan, its topology being mediated by the FZC18 module. V3C18 mRNA was expressed at low levels in 21 normal adult human tissues. Its expression was up-regulated in fibrogenesis and in small well-differentiated liver tumors, but decreased in advanced human liver cancers. Low FZC18 immunostaining in liver cancer nodules correlated with markers of high Wnt/β−catenin activity. V3C18 (Mr = 170 kD) was proteolytically processed into a cell surface FZC18-containing 50 kD glycoprotein precursor that bound Wnt3a in vitro through FZC18 and suppressed Wnt3a-induced stabilization of β−catenin. Ectopic expression of either FZC18 (35 kD) or its 50 kD precursor inhibited Wnt/β−catenin signaling in colorectal and liver cancer cell lines, thus downregulating major cell cycle checkpoint gatekeepers cyclin D1 and c-myc and reducing tumor cell growth. By contrast, full-length V3C18 was unable to inhibit Wnt signaling. In summary, we identified a cell-surface signaling pathway whereby FZC18 inhibits Wnt/β−catenin signaling. The signal, encrypted within cell-surface C18, is released by enzymatic processing as an active frizzled cysteine-rich domain (CRD) that reduces cancer cell growth. Thus, extracellular matrix controls Wnt signaling through a collagen-embedded CRD behaving as a cell-surface sensor of proteolysis, conveying feedback cues to control cancer cell fate

Gold-induced crystallization of amorphous germanium deposited on glass : bilayer and multilayer films

International audienc

Approches par télédétection et cartographie des espaces sahéliens mauritaniens

National audienceLa période de sécheresse de la fin du 20ème siècle a touché les régions sahéliennes et engendré une dégradation du milieu et des ressources avec pour conséquence une réduction du cheptel et un appauvrissement des populations pastorales. Depuis la fin des années 90, la partie occidentale de ce domaine connaît une amélioration des conditions pluviométriques susceptibles de créer des conditions favorables à la régénération des pâturages. Grâce à une étude diachronique sur une période de 50 ans (1956 - 2005), notre objectif est d'estimer les réponses du milieu au forçage pluviométrique en identifiant l'évolution du rapport entre les aires végétalisées et les aires sableuses. Les études sont centrées sur un secteur de la région sud - ouest du Hodh mauritanien caractérisé par des géosystèmes sableux dominants façonnés en cordons dunaires et une homogénéité du mode d'utilisation de l'espace. La période considérée couvre la fin d'une phase d'années humides, une phase sèche de 1970 à 1999 et la reprise de précipitations plus abondantes depuis 1999, soit deux changements majeurs dans les totaux pluviométriques enregistrés. La démarche utilisée est fondée sur des méthodes et techniques bâties sur l'analyse des données de télédétection spatiale. Elle repose sur une approche systémique permettant de mettre en relation les composantes du milieu à partir de traitements adaptés à l'identification et l'extraction des aires sableuses et végétalisées, au calcul des superficies affectées par la régression végétale et à la mise en relation des différentes organisations biogéomorphologiques. Les études ont été menées à l'aide d'une couverture photographique aérienne (1956, état de référence) et d'images satellite Landsat (1988, 1999, 2001) et Spot (2005) acquises aux périodes les plus favorables à la séparabilité spectrale des objets, complétées par cinq missions terrain effectuées entre 2005 et 2009. Cette démarche nous a conduit à souligner la combinaison complexe de systèmes fonctionnels distincts où les variables d'état et les variables dynamiques interagissent pour engendrer les mécanismes et processus physiques et biologiques. La spatialisation des processus, l'organisation générale et les changements paysagers ont été traduits sous formes cartographiques géoréférencées à l'échelle de la région et des sous-unités fonctionnelles. Les résultats montrent une moindre efficacité des processus éoliens laissant plus de place aux processus biologiques favorables à la reconquête des sols sableux par la végétation

Investigation on the Regional Loss Factor and Its Anisotropy for Aortic Aneurysms

An aortic aneurysm is a lethal arterial disease that mainly occurs in the thoracic and abdominal regions of the aorta. Thoracic aortic aneurysms are prevalent in the root/ascending parts of the aorta and can lead to aortic rupture resulting in the sudden death of patients. Understanding the biomechanical and histopathological changes associated with ascending thoracic aortic aneurysms (ATAAs), this study investigates the mechanical properties of the aorta during strip-biaxial tensile cycles. The loss factor—defined as the ratio of dissipated energy to the energy absorbed during a tensile cycle—the incremental modulus, and their anisotropy indexes were compared with the media fiber compositions for aneurysmal (n = 26) and control (n = 4) human ascending aortas. The aneurysmal aortas were categorized into the aortas with bicuspid aortic valves (BAV) and tricuspid aortic valves (TAV). The strip-biaxial loss factor correlates well with the diameter of the aortas with BAV and TAV (for the axial direction, respectively, R2 = 0.71, p = 0.0022 and R2 = 0.54, p = 0.0096). The loss factor increases significantly with patients’ age in the BAV group (for the axial direction: R2 = 0.45, p = 0.0164). The loss factor is isotropic for all TAV quadrants, whereas it is on average only isotropic in the anterior and outer curvature regions of the BAV group. The results suggest that loss factor may be a useful surrogate measure to describe the histopathology of aneurysmal tissue and to demonstrate the differences between ATAAs with the BAV and TAV

Biomass production and nutritive value of Kenaf (Hibiscus cannabinus) at various stages of growth

8 páginas, 2 tablas, 1 figura.This study has investigated the chemical composition and in vitro digestibility of leaves and stems of Kenaf (Hibiscus cannabinus L.) harvested throughout the growing season. Kenaf (‘Everglades 41’) was planted in June 2011 and harvested at 25, 50 and 75 days after planting (DAP). Crude protein (CP) in leaves was more (P < 0.01) than double than in stems harvested at the same growth stage. Protein concentration in leaves decreased (P < 0.01) as harvesting date was delayed from 50 to 75 DAP, whereas no significant changes in CP were detected in mature stems (75 DAP) as compared to those collected at an earlier stage (50 DAP). Regardless of the maturity stage, cell wall content (NDF and ADF) was higher (P < 0.05) in stems than in leaves. Cell walls were more lignified (P < 0.05) in stems harvested at 50 and 75 DAP and mature leaves (75 DAP) than in young leaves harvested at 25 and 50 DAP. True in vitro dry matter digestibility was lowest (P < 0.05) in mature stems (62%) and highest in leaves harvested at 50 DAP (93%). Conclusively, Kenaf can be grown during the summer season and harvested as a relatively high-quality forage (with high protein content and in vitro digestibility) as early as 50 DAP